Experiment: L-Serine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS09525 and EX31_RS09530 are separated by 3 nucleotides EX31_RS09530 and EX31_RS09535 are separated by 855 nucleotides
EX31_RS09525: EX31_RS09525 - bestrophin, at 109,474 to 110,379
_RS09525
EX31_RS09530: EX31_RS09530 - hypothetical protein, at 110,383 to 110,628
_RS09530
EX31_RS09535: EX31_RS09535 - flagellar hook-basal body complex protein FliE, at 111,484 to 111,798
_RS09535
Position (kb) 110
111 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 109.397 kb on + strand at 109.475 kb on + strand at 109.475 kb on + strand at 109.476 kb on - strand at 109.476 kb on - strand at 109.476 kb on - strand at 109.476 kb on - strand at 109.555 kb on + strand at 109.569 kb on + strand, within EX31_RS09525 at 109.569 kb on + strand, within EX31_RS09525 at 109.569 kb on + strand, within EX31_RS09525 at 109.570 kb on - strand, within EX31_RS09525 at 109.570 kb on - strand, within EX31_RS09525 at 109.570 kb on - strand, within EX31_RS09525 at 109.570 kb on - strand, within EX31_RS09525 at 109.570 kb on - strand, within EX31_RS09525 at 109.591 kb on - strand, within EX31_RS09525 at 109.633 kb on - strand, within EX31_RS09525 at 109.652 kb on + strand, within EX31_RS09525 at 109.653 kb on - strand, within EX31_RS09525 at 109.723 kb on - strand, within EX31_RS09525 at 109.723 kb on - strand, within EX31_RS09525 at 109.733 kb on + strand, within EX31_RS09525 at 109.733 kb on + strand, within EX31_RS09525 at 109.733 kb on + strand, within EX31_RS09525 at 109.734 kb on - strand, within EX31_RS09525 at 109.734 kb on - strand, within EX31_RS09525 at 109.735 kb on + strand, within EX31_RS09525 at 109.736 kb on - strand, within EX31_RS09525 at 109.761 kb on - strand, within EX31_RS09525 at 109.782 kb on + strand, within EX31_RS09525 at 109.823 kb on - strand, within EX31_RS09525 at 109.823 kb on - strand, within EX31_RS09525 at 109.845 kb on + strand, within EX31_RS09525 at 109.845 kb on + strand, within EX31_RS09525 at 109.847 kb on + strand, within EX31_RS09525 at 109.918 kb on - strand, within EX31_RS09525 at 109.918 kb on - strand, within EX31_RS09525 at 109.918 kb on - strand, within EX31_RS09525 at 109.918 kb on - strand, within EX31_RS09525 at 110.092 kb on + strand, within EX31_RS09525 at 110.093 kb on - strand, within EX31_RS09525 at 110.093 kb on - strand, within EX31_RS09525 at 110.093 kb on - strand, within EX31_RS09525 at 110.126 kb on - strand, within EX31_RS09525 at 110.143 kb on - strand, within EX31_RS09525 at 110.144 kb on + strand, within EX31_RS09525 at 110.145 kb on - strand, within EX31_RS09525 at 110.145 kb on - strand, within EX31_RS09525 at 110.191 kb on - strand, within EX31_RS09525 at 110.247 kb on - strand, within EX31_RS09525 at 110.396 kb on - strand at 110.433 kb on + strand, within EX31_RS09530 at 110.433 kb on + strand, within EX31_RS09530 at 110.433 kb on + strand, within EX31_RS09530 at 110.433 kb on + strand, within EX31_RS09530 at 110.434 kb on - strand, within EX31_RS09530 at 110.434 kb on - strand, within EX31_RS09530 at 110.540 kb on - strand, within EX31_RS09530 at 110.560 kb on + strand, within EX31_RS09530 at 110.560 kb on + strand, within EX31_RS09530 at 110.560 kb on + strand, within EX31_RS09530 at 110.560 kb on + strand, within EX31_RS09530 at 110.561 kb on - strand, within EX31_RS09530 at 110.561 kb on - strand, within EX31_RS09530 at 110.571 kb on + strand, within EX31_RS09530 at 110.584 kb on - strand, within EX31_RS09530 at 110.712 kb on - strand at 110.717 kb on + strand at 110.722 kb on - strand at 110.771 kb on + strand at 110.771 kb on + strand at 110.772 kb on - strand at 110.923 kb on - strand at 110.956 kb on + strand at 111.023 kb on + strand at 111.161 kb on + strand at 111.183 kb on - strand at 111.467 kb on - strand at 111.486 kb on - strand at 111.529 kb on - strand, within EX31_RS09535 at 111.529 kb on - strand, within EX31_RS09535 at 111.529 kb on - strand, within EX31_RS09535 at 111.535 kb on + strand, within EX31_RS09535
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); ShewMM media; no shaking remove 109,397 + +2.4 109,475 + +0.1 109,475 + -0.2 109,476 - -1.4 109,476 - -0.6 109,476 - +0.4 109,476 - +1.1 109,555 + +0.1 109,569 + EX31_RS09525 0.10 +4.4 109,569 + EX31_RS09525 0.10 -0.8 109,569 + EX31_RS09525 0.10 +1.0 109,570 - EX31_RS09525 0.11 -0.5 109,570 - EX31_RS09525 0.11 -2.1 109,570 - EX31_RS09525 0.11 +1.4 109,570 - EX31_RS09525 0.11 -0.3 109,570 - EX31_RS09525 0.11 +0.1 109,591 - EX31_RS09525 0.13 -0.7 109,633 - EX31_RS09525 0.18 -0.0 109,652 + EX31_RS09525 0.20 -0.2 109,653 - EX31_RS09525 0.20 +0.4 109,723 - EX31_RS09525 0.27 +1.0 109,723 - EX31_RS09525 0.27 +1.4 109,733 + EX31_RS09525 0.29 +1.5 109,733 + EX31_RS09525 0.29 +1.5 109,733 + EX31_RS09525 0.29 -1.1 109,734 - EX31_RS09525 0.29 -0.2 109,734 - EX31_RS09525 0.29 +0.8 109,735 + EX31_RS09525 0.29 +1.4 109,736 - EX31_RS09525 0.29 -2.3 109,761 - EX31_RS09525 0.32 +0.8 109,782 + EX31_RS09525 0.34 +0.2 109,823 - EX31_RS09525 0.39 +0.0 109,823 - EX31_RS09525 0.39 +1.4 109,845 + EX31_RS09525 0.41 -0.9 109,845 + EX31_RS09525 0.41 -1.9 109,847 + EX31_RS09525 0.41 +2.4 109,918 - EX31_RS09525 0.49 +0.8 109,918 - EX31_RS09525 0.49 +0.5 109,918 - EX31_RS09525 0.49 -0.6 109,918 - EX31_RS09525 0.49 -1.8 110,092 + EX31_RS09525 0.68 +0.4 110,093 - EX31_RS09525 0.68 +0.1 110,093 - EX31_RS09525 0.68 +0.4 110,093 - EX31_RS09525 0.68 -0.6 110,126 - EX31_RS09525 0.72 +0.1 110,143 - EX31_RS09525 0.74 -0.2 110,144 + EX31_RS09525 0.74 -0.4 110,145 - EX31_RS09525 0.74 +2.1 110,145 - EX31_RS09525 0.74 +0.2 110,191 - EX31_RS09525 0.79 +0.4 110,247 - EX31_RS09525 0.85 +0.7 110,396 - -1.4 110,433 + EX31_RS09530 0.20 -2.0 110,433 + EX31_RS09530 0.20 -0.2 110,433 + EX31_RS09530 0.20 -0.9 110,433 + EX31_RS09530 0.20 -0.8 110,434 - EX31_RS09530 0.21 +0.4 110,434 - EX31_RS09530 0.21 -0.5 110,540 - EX31_RS09530 0.64 -0.2 110,560 + EX31_RS09530 0.72 -0.3 110,560 + EX31_RS09530 0.72 +1.4 110,560 + EX31_RS09530 0.72 -0.2 110,560 + EX31_RS09530 0.72 -0.9 110,561 - EX31_RS09530 0.72 -0.6 110,561 - EX31_RS09530 0.72 -0.8 110,571 + EX31_RS09530 0.76 -1.3 110,584 - EX31_RS09530 0.82 +0.2 110,712 - -1.8 110,717 + -0.1 110,722 - +0.1 110,771 + -0.9 110,771 + +0.2 110,772 - -0.1 110,923 - -0.9 110,956 + +0.8 111,023 + +0.4 111,161 + -1.0 111,183 - +0.8 111,467 - -0.4 111,486 - +0.1 111,529 - EX31_RS09535 0.14 +0.3 111,529 - EX31_RS09535 0.14 -1.0 111,529 - EX31_RS09535 0.14 -0.2 111,535 + EX31_RS09535 0.16 +1.0
Or see this region's nucleotide sequence