Experiment: R2A with Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS20580 and CA265_RS20585 are separated by 22 nucleotides CA265_RS20585 and CA265_RS20590 overlap by 4 nucleotides
CA265_RS20580: CA265_RS20580 - hypothetical protein, at 5,090,435 to 5,093,392
_RS20580
CA265_RS20585: CA265_RS20585 - hypothetical protein, at 5,093,415 to 5,093,645
_RS20585
CA265_RS20590: CA265_RS20590 - hypothetical protein, at 5,093,642 to 5,095,966
_RS20590
Position (kb)
5093
5094 Strain fitness (log2 ratio)
-1
0
1
2 at 5092.427 kb on + strand, within CA265_RS20580 at 5092.431 kb on + strand, within CA265_RS20580 at 5092.433 kb on + strand, within CA265_RS20580 at 5092.460 kb on + strand, within CA265_RS20580 at 5092.460 kb on + strand, within CA265_RS20580 at 5092.460 kb on + strand, within CA265_RS20580 at 5092.460 kb on + strand, within CA265_RS20580 at 5092.480 kb on + strand, within CA265_RS20580 at 5092.493 kb on + strand, within CA265_RS20580 at 5092.530 kb on + strand, within CA265_RS20580 at 5092.530 kb on + strand at 5092.576 kb on + strand, within CA265_RS20580 at 5092.576 kb on + strand, within CA265_RS20580 at 5092.633 kb on + strand, within CA265_RS20580 at 5092.633 kb on + strand, within CA265_RS20580 at 5092.709 kb on + strand, within CA265_RS20580 at 5092.714 kb on + strand, within CA265_RS20580 at 5092.718 kb on + strand, within CA265_RS20580 at 5092.718 kb on + strand, within CA265_RS20580 at 5093.074 kb on + strand at 5093.122 kb on + strand at 5093.122 kb on + strand at 5093.122 kb on + strand at 5093.177 kb on + strand at 5093.208 kb on + strand at 5093.269 kb on + strand at 5093.269 kb on + strand at 5093.275 kb on + strand at 5093.444 kb on + strand, within CA265_RS20585 at 5093.506 kb on + strand, within CA265_RS20585 at 5093.531 kb on + strand, within CA265_RS20585 at 5093.648 kb on + strand at 5093.732 kb on + strand at 5093.732 kb on + strand at 5093.797 kb on + strand at 5093.799 kb on + strand at 5093.799 kb on + strand at 5093.799 kb on + strand at 5094.028 kb on + strand, within CA265_RS20590 at 5094.214 kb on + strand, within CA265_RS20590 at 5094.231 kb on + strand, within CA265_RS20590 at 5094.231 kb on + strand, within CA265_RS20590 at 5094.265 kb on + strand, within CA265_RS20590 at 5094.265 kb on + strand, within CA265_RS20590 at 5094.265 kb on + strand, within CA265_RS20590 at 5094.480 kb on + strand, within CA265_RS20590 at 5094.537 kb on + strand, within CA265_RS20590 at 5094.539 kb on + strand, within CA265_RS20590
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Spectinomycin 0.025 mg/ml remove 5,092,427 + CA265_RS20580 0.67 -0.1 5,092,431 + CA265_RS20580 0.67 -0.3 5,092,433 + CA265_RS20580 0.68 -0.4 5,092,460 + CA265_RS20580 0.68 -0.1 5,092,460 + CA265_RS20580 0.68 -0.2 5,092,460 + CA265_RS20580 0.68 +0.1 5,092,460 + CA265_RS20580 0.68 +0.8 5,092,480 + CA265_RS20580 0.69 -0.3 5,092,493 + CA265_RS20580 0.70 +0.3 5,092,530 + CA265_RS20580 0.71 -0.5 5,092,530 + -0.4 5,092,576 + CA265_RS20580 0.72 +0.3 5,092,576 + CA265_RS20580 0.72 +0.8 5,092,633 + CA265_RS20580 0.74 -0.0 5,092,633 + CA265_RS20580 0.74 +0.4 5,092,709 + CA265_RS20580 0.77 +0.3 5,092,714 + CA265_RS20580 0.77 +0.2 5,092,718 + CA265_RS20580 0.77 +0.4 5,092,718 + CA265_RS20580 0.77 +0.1 5,093,074 + -0.1 5,093,122 + +0.6 5,093,122 + -0.5 5,093,122 + +1.1 5,093,177 + +0.3 5,093,208 + +0.4 5,093,269 + +0.7 5,093,269 + +0.6 5,093,275 + -0.1 5,093,444 + CA265_RS20585 0.13 -1.7 5,093,506 + CA265_RS20585 0.39 +0.2 5,093,531 + CA265_RS20585 0.50 -1.4 5,093,648 + -0.5 5,093,732 + -0.2 5,093,732 + -1.4 5,093,797 + -0.9 5,093,799 + -0.9 5,093,799 + +1.5 5,093,799 + +0.7 5,094,028 + CA265_RS20590 0.17 -0.1 5,094,214 + CA265_RS20590 0.25 +0.6 5,094,231 + CA265_RS20590 0.25 -0.3 5,094,231 + CA265_RS20590 0.25 -0.6 5,094,265 + CA265_RS20590 0.27 -0.5 5,094,265 + CA265_RS20590 0.27 +0.7 5,094,265 + CA265_RS20590 0.27 +1.5 5,094,480 + CA265_RS20590 0.36 +0.2 5,094,537 + CA265_RS20590 0.38 -0.9 5,094,539 + CA265_RS20590 0.39 +1.9
Or see this region's nucleotide sequence