Experiment: R2A with Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS14945 and CA265_RS14950 are separated by 49 nucleotides CA265_RS14950 and CA265_RS14955 are separated by 237 nucleotides
CA265_RS14945: CA265_RS14945 - helicase, at 3,768,843 to 3,770,147
_RS14945
CA265_RS14950: CA265_RS14950 - hypothetical protein, at 3,770,197 to 3,770,415
_RS14950
CA265_RS14955: CA265_RS14955 - hypothetical protein, at 3,770,653 to 3,771,126
_RS14955
Position (kb)
3770
3771 Strain fitness (log2 ratio)
-2
-1
0
1 at 3769.220 kb on + strand, within CA265_RS14945 at 3769.222 kb on + strand, within CA265_RS14945 at 3769.222 kb on + strand, within CA265_RS14945 at 3769.254 kb on + strand, within CA265_RS14945 at 3769.254 kb on + strand, within CA265_RS14945 at 3769.254 kb on + strand, within CA265_RS14945 at 3769.255 kb on - strand, within CA265_RS14945 at 3769.255 kb on - strand, within CA265_RS14945 at 3769.260 kb on - strand, within CA265_RS14945 at 3769.345 kb on - strand, within CA265_RS14945 at 3769.417 kb on - strand, within CA265_RS14945 at 3769.509 kb on + strand, within CA265_RS14945 at 3769.510 kb on - strand, within CA265_RS14945 at 3769.515 kb on - strand at 3769.609 kb on - strand, within CA265_RS14945 at 3769.647 kb on - strand, within CA265_RS14945 at 3769.650 kb on + strand, within CA265_RS14945 at 3769.688 kb on - strand, within CA265_RS14945 at 3769.688 kb on - strand, within CA265_RS14945 at 3769.688 kb on - strand, within CA265_RS14945 at 3769.688 kb on - strand, within CA265_RS14945 at 3769.725 kb on - strand, within CA265_RS14945 at 3769.783 kb on - strand, within CA265_RS14945 at 3769.857 kb on + strand, within CA265_RS14945 at 3769.858 kb on - strand, within CA265_RS14945 at 3769.873 kb on + strand, within CA265_RS14945 at 3769.989 kb on + strand, within CA265_RS14945 at 3770.011 kb on - strand, within CA265_RS14945 at 3770.053 kb on - strand at 3770.072 kb on + strand at 3770.106 kb on - strand at 3770.106 kb on - strand at 3770.130 kb on + strand at 3770.186 kb on + strand at 3770.186 kb on + strand at 3770.267 kb on - strand, within CA265_RS14950 at 3770.296 kb on + strand, within CA265_RS14950 at 3770.462 kb on + strand at 3770.479 kb on + strand at 3770.484 kb on - strand at 3770.561 kb on + strand at 3770.645 kb on + strand at 3770.652 kb on + strand at 3770.652 kb on + strand at 3770.810 kb on + strand, within CA265_RS14955 at 3770.810 kb on + strand, within CA265_RS14955 at 3770.810 kb on + strand, within CA265_RS14955 at 3770.810 kb on + strand, within CA265_RS14955 at 3770.811 kb on - strand, within CA265_RS14955 at 3770.880 kb on - strand, within CA265_RS14955 at 3770.949 kb on - strand, within CA265_RS14955 at 3770.979 kb on - strand, within CA265_RS14955 at 3771.046 kb on - strand, within CA265_RS14955 at 3771.054 kb on + strand, within CA265_RS14955 at 3771.069 kb on + strand, within CA265_RS14955 at 3771.100 kb on - strand at 3771.125 kb on - strand at 3771.128 kb on + strand at 3771.278 kb on - strand at 3771.327 kb on - strand at 3771.338 kb on - strand at 3771.395 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Spectinomycin 0.025 mg/ml remove 3,769,220 + CA265_RS14945 0.29 +0.6 3,769,222 + CA265_RS14945 0.29 +1.0 3,769,222 + CA265_RS14945 0.29 +0.3 3,769,254 + CA265_RS14945 0.31 +0.8 3,769,254 + CA265_RS14945 0.31 -1.5 3,769,254 + CA265_RS14945 0.31 -0.1 3,769,255 - CA265_RS14945 0.32 -0.1 3,769,255 - CA265_RS14945 0.32 -0.3 3,769,260 - CA265_RS14945 0.32 +0.6 3,769,345 - CA265_RS14945 0.38 +0.0 3,769,417 - CA265_RS14945 0.44 -0.2 3,769,509 + CA265_RS14945 0.51 -1.1 3,769,510 - CA265_RS14945 0.51 -0.2 3,769,515 - -0.6 3,769,609 - CA265_RS14945 0.59 -0.0 3,769,647 - CA265_RS14945 0.62 -0.3 3,769,650 + CA265_RS14945 0.62 -0.8 3,769,688 - CA265_RS14945 0.65 -2.1 3,769,688 - CA265_RS14945 0.65 -0.2 3,769,688 - CA265_RS14945 0.65 +0.4 3,769,688 - CA265_RS14945 0.65 +0.2 3,769,725 - CA265_RS14945 0.68 -0.5 3,769,783 - CA265_RS14945 0.72 +0.8 3,769,857 + CA265_RS14945 0.78 -0.2 3,769,858 - CA265_RS14945 0.78 -0.6 3,769,873 + CA265_RS14945 0.79 +0.3 3,769,989 + CA265_RS14945 0.88 +1.1 3,770,011 - CA265_RS14945 0.90 +0.1 3,770,053 - +0.2 3,770,072 + -0.7 3,770,106 - -1.5 3,770,106 - -0.3 3,770,130 + +0.2 3,770,186 + +0.2 3,770,186 + -0.7 3,770,267 - CA265_RS14950 0.32 -1.0 3,770,296 + CA265_RS14950 0.45 -0.3 3,770,462 + +0.4 3,770,479 + +0.1 3,770,484 - -0.2 3,770,561 + +0.2 3,770,645 + -0.3 3,770,652 + +0.2 3,770,652 + +0.2 3,770,810 + CA265_RS14955 0.33 -1.0 3,770,810 + CA265_RS14955 0.33 +0.3 3,770,810 + CA265_RS14955 0.33 +1.0 3,770,810 + CA265_RS14955 0.33 +0.2 3,770,811 - CA265_RS14955 0.33 +0.1 3,770,880 - CA265_RS14955 0.48 -0.2 3,770,949 - CA265_RS14955 0.62 -0.0 3,770,979 - CA265_RS14955 0.69 +0.5 3,771,046 - CA265_RS14955 0.83 +0.4 3,771,054 + CA265_RS14955 0.85 +0.1 3,771,069 + CA265_RS14955 0.88 +0.6 3,771,100 - +0.3 3,771,125 - -0.1 3,771,128 + +0.2 3,771,278 - +0.9 3,771,327 - -0.1 3,771,338 - -0.1 3,771,395 + -0.9
Or see this region's nucleotide sequence