Experiment: L-Serine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS23410 and EX31_RS23415 are separated by 285 nucleotides EX31_RS23415 and EX31_RS23420 are separated by 827 nucleotides
EX31_RS23410: EX31_RS23410 - autotransporter outer membrane beta-barrel domain-containing protein, at 445,439 to 448,342
_RS23410
EX31_RS23415: EX31_RS23415 - citrate synthase, at 448,628 to 449,911
_RS23415
EX31_RS23420: EX31_RS23420 - succinate dehydrogenase cytochrome b556 subunit, at 450,739 to 451,128
_RS23420
Position (kb) 448
449
450 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 447.629 kb on + strand, within EX31_RS23410 at 447.630 kb on - strand, within EX31_RS23410 at 447.632 kb on + strand, within EX31_RS23410 at 447.632 kb on + strand, within EX31_RS23410 at 447.633 kb on - strand, within EX31_RS23410 at 447.675 kb on + strand, within EX31_RS23410 at 447.676 kb on - strand, within EX31_RS23410 at 447.676 kb on - strand, within EX31_RS23410 at 447.676 kb on - strand, within EX31_RS23410 at 447.676 kb on - strand, within EX31_RS23410 at 447.676 kb on - strand, within EX31_RS23410 at 447.773 kb on + strand, within EX31_RS23410 at 447.774 kb on - strand, within EX31_RS23410 at 447.779 kb on + strand, within EX31_RS23410 at 447.785 kb on + strand, within EX31_RS23410 at 447.785 kb on + strand, within EX31_RS23410 at 447.786 kb on - strand, within EX31_RS23410 at 447.861 kb on - strand, within EX31_RS23410 at 447.861 kb on - strand, within EX31_RS23410 at 447.861 kb on - strand, within EX31_RS23410 at 447.861 kb on - strand, within EX31_RS23410 at 447.862 kb on + strand, within EX31_RS23410 at 447.862 kb on + strand, within EX31_RS23410 at 447.863 kb on - strand, within EX31_RS23410 at 447.900 kb on + strand, within EX31_RS23410 at 447.901 kb on - strand, within EX31_RS23410 at 447.944 kb on - strand, within EX31_RS23410 at 448.110 kb on - strand at 448.170 kb on + strand at 448.170 kb on + strand at 448.171 kb on - strand at 448.171 kb on - strand at 448.196 kb on - strand at 448.223 kb on + strand at 448.258 kb on + strand at 448.258 kb on + strand at 448.258 kb on + strand at 448.445 kb on - strand at 448.545 kb on - strand at 448.549 kb on - strand at 448.552 kb on + strand at 448.572 kb on + strand at 448.609 kb on - strand at 448.702 kb on + strand at 448.895 kb on + strand, within EX31_RS23415 at 448.986 kb on + strand, within EX31_RS23415 at 448.987 kb on - strand, within EX31_RS23415 at 449.246 kb on - strand, within EX31_RS23415 at 449.246 kb on - strand, within EX31_RS23415 at 449.331 kb on + strand, within EX31_RS23415 at 449.331 kb on + strand, within EX31_RS23415 at 449.331 kb on + strand, within EX31_RS23415 at 449.331 kb on + strand, within EX31_RS23415 at 449.331 kb on + strand, within EX31_RS23415 at 449.331 kb on + strand, within EX31_RS23415 at 449.331 kb on + strand, within EX31_RS23415 at 449.332 kb on - strand, within EX31_RS23415 at 449.352 kb on + strand, within EX31_RS23415 at 449.354 kb on + strand, within EX31_RS23415 at 449.354 kb on + strand, within EX31_RS23415 at 449.355 kb on - strand, within EX31_RS23415 at 449.355 kb on - strand, within EX31_RS23415 at 449.355 kb on - strand, within EX31_RS23415 at 449.376 kb on + strand, within EX31_RS23415 at 449.376 kb on + strand, within EX31_RS23415 at 449.383 kb on - strand, within EX31_RS23415 at 449.410 kb on + strand, within EX31_RS23415 at 449.425 kb on - strand, within EX31_RS23415 at 449.457 kb on - strand, within EX31_RS23415 at 449.457 kb on - strand, within EX31_RS23415 at 449.476 kb on - strand, within EX31_RS23415 at 449.610 kb on + strand, within EX31_RS23415 at 449.649 kb on + strand, within EX31_RS23415 at 449.649 kb on + strand, within EX31_RS23415 at 449.649 kb on + strand, within EX31_RS23415 at 449.650 kb on - strand, within EX31_RS23415 at 449.650 kb on - strand, within EX31_RS23415 at 449.650 kb on - strand, within EX31_RS23415 at 449.734 kb on + strand, within EX31_RS23415 at 449.736 kb on + strand, within EX31_RS23415 at 449.736 kb on + strand, within EX31_RS23415 at 449.737 kb on - strand, within EX31_RS23415 at 449.737 kb on - strand, within EX31_RS23415 at 449.737 kb on - strand, within EX31_RS23415 at 449.764 kb on + strand, within EX31_RS23415 at 449.785 kb on - strand at 449.888 kb on - strand at 449.980 kb on + strand at 449.980 kb on + strand at 449.980 kb on + strand at 449.980 kb on + strand at 449.980 kb on + strand at 449.980 kb on + strand at 449.981 kb on - strand at 449.981 kb on - strand at 449.981 kb on - strand at 449.981 kb on - strand at 449.981 kb on - strand at 449.981 kb on - strand at 449.989 kb on + strand at 449.990 kb on - strand at 449.992 kb on + strand at 449.993 kb on - strand at 449.993 kb on - strand at 450.005 kb on - strand at 450.005 kb on - strand at 450.033 kb on + strand at 450.033 kb on + strand at 450.034 kb on - strand at 450.116 kb on + strand at 450.117 kb on - strand at 450.135 kb on - strand at 450.147 kb on + strand at 450.274 kb on + strand at 450.274 kb on + strand at 450.275 kb on - strand at 450.275 kb on - strand at 450.296 kb on + strand at 450.297 kb on - strand at 450.297 kb on - strand at 450.297 kb on - strand at 450.297 kb on - strand at 450.297 kb on - strand at 450.304 kb on + strand at 450.304 kb on + strand at 450.305 kb on - strand at 450.382 kb on + strand at 450.382 kb on + strand at 450.383 kb on - strand at 450.383 kb on - strand at 450.383 kb on - strand at 450.383 kb on - strand at 450.383 kb on - strand at 450.451 kb on + strand at 450.451 kb on + strand at 450.453 kb on + strand at 450.459 kb on + strand at 450.459 kb on + strand at 450.484 kb on + strand at 450.485 kb on - strand at 450.535 kb on + strand at 450.562 kb on + strand at 450.609 kb on + strand at 450.609 kb on + strand at 450.609 kb on + strand at 450.702 kb on + strand at 450.702 kb on + strand at 450.702 kb on + strand at 450.702 kb on + strand at 450.702 kb on + strand at 450.702 kb on + strand at 450.703 kb on - strand at 450.824 kb on + strand, within EX31_RS23420 at 450.825 kb on - strand, within EX31_RS23420 at 450.846 kb on + strand, within EX31_RS23420 at 450.846 kb on + strand, within EX31_RS23420 at 450.846 kb on + strand, within EX31_RS23420 at 450.846 kb on + strand, within EX31_RS23420 at 450.846 kb on + strand, within EX31_RS23420 at 450.846 kb on + strand, within EX31_RS23420
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); ShewMM media; no shaking remove 447,629 + EX31_RS23410 0.75 +0.2 447,630 - EX31_RS23410 0.75 -0.1 447,632 + EX31_RS23410 0.76 -0.1 447,632 + EX31_RS23410 0.76 +0.9 447,633 - EX31_RS23410 0.76 -0.1 447,675 + EX31_RS23410 0.77 -0.0 447,676 - EX31_RS23410 0.77 +0.0 447,676 - EX31_RS23410 0.77 +1.2 447,676 - EX31_RS23410 0.77 -1.2 447,676 - EX31_RS23410 0.77 -1.4 447,676 - EX31_RS23410 0.77 -0.3 447,773 + EX31_RS23410 0.80 +1.1 447,774 - EX31_RS23410 0.80 +1.1 447,779 + EX31_RS23410 0.81 +0.8 447,785 + EX31_RS23410 0.81 +0.6 447,785 + EX31_RS23410 0.81 +0.9 447,786 - EX31_RS23410 0.81 +1.4 447,861 - EX31_RS23410 0.83 -0.8 447,861 - EX31_RS23410 0.83 -1.6 447,861 - EX31_RS23410 0.83 +4.1 447,861 - EX31_RS23410 0.83 +1.2 447,862 + EX31_RS23410 0.83 +1.8 447,862 + EX31_RS23410 0.83 -0.9 447,863 - EX31_RS23410 0.83 +0.9 447,900 + EX31_RS23410 0.85 +0.3 447,901 - EX31_RS23410 0.85 +0.7 447,944 - EX31_RS23410 0.86 -0.7 448,110 - +0.8 448,170 + -0.3 448,170 + +0.2 448,171 - +1.8 448,171 - -0.2 448,196 - +1.6 448,223 + +0.9 448,258 + +1.5 448,258 + -0.6 448,258 + -0.1 448,445 - -1.2 448,545 - -0.7 448,549 - -1.2 448,552 + -1.3 448,572 + +0.4 448,609 - +0.6 448,702 + -0.6 448,895 + EX31_RS23415 0.21 -2.1 448,986 + EX31_RS23415 0.28 -3.0 448,987 - EX31_RS23415 0.28 -1.9 449,246 - EX31_RS23415 0.48 -3.7 449,246 - EX31_RS23415 0.48 -2.9 449,331 + EX31_RS23415 0.55 -2.7 449,331 + EX31_RS23415 0.55 -1.7 449,331 + EX31_RS23415 0.55 +1.8 449,331 + EX31_RS23415 0.55 -2.7 449,331 + EX31_RS23415 0.55 -1.6 449,331 + EX31_RS23415 0.55 -3.1 449,331 + EX31_RS23415 0.55 -1.9 449,332 - EX31_RS23415 0.55 -3.1 449,352 + EX31_RS23415 0.56 -3.6 449,354 + EX31_RS23415 0.57 -3.2 449,354 + EX31_RS23415 0.57 -2.2 449,355 - EX31_RS23415 0.57 -3.0 449,355 - EX31_RS23415 0.57 -1.9 449,355 - EX31_RS23415 0.57 -2.6 449,376 + EX31_RS23415 0.58 -2.1 449,376 + EX31_RS23415 0.58 -2.2 449,383 - EX31_RS23415 0.59 -1.2 449,410 + EX31_RS23415 0.61 -3.1 449,425 - EX31_RS23415 0.62 -2.0 449,457 - EX31_RS23415 0.65 -3.3 449,457 - EX31_RS23415 0.65 -3.1 449,476 - EX31_RS23415 0.66 -1.6 449,610 + EX31_RS23415 0.76 -1.4 449,649 + EX31_RS23415 0.80 -2.0 449,649 + EX31_RS23415 0.80 -1.9 449,649 + EX31_RS23415 0.80 -2.0 449,650 - EX31_RS23415 0.80 -3.0 449,650 - EX31_RS23415 0.80 -1.4 449,650 - EX31_RS23415 0.80 -2.6 449,734 + EX31_RS23415 0.86 -1.4 449,736 + EX31_RS23415 0.86 -1.9 449,736 + EX31_RS23415 0.86 -3.1 449,737 - EX31_RS23415 0.86 -1.8 449,737 - EX31_RS23415 0.86 -1.6 449,737 - EX31_RS23415 0.86 -1.8 449,764 + EX31_RS23415 0.88 -0.6 449,785 - -0.4 449,888 - -3.2 449,980 + -2.2 449,980 + -3.0 449,980 + -2.2 449,980 + -2.9 449,980 + -2.2 449,980 + -1.7 449,981 - -0.0 449,981 - +0.4 449,981 - -0.0 449,981 - +0.4 449,981 - -0.9 449,981 - +0.3 449,989 + -2.6 449,990 - -1.2 449,992 + -2.5 449,993 - +0.2 449,993 - +0.0 450,005 - -0.3 450,005 - +1.8 450,033 + -3.0 450,033 + -0.2 450,034 - -1.0 450,116 + -2.2 450,117 - -0.1 450,135 - -1.4 450,147 + -2.9 450,274 + +1.1 450,274 + +0.4 450,275 - -1.2 450,275 - -0.7 450,296 + -0.1 450,297 - -0.7 450,297 - -0.8 450,297 - -0.7 450,297 - +0.2 450,297 - -1.9 450,304 + +0.3 450,304 + +1.3 450,305 - -0.8 450,382 + -1.4 450,382 + +1.6 450,383 - +0.5 450,383 - +1.1 450,383 - -0.2 450,383 - -0.3 450,383 - +1.0 450,451 + +0.5 450,451 + -0.1 450,453 + +0.8 450,459 + +0.2 450,459 + +1.3 450,484 + -0.2 450,485 - +0.2 450,535 + -0.6 450,562 + -0.2 450,609 + +1.4 450,609 + -0.7 450,609 + +0.9 450,702 + +0.8 450,702 + +0.1 450,702 + -1.2 450,702 + +0.8 450,702 + +1.1 450,702 + -0.7 450,703 - +0.2 450,824 + EX31_RS23420 0.22 +1.3 450,825 - EX31_RS23420 0.22 -1.1 450,846 + EX31_RS23420 0.27 -0.4 450,846 + EX31_RS23420 0.27 +0.3 450,846 + EX31_RS23420 0.27 -0.2 450,846 + EX31_RS23420 0.27 +0.8 450,846 + EX31_RS23420 0.27 +0.1 450,846 + EX31_RS23420 0.27 -1.7
Or see this region's nucleotide sequence