Strain Fitness in Rahnella sp. WP5 around EX31_RS10215

Experiment: L-Serine (N); ShewMM media; no shaking

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntEX31_RS10210 and EX31_RS10215 overlap by 8 nucleotidesEX31_RS10215 and EX31_RS10220 are separated by 324 nucleotides EX31_RS10210: EX31_RS10210 - 4-oxalomesaconate tautomerase, at 254,858 to 255,982 _RS10210 EX31_RS10215: EX31_RS10215 - NAD(P)-dependent oxidoreductase, at 255,975 to 256,859 _RS10215 EX31_RS10220: EX31_RS10220 - ABC transporter ATP-binding protein, at 257,184 to 258,140 _RS10220 Position (kb) 255 256 257Strain fitness (log2 ratio) -2 -1 0 1at 255.103 kb on + strand, within EX31_RS10210at 255.104 kb on - strand, within EX31_RS10210at 255.148 kb on + strand, within EX31_RS10210at 255.211 kb on + strand, within EX31_RS10210at 255.290 kb on + strand, within EX31_RS10210at 255.290 kb on + strand, within EX31_RS10210at 255.668 kb on - strand, within EX31_RS10210at 255.668 kb on - strand, within EX31_RS10210at 255.700 kb on - strand, within EX31_RS10210at 255.767 kb on - strand, within EX31_RS10210at 255.767 kb on - strand, within EX31_RS10210at 255.831 kb on + strand, within EX31_RS10210at 255.831 kb on + strand, within EX31_RS10210at 255.886 kb on - strandat 255.886 kb on - strandat 255.890 kb on - strandat 255.981 kb on - strandat 256.082 kb on + strand, within EX31_RS10215at 256.082 kb on + strand, within EX31_RS10215at 256.083 kb on - strand, within EX31_RS10215at 256.083 kb on - strand, within EX31_RS10215at 256.332 kb on + strand, within EX31_RS10215at 256.333 kb on - strand, within EX31_RS10215at 256.410 kb on + strand, within EX31_RS10215at 256.753 kb on + strand, within EX31_RS10215at 256.753 kb on + strand, within EX31_RS10215at 256.754 kb on - strand, within EX31_RS10215at 256.754 kb on - strand, within EX31_RS10215at 256.804 kb on - strandat 256.804 kb on - strandat 256.805 kb on + strandat 256.806 kb on - strandat 256.840 kb on + strandat 256.857 kb on + strandat 256.858 kb on - strandat 256.858 kb on - strandat 257.117 kb on + strandat 257.179 kb on - strandat 257.179 kb on - strandat 257.186 kb on - strandat 257.299 kb on + strand, within EX31_RS10220at 257.300 kb on - strand, within EX31_RS10220at 257.300 kb on - strand, within EX31_RS10220at 257.331 kb on + strand, within EX31_RS10220at 257.332 kb on - strand, within EX31_RS10220at 257.410 kb on + strand, within EX31_RS10220at 257.410 kb on + strand, within EX31_RS10220at 257.411 kb on - strand, within EX31_RS10220at 257.434 kb on - strand, within EX31_RS10220at 257.473 kb on + strand, within EX31_RS10220at 257.473 kb on + strand, within EX31_RS10220at 257.473 kb on + strand, within EX31_RS10220at 257.473 kb on + strand, within EX31_RS10220at 257.536 kb on + strand, within EX31_RS10220at 257.537 kb on - strand, within EX31_RS10220at 257.537 kb on - strand, within EX31_RS10220at 257.537 kb on - strand, within EX31_RS10220at 257.537 kb on - strand, within EX31_RS10220at 257.537 kb on - strand, within EX31_RS10220at 257.546 kb on + strand, within EX31_RS10220at 257.666 kb on + strand, within EX31_RS10220at 257.672 kb on + strand, within EX31_RS10220at 257.672 kb on + strand, within EX31_RS10220at 257.672 kb on + strand, within EX31_RS10220at 257.673 kb on - strand, within EX31_RS10220

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Serine (N); ShewMM media; no shaking
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255,103 + EX31_RS10210 0.22 +0.3
255,104 - EX31_RS10210 0.22 +1.0
255,148 + EX31_RS10210 0.26 -0.4
255,211 + EX31_RS10210 0.31 +0.1
255,290 + EX31_RS10210 0.38 +1.0
255,290 + EX31_RS10210 0.38 +0.3
255,668 - EX31_RS10210 0.72 +1.4
255,668 - EX31_RS10210 0.72 -2.1
255,700 - EX31_RS10210 0.75 +0.2
255,767 - EX31_RS10210 0.81 +0.6
255,767 - EX31_RS10210 0.81 -0.1
255,831 + EX31_RS10210 0.86 +1.1
255,831 + EX31_RS10210 0.86 -1.6
255,886 - -0.7
255,886 - +0.6
255,890 - -0.1
255,981 - +0.1
256,082 + EX31_RS10215 0.12 -1.2
256,082 + EX31_RS10215 0.12 -1.0
256,083 - EX31_RS10215 0.12 -0.6
256,083 - EX31_RS10215 0.12 -1.2
256,332 + EX31_RS10215 0.40 +0.4
256,333 - EX31_RS10215 0.40 -0.6
256,410 + EX31_RS10215 0.49 -1.2
256,753 + EX31_RS10215 0.88 -2.3
256,753 + EX31_RS10215 0.88 +0.7
256,754 - EX31_RS10215 0.88 -0.9
256,754 - EX31_RS10215 0.88 -1.2
256,804 - -1.1
256,804 - +0.3
256,805 + -0.6
256,806 - +1.6
256,840 + -1.9
256,857 + -0.8
256,858 - -0.6
256,858 - -0.4
257,117 + +0.8
257,179 - -0.1
257,179 - -0.2
257,186 - -0.8
257,299 + EX31_RS10220 0.12 +0.4
257,300 - EX31_RS10220 0.12 +0.4
257,300 - EX31_RS10220 0.12 +0.4
257,331 + EX31_RS10220 0.15 +0.7
257,332 - EX31_RS10220 0.15 +0.0
257,410 + EX31_RS10220 0.24 -0.5
257,410 + EX31_RS10220 0.24 +1.4
257,411 - EX31_RS10220 0.24 -1.1
257,434 - EX31_RS10220 0.26 +0.5
257,473 + EX31_RS10220 0.30 +1.4
257,473 + EX31_RS10220 0.30 -0.1
257,473 + EX31_RS10220 0.30 -1.4
257,473 + EX31_RS10220 0.30 -0.1
257,536 + EX31_RS10220 0.37 +0.4
257,537 - EX31_RS10220 0.37 +0.4
257,537 - EX31_RS10220 0.37 +0.7
257,537 - EX31_RS10220 0.37 +0.1
257,537 - EX31_RS10220 0.37 -0.2
257,537 - EX31_RS10220 0.37 +0.9
257,546 + EX31_RS10220 0.38 +0.7
257,666 + EX31_RS10220 0.50 +1.0
257,672 + EX31_RS10220 0.51 -0.8
257,672 + EX31_RS10220 0.51 -0.6
257,672 + EX31_RS10220 0.51 -1.3
257,673 - EX31_RS10220 0.51 -0.5

Or see this region's nucleotide sequence