Experiment: R2A with Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS04805 and CA265_RS04810 are separated by 7 nucleotides CA265_RS04810 and CA265_RS04815 are separated by 318 nucleotides CA265_RS04815 and CA265_RS04820 are separated by 402 nucleotides
CA265_RS04805: CA265_RS04805 - XynC protein, at 1,144,731 to 1,145,549
_RS04805
CA265_RS04810: CA265_RS04810 - response regulator, at 1,145,557 to 1,145,919
_RS04810
CA265_RS04815: CA265_RS04815 - hypothetical protein, at 1,146,238 to 1,146,492
_RS04815
CA265_RS04820: CA265_RS04820 - GntR family transcriptional regulator, at 1,146,895 to 1,147,599
_RS04820
Position (kb)
1146
1147 Strain fitness (log2 ratio)
-2
-1
0
1 at 1145.261 kb on + strand, within CA265_RS04805 at 1145.334 kb on + strand, within CA265_RS04805 at 1145.342 kb on + strand, within CA265_RS04805 at 1145.438 kb on + strand, within CA265_RS04805 at 1145.484 kb on + strand at 1145.530 kb on - strand at 1145.552 kb on - strand at 1145.579 kb on - strand at 1145.640 kb on + strand, within CA265_RS04810 at 1145.698 kb on + strand, within CA265_RS04810 at 1145.698 kb on + strand, within CA265_RS04810 at 1145.698 kb on + strand, within CA265_RS04810 at 1145.698 kb on + strand, within CA265_RS04810 at 1145.728 kb on - strand, within CA265_RS04810 at 1145.756 kb on - strand, within CA265_RS04810 at 1145.756 kb on - strand, within CA265_RS04810 at 1145.756 kb on - strand, within CA265_RS04810 at 1145.776 kb on + strand, within CA265_RS04810 at 1145.869 kb on + strand, within CA265_RS04810 at 1146.003 kb on - strand at 1146.062 kb on + strand at 1146.087 kb on - strand at 1146.120 kb on - strand at 1146.149 kb on - strand at 1146.157 kb on + strand at 1146.158 kb on - strand at 1146.177 kb on + strand at 1146.177 kb on + strand at 1146.248 kb on + strand at 1146.408 kb on + strand, within CA265_RS04815 at 1146.409 kb on - strand, within CA265_RS04815 at 1146.428 kb on + strand, within CA265_RS04815 at 1146.461 kb on + strand, within CA265_RS04815 at 1146.462 kb on - strand, within CA265_RS04815 at 1146.522 kb on + strand at 1146.523 kb on - strand at 1146.566 kb on + strand at 1146.666 kb on + strand at 1146.667 kb on - strand at 1146.671 kb on - strand at 1146.671 kb on - strand at 1146.754 kb on - strand at 1146.754 kb on - strand at 1146.757 kb on + strand at 1146.758 kb on - strand at 1146.789 kb on - strand at 1146.794 kb on + strand at 1146.797 kb on + strand at 1146.798 kb on - strand at 1146.829 kb on - strand at 1146.843 kb on + strand at 1146.844 kb on - strand at 1146.859 kb on - strand at 1146.882 kb on - strand at 1147.000 kb on - strand, within CA265_RS04820 at 1147.073 kb on - strand, within CA265_RS04820 at 1147.075 kb on + strand, within CA265_RS04820 at 1147.117 kb on - strand, within CA265_RS04820 at 1147.117 kb on - strand, within CA265_RS04820 at 1147.317 kb on + strand, within CA265_RS04820 at 1147.400 kb on - strand, within CA265_RS04820 at 1147.438 kb on + strand, within CA265_RS04820 at 1147.438 kb on + strand, within CA265_RS04820 at 1147.439 kb on - strand, within CA265_RS04820 at 1147.450 kb on + strand, within CA265_RS04820 at 1147.491 kb on + strand, within CA265_RS04820
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Spectinomycin 0.025 mg/ml remove 1,145,261 + CA265_RS04805 0.65 -0.7 1,145,334 + CA265_RS04805 0.74 +0.5 1,145,342 + CA265_RS04805 0.75 -0.3 1,145,438 + CA265_RS04805 0.86 +0.1 1,145,484 + -0.8 1,145,530 - +0.7 1,145,552 - -0.1 1,145,579 - +0.1 1,145,640 + CA265_RS04810 0.23 -1.6 1,145,698 + CA265_RS04810 0.39 -1.3 1,145,698 + CA265_RS04810 0.39 +0.1 1,145,698 + CA265_RS04810 0.39 +0.5 1,145,698 + CA265_RS04810 0.39 +0.7 1,145,728 - CA265_RS04810 0.47 -0.3 1,145,756 - CA265_RS04810 0.55 +0.5 1,145,756 - CA265_RS04810 0.55 +0.4 1,145,756 - CA265_RS04810 0.55 -0.1 1,145,776 + CA265_RS04810 0.60 +0.5 1,145,869 + CA265_RS04810 0.86 -0.8 1,146,003 - +0.4 1,146,062 + -0.8 1,146,087 - -2.4 1,146,120 - +0.1 1,146,149 - -0.1 1,146,157 + -0.3 1,146,158 - -0.3 1,146,177 + -0.5 1,146,177 + -0.4 1,146,248 + +0.4 1,146,408 + CA265_RS04815 0.67 -0.6 1,146,409 - CA265_RS04815 0.67 +0.3 1,146,428 + CA265_RS04815 0.75 -0.5 1,146,461 + CA265_RS04815 0.87 -1.9 1,146,462 - CA265_RS04815 0.88 -0.8 1,146,522 + -0.1 1,146,523 - +0.0 1,146,566 + -0.8 1,146,666 + -0.1 1,146,667 - -0.6 1,146,671 - -0.7 1,146,671 - -0.2 1,146,754 - -1.0 1,146,754 - -1.4 1,146,757 + +0.2 1,146,758 - +0.0 1,146,789 - -1.4 1,146,794 + -0.6 1,146,797 + -0.1 1,146,798 - -0.2 1,146,829 - -1.1 1,146,843 + +0.3 1,146,844 - -0.0 1,146,859 - +0.5 1,146,882 - -0.5 1,147,000 - CA265_RS04820 0.15 -0.8 1,147,073 - CA265_RS04820 0.25 -0.1 1,147,075 + CA265_RS04820 0.26 -0.5 1,147,117 - CA265_RS04820 0.31 -1.5 1,147,117 - CA265_RS04820 0.31 -0.7 1,147,317 + CA265_RS04820 0.60 -1.0 1,147,400 - CA265_RS04820 0.72 +1.3 1,147,438 + CA265_RS04820 0.77 -0.5 1,147,438 + CA265_RS04820 0.77 -0.8 1,147,439 - CA265_RS04820 0.77 +0.5 1,147,450 + CA265_RS04820 0.79 +1.1 1,147,491 + CA265_RS04820 0.85 -0.3
Or see this region's nucleotide sequence