Experiment: R2A with Spectinomycin 0.025 mg/ml 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
    
        
         
    
        
         
    
        
         
    
         
     
500 nt CA265_RS21710 and CA265_RS21715 are separated by 345 nucleotides CA265_RS21715 and CA265_RS21720 overlap by 8 nucleotides CA265_RS21720 and CA265_RS21725 are separated by 216 nucleotides 
        CA265_RS21710: CA265_RS21710 - anion permease, at 5,339,495 to 5,340,505 
        _RS21710 
         
        
        CA265_RS21715: CA265_RS21715 - hypothetical protein, at 5,340,851 to 5,342,158 
        _RS21715 
         
        
        CA265_RS21720: CA265_RS21720 - RNA polymerase subunit sigma-70, at 5,342,151 to 5,342,729 
        _RS21720 
         
        
        CA265_RS21725: CA265_RS21725 - hypothetical protein, at 5,342,946 to 5,343,707 
        _RS21725 
         Position (kb) 5340 
5341 
5342 
5343 Strain fitness (log2 ratio) -2 
-1 
0 
1 
2 at 5340.522 kb on + strand at 5340.525 kb on - strand at 5340.644 kb on - strand at 5340.644 kb on - strand at 5340.650 kb on - strand at 5340.650 kb on - strand at 5340.662 kb on - strand at 5340.707 kb on + strand at 5340.708 kb on - strand at 5340.708 kb on - strand at 5340.796 kb on - strand at 5340.799 kb on + strand at 5340.835 kb on - strand at 5340.835 kb on - strand at 5340.837 kb on + strand at 5340.838 kb on - strand at 5340.838 kb on - strand at 5340.859 kb on + strand at 5340.974 kb on - strand at 5341.211 kb on + strand, within CA265_RS21715 at 5341.244 kb on + strand, within CA265_RS21715 at 5341.256 kb on - strand, within CA265_RS21715 at 5341.283 kb on + strand, within CA265_RS21715 at 5341.312 kb on + strand, within CA265_RS21715 at 5341.356 kb on + strand, within CA265_RS21715 at 5341.410 kb on - strand, within CA265_RS21715 at 5341.424 kb on + strand, within CA265_RS21715 at 5341.484 kb on + strand, within CA265_RS21715 at 5341.523 kb on + strand at 5341.524 kb on - strand, within CA265_RS21715 at 5341.550 kb on - strand, within CA265_RS21715 at 5341.551 kb on + strand, within CA265_RS21715 at 5341.551 kb on + strand, within CA265_RS21715 at 5341.552 kb on - strand, within CA265_RS21715 at 5341.552 kb on - strand, within CA265_RS21715 at 5341.647 kb on - strand, within CA265_RS21715 at 5341.716 kb on - strand, within CA265_RS21715 at 5341.716 kb on - strand, within CA265_RS21715 at 5341.747 kb on + strand, within CA265_RS21715 at 5341.786 kb on + strand, within CA265_RS21715 at 5341.803 kb on - strand, within CA265_RS21715 at 5341.913 kb on + strand, within CA265_RS21715 at 5341.913 kb on + strand, within CA265_RS21715 at 5341.914 kb on - strand, within CA265_RS21715 at 5341.975 kb on - strand, within CA265_RS21715 at 5341.993 kb on + strand, within CA265_RS21715 at 5342.175 kb on - strand at 5342.209 kb on + strand, within CA265_RS21720 at 5342.222 kb on + strand, within CA265_RS21720 at 5342.223 kb on - strand, within CA265_RS21720 at 5342.223 kb on - strand, within CA265_RS21720 at 5342.229 kb on - strand, within CA265_RS21720 at 5342.274 kb on + strand, within CA265_RS21720 at 5342.289 kb on + strand, within CA265_RS21720 at 5342.290 kb on - strand, within CA265_RS21720 at 5342.325 kb on - strand, within CA265_RS21720 at 5342.343 kb on - strand, within CA265_RS21720 at 5342.367 kb on + strand, within CA265_RS21720 at 5342.368 kb on - strand, within CA265_RS21720 at 5342.412 kb on + strand, within CA265_RS21720 at 5342.412 kb on + strand, within CA265_RS21720 at 5342.509 kb on + strand, within CA265_RS21720 at 5342.615 kb on + strand, within CA265_RS21720 at 5342.615 kb on + strand, within CA265_RS21720 at 5342.617 kb on + strand, within CA265_RS21720 at 5342.618 kb on - strand, within CA265_RS21720 at 5342.618 kb on - strand, within CA265_RS21720 at 5342.621 kb on + strand, within CA265_RS21720 at 5342.627 kb on - strand, within CA265_RS21720 at 5342.720 kb on + strand at 5342.742 kb on - strand at 5342.764 kb on + strand at 5342.791 kb on + strand at 5342.857 kb on + strand at 5342.885 kb on - strand at 5342.885 kb on - strand at 5342.885 kb on - strand at 5342.897 kb on - strand at 5342.924 kb on + strand at 5342.961 kb on - strand at 5342.966 kb on - strand at 5343.002 kb on - strand at 5343.002 kb on - strand at 5343.033 kb on - strand, within CA265_RS21725 at 5343.034 kb on + strand, within CA265_RS21725 at 5343.034 kb on + strand, within CA265_RS21725  
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction R2A with Spectinomycin 0.025 mg/ml remove 5,340,522 +  +1.1 5,340,525 -  +0.8 5,340,644 -  -0.7 5,340,644 -  +1.1 5,340,650 -  -0.6 5,340,650 -  -0.1 5,340,662 -  -0.3 5,340,707 +  +0.6 5,340,708 -  -0.2 5,340,708 -  -1.7 5,340,796 -  -0.3 5,340,799 +  -0.2 5,340,835 -  +1.4 5,340,835 -  +0.1 5,340,837 +  +0.2 5,340,838 -  +0.8 5,340,838 -  -0.2 5,340,859 +  -0.8 5,340,974 -  -1.2 5,341,211 +  CA265_RS21715 0.28  -2.6 5,341,244 +  CA265_RS21715 0.30  -1.1 5,341,256 -  CA265_RS21715 0.31  -1.1 5,341,283 +  CA265_RS21715 0.33  -0.3 5,341,312 +  CA265_RS21715 0.35  -0.6 5,341,356 +  CA265_RS21715 0.39  +1.9 5,341,410 -  CA265_RS21715 0.43  -0.1 5,341,424 +  CA265_RS21715 0.44  -1.8 5,341,484 +  CA265_RS21715 0.48  -1.1 5,341,523 +  -0.7 5,341,524 -  CA265_RS21715 0.51  -0.0 5,341,550 -  CA265_RS21715 0.53  -0.8 5,341,551 +  CA265_RS21715 0.54  -0.5 5,341,551 +  CA265_RS21715 0.54  +0.3 5,341,552 -  CA265_RS21715 0.54  -2.0 5,341,552 -  CA265_RS21715 0.54  +0.5 5,341,647 -  CA265_RS21715 0.61  -0.7 5,341,716 -  CA265_RS21715 0.66  -0.5 5,341,716 -  CA265_RS21715 0.66  -0.1 5,341,747 +  CA265_RS21715 0.69  -1.2 5,341,786 +  CA265_RS21715 0.71  -0.3 5,341,803 -  CA265_RS21715 0.73  -1.0 5,341,913 +  CA265_RS21715 0.81  +0.2 5,341,913 +  CA265_RS21715 0.81  +0.3 5,341,914 -  CA265_RS21715 0.81  -0.3 5,341,975 -  CA265_RS21715 0.86  +0.6 5,341,993 +  CA265_RS21715 0.87  -0.1 5,342,175 -  -0.2 5,342,209 +  CA265_RS21720 0.10  -0.1 5,342,222 +  CA265_RS21720 0.12  -0.1 5,342,223 -  CA265_RS21720 0.12  +1.5 5,342,223 -  CA265_RS21720 0.12  +1.3 5,342,229 -  CA265_RS21720 0.13  -0.8 5,342,274 +  CA265_RS21720 0.21  -1.4 5,342,289 +  CA265_RS21720 0.24  -0.0 5,342,290 -  CA265_RS21720 0.24  +0.0 5,342,325 -  CA265_RS21720 0.30  +0.7 5,342,343 -  CA265_RS21720 0.33  +0.1 5,342,367 +  CA265_RS21720 0.37  +0.7 5,342,368 -  CA265_RS21720 0.37  +0.3 5,342,412 +  CA265_RS21720 0.45  -0.7 5,342,412 +  CA265_RS21720 0.45  +0.2 5,342,509 +  CA265_RS21720 0.62  -0.7 5,342,615 +  CA265_RS21720 0.80  -0.5 5,342,615 +  CA265_RS21720 0.80  +1.6 5,342,617 +  CA265_RS21720 0.80  +0.0 5,342,618 -  CA265_RS21720 0.81  +0.0 5,342,618 -  CA265_RS21720 0.81  +0.1 5,342,621 +  CA265_RS21720 0.81  -0.1 5,342,627 -  CA265_RS21720 0.82  +1.2 5,342,720 +  +0.0 5,342,742 -  +1.1 5,342,764 +  -0.6 5,342,791 +  -0.7 5,342,857 +  -0.5 5,342,885 -  -1.0 5,342,885 -  -1.3 5,342,885 -  +0.2 5,342,897 -  -0.2 5,342,924 +  +0.5 5,342,961 -  +1.0 5,342,966 -  +0.0 5,343,002 -  -0.7 5,343,002 -  +1.0 5,343,033 -  CA265_RS21725 0.11  -0.2 5,343,034 +  CA265_RS21725 0.12  -0.4 5,343,034 +  CA265_RS21725 0.12  +0.8 
Or see this region's nucleotide sequence