Experiment: R2A with Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS01950 and CA265_RS01955 overlap by 14 nucleotides CA265_RS01955 and CA265_RS01960 are separated by 459 nucleotides CA265_RS01960 and CA265_RS01965 overlap by 11 nucleotides CA265_RS01965 and CA265_RS01970 overlap by 4 nucleotides
CA265_RS01950: CA265_RS01950 - hypothetical protein, at 496,322 to 496,552
_RS01950
CA265_RS01955: CA265_RS01955 - VapC toxin family PIN domain ribonuclease, at 496,539 to 496,930
_RS01955
CA265_RS01960: CA265_RS01960 - division/cell wall cluster transcriptional repressor MraZ, at 497,390 to 497,857
_RS01960
CA265_RS01965: CA265_RS01965 - 16S rRNA (cytosine(1402)-N(4))-methyltransferase, at 497,847 to 498,752
_RS01965
CA265_RS01970: CA265_RS01970 - hypothetical protein, at 498,749 to 499,171
_RS01970
Position (kb) 497
498 Strain fitness (log2 ratio) -2
-1
0
1 at 496.408 kb on - strand, within CA265_RS01950 at 496.477 kb on + strand, within CA265_RS01950 at 496.504 kb on + strand, within CA265_RS01950 at 496.504 kb on + strand, within CA265_RS01950 at 496.504 kb on + strand, within CA265_RS01950 at 496.550 kb on + strand at 496.558 kb on + strand at 496.558 kb on + strand at 496.559 kb on - strand at 496.565 kb on + strand at 496.565 kb on + strand at 496.568 kb on + strand at 496.568 kb on + strand at 496.569 kb on - strand at 496.569 kb on - strand at 496.569 kb on - strand at 496.569 kb on - strand at 496.583 kb on - strand, within CA265_RS01955 at 496.668 kb on - strand, within CA265_RS01955 at 496.668 kb on - strand, within CA265_RS01955 at 496.738 kb on + strand, within CA265_RS01955 at 496.831 kb on + strand, within CA265_RS01955 at 496.848 kb on + strand, within CA265_RS01955 at 496.849 kb on - strand, within CA265_RS01955 at 496.913 kb on - strand at 497.138 kb on - strand at 497.156 kb on - strand at 497.167 kb on - strand at 497.220 kb on + strand at 497.221 kb on - strand at 497.263 kb on + strand at 497.263 kb on + strand at 497.274 kb on + strand at 497.289 kb on - strand at 497.500 kb on + strand, within CA265_RS01960
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Spectinomycin 0.025 mg/ml remove 496,408 - CA265_RS01950 0.37 -0.4 496,477 + CA265_RS01950 0.67 +0.2 496,504 + CA265_RS01950 0.79 +0.0 496,504 + CA265_RS01950 0.79 -0.1 496,504 + CA265_RS01950 0.79 +1.1 496,550 + +0.3 496,558 + -0.5 496,558 + +0.1 496,559 - -0.2 496,565 + +0.3 496,565 + +0.5 496,568 + -2.0 496,568 + -0.2 496,569 - -0.3 496,569 - -0.1 496,569 - +0.5 496,569 - +0.5 496,583 - CA265_RS01955 0.11 -0.3 496,668 - CA265_RS01955 0.33 +0.6 496,668 - CA265_RS01955 0.33 -0.2 496,738 + CA265_RS01955 0.51 -0.6 496,831 + CA265_RS01955 0.74 +0.4 496,848 + CA265_RS01955 0.79 -1.2 496,849 - CA265_RS01955 0.79 -0.9 496,913 - -0.1 497,138 - -0.9 497,156 - +0.2 497,167 - -1.0 497,220 + -1.0 497,221 - -0.2 497,263 + -0.3 497,263 + -0.1 497,274 + -0.4 497,289 - -0.2 497,500 + CA265_RS01960 0.24 -0.7
Or see this region's nucleotide sequence