Experiment: R2A with Spectinomycin 0.025 mg/ml 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
    
        
         
    
        
         
    
        
         
    
         
     
500 nt CA265_RS19215 and CA265_RS19220 are separated by 61 nucleotides CA265_RS19220 and CA265_RS19225 are separated by 15 nucleotides CA265_RS19225 and CA265_RS19230 overlap by 4 nucleotides 
        CA265_RS19215: CA265_RS19215 - nucleoside-diphosphate-sugar epimerase, at 4,776,120 to 4,777,031 
        _RS19215 
         
        
        CA265_RS19220: CA265_RS19220 - hypothetical protein, at 4,777,093 to 4,777,842 
        _RS19220 
         
        
        CA265_RS19225: CA265_RS19225 - hypothetical protein, at 4,777,858 to 4,778,862 
        _RS19225 
         
        
        CA265_RS19230: CA265_RS19230 - hypothetical protein, at 4,778,859 to 4,779,971 
        _RS19230 
         Position (kb) 4777 
4778 
4779 Strain fitness (log2 ratio) -3 
-2 
-1 
0 
1 
2 
3 at 4776.928 kb on - strand, within CA265_RS19215 at 4776.928 kb on - strand, within CA265_RS19215 at 4776.974 kb on - strand at 4776.974 kb on - strand at 4776.978 kb on - strand at 4777.021 kb on - strand at 4777.157 kb on + strand at 4777.214 kb on - strand, within CA265_RS19220 at 4777.332 kb on + strand, within CA265_RS19220 at 4777.387 kb on + strand, within CA265_RS19220 at 4777.415 kb on + strand, within CA265_RS19220 at 4777.453 kb on + strand, within CA265_RS19220 at 4777.597 kb on + strand, within CA265_RS19220 at 4777.609 kb on - strand, within CA265_RS19220 at 4777.710 kb on + strand, within CA265_RS19220 at 4777.711 kb on - strand, within CA265_RS19220 at 4777.739 kb on + strand, within CA265_RS19220 at 4777.740 kb on - strand, within CA265_RS19220 at 4777.846 kb on - strand at 4777.859 kb on + strand at 4777.874 kb on + strand at 4777.914 kb on + strand at 4777.948 kb on - strand at 4777.951 kb on + strand at 4777.996 kb on - strand, within CA265_RS19225 at 4778.005 kb on - strand, within CA265_RS19225 at 4778.069 kb on + strand, within CA265_RS19225 at 4778.180 kb on + strand, within CA265_RS19225 at 4778.180 kb on + strand, within CA265_RS19225 at 4778.181 kb on - strand, within CA265_RS19225 at 4778.183 kb on + strand, within CA265_RS19225 at 4778.184 kb on - strand, within CA265_RS19225 at 4778.184 kb on - strand, within CA265_RS19225 at 4778.228 kb on + strand, within CA265_RS19225 at 4778.239 kb on + strand, within CA265_RS19225 at 4778.256 kb on - strand, within CA265_RS19225 at 4778.312 kb on - strand, within CA265_RS19225 at 4778.374 kb on + strand, within CA265_RS19225 at 4778.377 kb on + strand, within CA265_RS19225 at 4778.397 kb on + strand, within CA265_RS19225 at 4778.444 kb on + strand, within CA265_RS19225 at 4778.452 kb on - strand, within CA265_RS19225 at 4778.510 kb on + strand, within CA265_RS19225 at 4778.539 kb on - strand, within CA265_RS19225 at 4778.561 kb on - strand, within CA265_RS19225 at 4778.601 kb on - strand, within CA265_RS19225 at 4778.707 kb on - strand, within CA265_RS19225 at 4778.731 kb on - strand, within CA265_RS19225 at 4778.734 kb on + strand, within CA265_RS19225 at 4778.821 kb on - strand at 4778.915 kb on + strand at 4778.915 kb on + strand at 4779.012 kb on - strand, within CA265_RS19230 at 4779.012 kb on - strand, within CA265_RS19230 at 4779.048 kb on + strand, within CA265_RS19230 at 4779.051 kb on + strand, within CA265_RS19230 at 4779.051 kb on + strand, within CA265_RS19230 at 4779.057 kb on - strand, within CA265_RS19230 at 4779.111 kb on + strand, within CA265_RS19230 at 4779.201 kb on + strand, within CA265_RS19230 at 4779.273 kb on - strand, within CA265_RS19230 at 4779.353 kb on + strand, within CA265_RS19230 at 4779.395 kb on - strand, within CA265_RS19230 at 4779.399 kb on + strand, within CA265_RS19230 at 4779.399 kb on + strand, within CA265_RS19230 at 4779.443 kb on - strand, within CA265_RS19230 at 4779.470 kb on + strand, within CA265_RS19230 at 4779.471 kb on - strand, within CA265_RS19230 at 4779.474 kb on + strand, within CA265_RS19230 at 4779.530 kb on + strand, within CA265_RS19230 at 4779.569 kb on + strand, within CA265_RS19230 at 4779.727 kb on + strand, within CA265_RS19230 at 4779.743 kb on + strand, within CA265_RS19230 at 4779.758 kb on - strand, within CA265_RS19230 at 4779.771 kb on + strand, within CA265_RS19230 at 4779.773 kb on + strand, within CA265_RS19230 at 4779.773 kb on + strand, within CA265_RS19230 at 4779.773 kb on + strand, within CA265_RS19230 at 4779.805 kb on - strand, within CA265_RS19230 at 4779.852 kb on + strand, within CA265_RS19230 at 4779.853 kb on - strand, within CA265_RS19230 at 4779.861 kb on + strand  
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction R2A with Spectinomycin 0.025 mg/ml remove 4,776,928 -  CA265_RS19215 0.89  -1.3 4,776,928 -  CA265_RS19215 0.89  -3.4 4,776,974 -  -1.3 4,776,974 -  -0.2 4,776,978 -  -2.8 4,777,021 -  -2.3 4,777,157 +  -1.3 4,777,214 -  CA265_RS19220 0.16  -0.7 4,777,332 +  CA265_RS19220 0.32  -0.9 4,777,387 +  CA265_RS19220 0.39  -1.9 4,777,415 +  CA265_RS19220 0.43  +0.1 4,777,453 +  CA265_RS19220 0.48  -0.4 4,777,597 +  CA265_RS19220 0.67  -1.6 4,777,609 -  CA265_RS19220 0.69  -1.5 4,777,710 +  CA265_RS19220 0.82  -0.3 4,777,711 -  CA265_RS19220 0.82  -2.7 4,777,739 +  CA265_RS19220 0.86  -1.5 4,777,740 -  CA265_RS19220 0.86  -0.8 4,777,846 -  -0.7 4,777,859 +  -1.6 4,777,874 +  -2.7 4,777,914 +  -2.2 4,777,948 -  -0.5 4,777,951 +  -2.2 4,777,996 -  CA265_RS19225 0.14  -1.2 4,778,005 -  CA265_RS19225 0.15  +0.8 4,778,069 +  CA265_RS19225 0.21  -1.3 4,778,180 +  CA265_RS19225 0.32  -0.4 4,778,180 +  CA265_RS19225 0.32  -2.5 4,778,181 -  CA265_RS19225 0.32  -1.3 4,778,183 +  CA265_RS19225 0.32  -3.1 4,778,184 -  CA265_RS19225 0.32  -1.2 4,778,184 -  CA265_RS19225 0.32  -2.6 4,778,228 +  CA265_RS19225 0.37  +0.8 4,778,239 +  CA265_RS19225 0.38  -0.7 4,778,256 -  CA265_RS19225 0.40  -3.1 4,778,312 -  CA265_RS19225 0.45  -2.2 4,778,374 +  CA265_RS19225 0.51  -1.0 4,778,377 +  CA265_RS19225 0.52  -1.9 4,778,397 +  CA265_RS19225 0.54  -1.2 4,778,444 +  CA265_RS19225 0.58  -1.6 4,778,452 -  CA265_RS19225 0.59  +1.4 4,778,510 +  CA265_RS19225 0.65  -2.0 4,778,539 -  CA265_RS19225 0.68  -1.9 4,778,561 -  CA265_RS19225 0.70  -1.3 4,778,601 -  CA265_RS19225 0.74  -1.2 4,778,707 -  CA265_RS19225 0.84  +3.2 4,778,731 -  CA265_RS19225 0.87  -0.2 4,778,734 +  CA265_RS19225 0.87  -1.2 4,778,821 -  -3.6 4,778,915 +  -1.2 4,778,915 +  -3.5 4,779,012 -  CA265_RS19230 0.14  -0.3 4,779,012 -  CA265_RS19230 0.14  -1.9 4,779,048 +  CA265_RS19230 0.17  -1.5 4,779,051 +  CA265_RS19230 0.17  -2.1 4,779,051 +  CA265_RS19230 0.17  -1.4 4,779,057 -  CA265_RS19230 0.18  -1.9 4,779,111 +  CA265_RS19230 0.23  -2.3 4,779,201 +  CA265_RS19230 0.31  +0.7 4,779,273 -  CA265_RS19230 0.37  -2.0 4,779,353 +  CA265_RS19230 0.44  -1.7 4,779,395 -  CA265_RS19230 0.48  -1.5 4,779,399 +  CA265_RS19230 0.49  -0.2 4,779,399 +  CA265_RS19230 0.49  -0.7 4,779,443 -  CA265_RS19230 0.52  -1.2 4,779,470 +  CA265_RS19230 0.55  -1.4 4,779,471 -  CA265_RS19230 0.55  -0.7 4,779,474 +  CA265_RS19230 0.55  -0.7 4,779,530 +  CA265_RS19230 0.60  -1.3 4,779,569 +  CA265_RS19230 0.64  -1.8 4,779,727 +  CA265_RS19230 0.78  +0.3 4,779,743 +  CA265_RS19230 0.79  -1.0 4,779,758 -  CA265_RS19230 0.81  -1.6 4,779,771 +  CA265_RS19230 0.82  -0.2 4,779,773 +  CA265_RS19230 0.82  -0.2 4,779,773 +  CA265_RS19230 0.82  -2.2 4,779,773 +  CA265_RS19230 0.82  -1.9 4,779,805 -  CA265_RS19230 0.85  -2.4 4,779,852 +  CA265_RS19230 0.89  -1.8 4,779,853 -  CA265_RS19230 0.89  -3.2 4,779,861 +  -3.4 
Or see this region's nucleotide sequence