Experiment: Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse2; 10 generations
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01000 and MMP_RS01005 are separated by 174 nucleotides MMP_RS01005 and MMP_RS01010 are separated by 128 nucleotides MMP_RS01010 and MMP_RS01015 are separated by 250 nucleotides
MMP_RS01000: MMP_RS01000 - AIR synthase related protein, at 187,497 to 190,466
_RS01000
MMP_RS01005: MMP_RS01005 - riboflavin synthase, at 190,641 to 191,111
_RS01005
MMP_RS01010: MMP_RS01010 - DUF4352 domain-containing protein, at 191,240 to 191,773
_RS01010
MMP_RS01015: MMP_RS01015 - hypothetical protein, at 192,024 to 192,236
_RS01015
Position (kb) 190
191
192 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 189.906 kb on - strand at 190.090 kb on + strand at 190.542 kb on - strand at 190.547 kb on - strand at 190.549 kb on - strand at 190.579 kb on - strand at 190.589 kb on + strand at 190.627 kb on + strand at 190.729 kb on - strand, within MMP_RS01005 at 190.730 kb on + strand, within MMP_RS01005 at 191.097 kb on - strand at 191.144 kb on - strand at 191.151 kb on - strand at 191.152 kb on + strand at 191.186 kb on - strand at 191.191 kb on - strand at 191.273 kb on - strand at 191.273 kb on - strand at 191.274 kb on + strand at 191.280 kb on - strand at 191.281 kb on + strand at 191.303 kb on + strand, within MMP_RS01010 at 191.309 kb on - strand, within MMP_RS01010 at 191.396 kb on - strand, within MMP_RS01010 at 191.397 kb on + strand, within MMP_RS01010 at 191.397 kb on + strand, within MMP_RS01010 at 191.442 kb on + strand, within MMP_RS01010 at 191.478 kb on + strand, within MMP_RS01010 at 191.519 kb on + strand, within MMP_RS01010 at 191.522 kb on - strand, within MMP_RS01010 at 191.523 kb on + strand, within MMP_RS01010 at 191.572 kb on - strand, within MMP_RS01010 at 191.572 kb on - strand, within MMP_RS01010 at 191.715 kb on + strand, within MMP_RS01010 at 191.740 kb on - strand at 191.750 kb on - strand at 191.750 kb on - strand at 191.753 kb on - strand at 191.754 kb on + strand at 191.754 kb on + strand at 191.754 kb on + strand at 191.755 kb on - strand at 191.756 kb on + strand at 191.779 kb on - strand at 191.780 kb on + strand at 191.781 kb on - strand at 191.795 kb on - strand at 191.795 kb on - strand at 191.795 kb on - strand at 191.801 kb on + strand at 191.801 kb on + strand at 191.841 kb on + strand at 191.852 kb on + strand at 191.854 kb on - strand at 191.855 kb on + strand at 191.871 kb on + strand at 191.874 kb on - strand at 191.875 kb on + strand at 191.912 kb on - strand at 191.993 kb on - strand at 191.994 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse2; 10 generations remove 189,906 - -2.0 190,090 + -2.5 190,542 - -1.7 190,547 - +0.1 190,549 - -1.2 190,579 - +0.5 190,589 + +0.6 190,627 + +0.5 190,729 - MMP_RS01005 0.19 -4.6 190,730 + MMP_RS01005 0.19 -5.3 191,097 - -6.1 191,144 - -4.2 191,151 - -2.7 191,152 + -4.6 191,186 - +0.8 191,191 - +0.2 191,273 - +0.7 191,273 - -0.2 191,274 + -0.6 191,280 - -1.3 191,281 + +0.6 191,303 + MMP_RS01010 0.12 +0.4 191,309 - MMP_RS01010 0.13 -0.3 191,396 - MMP_RS01010 0.29 +0.3 191,397 + MMP_RS01010 0.29 +0.4 191,397 + MMP_RS01010 0.29 +0.4 191,442 + MMP_RS01010 0.38 +0.4 191,478 + MMP_RS01010 0.45 +0.4 191,519 + MMP_RS01010 0.52 +0.4 191,522 - MMP_RS01010 0.53 -0.2 191,523 + MMP_RS01010 0.53 +0.0 191,572 - MMP_RS01010 0.62 -0.8 191,572 - MMP_RS01010 0.62 -0.1 191,715 + MMP_RS01010 0.89 -0.3 191,740 - -1.0 191,750 - +0.2 191,750 - +1.0 191,753 - +0.1 191,754 + +0.5 191,754 + +0.2 191,754 + -1.2 191,755 - +0.0 191,756 + +0.3 191,779 - +0.2 191,780 + +0.3 191,781 - -0.0 191,795 - +0.1 191,795 - -0.4 191,795 - +0.8 191,801 + +0.1 191,801 + +1.3 191,841 + +0.5 191,852 + -0.1 191,854 - -0.8 191,855 + +0.0 191,871 + -0.2 191,874 - +0.7 191,875 + +0.2 191,912 - +0.3 191,993 - -0.1 191,994 + +0.4
Or see this region's nucleotide sequence