Experiment: L-Serine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS24735 and EX31_RS24740 are separated by 250 nucleotides EX31_RS24740 and EX31_RS24745 are separated by 161 nucleotides EX31_RS24745 and EX31_RS24750 are separated by 131 nucleotides
EX31_RS24735: EX31_RS24735 - formate-dependent phosphoribosylglycinamide formyltransferase, at 716,691 to 717,869
_RS24735
EX31_RS24740: EX31_RS24740 - ATP-dependent RNA helicase DbpA, at 718,120 to 719,502
_RS24740
EX31_RS24745: EX31_RS24745 - hypothetical protein, at 719,664 to 720,029
_RS24745
EX31_RS24750: EX31_RS24750 - prolyl oligopeptidase family serine peptidase, at 720,161 to 722,200
_RS24750
Position (kb) 718
719
720 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 717.257 kb on + strand, within EX31_RS24735 at 717.332 kb on - strand, within EX31_RS24735 at 717.332 kb on - strand, within EX31_RS24735 at 717.476 kb on - strand, within EX31_RS24735 at 717.689 kb on + strand, within EX31_RS24735 at 717.692 kb on + strand, within EX31_RS24735 at 717.692 kb on + strand, within EX31_RS24735 at 717.693 kb on - strand, within EX31_RS24735 at 717.693 kb on - strand, within EX31_RS24735 at 717.740 kb on - strand, within EX31_RS24735 at 717.816 kb on + strand at 717.816 kb on + strand at 717.817 kb on - strand at 717.817 kb on - strand at 717.820 kb on - strand at 717.820 kb on - strand at 717.820 kb on - strand at 717.854 kb on + strand at 717.864 kb on + strand at 717.869 kb on + strand at 717.869 kb on + strand at 717.870 kb on - strand at 717.870 kb on - strand at 717.920 kb on + strand at 717.920 kb on + strand at 717.920 kb on + strand at 717.920 kb on + strand at 717.920 kb on + strand at 717.920 kb on + strand at 717.920 kb on + strand at 717.920 kb on + strand at 717.920 kb on + strand at 717.921 kb on - strand at 717.921 kb on - strand at 717.921 kb on - strand at 717.921 kb on - strand at 717.921 kb on - strand at 717.921 kb on - strand at 717.921 kb on - strand at 717.921 kb on - strand at 717.921 kb on - strand at 717.922 kb on + strand at 717.923 kb on - strand at 717.933 kb on - strand at 717.973 kb on + strand at 718.105 kb on - strand at 718.105 kb on - strand at 718.105 kb on - strand at 718.112 kb on + strand at 718.112 kb on + strand at 718.189 kb on + strand at 718.278 kb on + strand, within EX31_RS24740 at 718.349 kb on + strand, within EX31_RS24740 at 718.349 kb on + strand, within EX31_RS24740 at 718.394 kb on + strand, within EX31_RS24740 at 718.395 kb on - strand, within EX31_RS24740 at 718.519 kb on - strand, within EX31_RS24740 at 718.568 kb on + strand, within EX31_RS24740 at 718.568 kb on + strand, within EX31_RS24740 at 718.568 kb on + strand, within EX31_RS24740 at 718.569 kb on - strand, within EX31_RS24740 at 718.569 kb on - strand, within EX31_RS24740 at 718.569 kb on - strand, within EX31_RS24740 at 718.620 kb on + strand, within EX31_RS24740 at 718.639 kb on + strand, within EX31_RS24740 at 718.641 kb on + strand, within EX31_RS24740 at 718.641 kb on + strand, within EX31_RS24740 at 718.642 kb on - strand, within EX31_RS24740 at 718.736 kb on + strand, within EX31_RS24740 at 718.767 kb on + strand, within EX31_RS24740 at 718.767 kb on + strand, within EX31_RS24740 at 718.831 kb on + strand, within EX31_RS24740 at 718.831 kb on + strand, within EX31_RS24740 at 718.832 kb on - strand, within EX31_RS24740 at 718.837 kb on + strand, within EX31_RS24740 at 718.866 kb on + strand, within EX31_RS24740 at 719.043 kb on + strand, within EX31_RS24740 at 719.044 kb on - strand, within EX31_RS24740 at 719.044 kb on - strand, within EX31_RS24740 at 719.064 kb on + strand, within EX31_RS24740 at 719.068 kb on + strand, within EX31_RS24740 at 719.069 kb on - strand, within EX31_RS24740 at 719.069 kb on - strand, within EX31_RS24740 at 719.197 kb on + strand, within EX31_RS24740 at 719.198 kb on - strand, within EX31_RS24740 at 719.198 kb on - strand, within EX31_RS24740 at 719.214 kb on + strand, within EX31_RS24740 at 719.283 kb on - strand, within EX31_RS24740 at 719.385 kb on + strand at 719.404 kb on + strand at 719.404 kb on + strand at 719.404 kb on + strand at 719.404 kb on + strand at 719.404 kb on + strand at 719.404 kb on + strand at 719.404 kb on + strand at 719.405 kb on - strand at 719.405 kb on - strand at 719.467 kb on - strand at 719.491 kb on - strand at 719.572 kb on + strand at 719.576 kb on - strand at 719.602 kb on + strand at 719.602 kb on + strand at 719.603 kb on - strand at 719.603 kb on - strand at 719.606 kb on + strand at 719.613 kb on - strand at 719.855 kb on + strand, within EX31_RS24745 at 719.855 kb on + strand, within EX31_RS24745 at 719.855 kb on + strand, within EX31_RS24745 at 719.933 kb on + strand, within EX31_RS24745 at 719.966 kb on + strand, within EX31_RS24745 at 719.966 kb on + strand, within EX31_RS24745 at 719.967 kb on - strand, within EX31_RS24745 at 720.012 kb on + strand at 720.012 kb on + strand at 720.013 kb on - strand at 720.025 kb on + strand at 720.077 kb on - strand at 720.208 kb on - strand at 720.208 kb on - strand at 720.208 kb on - strand at 720.229 kb on + strand at 720.230 kb on - strand at 720.233 kb on + strand at 720.233 kb on + strand at 720.234 kb on - strand at 720.281 kb on + strand at 720.302 kb on + strand at 720.303 kb on - strand at 720.304 kb on + strand at 720.305 kb on - strand at 720.305 kb on - strand at 720.350 kb on + strand at 720.370 kb on + strand, within EX31_RS24750 at 720.398 kb on + strand, within EX31_RS24750 at 720.399 kb on - strand, within EX31_RS24750 at 720.458 kb on + strand, within EX31_RS24750 at 720.459 kb on - strand, within EX31_RS24750
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); ShewMM media; no shaking remove 717,257 + EX31_RS24735 0.48 -0.6 717,332 - EX31_RS24735 0.54 +0.7 717,332 - EX31_RS24735 0.54 +0.8 717,476 - EX31_RS24735 0.67 +0.2 717,689 + EX31_RS24735 0.85 -0.6 717,692 + EX31_RS24735 0.85 +0.1 717,692 + EX31_RS24735 0.85 -1.3 717,693 - EX31_RS24735 0.85 -0.7 717,693 - EX31_RS24735 0.85 +0.3 717,740 - EX31_RS24735 0.89 +1.0 717,816 + +0.6 717,816 + -0.3 717,817 - -2.5 717,817 - +0.5 717,820 - -0.8 717,820 - +1.9 717,820 - -0.4 717,854 + -0.2 717,864 + -0.1 717,869 + +0.1 717,869 + +0.1 717,870 - -0.6 717,870 - -0.1 717,920 + +0.7 717,920 + +1.2 717,920 + -0.6 717,920 + +1.0 717,920 + -1.5 717,920 + +0.5 717,920 + +0.3 717,920 + +1.0 717,920 + +0.4 717,921 - -2.1 717,921 - -1.1 717,921 - -0.6 717,921 - +1.7 717,921 - -1.7 717,921 - -0.1 717,921 - +0.7 717,921 - +1.1 717,921 - +0.3 717,922 + +0.8 717,923 - -0.0 717,933 - +2.0 717,973 + +0.7 718,105 - +1.1 718,105 - -0.6 718,105 - -1.2 718,112 + +1.7 718,112 + -1.6 718,189 + +0.4 718,278 + EX31_RS24740 0.11 +0.5 718,349 + EX31_RS24740 0.17 -0.2 718,349 + EX31_RS24740 0.17 +2.1 718,394 + EX31_RS24740 0.20 -0.3 718,395 - EX31_RS24740 0.20 -0.5 718,519 - EX31_RS24740 0.29 +0.1 718,568 + EX31_RS24740 0.32 +1.7 718,568 + EX31_RS24740 0.32 +1.2 718,568 + EX31_RS24740 0.32 +0.9 718,569 - EX31_RS24740 0.32 +1.0 718,569 - EX31_RS24740 0.32 -0.0 718,569 - EX31_RS24740 0.32 -2.0 718,620 + EX31_RS24740 0.36 +1.0 718,639 + EX31_RS24740 0.38 +0.7 718,641 + EX31_RS24740 0.38 +1.3 718,641 + EX31_RS24740 0.38 +1.3 718,642 - EX31_RS24740 0.38 +0.4 718,736 + EX31_RS24740 0.45 +0.1 718,767 + EX31_RS24740 0.47 +0.5 718,767 + EX31_RS24740 0.47 +0.2 718,831 + EX31_RS24740 0.51 +1.2 718,831 + EX31_RS24740 0.51 -0.3 718,832 - EX31_RS24740 0.51 -0.1 718,837 + EX31_RS24740 0.52 -0.3 718,866 + EX31_RS24740 0.54 +1.3 719,043 + EX31_RS24740 0.67 +0.8 719,044 - EX31_RS24740 0.67 +0.3 719,044 - EX31_RS24740 0.67 +1.9 719,064 + EX31_RS24740 0.68 -0.4 719,068 + EX31_RS24740 0.69 +0.4 719,069 - EX31_RS24740 0.69 +1.0 719,069 - EX31_RS24740 0.69 +3.3 719,197 + EX31_RS24740 0.78 -1.1 719,198 - EX31_RS24740 0.78 +0.1 719,198 - EX31_RS24740 0.78 +1.7 719,214 + EX31_RS24740 0.79 -0.6 719,283 - EX31_RS24740 0.84 +0.3 719,385 + -0.0 719,404 + +0.8 719,404 + +1.2 719,404 + +0.6 719,404 + +0.8 719,404 + -2.1 719,404 + -0.7 719,404 + +1.3 719,405 - -0.1 719,405 - -0.8 719,467 - +0.1 719,491 - -0.5 719,572 + -1.8 719,576 - +0.6 719,602 + +0.2 719,602 + +0.5 719,603 - -0.0 719,603 - +1.7 719,606 + +0.4 719,613 - -0.9 719,855 + EX31_RS24745 0.52 +1.1 719,855 + EX31_RS24745 0.52 +0.0 719,855 + EX31_RS24745 0.52 +1.6 719,933 + EX31_RS24745 0.73 +0.4 719,966 + EX31_RS24745 0.83 +0.5 719,966 + EX31_RS24745 0.83 -0.3 719,967 - EX31_RS24745 0.83 -0.2 720,012 + +0.8 720,012 + -0.2 720,013 - +1.3 720,025 + +0.5 720,077 - +0.7 720,208 - -1.1 720,208 - +0.1 720,208 - +1.4 720,229 + -0.8 720,230 - +1.7 720,233 + -0.3 720,233 + +0.6 720,234 - +0.1 720,281 + +0.5 720,302 + -0.3 720,303 - -0.3 720,304 + +0.1 720,305 - +3.1 720,305 - -0.6 720,350 + -0.1 720,370 + EX31_RS24750 0.10 -0.3 720,398 + EX31_RS24750 0.12 +0.4 720,399 - EX31_RS24750 0.12 +1.4 720,458 + EX31_RS24750 0.15 -0.3 720,459 - EX31_RS24750 0.15 -1.6
Or see this region's nucleotide sequence