Experiment: R2A with Spectinomycin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS00660 and CA265_RS00665 are separated by 8 nucleotides CA265_RS00665 and CA265_RS00670 are separated by 494 nucleotides
CA265_RS00660: CA265_RS00660 - uvrA paralog in Bacteroidetes (from data) , at 148,916 to 151,729
_RS00660
CA265_RS00665: CA265_RS00665 - lysine transporter LysE, at 151,738 to 152,391
_RS00665
CA265_RS00670: CA265_RS00670 - metallophosphoesterase, at 152,886 to 153,728
_RS00670
Position (kb)
151
152
153 Strain fitness (log2 ratio)
-2
-1
0
1 at 150.763 kb on + strand, within CA265_RS00660 at 150.801 kb on + strand, within CA265_RS00660 at 150.802 kb on - strand, within CA265_RS00660 at 150.905 kb on + strand, within CA265_RS00660 at 150.906 kb on - strand, within CA265_RS00660 at 150.927 kb on - strand, within CA265_RS00660 at 150.946 kb on - strand, within CA265_RS00660 at 151.001 kb on + strand, within CA265_RS00660 at 151.002 kb on - strand, within CA265_RS00660 at 151.044 kb on + strand, within CA265_RS00660 at 151.095 kb on + strand, within CA265_RS00660 at 151.095 kb on + strand, within CA265_RS00660 at 151.096 kb on - strand, within CA265_RS00660 at 151.229 kb on + strand, within CA265_RS00660 at 151.246 kb on - strand, within CA265_RS00660 at 151.321 kb on - strand, within CA265_RS00660 at 151.338 kb on + strand, within CA265_RS00660 at 151.339 kb on - strand, within CA265_RS00660 at 151.372 kb on + strand, within CA265_RS00660 at 151.376 kb on - strand, within CA265_RS00660 at 151.376 kb on - strand, within CA265_RS00660 at 151.376 kb on - strand, within CA265_RS00660 at 151.435 kb on + strand, within CA265_RS00660 at 151.457 kb on - strand at 151.523 kb on - strand at 151.523 kb on - strand at 151.523 kb on - strand at 151.523 kb on - strand at 151.567 kb on + strand at 151.568 kb on - strand at 151.568 kb on - strand at 151.666 kb on - strand at 151.787 kb on - strand at 151.804 kb on - strand, within CA265_RS00665 at 151.834 kb on - strand, within CA265_RS00665 at 151.859 kb on - strand, within CA265_RS00665 at 151.859 kb on - strand, within CA265_RS00665 at 152.080 kb on - strand, within CA265_RS00665 at 152.164 kb on + strand, within CA265_RS00665 at 152.279 kb on - strand, within CA265_RS00665 at 152.388 kb on - strand at 152.390 kb on - strand at 152.390 kb on - strand at 152.394 kb on + strand at 152.460 kb on + strand at 152.460 kb on + strand at 152.522 kb on + strand at 152.578 kb on + strand at 152.578 kb on + strand at 152.690 kb on - strand at 152.744 kb on - strand at 152.788 kb on - strand at 152.788 kb on - strand at 152.791 kb on + strand at 152.813 kb on + strand at 152.813 kb on + strand at 152.814 kb on - strand at 152.831 kb on + strand at 152.852 kb on + strand at 152.865 kb on + strand at 152.875 kb on - strand at 152.885 kb on + strand at 152.885 kb on + strand at 152.932 kb on + strand at 152.932 kb on + strand at 152.933 kb on - strand at 152.942 kb on + strand at 153.008 kb on + strand, within CA265_RS00670 at 153.063 kb on + strand, within CA265_RS00670 at 153.066 kb on - strand, within CA265_RS00670 at 153.084 kb on - strand, within CA265_RS00670 at 153.140 kb on + strand, within CA265_RS00670 at 153.172 kb on + strand, within CA265_RS00670 at 153.173 kb on - strand, within CA265_RS00670 at 153.173 kb on - strand, within CA265_RS00670 at 153.198 kb on + strand, within CA265_RS00670 at 153.209 kb on + strand, within CA265_RS00670 at 153.316 kb on + strand, within CA265_RS00670
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Spectinomycin 0.025 mg/ml remove 150,763 + CA265_RS00660 0.66 -0.8 150,801 + CA265_RS00660 0.67 +0.8 150,802 - CA265_RS00660 0.67 -0.1 150,905 + CA265_RS00660 0.71 -0.8 150,906 - CA265_RS00660 0.71 -0.4 150,927 - CA265_RS00660 0.71 -0.4 150,946 - CA265_RS00660 0.72 -0.6 151,001 + CA265_RS00660 0.74 -0.5 151,002 - CA265_RS00660 0.74 -1.0 151,044 + CA265_RS00660 0.76 -0.6 151,095 + CA265_RS00660 0.77 -0.8 151,095 + CA265_RS00660 0.77 +0.8 151,096 - CA265_RS00660 0.77 -1.3 151,229 + CA265_RS00660 0.82 -0.5 151,246 - CA265_RS00660 0.83 -0.2 151,321 - CA265_RS00660 0.85 +0.1 151,338 + CA265_RS00660 0.86 +0.3 151,339 - CA265_RS00660 0.86 +0.1 151,372 + CA265_RS00660 0.87 -0.0 151,376 - CA265_RS00660 0.87 -0.5 151,376 - CA265_RS00660 0.87 -0.3 151,376 - CA265_RS00660 0.87 -0.1 151,435 + CA265_RS00660 0.90 -0.8 151,457 - -0.4 151,523 - +0.2 151,523 - +0.3 151,523 - +0.3 151,523 - -0.5 151,567 + -0.8 151,568 - -0.6 151,568 - +0.1 151,666 - +0.2 151,787 - -0.9 151,804 - CA265_RS00665 0.10 -1.8 151,834 - CA265_RS00665 0.15 -0.0 151,859 - CA265_RS00665 0.19 +0.3 151,859 - CA265_RS00665 0.19 -1.9 152,080 - CA265_RS00665 0.52 -0.5 152,164 + CA265_RS00665 0.65 -1.5 152,279 - CA265_RS00665 0.83 -0.2 152,388 - -0.3 152,390 - -0.1 152,390 - +1.4 152,394 + -0.0 152,460 + -1.2 152,460 + -0.5 152,522 + +0.6 152,578 + -0.2 152,578 + -0.4 152,690 - -0.3 152,744 - +1.7 152,788 - -0.2 152,788 - +0.7 152,791 + +0.3 152,813 + -0.5 152,813 + +0.6 152,814 - +0.2 152,831 + +0.3 152,852 + +0.3 152,865 + +0.0 152,875 - -1.4 152,885 + -0.1 152,885 + -0.2 152,932 + +0.2 152,932 + -0.6 152,933 - -2.2 152,942 + -0.5 153,008 + CA265_RS00670 0.14 +0.3 153,063 + CA265_RS00670 0.21 -0.2 153,066 - CA265_RS00670 0.21 -1.7 153,084 - CA265_RS00670 0.23 -0.2 153,140 + CA265_RS00670 0.30 +0.1 153,172 + CA265_RS00670 0.34 +0.2 153,173 - CA265_RS00670 0.34 -0.2 153,173 - CA265_RS00670 0.34 -0.3 153,198 + CA265_RS00670 0.37 -0.4 153,209 + CA265_RS00670 0.38 +0.4 153,316 + CA265_RS00670 0.51 +0.0
Or see this region's nucleotide sequence