Experiment: L-Serine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS10975 and EX31_RS10980 are separated by 43 nucleotides EX31_RS10980 and EX31_RS10985 are separated by 357 nucleotides
EX31_RS10975: EX31_RS10975 - DUF2891 domain-containing protein, at 122,786 to 123,808
_RS10975
EX31_RS10980: EX31_RS10980 - acyl-ACP--UDP-N-acetylglucosamine O-acyltransferase, at 123,852 to 124,643
_RS10980
EX31_RS10985: EX31_RS10985 - phosphoethanolamine--lipid A transferase, at 125,001 to 126,635
_RS10985
Position (kb) 123
124
125 Strain fitness (log2 ratio) -2
-1
0
1
2 at 122.931 kb on + strand, within EX31_RS10975 at 122.931 kb on + strand, within EX31_RS10975 at 122.932 kb on - strand, within EX31_RS10975 at 122.932 kb on - strand, within EX31_RS10975 at 122.932 kb on - strand, within EX31_RS10975 at 122.954 kb on + strand, within EX31_RS10975 at 122.954 kb on + strand, within EX31_RS10975 at 122.955 kb on - strand, within EX31_RS10975 at 122.955 kb on - strand, within EX31_RS10975 at 122.988 kb on + strand, within EX31_RS10975 at 123.022 kb on - strand, within EX31_RS10975 at 123.335 kb on + strand, within EX31_RS10975 at 123.755 kb on + strand at 123.755 kb on + strand at 123.755 kb on + strand at 123.755 kb on + strand at 123.755 kb on + strand at 123.755 kb on + strand at 123.755 kb on + strand at 123.755 kb on + strand at 123.755 kb on + strand at 123.755 kb on + strand at 123.755 kb on + strand at 123.756 kb on - strand at 123.756 kb on - strand at 123.756 kb on - strand at 123.756 kb on - strand at 123.756 kb on - strand at 123.986 kb on - strand, within EX31_RS10980 at 123.992 kb on - strand, within EX31_RS10980 at 124.118 kb on + strand, within EX31_RS10980 at 124.118 kb on + strand, within EX31_RS10980 at 124.118 kb on + strand, within EX31_RS10980 at 124.119 kb on - strand, within EX31_RS10980 at 124.119 kb on - strand, within EX31_RS10980 at 124.122 kb on - strand, within EX31_RS10980 at 124.122 kb on - strand, within EX31_RS10980 at 124.149 kb on - strand, within EX31_RS10980 at 124.151 kb on + strand, within EX31_RS10980 at 124.151 kb on + strand, within EX31_RS10980 at 124.152 kb on - strand, within EX31_RS10980 at 124.255 kb on - strand, within EX31_RS10980 at 124.412 kb on - strand, within EX31_RS10980 at 124.599 kb on + strand at 124.599 kb on + strand at 124.600 kb on - strand at 124.604 kb on + strand at 124.604 kb on + strand at 124.605 kb on - strand at 124.685 kb on - strand at 124.699 kb on + strand at 124.700 kb on - strand at 124.800 kb on + strand at 124.800 kb on + strand at 124.893 kb on - strand at 124.923 kb on - strand at 124.944 kb on + strand at 125.009 kb on + strand at 125.010 kb on - strand at 125.012 kb on - strand at 125.029 kb on - strand at 125.044 kb on + strand at 125.059 kb on - strand at 125.110 kb on + strand at 125.111 kb on - strand at 125.228 kb on + strand, within EX31_RS10985 at 125.229 kb on - strand, within EX31_RS10985 at 125.287 kb on + strand, within EX31_RS10985 at 125.288 kb on - strand, within EX31_RS10985 at 125.312 kb on + strand, within EX31_RS10985 at 125.346 kb on + strand, within EX31_RS10985 at 125.347 kb on - strand, within EX31_RS10985 at 125.365 kb on + strand, within EX31_RS10985 at 125.400 kb on - strand, within EX31_RS10985 at 125.400 kb on - strand, within EX31_RS10985 at 125.526 kb on + strand, within EX31_RS10985 at 125.583 kb on + strand, within EX31_RS10985 at 125.584 kb on - strand, within EX31_RS10985 at 125.604 kb on + strand, within EX31_RS10985 at 125.619 kb on + strand, within EX31_RS10985 at 125.620 kb on - strand, within EX31_RS10985
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); ShewMM media; no shaking remove 122,931 + EX31_RS10975 0.14 +0.8 122,931 + EX31_RS10975 0.14 -0.0 122,932 - EX31_RS10975 0.14 +0.1 122,932 - EX31_RS10975 0.14 +1.0 122,932 - EX31_RS10975 0.14 -1.9 122,954 + EX31_RS10975 0.16 -0.3 122,954 + EX31_RS10975 0.16 -0.6 122,955 - EX31_RS10975 0.17 +1.5 122,955 - EX31_RS10975 0.17 +0.9 122,988 + EX31_RS10975 0.20 +0.3 123,022 - EX31_RS10975 0.23 -0.0 123,335 + EX31_RS10975 0.54 +0.4 123,755 + +0.2 123,755 + +1.2 123,755 + +0.5 123,755 + +0.5 123,755 + -0.5 123,755 + +1.5 123,755 + -0.0 123,755 + +1.3 123,755 + +0.5 123,755 + +1.5 123,755 + -0.8 123,756 - +1.1 123,756 - +0.3 123,756 - +1.1 123,756 - +0.8 123,756 - +1.0 123,986 - EX31_RS10980 0.17 +0.9 123,992 - EX31_RS10980 0.18 +0.8 124,118 + EX31_RS10980 0.34 +0.7 124,118 + EX31_RS10980 0.34 +2.0 124,118 + EX31_RS10980 0.34 +1.8 124,119 - EX31_RS10980 0.34 +1.9 124,119 - EX31_RS10980 0.34 +1.0 124,122 - EX31_RS10980 0.34 -0.2 124,122 - EX31_RS10980 0.34 +2.1 124,149 - EX31_RS10980 0.38 +0.8 124,151 + EX31_RS10980 0.38 +0.3 124,151 + EX31_RS10980 0.38 -0.6 124,152 - EX31_RS10980 0.38 +1.7 124,255 - EX31_RS10980 0.51 +0.4 124,412 - EX31_RS10980 0.71 -0.8 124,599 + +0.9 124,599 + +0.3 124,600 - -0.5 124,604 + -0.5 124,604 + -0.5 124,605 - -0.4 124,685 - -0.6 124,699 + +0.4 124,700 - +0.9 124,800 + -0.0 124,800 + -0.0 124,893 - +0.2 124,923 - -0.2 124,944 + +0.8 125,009 + -0.0 125,010 - +2.1 125,012 - -0.1 125,029 - -0.5 125,044 + +0.4 125,059 - +0.7 125,110 + +0.5 125,111 - +0.3 125,228 + EX31_RS10985 0.14 +1.0 125,229 - EX31_RS10985 0.14 -1.5 125,287 + EX31_RS10985 0.17 +0.3 125,288 - EX31_RS10985 0.18 +1.4 125,312 + EX31_RS10985 0.19 +0.2 125,346 + EX31_RS10985 0.21 +1.1 125,347 - EX31_RS10985 0.21 +2.1 125,365 + EX31_RS10985 0.22 +0.2 125,400 - EX31_RS10985 0.24 -0.8 125,400 - EX31_RS10985 0.24 -0.5 125,526 + EX31_RS10985 0.32 -0.7 125,583 + EX31_RS10985 0.36 -0.0 125,584 - EX31_RS10985 0.36 -0.8 125,604 + EX31_RS10985 0.37 -0.3 125,619 + EX31_RS10985 0.38 +1.0 125,620 - EX31_RS10985 0.38 -0.3
Or see this region's nucleotide sequence