Experiment: L-Serine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS16280 and EX31_RS16285 are separated by 774 nucleotides EX31_RS16285 and EX31_RS16290 are separated by 31 nucleotides EX31_RS16290 and EX31_RS16295 are separated by 195 nucleotides
EX31_RS16280: EX31_RS16280 - lytic transglycosylase domain-containing protein, at 5,792 to 6,331
_RS16280
EX31_RS16285: EX31_RS16285 - conjugal transfer protein, at 7,106 to 7,495
_RS16285
EX31_RS16290: EX31_RS16290 - Arc family DNA-binding protein, at 7,527 to 8,153
_RS16290
EX31_RS16295: EX31_RS16295 - conjugal transfer protein, at 8,349 to 8,774
_RS16295
Position (kb)
7
8 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 6.127 kb on + strand, within EX31_RS16280 at 6.158 kb on - strand, within EX31_RS16280 at 6.158 kb on - strand, within EX31_RS16280 at 6.243 kb on + strand, within EX31_RS16280 at 6.263 kb on - strand, within EX31_RS16280 at 6.338 kb on - strand at 6.339 kb on + strand at 6.415 kb on + strand at 6.416 kb on - strand at 6.416 kb on - strand at 6.423 kb on + strand at 6.498 kb on + strand at 6.499 kb on - strand at 6.575 kb on - strand at 6.575 kb on - strand at 6.605 kb on + strand at 6.606 kb on - strand at 6.617 kb on + strand at 6.618 kb on - strand at 6.618 kb on - strand at 6.640 kb on + strand at 6.771 kb on + strand at 6.825 kb on + strand at 7.003 kb on + strand at 7.004 kb on - strand at 7.018 kb on + strand at 7.040 kb on - strand at 7.040 kb on - strand at 7.042 kb on + strand at 7.043 kb on - strand at 7.043 kb on - strand at 7.043 kb on - strand at 7.043 kb on - strand at 7.075 kb on + strand at 7.120 kb on + strand at 7.121 kb on - strand at 7.124 kb on + strand at 7.276 kb on + strand, within EX31_RS16285 at 7.276 kb on + strand, within EX31_RS16285 at 7.276 kb on + strand, within EX31_RS16285 at 7.309 kb on - strand, within EX31_RS16285 at 7.312 kb on - strand, within EX31_RS16285 at 7.349 kb on - strand, within EX31_RS16285 at 7.374 kb on - strand, within EX31_RS16285 at 7.537 kb on - strand at 7.540 kb on + strand at 7.540 kb on + strand at 7.540 kb on + strand at 7.541 kb on - strand at 7.541 kb on - strand at 7.541 kb on - strand at 7.648 kb on - strand, within EX31_RS16290 at 7.685 kb on - strand, within EX31_RS16290 at 7.728 kb on - strand, within EX31_RS16290 at 7.739 kb on + strand, within EX31_RS16290 at 7.771 kb on - strand, within EX31_RS16290 at 7.829 kb on + strand, within EX31_RS16290 at 7.892 kb on + strand, within EX31_RS16290 at 7.892 kb on + strand, within EX31_RS16290 at 7.946 kb on + strand, within EX31_RS16290 at 7.946 kb on + strand, within EX31_RS16290 at 7.947 kb on - strand, within EX31_RS16290 at 7.950 kb on - strand, within EX31_RS16290 at 7.950 kb on - strand, within EX31_RS16290 at 8.017 kb on - strand, within EX31_RS16290 at 8.053 kb on - strand, within EX31_RS16290 at 8.053 kb on - strand, within EX31_RS16290 at 8.102 kb on + strand at 8.102 kb on + strand at 8.102 kb on + strand at 8.103 kb on - strand at 8.103 kb on - strand at 8.103 kb on - strand at 8.103 kb on - strand at 8.104 kb on + strand at 8.105 kb on - strand at 8.131 kb on + strand at 8.131 kb on + strand at 8.131 kb on + strand at 8.131 kb on + strand at 8.132 kb on - strand at 8.132 kb on - strand at 8.132 kb on - strand at 8.132 kb on - strand at 8.132 kb on - strand at 8.132 kb on - strand at 8.133 kb on + strand at 8.133 kb on + strand at 8.133 kb on + strand at 8.133 kb on + strand at 8.134 kb on - strand at 8.134 kb on - strand at 8.134 kb on - strand at 8.145 kb on + strand at 8.174 kb on + strand at 8.175 kb on - strand at 8.200 kb on + strand at 8.201 kb on - strand at 8.201 kb on - strand at 8.201 kb on - strand at 8.201 kb on - strand at 8.223 kb on + strand at 8.223 kb on + strand at 8.224 kb on - strand at 8.229 kb on - strand at 8.249 kb on + strand at 8.250 kb on - strand at 8.279 kb on + strand at 8.279 kb on + strand at 8.284 kb on + strand at 8.284 kb on + strand at 8.285 kb on - strand at 8.295 kb on + strand at 8.295 kb on + strand at 8.295 kb on + strand at 8.296 kb on - strand at 8.323 kb on + strand at 8.326 kb on + strand at 8.326 kb on + strand at 8.326 kb on + strand at 8.330 kb on + strand at 8.331 kb on - strand at 8.417 kb on + strand, within EX31_RS16295 at 8.417 kb on + strand, within EX31_RS16295 at 8.417 kb on + strand, within EX31_RS16295 at 8.417 kb on + strand, within EX31_RS16295 at 8.417 kb on + strand, within EX31_RS16295 at 8.417 kb on + strand, within EX31_RS16295 at 8.417 kb on + strand, within EX31_RS16295 at 8.418 kb on - strand, within EX31_RS16295 at 8.418 kb on - strand, within EX31_RS16295 at 8.418 kb on - strand, within EX31_RS16295 at 8.418 kb on - strand, within EX31_RS16295 at 8.418 kb on - strand, within EX31_RS16295 at 8.418 kb on - strand, within EX31_RS16295 at 8.455 kb on - strand, within EX31_RS16295 at 8.460 kb on - strand, within EX31_RS16295 at 8.480 kb on + strand, within EX31_RS16295 at 8.484 kb on - strand, within EX31_RS16295
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); ShewMM media; no shaking remove 6,127 + EX31_RS16280 0.62 +0.7 6,158 - EX31_RS16280 0.68 +0.3 6,158 - EX31_RS16280 0.68 -0.5 6,243 + EX31_RS16280 0.84 -0.3 6,263 - EX31_RS16280 0.87 +0.3 6,338 - +0.3 6,339 + -0.6 6,415 + +1.9 6,416 - +0.0 6,416 - -0.4 6,423 + -0.1 6,498 + +0.5 6,499 - -0.1 6,575 - +0.7 6,575 - -0.3 6,605 + -0.1 6,606 - +0.7 6,617 + +0.6 6,618 - +1.0 6,618 - +0.7 6,640 + -0.7 6,771 + -0.2 6,825 + +0.2 7,003 + -0.7 7,004 - +2.7 7,018 + +1.5 7,040 - +0.3 7,040 - -0.2 7,042 + +0.9 7,043 - +2.0 7,043 - +0.5 7,043 - -1.1 7,043 - +1.1 7,075 + +0.6 7,120 + -0.7 7,121 - +0.9 7,124 + -0.6 7,276 + EX31_RS16285 0.44 -1.6 7,276 + EX31_RS16285 0.44 +0.6 7,276 + EX31_RS16285 0.44 -2.5 7,309 - EX31_RS16285 0.52 +1.3 7,312 - EX31_RS16285 0.53 +0.7 7,349 - EX31_RS16285 0.62 +0.2 7,374 - EX31_RS16285 0.69 +0.3 7,537 - +0.6 7,540 + +0.9 7,540 + -0.5 7,540 + -0.2 7,541 - +0.5 7,541 - -0.2 7,541 - +0.3 7,648 - EX31_RS16290 0.19 -0.5 7,685 - EX31_RS16290 0.25 -1.2 7,728 - EX31_RS16290 0.32 -0.7 7,739 + EX31_RS16290 0.34 +0.7 7,771 - EX31_RS16290 0.39 +0.3 7,829 + EX31_RS16290 0.48 +1.6 7,892 + EX31_RS16290 0.58 +0.3 7,892 + EX31_RS16290 0.58 +1.8 7,946 + EX31_RS16290 0.67 +1.3 7,946 + EX31_RS16290 0.67 -1.7 7,947 - EX31_RS16290 0.67 -1.0 7,950 - EX31_RS16290 0.67 -0.5 7,950 - EX31_RS16290 0.67 -0.0 8,017 - EX31_RS16290 0.78 -0.7 8,053 - EX31_RS16290 0.84 +0.3 8,053 - EX31_RS16290 0.84 +1.0 8,102 + -0.9 8,102 + +0.2 8,102 + +0.5 8,103 - -0.9 8,103 - +0.7 8,103 - -0.1 8,103 - +0.6 8,104 + +0.9 8,105 - +1.1 8,131 + -0.8 8,131 + +0.2 8,131 + +0.1 8,131 + +0.4 8,132 - +0.1 8,132 - -1.1 8,132 - -0.7 8,132 - -1.2 8,132 - -1.3 8,132 - -0.1 8,133 + -0.3 8,133 + -0.7 8,133 + +0.5 8,133 + +1.3 8,134 - -0.7 8,134 - -0.7 8,134 - -0.2 8,145 + +0.5 8,174 + +0.3 8,175 - +1.4 8,200 + +0.8 8,201 - +0.5 8,201 - -0.4 8,201 - +0.6 8,201 - +0.8 8,223 + -1.3 8,223 + +0.5 8,224 - -0.7 8,229 - +0.2 8,249 + +0.2 8,250 - +0.4 8,279 + +1.0 8,279 + +0.7 8,284 + +0.9 8,284 + +1.3 8,285 - -0.8 8,295 + -2.1 8,295 + +0.6 8,295 + +0.8 8,296 - +1.1 8,323 + -0.6 8,326 + +1.9 8,326 + -1.5 8,326 + -0.3 8,330 + +0.4 8,331 - +1.4 8,417 + EX31_RS16295 0.16 -0.3 8,417 + EX31_RS16295 0.16 -0.7 8,417 + EX31_RS16295 0.16 -0.1 8,417 + EX31_RS16295 0.16 +3.3 8,417 + EX31_RS16295 0.16 -0.1 8,417 + EX31_RS16295 0.16 +1.1 8,417 + EX31_RS16295 0.16 +0.8 8,418 - EX31_RS16295 0.16 +1.4 8,418 - EX31_RS16295 0.16 +2.3 8,418 - EX31_RS16295 0.16 +0.2 8,418 - EX31_RS16295 0.16 -0.1 8,418 - EX31_RS16295 0.16 +0.3 8,418 - EX31_RS16295 0.16 +1.7 8,455 - EX31_RS16295 0.25 +0.7 8,460 - EX31_RS16295 0.26 +0.6 8,480 + EX31_RS16295 0.31 +0.6 8,484 - EX31_RS16295 0.32 +0.7
Or see this region's nucleotide sequence