Experiment: R2A with Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS18840 and CA265_RS18845 are separated by 2 nucleotides CA265_RS18845 and CA265_RS18850 are separated by 29 nucleotides CA265_RS18850 and CA265_RS18855 overlap by 1 nucleotides CA265_RS18855 and CA265_RS18860 are separated by 337 nucleotides
CA265_RS18840: CA265_RS18840 - transposase, at 4,687,457 to 4,687,897
_RS18840
CA265_RS18845: CA265_RS18845 - GNAT family N-acetyltransferase, at 4,687,900 to 4,688,457
_RS18845
CA265_RS18850: CA265_RS18850 - hypothetical protein, at 4,688,487 to 4,688,687
_RS18850
CA265_RS18855: CA265_RS18855 - hypothetical protein, at 4,688,687 to 4,689,136
_RS18855
CA265_RS18860: CA265_RS18860 - alpha-ketoacid dehydrogenase subunit beta, at 4,689,474 to 4,690,460
_RS18860
Position (kb) 4688
4689 Strain fitness (log2 ratio) -1
0
1
2
3 at 4687.515 kb on - strand, within CA265_RS18840 at 4687.674 kb on + strand, within CA265_RS18840 at 4687.675 kb on - strand, within CA265_RS18840 at 4687.708 kb on + strand, within CA265_RS18840 at 4687.723 kb on - strand, within CA265_RS18840 at 4687.730 kb on - strand, within CA265_RS18840 at 4687.793 kb on + strand, within CA265_RS18840 at 4687.793 kb on + strand, within CA265_RS18840 at 4687.796 kb on - strand, within CA265_RS18840 at 4687.873 kb on + strand at 4687.873 kb on + strand at 4687.874 kb on - strand at 4688.001 kb on + strand, within CA265_RS18845 at 4688.049 kb on + strand, within CA265_RS18845 at 4688.093 kb on - strand, within CA265_RS18845 at 4688.153 kb on + strand, within CA265_RS18845 at 4688.163 kb on - strand, within CA265_RS18845 at 4688.163 kb on - strand, within CA265_RS18845 at 4688.210 kb on - strand, within CA265_RS18845 at 4688.227 kb on + strand, within CA265_RS18845 at 4688.316 kb on + strand, within CA265_RS18845 at 4688.316 kb on + strand, within CA265_RS18845 at 4688.316 kb on + strand, within CA265_RS18845 at 4688.317 kb on - strand, within CA265_RS18845 at 4688.319 kb on + strand, within CA265_RS18845 at 4688.325 kb on - strand, within CA265_RS18845 at 4688.396 kb on - strand, within CA265_RS18845 at 4688.421 kb on + strand at 4688.422 kb on - strand at 4688.432 kb on - strand at 4688.432 kb on - strand at 4688.438 kb on + strand at 4688.439 kb on - strand at 4688.466 kb on - strand at 4688.541 kb on - strand, within CA265_RS18850 at 4688.557 kb on - strand, within CA265_RS18850 at 4688.679 kb on - strand at 4688.686 kb on - strand at 4688.738 kb on - strand, within CA265_RS18855 at 4688.753 kb on + strand, within CA265_RS18855 at 4688.756 kb on - strand, within CA265_RS18855 at 4688.761 kb on - strand, within CA265_RS18855 at 4688.813 kb on - strand, within CA265_RS18855 at 4688.857 kb on - strand, within CA265_RS18855 at 4688.962 kb on - strand, within CA265_RS18855 at 4689.078 kb on + strand, within CA265_RS18855 at 4689.115 kb on + strand at 4689.133 kb on - strand at 4689.145 kb on - strand at 4689.186 kb on - strand at 4689.202 kb on - strand at 4689.230 kb on + strand at 4689.231 kb on - strand at 4689.259 kb on + strand at 4689.321 kb on + strand at 4689.391 kb on + strand at 4689.391 kb on + strand at 4689.392 kb on - strand at 4689.414 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Spectinomycin 0.025 mg/ml remove 4,687,515 - CA265_RS18840 0.13 -1.0 4,687,674 + CA265_RS18840 0.49 -1.4 4,687,675 - CA265_RS18840 0.49 -0.1 4,687,708 + CA265_RS18840 0.57 +0.0 4,687,723 - CA265_RS18840 0.60 -1.3 4,687,730 - CA265_RS18840 0.62 -0.1 4,687,793 + CA265_RS18840 0.76 +0.1 4,687,793 + CA265_RS18840 0.76 -0.8 4,687,796 - CA265_RS18840 0.77 -0.4 4,687,873 + -0.8 4,687,873 + +0.2 4,687,874 - +0.8 4,688,001 + CA265_RS18845 0.18 -0.1 4,688,049 + CA265_RS18845 0.27 +0.0 4,688,093 - CA265_RS18845 0.35 +0.2 4,688,153 + CA265_RS18845 0.45 +0.6 4,688,163 - CA265_RS18845 0.47 -0.1 4,688,163 - CA265_RS18845 0.47 -1.4 4,688,210 - CA265_RS18845 0.56 +0.6 4,688,227 + CA265_RS18845 0.59 +0.9 4,688,316 + CA265_RS18845 0.75 +0.5 4,688,316 + CA265_RS18845 0.75 -0.4 4,688,316 + CA265_RS18845 0.75 +3.1 4,688,317 - CA265_RS18845 0.75 +0.5 4,688,319 + CA265_RS18845 0.75 +2.6 4,688,325 - CA265_RS18845 0.76 +0.9 4,688,396 - CA265_RS18845 0.89 +0.2 4,688,421 + +0.6 4,688,422 - +0.5 4,688,432 - -0.3 4,688,432 - +1.4 4,688,438 + -0.5 4,688,439 - +0.5 4,688,466 - +0.8 4,688,541 - CA265_RS18850 0.27 -0.5 4,688,557 - CA265_RS18850 0.35 -0.6 4,688,679 - -1.4 4,688,686 - +0.2 4,688,738 - CA265_RS18855 0.11 +0.2 4,688,753 + CA265_RS18855 0.15 +0.8 4,688,756 - CA265_RS18855 0.15 +0.4 4,688,761 - CA265_RS18855 0.16 +0.1 4,688,813 - CA265_RS18855 0.28 +1.2 4,688,857 - CA265_RS18855 0.38 -0.3 4,688,962 - CA265_RS18855 0.61 +0.0 4,689,078 + CA265_RS18855 0.87 +0.9 4,689,115 + -1.2 4,689,133 - -1.3 4,689,145 - -0.2 4,689,186 - +0.3 4,689,202 - +0.2 4,689,230 + -0.6 4,689,231 - -0.1 4,689,259 + +0.3 4,689,321 + -0.4 4,689,391 + -0.6 4,689,391 + +0.9 4,689,392 - -0.6 4,689,414 + +0.1
Or see this region's nucleotide sequence