Experiment: L-Serine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS10000 and EX31_RS10005 are separated by 99 nucleotides EX31_RS10005 and EX31_RS10010 are separated by 73 nucleotides EX31_RS10010 and EX31_RS10015 are separated by 101 nucleotides
EX31_RS10000: EX31_RS10000 - UPF0259 family protein, at 208,577 to 209,332
_RS10000
EX31_RS10005: EX31_RS10005 - septation protein A, at 209,432 to 209,971
_RS10005
EX31_RS10010: EX31_RS10010 - acyl-CoA thioester hydrolase YciA, at 210,045 to 210,485
_RS10010
EX31_RS10015: EX31_RS10015 - energy transducer TonB, at 210,587 to 211,318
_RS10015
Position (kb) 210
211 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 209.287 kb on + strand at 209.297 kb on + strand at 209.297 kb on + strand at 209.312 kb on + strand at 209.322 kb on + strand at 209.330 kb on + strand at 209.330 kb on + strand at 209.330 kb on + strand at 209.330 kb on + strand at 209.330 kb on + strand at 209.330 kb on + strand at 209.330 kb on + strand at 209.333 kb on + strand at 209.334 kb on - strand at 209.335 kb on + strand at 209.335 kb on + strand at 209.335 kb on + strand at 209.341 kb on + strand at 209.341 kb on + strand at 209.341 kb on + strand at 209.341 kb on + strand at 209.341 kb on + strand at 209.397 kb on + strand at 209.399 kb on + strand at 209.410 kb on + strand at 209.410 kb on + strand at 209.410 kb on + strand at 209.414 kb on + strand at 209.414 kb on + strand at 209.740 kb on + strand, within EX31_RS10005 at 209.933 kb on + strand at 209.935 kb on + strand at 209.969 kb on + strand at 210.044 kb on + strand at 210.059 kb on + strand at 210.059 kb on + strand at 210.059 kb on + strand at 210.060 kb on - strand at 210.296 kb on + strand, within EX31_RS10010 at 210.299 kb on + strand, within EX31_RS10010 at 210.299 kb on + strand, within EX31_RS10010 at 210.299 kb on + strand, within EX31_RS10010 at 210.299 kb on + strand, within EX31_RS10010 at 210.300 kb on - strand, within EX31_RS10010 at 210.366 kb on + strand, within EX31_RS10010 at 210.366 kb on + strand, within EX31_RS10010 at 210.389 kb on + strand, within EX31_RS10010 at 210.390 kb on - strand, within EX31_RS10010 at 210.393 kb on + strand, within EX31_RS10010 at 210.393 kb on + strand, within EX31_RS10010 at 210.393 kb on + strand, within EX31_RS10010 at 210.394 kb on - strand, within EX31_RS10010 at 210.403 kb on + strand, within EX31_RS10010 at 210.496 kb on + strand at 210.497 kb on - strand at 210.497 kb on - strand at 210.508 kb on + strand at 210.508 kb on + strand at 210.588 kb on + strand at 211.167 kb on - strand, within EX31_RS10015 at 211.225 kb on - strand, within EX31_RS10015 at 211.319 kb on - strand at 211.362 kb on - strand at 211.392 kb on + strand at 211.393 kb on - strand at 211.393 kb on - strand at 211.451 kb on + strand at 211.452 kb on - strand at 211.452 kb on - strand at 211.455 kb on + strand at 211.455 kb on + strand at 211.455 kb on + strand at 211.455 kb on + strand at 211.455 kb on + strand at 211.456 kb on - strand at 211.456 kb on - strand at 211.456 kb on - strand at 211.460 kb on + strand at 211.460 kb on + strand at 211.460 kb on + strand at 211.461 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); ShewMM media; no shaking remove 209,287 + -1.4 209,297 + -0.2 209,297 + -1.2 209,312 + -1.2 209,322 + -0.2 209,330 + +2.2 209,330 + -0.0 209,330 + +0.6 209,330 + +1.2 209,330 + -0.5 209,330 + +1.1 209,330 + +0.7 209,333 + +1.1 209,334 - -0.0 209,335 + +0.7 209,335 + +0.6 209,335 + -1.8 209,341 + -0.1 209,341 + +0.3 209,341 + -0.1 209,341 + +0.3 209,341 + +0.3 209,397 + -0.8 209,399 + -0.3 209,410 + +1.0 209,410 + +1.1 209,410 + +0.6 209,414 + -0.7 209,414 + +0.7 209,740 + EX31_RS10005 0.57 -0.3 209,933 + +2.2 209,935 + +0.7 209,969 + -0.1 210,044 + +1.4 210,059 + +0.2 210,059 + -0.2 210,059 + -0.6 210,060 - -1.4 210,296 + EX31_RS10010 0.57 +0.6 210,299 + EX31_RS10010 0.58 +0.7 210,299 + EX31_RS10010 0.58 -0.2 210,299 + EX31_RS10010 0.58 +0.9 210,299 + EX31_RS10010 0.58 +1.4 210,300 - EX31_RS10010 0.58 +1.4 210,366 + EX31_RS10010 0.73 +0.3 210,366 + EX31_RS10010 0.73 -0.7 210,389 + EX31_RS10010 0.78 +0.8 210,390 - EX31_RS10010 0.78 +0.4 210,393 + EX31_RS10010 0.79 +0.5 210,393 + EX31_RS10010 0.79 -0.9 210,393 + EX31_RS10010 0.79 +1.0 210,394 - EX31_RS10010 0.79 +0.6 210,403 + EX31_RS10010 0.81 +0.2 210,496 + +0.6 210,497 - +0.7 210,497 - -0.1 210,508 + -0.2 210,508 + +0.4 210,588 + -0.9 211,167 - EX31_RS10015 0.79 -1.4 211,225 - EX31_RS10015 0.87 -1.8 211,319 - -0.1 211,362 - -0.8 211,392 + -0.6 211,393 - -0.3 211,393 - -0.5 211,451 + -0.4 211,452 - +0.5 211,452 - +1.2 211,455 + +1.7 211,455 + -0.2 211,455 + -1.1 211,455 + -0.3 211,455 + -0.4 211,456 - -0.1 211,456 - -2.9 211,456 - +0.9 211,460 + -0.5 211,460 + +0.4 211,460 + -1.8 211,461 - +1.1
Or see this region's nucleotide sequence