Strain Fitness in Pedobacter sp. GW460-11-11-14-LB5 around CA265_RS23435

Experiment: R2A with Spectinomycin 0.025 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Spectinomycin 0.025 mg/ml
5,730,249	+				-0.1
5,730,255	+				+0.8
5,730,255	+				+0.6
5,730,302	-				+0.2
5,730,302	-				-1.3
5,730,305	+				+0.1
5,730,315	+				+0.7
5,730,334	+				+1.2
5,730,337	+				+1.4
5,730,355	+				-0.4
5,730,388	+		CA265_RS23430	0.13	-0.4
5,730,388	+		CA265_RS23430	0.13	+0.3
5,730,441	-		CA265_RS23430	0.22	-0.6
5,730,481	+		CA265_RS23430	0.29	-0.7
5,730,481	+		CA265_RS23430	0.29	+0.2
5,730,495	-		CA265_RS23430	0.32	+0.6
5,730,497	-		CA265_RS23430	0.32	-1.2
5,730,497	-		CA265_RS23430	0.32	-0.1
5,730,535	-		CA265_RS23430	0.39	-0.4
5,730,571	-		CA265_RS23430	0.45	-1.0
5,730,589	-		CA265_RS23430	0.48	-0.2
5,730,615	+		CA265_RS23430	0.53	+0.3
5,730,676	+		CA265_RS23430	0.63	+0.4
5,730,676	+		CA265_RS23430	0.63	-0.4
5,730,677	-		CA265_RS23430	0.64	-0.8
5,730,677	-		CA265_RS23430	0.64	-0.3
5,730,776	-		CA265_RS23430	0.81	+1.4
5,730,778	+		CA265_RS23430	0.81	-0.2
5,730,778	+		CA265_RS23430	0.81	-1.8
5,730,902	+				+0.2
5,730,984	-				-1.5
5,731,009	+		CA265_RS23435	0.12	-0.6
5,731,027	+		CA265_RS23435	0.14	-1.4
5,731,028	-		CA265_RS23435	0.14	-1.0
5,731,096	-		CA265_RS23435	0.23	-0.3
5,731,097	+		CA265_RS23435	0.23	-0.0
5,731,147	+		CA265_RS23435	0.30	-0.8
5,731,148	-		CA265_RS23435	0.30	-0.9
5,731,165	+		CA265_RS23435	0.32	-1.1
5,731,169	-		CA265_RS23435	0.33	-1.0
5,731,214	-		CA265_RS23435	0.39	+0.2
5,731,233	+		CA265_RS23435	0.41	-1.3
5,731,234	-		CA265_RS23435	0.41	-1.1
5,731,238	+		CA265_RS23435	0.42	-1.2
5,731,239	-		CA265_RS23435	0.42	-0.8
5,731,239	-		CA265_RS23435	0.42	-1.1
5,731,241	-		CA265_RS23435	0.42	-2.1
5,731,253	-		CA265_RS23435	0.44	-0.8
5,731,273	+		CA265_RS23435	0.47	-2.3
5,731,273	+		CA265_RS23435	0.47	-1.6
5,731,325	-		CA265_RS23435	0.53	-1.9
5,731,351	-		CA265_RS23435	0.57	-0.3
5,731,352	+		CA265_RS23435	0.57	-1.4
5,731,353	-		CA265_RS23435	0.57	-0.4
5,731,353	-		CA265_RS23435	0.57	-0.8
5,731,353	-		CA265_RS23435	0.57	-0.5
5,731,373	+		CA265_RS23435	0.60	-0.3
5,731,386	-		CA265_RS23435	0.62	-0.4
5,731,390	+		CA265_RS23435	0.62	-0.1
5,731,390	+		CA265_RS23435	0.62	-0.3
5,731,402	+		CA265_RS23435	0.64	-1.4
5,731,445	+		CA265_RS23435	0.69	-0.5
5,731,461	+		CA265_RS23435	0.71	-1.1
5,731,501	+		CA265_RS23435	0.77	-2.2
5,731,502	-		CA265_RS23435	0.77	-0.4
5,731,574	-		CA265_RS23435	0.86	+2.7
5,731,592	-		CA265_RS23435	0.89	-1.2
5,731,617	+				+1.3
5,731,618	-				-1.6
5,731,675	-				-1.0
5,731,685	-				-0.5
5,731,695	+				+0.0
5,731,695	+				+0.0
5,731,797	+				-1.9
5,731,852	-				+0.5
5,731,893	-				-1.6
5,731,898	+				-1.9
5,731,930	+				-1.0
5,731,940	-				+0.3
5,731,946	+				-1.8
5,731,949	+				+0.3
5,731,949	+				-0.7
5,731,950	-				-0.5
5,731,955	-				-0.5
5,731,969	-				+1.4
5,732,025	-				-0.7
5,732,027	-				+0.8
5,732,027	-				+0.6
5,732,027	-				+0.3
5,732,027	-				+0.0
5,732,076	-				+0.2
5,732,091	+				-0.1
5,732,093	+				-0.3
5,732,094	-				+0.0
5,732,094	-				+0.0
5,732,149	+				+0.4
5,732,195	+				-0.3
5,732,196	-				+1.9
5,732,204	-				+0.3
5,732,214	+				-0.3
5,732,255	+				-0.8
5,732,256	-				+0.4
5,732,259	-				+0.1
5,732,378	+				+0.5
5,732,399	+		CA265_RS23440	0.10	+0.5
5,732,400	-		CA265_RS23440	0.10	+0.6
5,732,440	+		CA265_RS23440	0.11	+0.5
5,732,490	+		CA265_RS23440	0.13	-0.5
5,732,533	+		CA265_RS23440	0.14	-3.2
5,732,534	-		CA265_RS23440	0.14	+0.3
5,732,534	-		CA265_RS23440	0.14	+0.7
5,732,537	-		CA265_RS23440	0.14	-1.0
5,732,537	-		CA265_RS23440	0.14	-0.2
5,732,564	-		CA265_RS23440	0.15	+0.1
5,732,564	-		CA265_RS23440	0.15	-0.4
5,732,608	+		CA265_RS23440	0.16	-1.5
5,732,609	-		CA265_RS23440	0.16	+0.3