Experiment: D-Raffinose (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2074 and BT2075 are separated by 28 nucleotides BT2075 and BT2076 overlap by 1 nucleotides BT2076 and BT2077 are separated by 18 nucleotides
BT2074: BT2074 - ketol-acid reductoisomerase (NCBI ptt file), at 2,599,081 to 2,600,160
BT2074
BT2075: BT2075 - acyl-ACP thioesterase (NCBI ptt file), at 2,600,189 to 2,600,932
BT2075
BT2076: BT2076 - acetohydroxybutanoate synthase regulatory subunit (EC 2.2.1.6) (from data) , at 2,600,932 to 2,601,495
BT2076
BT2077: BT2077 - acetolactate synthase large subunit (NCBI ptt file), at 2,601,514 to 2,603,211
BT2077
Position (kb)
2600
2601
2602 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2599.957 kb on + strand, within BT2074 at 2599.958 kb on - strand, within BT2074 at 2599.958 kb on - strand, within BT2074 at 2600.087 kb on - strand at 2600.087 kb on - strand at 2600.165 kb on + strand at 2600.181 kb on - strand at 2600.186 kb on - strand at 2600.187 kb on + strand at 2600.188 kb on - strand at 2600.216 kb on + strand at 2600.216 kb on + strand at 2600.216 kb on + strand at 2600.220 kb on - strand at 2600.247 kb on - strand at 2600.319 kb on + strand, within BT2075 at 2600.319 kb on + strand, within BT2075 at 2600.319 kb on + strand, within BT2075 at 2600.320 kb on - strand, within BT2075 at 2600.342 kb on - strand, within BT2075 at 2600.356 kb on - strand, within BT2075 at 2600.381 kb on + strand, within BT2075 at 2600.520 kb on - strand, within BT2075 at 2600.521 kb on + strand, within BT2075 at 2600.521 kb on + strand, within BT2075 at 2600.522 kb on - strand, within BT2075 at 2600.524 kb on - strand, within BT2075 at 2600.710 kb on - strand, within BT2075 at 2600.763 kb on + strand, within BT2075 at 2600.764 kb on - strand, within BT2075 at 2600.782 kb on + strand, within BT2075 at 2600.782 kb on + strand, within BT2075 at 2600.782 kb on + strand, within BT2075 at 2600.783 kb on - strand, within BT2075 at 2600.904 kb on + strand at 2600.934 kb on - strand at 2601.015 kb on + strand, within BT2076 at 2601.015 kb on + strand, within BT2076 at 2601.015 kb on + strand, within BT2076 at 2601.087 kb on + strand, within BT2076 at 2601.143 kb on - strand, within BT2076 at 2601.156 kb on + strand, within BT2076 at 2601.158 kb on + strand, within BT2076 at 2601.159 kb on - strand, within BT2076 at 2601.208 kb on - strand, within BT2076 at 2601.216 kb on + strand, within BT2076 at 2601.217 kb on - strand, within BT2076 at 2601.218 kb on + strand, within BT2076 at 2601.218 kb on + strand, within BT2076 at 2601.218 kb on + strand, within BT2076 at 2601.218 kb on + strand, within BT2076 at 2601.219 kb on - strand, within BT2076 at 2601.220 kb on + strand, within BT2076 at 2601.220 kb on + strand, within BT2076 at 2601.261 kb on - strand, within BT2076 at 2601.345 kb on + strand, within BT2076 at 2601.351 kb on + strand, within BT2076 at 2601.355 kb on + strand, within BT2076 at 2601.400 kb on + strand, within BT2076 at 2601.466 kb on + strand at 2601.496 kb on - strand at 2601.496 kb on - strand at 2601.496 kb on - strand at 2601.681 kb on + strand at 2601.978 kb on + strand, within BT2077 at 2601.978 kb on + strand, within BT2077 at 2601.991 kb on - strand, within BT2077 at 2601.996 kb on + strand, within BT2077 at 2602.030 kb on + strand, within BT2077 at 2602.031 kb on - strand, within BT2077 at 2602.267 kb on + strand, within BT2077 at 2602.268 kb on - strand, within BT2077 at 2602.268 kb on - strand, within BT2077 at 2602.279 kb on + strand, within BT2077 at 2602.333 kb on - strand, within BT2077 at 2602.368 kb on + strand, within BT2077 at 2602.385 kb on - strand, within BT2077 at 2602.398 kb on + strand, within BT2077 at 2602.401 kb on + strand, within BT2077 at 2602.402 kb on - strand, within BT2077 at 2602.402 kb on - strand, within BT2077 at 2602.491 kb on + strand, within BT2077
Per-strain Table
Position Strand Gene LocusTag Fraction D-Raffinose (C) remove 2,599,957 + BT2074 0.81 -3.3 2,599,958 - BT2074 0.81 -1.7 2,599,958 - BT2074 0.81 -1.0 2,600,087 - -2.6 2,600,087 - -2.0 2,600,165 + -3.2 2,600,181 - +1.0 2,600,186 - +0.5 2,600,187 + -2.6 2,600,188 - -1.8 2,600,216 + -2.1 2,600,216 + -2.3 2,600,216 + -4.1 2,600,220 - +0.5 2,600,247 - +0.6 2,600,319 + BT2075 0.17 -1.3 2,600,319 + BT2075 0.17 -2.3 2,600,319 + BT2075 0.17 -3.6 2,600,320 - BT2075 0.18 +1.0 2,600,342 - BT2075 0.21 +0.5 2,600,356 - BT2075 0.22 +1.7 2,600,381 + BT2075 0.26 -2.6 2,600,520 - BT2075 0.44 -0.1 2,600,521 + BT2075 0.45 -2.8 2,600,521 + BT2075 0.45 -1.6 2,600,522 - BT2075 0.45 +0.4 2,600,524 - BT2075 0.45 +1.2 2,600,710 - BT2075 0.70 +0.4 2,600,763 + BT2075 0.77 -4.4 2,600,764 - BT2075 0.77 +0.1 2,600,782 + BT2075 0.80 -4.0 2,600,782 + BT2075 0.80 -0.4 2,600,782 + BT2075 0.80 -3.8 2,600,783 - BT2075 0.80 +0.6 2,600,904 + -2.1 2,600,934 - -2.1 2,601,015 + BT2076 0.15 -4.8 2,601,015 + BT2076 0.15 -2.7 2,601,015 + BT2076 0.15 -3.9 2,601,087 + BT2076 0.27 -3.8 2,601,143 - BT2076 0.37 -2.0 2,601,156 + BT2076 0.40 -2.4 2,601,158 + BT2076 0.40 -3.1 2,601,159 - BT2076 0.40 -4.0 2,601,208 - BT2076 0.49 -3.2 2,601,216 + BT2076 0.50 -3.6 2,601,217 - BT2076 0.51 -3.1 2,601,218 + BT2076 0.51 -3.4 2,601,218 + BT2076 0.51 -3.3 2,601,218 + BT2076 0.51 -2.8 2,601,218 + BT2076 0.51 -1.7 2,601,219 - BT2076 0.51 -2.2 2,601,220 + BT2076 0.51 -0.8 2,601,220 + BT2076 0.51 -4.0 2,601,261 - BT2076 0.58 -1.5 2,601,345 + BT2076 0.73 -3.6 2,601,351 + BT2076 0.74 -3.3 2,601,355 + BT2076 0.75 -2.9 2,601,400 + BT2076 0.83 -2.8 2,601,466 + -2.9 2,601,496 - -0.4 2,601,496 - -2.8 2,601,496 - -3.5 2,601,681 + -4.0 2,601,978 + BT2077 0.27 -1.4 2,601,978 + BT2077 0.27 -1.6 2,601,991 - BT2077 0.28 -0.7 2,601,996 + BT2077 0.28 -4.5 2,602,030 + BT2077 0.30 +0.2 2,602,031 - BT2077 0.30 -2.9 2,602,267 + BT2077 0.44 -4.4 2,602,268 - BT2077 0.44 -1.7 2,602,268 - BT2077 0.44 -3.6 2,602,279 + BT2077 0.45 -3.5 2,602,333 - BT2077 0.48 -1.8 2,602,368 + BT2077 0.50 -2.8 2,602,385 - BT2077 0.51 +0.8 2,602,398 + BT2077 0.52 -1.7 2,602,401 + BT2077 0.52 -2.8 2,602,402 - BT2077 0.52 -1.1 2,602,402 - BT2077 0.52 -1.4 2,602,491 + BT2077 0.58 -5.0
Or see this region's nucleotide sequence