Experiment: L-Serine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS22160 and EX31_RS22165 are separated by 5 nucleotides EX31_RS22165 and EX31_RS22170 are separated by 276 nucleotides EX31_RS22170 and EX31_RS22175 are separated by 66 nucleotides EX31_RS22175 and EX31_RS22180 overlap by 38 nucleotides
EX31_RS22160: EX31_RS22160 - (2E,6E)-farnesyl diphosphate synthase, at 180,530 to 181,459
_RS22160
EX31_RS22165: EX31_RS22165 - exodeoxyribonuclease VII small subunit, at 181,465 to 181,707
_RS22165
EX31_RS22170: EX31_RS22170 - tRNA 4-thiouridine(8) synthase ThiI, at 181,984 to 183,432
_RS22170
EX31_RS22175: EX31_RS22175 - protein deglycase YajL, at 183,499 to 184,107
_RS22175
EX31_RS22180: EX31_RS22180 - 2-dehydropantoate 2-reductase, at 184,070 to 184,981
_RS22180
Position (kb) 181
182
183
184 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 181.708 kb on - strand at 181.719 kb on - strand at 181.719 kb on - strand at 181.719 kb on - strand at 181.719 kb on - strand at 181.750 kb on - strand at 181.750 kb on - strand at 181.842 kb on - strand at 181.848 kb on + strand at 181.848 kb on + strand at 181.848 kb on + strand at 181.848 kb on + strand at 181.849 kb on - strand at 181.849 kb on - strand at 181.849 kb on - strand at 181.849 kb on - strand at 181.851 kb on - strand at 181.851 kb on - strand at 181.851 kb on - strand at 181.851 kb on - strand at 181.851 kb on - strand at 181.851 kb on - strand at 181.851 kb on - strand at 181.851 kb on - strand at 181.851 kb on - strand at 181.851 kb on - strand at 181.851 kb on - strand at 181.851 kb on - strand at 181.851 kb on - strand at 181.867 kb on - strand at 181.867 kb on - strand at 181.870 kb on - strand at 181.870 kb on - strand at 181.876 kb on - strand at 181.876 kb on - strand at 181.915 kb on + strand at 181.916 kb on - strand at 181.916 kb on - strand at 181.916 kb on - strand at 181.918 kb on + strand at 181.918 kb on + strand at 181.919 kb on - strand at 181.926 kb on + strand at 181.926 kb on + strand at 181.926 kb on + strand at 181.927 kb on - strand at 181.935 kb on - strand at 181.946 kb on + strand at 181.946 kb on + strand at 181.946 kb on + strand at 181.947 kb on - strand at 181.947 kb on - strand at 181.947 kb on - strand at 181.947 kb on - strand at 181.947 kb on - strand at 181.950 kb on - strand at 181.969 kb on + strand at 181.969 kb on + strand at 181.992 kb on + strand at 181.992 kb on + strand at 181.993 kb on - strand at 181.998 kb on + strand at 182.052 kb on + strand at 182.053 kb on - strand at 182.053 kb on - strand at 182.064 kb on + strand at 182.064 kb on + strand at 182.064 kb on + strand at 182.064 kb on + strand at 182.064 kb on + strand at 182.064 kb on + strand at 182.145 kb on + strand, within EX31_RS22170 at 182.145 kb on + strand, within EX31_RS22170 at 182.145 kb on + strand, within EX31_RS22170 at 182.206 kb on + strand, within EX31_RS22170 at 182.328 kb on + strand, within EX31_RS22170 at 182.354 kb on + strand, within EX31_RS22170 at 182.354 kb on + strand, within EX31_RS22170 at 182.355 kb on - strand, within EX31_RS22170 at 182.362 kb on + strand, within EX31_RS22170 at 182.362 kb on + strand, within EX31_RS22170 at 182.362 kb on + strand, within EX31_RS22170 at 182.362 kb on + strand, within EX31_RS22170 at 182.363 kb on - strand, within EX31_RS22170 at 182.455 kb on - strand, within EX31_RS22170 at 182.566 kb on + strand, within EX31_RS22170 at 182.566 kb on + strand, within EX31_RS22170 at 182.566 kb on + strand, within EX31_RS22170 at 182.566 kb on + strand, within EX31_RS22170 at 182.567 kb on - strand, within EX31_RS22170 at 182.568 kb on + strand, within EX31_RS22170 at 182.568 kb on + strand, within EX31_RS22170 at 182.568 kb on + strand, within EX31_RS22170 at 182.568 kb on + strand, within EX31_RS22170 at 182.568 kb on + strand, within EX31_RS22170 at 182.569 kb on - strand, within EX31_RS22170 at 182.599 kb on + strand, within EX31_RS22170 at 182.659 kb on + strand, within EX31_RS22170 at 182.659 kb on + strand, within EX31_RS22170 at 182.700 kb on + strand, within EX31_RS22170 at 182.781 kb on + strand, within EX31_RS22170 at 182.781 kb on + strand, within EX31_RS22170 at 182.781 kb on + strand, within EX31_RS22170 at 182.782 kb on - strand, within EX31_RS22170 at 182.782 kb on - strand, within EX31_RS22170 at 182.916 kb on + strand, within EX31_RS22170 at 182.916 kb on + strand, within EX31_RS22170 at 182.916 kb on + strand, within EX31_RS22170 at 182.917 kb on - strand, within EX31_RS22170 at 182.943 kb on + strand, within EX31_RS22170 at 183.147 kb on + strand, within EX31_RS22170 at 183.148 kb on - strand, within EX31_RS22170 at 183.292 kb on + strand at 183.337 kb on + strand at 183.337 kb on + strand at 183.337 kb on + strand at 183.346 kb on + strand at 183.346 kb on + strand at 183.346 kb on + strand at 183.385 kb on + strand at 183.386 kb on - strand at 183.386 kb on - strand at 183.406 kb on - strand at 183.430 kb on + strand at 183.448 kb on + strand at 183.448 kb on + strand at 183.448 kb on + strand at 183.449 kb on - strand at 183.527 kb on + strand at 183.527 kb on + strand at 183.528 kb on - strand at 183.528 kb on - strand at 183.528 kb on - strand at 183.528 kb on - strand at 183.528 kb on - strand at 183.531 kb on + strand at 183.531 kb on + strand at 183.531 kb on + strand at 183.532 kb on - strand at 183.532 kb on - strand at 183.532 kb on - strand at 183.532 kb on - strand at 183.532 kb on - strand at 183.533 kb on + strand at 183.533 kb on + strand at 183.533 kb on + strand at 183.534 kb on - strand at 183.534 kb on - strand at 183.534 kb on - strand at 183.534 kb on - strand at 183.534 kb on - strand at 183.696 kb on + strand, within EX31_RS22175 at 183.697 kb on - strand, within EX31_RS22175 at 183.719 kb on - strand, within EX31_RS22175 at 183.738 kb on + strand, within EX31_RS22175 at 183.754 kb on + strand, within EX31_RS22175 at 183.764 kb on + strand, within EX31_RS22175 at 183.764 kb on + strand, within EX31_RS22175 at 183.796 kb on - strand, within EX31_RS22175 at 183.796 kb on - strand, within EX31_RS22175 at 183.796 kb on - strand, within EX31_RS22175 at 183.929 kb on - strand, within EX31_RS22175 at 183.929 kb on - strand, within EX31_RS22175 at 183.929 kb on - strand, within EX31_RS22175 at 183.929 kb on - strand, within EX31_RS22175 at 183.929 kb on - strand, within EX31_RS22175 at 183.929 kb on - strand, within EX31_RS22175 at 183.929 kb on - strand, within EX31_RS22175 at 183.929 kb on - strand, within EX31_RS22175 at 183.929 kb on - strand, within EX31_RS22175 at 183.952 kb on - strand, within EX31_RS22175 at 184.060 kb on + strand at 184.060 kb on + strand at 184.061 kb on - strand at 184.126 kb on + strand at 184.127 kb on - strand at 184.139 kb on + strand at 184.139 kb on + strand at 184.151 kb on - strand at 184.175 kb on - strand, within EX31_RS22180 at 184.194 kb on + strand, within EX31_RS22180 at 184.195 kb on - strand, within EX31_RS22180 at 184.195 kb on - strand, within EX31_RS22180 at 184.204 kb on + strand, within EX31_RS22180 at 184.204 kb on + strand, within EX31_RS22180 at 184.205 kb on - strand, within EX31_RS22180 at 184.205 kb on - strand, within EX31_RS22180 at 184.205 kb on - strand, within EX31_RS22180 at 184.205 kb on - strand, within EX31_RS22180 at 184.205 kb on - strand, within EX31_RS22180 at 184.205 kb on - strand, within EX31_RS22180 at 184.205 kb on - strand, within EX31_RS22180 at 184.206 kb on + strand, within EX31_RS22180 at 184.207 kb on - strand, within EX31_RS22180 at 184.207 kb on - strand, within EX31_RS22180 at 184.207 kb on - strand, within EX31_RS22180 at 184.207 kb on - strand, within EX31_RS22180 at 184.287 kb on + strand, within EX31_RS22180 at 184.383 kb on + strand, within EX31_RS22180 at 184.384 kb on - strand, within EX31_RS22180 at 184.413 kb on + strand, within EX31_RS22180 at 184.413 kb on + strand, within EX31_RS22180
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); ShewMM media; no shaking remove 181,708 - -1.7 181,719 - -0.3 181,719 - -0.3 181,719 - -0.4 181,719 - +0.5 181,750 - -0.7 181,750 - -0.5 181,842 - -0.3 181,848 + +1.2 181,848 + +1.7 181,848 + +3.2 181,848 + +0.2 181,849 - -0.3 181,849 - -0.3 181,849 - +0.9 181,849 - -0.5 181,851 - +0.1 181,851 - +0.6 181,851 - -0.2 181,851 - -0.8 181,851 - -0.0 181,851 - -0.2 181,851 - +0.2 181,851 - -1.6 181,851 - -0.9 181,851 - -0.5 181,851 - +1.3 181,851 - -1.0 181,851 - +0.7 181,867 - -0.2 181,867 - -0.7 181,870 - +0.6 181,870 - -1.0 181,876 - -0.6 181,876 - -1.5 181,915 + +0.0 181,916 - +0.1 181,916 - +1.1 181,916 - -0.4 181,918 + -0.1 181,918 + +0.2 181,919 - -0.5 181,926 + +0.9 181,926 + +1.1 181,926 + +1.0 181,927 - +0.3 181,935 - +0.3 181,946 + +1.0 181,946 + +0.3 181,946 + +0.9 181,947 - +0.3 181,947 - +0.4 181,947 - +1.9 181,947 - +0.0 181,947 - -1.3 181,950 - +0.9 181,969 + -0.1 181,969 + -0.4 181,992 + +0.6 181,992 + -1.0 181,993 - -2.1 181,998 + -0.6 182,052 + +0.4 182,053 - -0.1 182,053 - -1.9 182,064 + -0.7 182,064 + -0.0 182,064 + -0.0 182,064 + -1.9 182,064 + -1.2 182,064 + +0.6 182,145 + EX31_RS22170 0.11 +0.7 182,145 + EX31_RS22170 0.11 -2.0 182,145 + EX31_RS22170 0.11 -0.5 182,206 + EX31_RS22170 0.15 +1.7 182,328 + EX31_RS22170 0.24 -1.3 182,354 + EX31_RS22170 0.26 -0.3 182,354 + EX31_RS22170 0.26 +1.9 182,355 - EX31_RS22170 0.26 -0.5 182,362 + EX31_RS22170 0.26 +0.3 182,362 + EX31_RS22170 0.26 -0.5 182,362 + EX31_RS22170 0.26 -0.8 182,362 + EX31_RS22170 0.26 -1.1 182,363 - EX31_RS22170 0.26 -0.2 182,455 - EX31_RS22170 0.33 +0.3 182,566 + EX31_RS22170 0.40 +0.6 182,566 + EX31_RS22170 0.40 +0.9 182,566 + EX31_RS22170 0.40 +0.5 182,566 + EX31_RS22170 0.40 -0.3 182,567 - EX31_RS22170 0.40 +0.5 182,568 + EX31_RS22170 0.40 -1.5 182,568 + EX31_RS22170 0.40 -1.7 182,568 + EX31_RS22170 0.40 +0.5 182,568 + EX31_RS22170 0.40 -1.0 182,568 + EX31_RS22170 0.40 -1.1 182,569 - EX31_RS22170 0.40 -0.7 182,599 + EX31_RS22170 0.42 -1.5 182,659 + EX31_RS22170 0.47 +0.3 182,659 + EX31_RS22170 0.47 -0.0 182,700 + EX31_RS22170 0.49 -0.1 182,781 + EX31_RS22170 0.55 -1.3 182,781 + EX31_RS22170 0.55 -0.1 182,781 + EX31_RS22170 0.55 -0.9 182,782 - EX31_RS22170 0.55 -1.7 182,782 - EX31_RS22170 0.55 -0.8 182,916 + EX31_RS22170 0.64 +0.9 182,916 + EX31_RS22170 0.64 +0.4 182,916 + EX31_RS22170 0.64 +1.2 182,917 - EX31_RS22170 0.64 +0.1 182,943 + EX31_RS22170 0.66 +0.0 183,147 + EX31_RS22170 0.80 -0.1 183,148 - EX31_RS22170 0.80 -1.7 183,292 + +0.2 183,337 + +0.4 183,337 + +0.0 183,337 + +0.7 183,346 + -0.7 183,346 + +0.1 183,346 + +0.7 183,385 + -0.1 183,386 - -1.4 183,386 - +0.8 183,406 - -1.4 183,430 + +0.6 183,448 + -0.9 183,448 + +0.5 183,448 + +0.6 183,449 - +0.1 183,527 + -0.1 183,527 + +1.7 183,528 - +0.0 183,528 - -0.2 183,528 - +0.1 183,528 - +2.4 183,528 - -2.0 183,531 + -1.4 183,531 + -0.7 183,531 + +0.7 183,532 - +0.5 183,532 - +0.4 183,532 - +0.2 183,532 - +0.2 183,532 - -1.5 183,533 + +1.0 183,533 + +0.2 183,533 + +0.3 183,534 - -0.2 183,534 - +0.1 183,534 - +1.0 183,534 - +0.7 183,534 - -0.7 183,696 + EX31_RS22175 0.32 -0.1 183,697 - EX31_RS22175 0.33 +0.4 183,719 - EX31_RS22175 0.36 -1.0 183,738 + EX31_RS22175 0.39 +1.9 183,754 + EX31_RS22175 0.42 +0.3 183,764 + EX31_RS22175 0.44 -1.5 183,764 + EX31_RS22175 0.44 -0.2 183,796 - EX31_RS22175 0.49 +0.3 183,796 - EX31_RS22175 0.49 +0.8 183,796 - EX31_RS22175 0.49 +0.6 183,929 - EX31_RS22175 0.71 +0.2 183,929 - EX31_RS22175 0.71 +1.7 183,929 - EX31_RS22175 0.71 +0.2 183,929 - EX31_RS22175 0.71 -0.1 183,929 - EX31_RS22175 0.71 +1.0 183,929 - EX31_RS22175 0.71 +0.7 183,929 - EX31_RS22175 0.71 +0.6 183,929 - EX31_RS22175 0.71 -0.4 183,929 - EX31_RS22175 0.71 -2.8 183,952 - EX31_RS22175 0.74 -1.1 184,060 + +0.7 184,060 + +3.1 184,061 - -1.0 184,126 + +0.7 184,127 - +0.4 184,139 + +0.2 184,139 + -0.4 184,151 - -0.7 184,175 - EX31_RS22180 0.12 -1.9 184,194 + EX31_RS22180 0.14 +0.6 184,195 - EX31_RS22180 0.14 -1.2 184,195 - EX31_RS22180 0.14 +1.1 184,204 + EX31_RS22180 0.15 +0.0 184,204 + EX31_RS22180 0.15 +0.2 184,205 - EX31_RS22180 0.15 +0.9 184,205 - EX31_RS22180 0.15 +1.1 184,205 - EX31_RS22180 0.15 +0.3 184,205 - EX31_RS22180 0.15 +1.4 184,205 - EX31_RS22180 0.15 +0.9 184,205 - EX31_RS22180 0.15 -0.6 184,205 - EX31_RS22180 0.15 -1.5 184,206 + EX31_RS22180 0.15 +3.1 184,207 - EX31_RS22180 0.15 -0.5 184,207 - EX31_RS22180 0.15 +0.3 184,207 - EX31_RS22180 0.15 -0.2 184,207 - EX31_RS22180 0.15 +1.1 184,287 + EX31_RS22180 0.24 +0.7 184,383 + EX31_RS22180 0.34 +0.8 184,384 - EX31_RS22180 0.34 +0.1 184,413 + EX31_RS22180 0.38 +0.1 184,413 + EX31_RS22180 0.38 +0.3
Or see this region's nucleotide sequence