Experiment: Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse2; 10 generations
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS08715 and MMP_RS08720 are separated by 29 nucleotides MMP_RS08720 and MMP_RS09070 are separated by 89 nucleotides MMP_RS09070 and MMP_RS08725 are separated by 15 nucleotides
MMP_RS08715: MMP_RS08715 - formylmethanofuran dehydrogenase subunit B, at 1,631,371 to 1,632,675
_RS08715
MMP_RS08720: MMP_RS08720 - 4Fe-4S binding protein, at 1,632,705 to 1,633,892
_RS08720
MMP_RS09070: MMP_RS09070 - F420 non-reducing hydrogenase subunit, at 1,633,982 to 1,634,116
_RS09070
MMP_RS08725: MMP_RS08725 - Ni/Fe hydrogenase subunit alpha, at 1,634,132 to 1,635,388
_RS08725
Position (kb)
1632
1633
1634 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1631.827 kb on + strand, within MMP_RS08715 at 1631.829 kb on - strand, within MMP_RS08715 at 1632.471 kb on + strand, within MMP_RS08715 at 1632.652 kb on - strand at 1632.782 kb on - strand at 1633.411 kb on + strand, within MMP_RS08720 at 1633.412 kb on - strand, within MMP_RS08720 at 1633.668 kb on - strand, within MMP_RS08720 at 1633.746 kb on - strand, within MMP_RS08720 at 1634.354 kb on - strand, within MMP_RS08725 at 1634.721 kb on - strand, within MMP_RS08725
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse2; 10 generations remove 1,631,827 + MMP_RS08715 0.35 -2.8 1,631,829 - MMP_RS08715 0.35 -3.7 1,632,471 + MMP_RS08715 0.84 -4.7 1,632,652 - -3.3 1,632,782 - -3.5 1,633,411 + MMP_RS08720 0.59 -3.3 1,633,412 - MMP_RS08720 0.60 -2.3 1,633,668 - MMP_RS08720 0.81 -2.4 1,633,746 - MMP_RS08720 0.88 -4.4 1,634,354 - MMP_RS08725 0.18 +0.6 1,634,721 - MMP_RS08725 0.47 -3.8
Or see this region's nucleotide sequence