Experiment: R2A with Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS02105 and CA265_RS02110 overlap by 4 nucleotides CA265_RS02110 and CA265_RS02115 are separated by 20 nucleotides CA265_RS02115 and CA265_RS02120 are separated by 3 nucleotides CA265_RS02120 and CA265_RS02125 are separated by 4 nucleotides CA265_RS02125 and CA265_RS02130 are separated by 6 nucleotides
CA265_RS02105: CA265_RS02105 - hypothetical protein, at 530,312 to 530,746
_RS02105
CA265_RS02110: CA265_RS02110 - hypothetical protein, at 530,743 to 531,192
_RS02110
CA265_RS02115: CA265_RS02115 - Fur family transcriptional regulator, at 531,213 to 531,650
_RS02115
CA265_RS02120: CA265_RS02120 - acyl transferase, at 531,654 to 532,652
_RS02120
CA265_RS02125: CA265_RS02125 - hypothetical protein, at 532,657 to 533,016
_RS02125
CA265_RS02130: CA265_RS02130 - penicillin acylase family protein, at 533,023 to 535,452
_RS02130
Position (kb) 531
532
533 Strain fitness (log2 ratio) -2
-1
0
1
2 at 530.754 kb on - strand at 530.756 kb on - strand at 530.756 kb on - strand at 530.784 kb on + strand at 530.892 kb on + strand, within CA265_RS02110 at 530.892 kb on + strand, within CA265_RS02110 at 531.026 kb on - strand, within CA265_RS02110 at 531.089 kb on - strand, within CA265_RS02110 at 531.091 kb on - strand, within CA265_RS02110 at 531.191 kb on - strand at 531.198 kb on + strand at 531.199 kb on - strand at 531.207 kb on - strand at 531.224 kb on + strand at 531.334 kb on - strand, within CA265_RS02115 at 531.379 kb on - strand, within CA265_RS02115 at 531.379 kb on - strand, within CA265_RS02115 at 531.428 kb on - strand, within CA265_RS02115 at 531.450 kb on + strand, within CA265_RS02115 at 531.450 kb on + strand, within CA265_RS02115 at 531.451 kb on - strand, within CA265_RS02115 at 531.502 kb on + strand, within CA265_RS02115 at 531.502 kb on + strand, within CA265_RS02115 at 531.503 kb on - strand, within CA265_RS02115 at 531.510 kb on - strand, within CA265_RS02115 at 531.585 kb on - strand, within CA265_RS02115 at 531.643 kb on + strand at 531.643 kb on + strand at 531.643 kb on + strand at 531.655 kb on + strand at 531.671 kb on + strand at 531.733 kb on - strand at 531.798 kb on - strand, within CA265_RS02120 at 531.904 kb on + strand, within CA265_RS02120 at 531.919 kb on - strand, within CA265_RS02120 at 531.934 kb on + strand, within CA265_RS02120 at 531.942 kb on - strand, within CA265_RS02120 at 532.076 kb on + strand, within CA265_RS02120 at 532.076 kb on + strand, within CA265_RS02120 at 532.094 kb on - strand, within CA265_RS02120 at 532.169 kb on + strand, within CA265_RS02120 at 532.174 kb on - strand, within CA265_RS02120 at 532.227 kb on - strand, within CA265_RS02120 at 532.228 kb on + strand, within CA265_RS02120 at 532.229 kb on - strand, within CA265_RS02120 at 532.327 kb on + strand, within CA265_RS02120 at 532.327 kb on + strand, within CA265_RS02120 at 532.357 kb on - strand, within CA265_RS02120 at 532.357 kb on - strand, within CA265_RS02120 at 532.385 kb on + strand, within CA265_RS02120 at 532.424 kb on + strand, within CA265_RS02120 at 532.513 kb on - strand, within CA265_RS02120 at 532.526 kb on - strand, within CA265_RS02120 at 532.558 kb on - strand at 532.615 kb on - strand at 532.622 kb on + strand at 532.622 kb on + strand at 532.623 kb on - strand at 532.623 kb on - strand at 532.649 kb on - strand at 532.649 kb on - strand at 532.658 kb on + strand at 532.718 kb on + strand, within CA265_RS02125 at 532.819 kb on + strand, within CA265_RS02125 at 532.831 kb on - strand, within CA265_RS02125 at 532.846 kb on + strand, within CA265_RS02125 at 532.847 kb on - strand, within CA265_RS02125 at 532.994 kb on - strand at 532.996 kb on + strand at 533.001 kb on + strand at 533.019 kb on + strand at 533.050 kb on - strand at 533.104 kb on - strand at 533.128 kb on - strand at 533.131 kb on - strand at 533.136 kb on - strand at 533.246 kb on - strand at 533.253 kb on + strand at 533.253 kb on + strand at 533.254 kb on - strand at 533.254 kb on - strand at 533.256 kb on + strand at 533.257 kb on - strand at 533.324 kb on - strand, within CA265_RS02130 at 533.387 kb on - strand, within CA265_RS02130 at 533.392 kb on - strand, within CA265_RS02130 at 533.431 kb on + strand, within CA265_RS02130 at 533.431 kb on + strand, within CA265_RS02130 at 533.569 kb on - strand, within CA265_RS02130 at 533.651 kb on + strand, within CA265_RS02130 at 533.652 kb on - strand, within CA265_RS02130
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Spectinomycin 0.025 mg/ml remove 530,754 - +0.1 530,756 - -1.1 530,756 - +0.1 530,784 + +0.0 530,892 + CA265_RS02110 0.33 -0.7 530,892 + CA265_RS02110 0.33 +0.1 531,026 - CA265_RS02110 0.63 -0.6 531,089 - CA265_RS02110 0.77 -0.1 531,091 - CA265_RS02110 0.77 +0.6 531,191 - +0.6 531,198 + -0.2 531,199 - -0.9 531,207 - -0.7 531,224 + -0.3 531,334 - CA265_RS02115 0.28 +0.1 531,379 - CA265_RS02115 0.38 -0.7 531,379 - CA265_RS02115 0.38 +0.5 531,428 - CA265_RS02115 0.49 -0.9 531,450 + CA265_RS02115 0.54 +0.1 531,450 + CA265_RS02115 0.54 -0.6 531,451 - CA265_RS02115 0.54 -0.0 531,502 + CA265_RS02115 0.66 -0.8 531,502 + CA265_RS02115 0.66 +0.2 531,503 - CA265_RS02115 0.66 -1.4 531,510 - CA265_RS02115 0.68 -1.7 531,585 - CA265_RS02115 0.85 -0.9 531,643 + -0.3 531,643 + +1.5 531,643 + +0.8 531,655 + -0.4 531,671 + +0.4 531,733 - -0.8 531,798 - CA265_RS02120 0.14 -1.3 531,904 + CA265_RS02120 0.25 -0.7 531,919 - CA265_RS02120 0.27 -1.5 531,934 + CA265_RS02120 0.28 -1.0 531,942 - CA265_RS02120 0.29 -0.6 532,076 + CA265_RS02120 0.42 -0.7 532,076 + CA265_RS02120 0.42 -2.4 532,094 - CA265_RS02120 0.44 +1.7 532,169 + CA265_RS02120 0.52 -0.2 532,174 - CA265_RS02120 0.52 -0.3 532,227 - CA265_RS02120 0.57 -0.7 532,228 + CA265_RS02120 0.57 -1.1 532,229 - CA265_RS02120 0.58 -0.4 532,327 + CA265_RS02120 0.67 +2.5 532,327 + CA265_RS02120 0.67 -1.9 532,357 - CA265_RS02120 0.70 -1.4 532,357 - CA265_RS02120 0.70 -1.1 532,385 + CA265_RS02120 0.73 -0.9 532,424 + CA265_RS02120 0.77 -1.1 532,513 - CA265_RS02120 0.86 -1.9 532,526 - CA265_RS02120 0.87 -0.3 532,558 - -0.6 532,615 - -1.2 532,622 + -0.6 532,622 + -0.8 532,623 - -0.7 532,623 - -0.7 532,649 - +0.3 532,649 - +0.1 532,658 + -0.4 532,718 + CA265_RS02125 0.17 -0.2 532,819 + CA265_RS02125 0.45 +0.2 532,831 - CA265_RS02125 0.48 -1.0 532,846 + CA265_RS02125 0.53 -0.2 532,847 - CA265_RS02125 0.53 -0.6 532,994 - +1.1 532,996 + -0.3 533,001 + +0.1 533,019 + -0.2 533,050 - +0.1 533,104 - +0.5 533,128 - -0.1 533,131 - -0.5 533,136 - -1.1 533,246 - +0.4 533,253 + -0.3 533,253 + -0.3 533,254 - -0.3 533,254 - -0.5 533,256 + +0.2 533,257 - -0.8 533,324 - CA265_RS02130 0.12 +0.0 533,387 - CA265_RS02130 0.15 +0.2 533,392 - CA265_RS02130 0.15 -0.2 533,431 + CA265_RS02130 0.17 -1.3 533,431 + CA265_RS02130 0.17 +0.3 533,569 - CA265_RS02130 0.22 -1.2 533,651 + CA265_RS02130 0.26 +0.0 533,652 - CA265_RS02130 0.26 +1.3
Or see this region's nucleotide sequence