Experiment: L-Serine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS00815 and EX31_RS00820 are separated by 199 nucleotides EX31_RS00820 and EX31_RS00825 are separated by 33 nucleotides EX31_RS00825 and EX31_RS00830 overlap by 4 nucleotides
EX31_RS00815: EX31_RS00815 - alpha/beta hydrolase, at 177,847 to 178,860
_RS00815
EX31_RS00820: EX31_RS00820 - VOC family protein, at 179,060 to 179,899
_RS00820
EX31_RS00825: EX31_RS00825 - ABC transporter ATP-binding protein, at 179,933 to 180,652
_RS00825
EX31_RS00830: EX31_RS00830 - ABC transporter ATP-binding protein, at 180,649 to 181,428
_RS00830
Position (kb)
179
180 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 178.131 kb on + strand, within EX31_RS00815 at 178.132 kb on - strand, within EX31_RS00815 at 178.132 kb on - strand, within EX31_RS00815 at 178.132 kb on - strand, within EX31_RS00815 at 178.132 kb on - strand, within EX31_RS00815 at 178.197 kb on + strand, within EX31_RS00815 at 178.197 kb on + strand, within EX31_RS00815 at 178.198 kb on - strand, within EX31_RS00815 at 178.328 kb on + strand, within EX31_RS00815 at 178.328 kb on + strand, within EX31_RS00815 at 178.328 kb on + strand, within EX31_RS00815 at 178.328 kb on + strand, within EX31_RS00815 at 178.328 kb on + strand, within EX31_RS00815 at 178.328 kb on + strand, within EX31_RS00815 at 178.329 kb on - strand, within EX31_RS00815 at 178.329 kb on - strand, within EX31_RS00815 at 178.329 kb on - strand, within EX31_RS00815 at 178.329 kb on - strand, within EX31_RS00815 at 178.331 kb on + strand, within EX31_RS00815 at 178.331 kb on + strand, within EX31_RS00815 at 178.332 kb on - strand, within EX31_RS00815 at 178.332 kb on - strand, within EX31_RS00815 at 178.332 kb on - strand, within EX31_RS00815 at 178.332 kb on - strand, within EX31_RS00815 at 178.332 kb on - strand, within EX31_RS00815 at 178.332 kb on - strand, within EX31_RS00815 at 178.333 kb on + strand, within EX31_RS00815 at 178.333 kb on + strand, within EX31_RS00815 at 178.334 kb on - strand, within EX31_RS00815 at 178.443 kb on - strand, within EX31_RS00815 at 178.543 kb on - strand, within EX31_RS00815 at 178.549 kb on - strand, within EX31_RS00815 at 178.623 kb on + strand, within EX31_RS00815 at 178.623 kb on + strand, within EX31_RS00815 at 178.624 kb on - strand, within EX31_RS00815 at 178.624 kb on - strand, within EX31_RS00815 at 178.624 kb on - strand, within EX31_RS00815 at 178.625 kb on + strand, within EX31_RS00815 at 178.625 kb on + strand, within EX31_RS00815 at 178.626 kb on - strand, within EX31_RS00815 at 178.670 kb on - strand, within EX31_RS00815 at 178.707 kb on + strand, within EX31_RS00815 at 178.707 kb on + strand, within EX31_RS00815 at 178.708 kb on - strand, within EX31_RS00815 at 178.735 kb on - strand, within EX31_RS00815 at 178.735 kb on - strand, within EX31_RS00815 at 178.737 kb on + strand, within EX31_RS00815 at 178.738 kb on - strand, within EX31_RS00815 at 178.753 kb on - strand, within EX31_RS00815 at 178.862 kb on + strand at 178.863 kb on - strand at 178.863 kb on - strand at 178.863 kb on - strand at 178.863 kb on - strand at 178.864 kb on + strand at 178.888 kb on - strand at 178.918 kb on - strand at 178.918 kb on - strand at 179.020 kb on + strand at 179.020 kb on + strand at 179.032 kb on - strand at 179.041 kb on - strand at 179.061 kb on + strand at 179.061 kb on + strand at 179.062 kb on - strand at 179.187 kb on - strand, within EX31_RS00820 at 179.278 kb on + strand, within EX31_RS00820 at 179.279 kb on - strand, within EX31_RS00820 at 179.310 kb on + strand, within EX31_RS00820 at 179.310 kb on + strand, within EX31_RS00820 at 179.311 kb on - strand, within EX31_RS00820 at 179.426 kb on - strand, within EX31_RS00820 at 179.742 kb on + strand, within EX31_RS00820 at 179.743 kb on - strand, within EX31_RS00820 at 179.743 kb on - strand, within EX31_RS00820 at 179.743 kb on - strand, within EX31_RS00820 at 179.747 kb on - strand, within EX31_RS00820 at 179.864 kb on - strand at 179.899 kb on + strand at 179.900 kb on - strand at 179.900 kb on - strand at 179.900 kb on - strand at 179.955 kb on + strand at 179.956 kb on - strand at 179.982 kb on - strand at 180.033 kb on + strand, within EX31_RS00825 at 180.033 kb on + strand, within EX31_RS00825 at 180.033 kb on + strand, within EX31_RS00825 at 180.034 kb on - strand, within EX31_RS00825 at 180.034 kb on - strand, within EX31_RS00825 at 180.064 kb on + strand, within EX31_RS00825 at 180.064 kb on + strand, within EX31_RS00825 at 180.120 kb on - strand, within EX31_RS00825 at 180.185 kb on + strand, within EX31_RS00825 at 180.186 kb on - strand, within EX31_RS00825 at 180.186 kb on - strand, within EX31_RS00825 at 180.275 kb on - strand, within EX31_RS00825 at 180.319 kb on + strand, within EX31_RS00825 at 180.319 kb on + strand, within EX31_RS00825 at 180.319 kb on + strand, within EX31_RS00825 at 180.319 kb on + strand, within EX31_RS00825 at 180.319 kb on + strand, within EX31_RS00825 at 180.320 kb on - strand, within EX31_RS00825 at 180.320 kb on - strand, within EX31_RS00825 at 180.320 kb on - strand, within EX31_RS00825 at 180.320 kb on - strand, within EX31_RS00825 at 180.320 kb on - strand, within EX31_RS00825 at 180.593 kb on - strand at 180.593 kb on - strand at 180.622 kb on - strand at 180.647 kb on - strand at 180.689 kb on + strand at 180.689 kb on + strand at 180.690 kb on - strand at 180.690 kb on - strand at 180.690 kb on - strand at 180.690 kb on - strand at 180.690 kb on - strand at 180.701 kb on - strand at 180.793 kb on - strand, within EX31_RS00830 at 180.802 kb on - strand, within EX31_RS00830 at 180.829 kb on - strand, within EX31_RS00830 at 180.874 kb on + strand, within EX31_RS00830 at 180.875 kb on - strand, within EX31_RS00830 at 180.875 kb on - strand, within EX31_RS00830
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); ShewMM media; no shaking remove 178,131 + EX31_RS00815 0.28 -0.8 178,132 - EX31_RS00815 0.28 +0.7 178,132 - EX31_RS00815 0.28 -1.0 178,132 - EX31_RS00815 0.28 +0.5 178,132 - EX31_RS00815 0.28 +0.1 178,197 + EX31_RS00815 0.35 +0.3 178,197 + EX31_RS00815 0.35 +1.3 178,198 - EX31_RS00815 0.35 +1.1 178,328 + EX31_RS00815 0.47 -2.3 178,328 + EX31_RS00815 0.47 -0.3 178,328 + EX31_RS00815 0.47 +0.5 178,328 + EX31_RS00815 0.47 +0.6 178,328 + EX31_RS00815 0.47 -1.2 178,328 + EX31_RS00815 0.47 +1.3 178,329 - EX31_RS00815 0.48 +1.3 178,329 - EX31_RS00815 0.48 +0.6 178,329 - EX31_RS00815 0.48 -0.4 178,329 - EX31_RS00815 0.48 -0.3 178,331 + EX31_RS00815 0.48 -0.4 178,331 + EX31_RS00815 0.48 -0.7 178,332 - EX31_RS00815 0.48 +0.7 178,332 - EX31_RS00815 0.48 +1.7 178,332 - EX31_RS00815 0.48 +1.3 178,332 - EX31_RS00815 0.48 -0.8 178,332 - EX31_RS00815 0.48 -0.5 178,332 - EX31_RS00815 0.48 -0.7 178,333 + EX31_RS00815 0.48 -0.7 178,333 + EX31_RS00815 0.48 +0.0 178,334 - EX31_RS00815 0.48 -1.7 178,443 - EX31_RS00815 0.59 +0.6 178,543 - EX31_RS00815 0.69 -0.2 178,549 - EX31_RS00815 0.69 -0.2 178,623 + EX31_RS00815 0.77 -0.4 178,623 + EX31_RS00815 0.77 +1.8 178,624 - EX31_RS00815 0.77 +0.8 178,624 - EX31_RS00815 0.77 -2.2 178,624 - EX31_RS00815 0.77 +0.7 178,625 + EX31_RS00815 0.77 +0.5 178,625 + EX31_RS00815 0.77 +1.8 178,626 - EX31_RS00815 0.77 +1.3 178,670 - EX31_RS00815 0.81 +1.2 178,707 + EX31_RS00815 0.85 -0.2 178,707 + EX31_RS00815 0.85 -0.0 178,708 - EX31_RS00815 0.85 -0.8 178,735 - EX31_RS00815 0.88 -0.2 178,735 - EX31_RS00815 0.88 -0.4 178,737 + EX31_RS00815 0.88 +0.9 178,738 - EX31_RS00815 0.88 -2.1 178,753 - EX31_RS00815 0.89 -0.5 178,862 + +0.6 178,863 - -0.4 178,863 - -0.1 178,863 - +1.1 178,863 - +0.2 178,864 + -0.4 178,888 - +2.8 178,918 - +2.0 178,918 - +2.2 179,020 + -0.2 179,020 + +1.4 179,032 - -1.2 179,041 - +1.3 179,061 + -1.6 179,061 + -0.1 179,062 - -1.9 179,187 - EX31_RS00820 0.15 -0.2 179,278 + EX31_RS00820 0.26 +1.2 179,279 - EX31_RS00820 0.26 -0.8 179,310 + EX31_RS00820 0.30 +1.6 179,310 + EX31_RS00820 0.30 -0.7 179,311 - EX31_RS00820 0.30 -0.6 179,426 - EX31_RS00820 0.44 -1.7 179,742 + EX31_RS00820 0.81 -1.7 179,743 - EX31_RS00820 0.81 -1.4 179,743 - EX31_RS00820 0.81 -0.1 179,743 - EX31_RS00820 0.81 +0.3 179,747 - EX31_RS00820 0.82 -0.2 179,864 - +1.2 179,899 + +0.2 179,900 - -1.4 179,900 - -1.5 179,900 - -0.5 179,955 + +1.9 179,956 - -0.0 179,982 - -1.2 180,033 + EX31_RS00825 0.14 +0.5 180,033 + EX31_RS00825 0.14 -1.0 180,033 + EX31_RS00825 0.14 -1.5 180,034 - EX31_RS00825 0.14 +2.2 180,034 - EX31_RS00825 0.14 +0.3 180,064 + EX31_RS00825 0.18 -1.2 180,064 + EX31_RS00825 0.18 +0.5 180,120 - EX31_RS00825 0.26 -0.2 180,185 + EX31_RS00825 0.35 +0.8 180,186 - EX31_RS00825 0.35 -0.6 180,186 - EX31_RS00825 0.35 +1.8 180,275 - EX31_RS00825 0.47 +0.2 180,319 + EX31_RS00825 0.54 +1.0 180,319 + EX31_RS00825 0.54 -0.7 180,319 + EX31_RS00825 0.54 +1.6 180,319 + EX31_RS00825 0.54 -0.8 180,319 + EX31_RS00825 0.54 -1.7 180,320 - EX31_RS00825 0.54 +1.3 180,320 - EX31_RS00825 0.54 +0.5 180,320 - EX31_RS00825 0.54 +1.9 180,320 - EX31_RS00825 0.54 -1.6 180,320 - EX31_RS00825 0.54 -1.3 180,593 - +0.2 180,593 - +1.2 180,622 - -1.4 180,647 - -1.1 180,689 + +1.2 180,689 + +1.4 180,690 - -0.0 180,690 - +1.0 180,690 - +0.7 180,690 - -0.2 180,690 - +2.0 180,701 - -0.8 180,793 - EX31_RS00830 0.18 +1.2 180,802 - EX31_RS00830 0.20 +0.9 180,829 - EX31_RS00830 0.23 -1.1 180,874 + EX31_RS00830 0.29 +2.5 180,875 - EX31_RS00830 0.29 +1.0 180,875 - EX31_RS00830 0.29 +1.5
Or see this region's nucleotide sequence