Strain Fitness in Pedobacter sp. GW460-11-11-14-LB5 around CA265_RS22770

Experiment: R2A with Spectinomycin 0.025 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Spectinomycin 0.025 mg/ml
5,570,652	+		CA265_RS22765	0.25	-0.2
5,570,652	+		CA265_RS22765	0.25	-0.2
5,570,707	-		CA265_RS22765	0.30	+0.5
5,570,707	-		CA265_RS22765	0.30	+0.0
5,570,710	+		CA265_RS22765	0.31	+1.2
5,570,711	-		CA265_RS22765	0.31	-0.2
5,570,711	-		CA265_RS22765	0.31	-0.3
5,570,717	-		CA265_RS22765	0.31	+0.2
5,570,777	-		CA265_RS22765	0.37	-0.3
5,570,791	-		CA265_RS22765	0.39	-0.1
5,570,886	+		CA265_RS22765	0.48	-1.4
5,570,887	-		CA265_RS22765	0.48	-0.4
5,570,887	-		CA265_RS22765	0.48	+0.1
5,570,908	+		CA265_RS22765	0.50	+1.8
5,570,916	+		CA265_RS22765	0.51	+0.4
5,570,931	+		CA265_RS22765	0.53	-0.1
5,570,973	+		CA265_RS22765	0.57	-0.9
5,570,993	-		CA265_RS22765	0.59	-0.8
5,570,993	-		CA265_RS22765	0.59	-0.2
5,570,993	-		CA265_RS22765	0.59	-1.0
5,571,046	-		CA265_RS22765	0.64	+0.3
5,571,095	+		CA265_RS22765	0.69	-0.2
5,571,127	-		CA265_RS22765	0.72	+0.0
5,571,127	-		CA265_RS22765	0.72	+0.0
5,571,139	+		CA265_RS22765	0.73	+0.1
5,571,150	+		CA265_RS22765	0.75	-0.2
5,571,151	-		CA265_RS22765	0.75	+0.8
5,571,173	-		CA265_RS22765	0.77	-0.8
5,571,189	+		CA265_RS22765	0.78	-1.0
5,571,191	+		CA265_RS22765	0.79	-0.1
5,571,331	-				-0.3
5,571,334	-				-0.1
5,571,438	+				+0.2
5,571,454	+				-1.1
5,571,454	+				+0.4
5,571,456	+				+0.1
5,571,477	-				-0.2
5,571,488	+				-0.5
5,571,500	-				+0.9
5,571,514	-				+0.7
5,571,591	-				-1.5
5,571,593	+				+0.2
5,571,626	-				-0.8
5,571,634	+				-2.3
5,571,687	+				-1.0
5,571,688	-				-0.6
5,571,714	+				+1.1
5,571,780	-		CA265_RS22770	0.15	-0.3
5,571,835	+		CA265_RS22770	0.20	-2.4
5,571,849	+		CA265_RS22770	0.22	-0.3
5,571,861	+		CA265_RS22770	0.23	-2.8
5,571,903	+		CA265_RS22770	0.27	+0.4
5,571,904	-		CA265_RS22770	0.27	-2.7
5,571,916	-		CA265_RS22770	0.28	-0.6
5,571,916	-		CA265_RS22770	0.28	-0.6
5,571,918	+		CA265_RS22770	0.28	+0.4
5,571,940	+		CA265_RS22770	0.30	-1.5
5,571,993	-		CA265_RS22770	0.35	-0.5
5,571,993	-		CA265_RS22770	0.35	-1.3
5,571,993	-		CA265_RS22770	0.35	-1.1
5,571,994	+		CA265_RS22770	0.35	-0.3
5,572,007	+		CA265_RS22770	0.36	-1.3
5,572,075	+				-0.2
5,572,114	-		CA265_RS22770	0.46	-1.9
5,572,115	+		CA265_RS22770	0.46	-0.4
5,572,136	-		CA265_RS22770	0.48	-0.4
5,572,316	-		CA265_RS22770	0.64	-0.9
5,572,326	-		CA265_RS22770	0.65	-0.5
5,572,404	-		CA265_RS22770	0.72	-0.6
5,572,409	+		CA265_RS22770	0.72	-1.4
5,572,409	+		CA265_RS22770	0.72	-0.9
5,572,409	+		CA265_RS22770	0.72	-2.6
5,572,410	-		CA265_RS22770	0.72	-0.9
5,572,437	+		CA265_RS22770	0.75	-1.8
5,572,555	-		CA265_RS22770	0.85	-0.3
5,572,561	-		CA265_RS22770	0.86	-0.9
5,572,627	+				-0.3
5,572,628	-				-0.8
5,572,632	+				-0.3
5,572,633	-				-2.0
5,572,633	-				-0.9
5,572,669	-				-0.6
5,572,711	-				-1.3
5,572,745	-				-0.6
5,572,767	+				+0.0
5,572,767	+				+0.6
5,572,768	-				+0.1
5,572,770	+				+0.8
5,572,877	+				-0.6
5,572,877	+				-1.6
5,572,911	+		CA265_RS22775	0.13	-1.1
5,572,967	+		CA265_RS22775	0.18	-1.9
5,573,092	+		CA265_RS22775	0.29	-1.0
5,573,103	-		CA265_RS22775	0.30	-2.1
5,573,176	-		CA265_RS22775	0.36	-1.2
5,573,239	+		CA265_RS22775	0.42	-1.1
5,573,239	+		CA265_RS22775	0.42	+0.7
5,573,239	+		CA265_RS22775	0.42	-1.2
5,573,240	-		CA265_RS22775	0.42	-0.8
5,573,280	+		CA265_RS22775	0.46	-1.3
5,573,280	+		CA265_RS22775	0.46	+0.2
5,573,281	-		CA265_RS22775	0.46	-0.9
5,573,306	-		CA265_RS22775	0.48	-0.9
5,573,364	+		CA265_RS22775	0.53	-1.9
5,573,370	-		CA265_RS22775	0.54	+0.4
5,573,396	+		CA265_RS22775	0.56	-0.6
5,573,401	-		CA265_RS22775	0.57	-1.5
5,573,473	+		CA265_RS22775	0.63	-0.2
5,573,492	+		CA265_RS22775	0.65	-0.6
5,573,557	+		CA265_RS22775	0.71	-0.3
5,573,609	-		CA265_RS22775	0.75	-1.9
5,573,616	+		CA265_RS22775	0.76	-0.3
5,573,623	-		CA265_RS22775	0.77	-2.1
5,573,638	-		CA265_RS22775	0.78	-1.0
5,573,677	+		CA265_RS22775	0.81	-1.6
5,573,680	+		CA265_RS22775	0.82	-0.5
5,573,681	-		CA265_RS22775	0.82	-0.1
5,573,681	-		CA265_RS22775	0.82	-0.8
5,573,681	-		CA265_RS22775	0.82	-1.2
5,573,684	+		CA265_RS22775	0.82	-0.4