Experiment: L-Serine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS06350 and EX31_RS06355 are separated by 64 nucleotides EX31_RS06355 and EX31_RS06360 are separated by 113 nucleotides
EX31_RS06350: EX31_RS06350 - peptide ABC transporter substrate-binding protein, at 32,790 to 34,424
_RS06350
EX31_RS06355: EX31_RS06355 - MFS transporter, at 34,489 to 35,691
_RS06355
EX31_RS06360: EX31_RS06360 - LysR family transcriptional regulator, at 35,805 to 36,740
_RS06360
Position (kb) 34
35
36 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 33.501 kb on + strand, within EX31_RS06350 at 33.627 kb on + strand, within EX31_RS06350 at 33.663 kb on + strand, within EX31_RS06350 at 33.687 kb on + strand, within EX31_RS06350 at 33.687 kb on + strand, within EX31_RS06350 at 33.688 kb on - strand, within EX31_RS06350 at 33.688 kb on - strand, within EX31_RS06350 at 33.688 kb on - strand, within EX31_RS06350 at 33.688 kb on - strand, within EX31_RS06350 at 33.688 kb on - strand, within EX31_RS06350 at 33.690 kb on + strand, within EX31_RS06350 at 33.693 kb on + strand, within EX31_RS06350 at 33.693 kb on + strand, within EX31_RS06350 at 33.693 kb on + strand, within EX31_RS06350 at 33.758 kb on + strand, within EX31_RS06350 at 33.758 kb on + strand, within EX31_RS06350 at 33.822 kb on - strand, within EX31_RS06350 at 33.963 kb on + strand, within EX31_RS06350 at 33.963 kb on + strand, within EX31_RS06350 at 33.963 kb on + strand, within EX31_RS06350 at 33.963 kb on + strand, within EX31_RS06350 at 33.964 kb on - strand, within EX31_RS06350 at 33.964 kb on - strand, within EX31_RS06350 at 33.964 kb on - strand, within EX31_RS06350 at 33.964 kb on - strand, within EX31_RS06350 at 33.965 kb on + strand, within EX31_RS06350 at 33.966 kb on - strand, within EX31_RS06350 at 34.068 kb on + strand, within EX31_RS06350 at 34.128 kb on + strand, within EX31_RS06350 at 34.129 kb on - strand, within EX31_RS06350 at 34.211 kb on + strand, within EX31_RS06350 at 34.267 kb on - strand at 34.286 kb on + strand at 34.287 kb on - strand at 34.318 kb on - strand at 34.324 kb on - strand at 34.363 kb on - strand at 34.393 kb on - strand at 34.407 kb on + strand at 34.407 kb on + strand at 34.407 kb on + strand at 34.407 kb on + strand at 34.426 kb on - strand at 34.478 kb on + strand at 34.513 kb on + strand at 34.679 kb on + strand, within EX31_RS06355 at 34.680 kb on - strand, within EX31_RS06355 at 34.716 kb on + strand, within EX31_RS06355 at 34.717 kb on - strand, within EX31_RS06355 at 34.717 kb on - strand, within EX31_RS06355 at 34.720 kb on + strand, within EX31_RS06355 at 34.720 kb on + strand, within EX31_RS06355 at 34.721 kb on - strand, within EX31_RS06355 at 34.721 kb on - strand, within EX31_RS06355 at 34.721 kb on - strand, within EX31_RS06355 at 34.721 kb on - strand, within EX31_RS06355 at 34.888 kb on + strand, within EX31_RS06355 at 34.962 kb on + strand, within EX31_RS06355 at 34.963 kb on - strand, within EX31_RS06355 at 35.040 kb on + strand, within EX31_RS06355 at 35.171 kb on + strand, within EX31_RS06355 at 35.171 kb on + strand, within EX31_RS06355 at 35.171 kb on + strand, within EX31_RS06355 at 35.171 kb on + strand, within EX31_RS06355 at 35.172 kb on - strand, within EX31_RS06355 at 35.172 kb on - strand, within EX31_RS06355 at 35.172 kb on - strand, within EX31_RS06355 at 35.172 kb on - strand, within EX31_RS06355 at 35.172 kb on - strand, within EX31_RS06355 at 35.192 kb on + strand, within EX31_RS06355 at 35.192 kb on + strand, within EX31_RS06355 at 35.193 kb on - strand, within EX31_RS06355 at 35.436 kb on + strand, within EX31_RS06355 at 35.436 kb on + strand, within EX31_RS06355 at 35.437 kb on - strand, within EX31_RS06355 at 35.562 kb on + strand, within EX31_RS06355 at 35.585 kb on + strand at 35.586 kb on - strand at 35.586 kb on - strand at 35.679 kb on + strand at 35.691 kb on + strand at 35.708 kb on - strand at 35.710 kb on + strand at 35.710 kb on + strand at 35.710 kb on + strand at 35.710 kb on + strand at 35.711 kb on - strand at 35.711 kb on - strand at 35.724 kb on + strand at 35.724 kb on + strand at 35.724 kb on + strand at 35.725 kb on - strand at 35.725 kb on - strand at 35.758 kb on + strand at 35.761 kb on - strand at 35.761 kb on - strand at 35.794 kb on - strand at 35.828 kb on + strand at 35.864 kb on + strand at 35.864 kb on + strand at 35.865 kb on - strand at 36.025 kb on - strand, within EX31_RS06360 at 36.025 kb on - strand, within EX31_RS06360 at 36.040 kb on + strand, within EX31_RS06360 at 36.076 kb on + strand, within EX31_RS06360 at 36.076 kb on + strand, within EX31_RS06360 at 36.076 kb on + strand, within EX31_RS06360 at 36.076 kb on + strand, within EX31_RS06360 at 36.172 kb on + strand, within EX31_RS06360 at 36.242 kb on + strand, within EX31_RS06360 at 36.317 kb on + strand, within EX31_RS06360 at 36.317 kb on + strand, within EX31_RS06360 at 36.317 kb on + strand, within EX31_RS06360 at 36.318 kb on - strand, within EX31_RS06360 at 36.318 kb on - strand, within EX31_RS06360 at 36.318 kb on - strand, within EX31_RS06360 at 36.408 kb on + strand, within EX31_RS06360 at 36.425 kb on + strand, within EX31_RS06360 at 36.425 kb on + strand, within EX31_RS06360 at 36.496 kb on + strand, within EX31_RS06360 at 36.496 kb on + strand, within EX31_RS06360 at 36.496 kb on + strand, within EX31_RS06360 at 36.497 kb on - strand, within EX31_RS06360 at 36.498 kb on + strand, within EX31_RS06360 at 36.498 kb on + strand, within EX31_RS06360 at 36.498 kb on + strand, within EX31_RS06360 at 36.498 kb on + strand, within EX31_RS06360 at 36.499 kb on - strand, within EX31_RS06360 at 36.509 kb on + strand, within EX31_RS06360 at 36.509 kb on + strand, within EX31_RS06360 at 36.587 kb on - strand, within EX31_RS06360 at 36.595 kb on + strand, within EX31_RS06360 at 36.598 kb on + strand, within EX31_RS06360 at 36.646 kb on - strand, within EX31_RS06360
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); ShewMM media; no shaking remove 33,501 + EX31_RS06350 0.43 -0.2 33,627 + EX31_RS06350 0.51 +0.6 33,663 + EX31_RS06350 0.53 -0.3 33,687 + EX31_RS06350 0.55 +0.8 33,687 + EX31_RS06350 0.55 +0.9 33,688 - EX31_RS06350 0.55 +0.1 33,688 - EX31_RS06350 0.55 -0.9 33,688 - EX31_RS06350 0.55 +1.9 33,688 - EX31_RS06350 0.55 +0.7 33,688 - EX31_RS06350 0.55 +2.2 33,690 + EX31_RS06350 0.55 +0.1 33,693 + EX31_RS06350 0.55 -0.1 33,693 + EX31_RS06350 0.55 -0.2 33,693 + EX31_RS06350 0.55 +0.2 33,758 + EX31_RS06350 0.59 +0.5 33,758 + EX31_RS06350 0.59 +0.4 33,822 - EX31_RS06350 0.63 -0.6 33,963 + EX31_RS06350 0.72 -0.1 33,963 + EX31_RS06350 0.72 -1.6 33,963 + EX31_RS06350 0.72 +0.5 33,963 + EX31_RS06350 0.72 -0.2 33,964 - EX31_RS06350 0.72 +0.2 33,964 - EX31_RS06350 0.72 +0.2 33,964 - EX31_RS06350 0.72 +1.6 33,964 - EX31_RS06350 0.72 +0.1 33,965 + EX31_RS06350 0.72 -0.2 33,966 - EX31_RS06350 0.72 -0.6 34,068 + EX31_RS06350 0.78 -0.1 34,128 + EX31_RS06350 0.82 -0.2 34,129 - EX31_RS06350 0.82 +0.9 34,211 + EX31_RS06350 0.87 +0.6 34,267 - -1.0 34,286 + -1.5 34,287 - +0.6 34,318 - +2.0 34,324 - -1.6 34,363 - +0.9 34,393 - +0.8 34,407 + -0.5 34,407 + +2.2 34,407 + -0.5 34,407 + -0.2 34,426 - -0.5 34,478 + -0.6 34,513 + -0.1 34,679 + EX31_RS06355 0.16 -1.2 34,680 - EX31_RS06355 0.16 -0.2 34,716 + EX31_RS06355 0.19 +0.8 34,717 - EX31_RS06355 0.19 +0.6 34,717 - EX31_RS06355 0.19 -0.2 34,720 + EX31_RS06355 0.19 +0.4 34,720 + EX31_RS06355 0.19 -0.0 34,721 - EX31_RS06355 0.19 -0.1 34,721 - EX31_RS06355 0.19 -0.6 34,721 - EX31_RS06355 0.19 -1.5 34,721 - EX31_RS06355 0.19 +0.5 34,888 + EX31_RS06355 0.33 +0.4 34,962 + EX31_RS06355 0.39 -0.4 34,963 - EX31_RS06355 0.39 -0.2 35,040 + EX31_RS06355 0.46 -1.5 35,171 + EX31_RS06355 0.57 +0.4 35,171 + EX31_RS06355 0.57 +0.8 35,171 + EX31_RS06355 0.57 -1.0 35,171 + EX31_RS06355 0.57 +0.5 35,172 - EX31_RS06355 0.57 +0.8 35,172 - EX31_RS06355 0.57 +0.7 35,172 - EX31_RS06355 0.57 -0.6 35,172 - EX31_RS06355 0.57 -0.2 35,172 - EX31_RS06355 0.57 +0.7 35,192 + EX31_RS06355 0.58 +1.2 35,192 + EX31_RS06355 0.58 +1.1 35,193 - EX31_RS06355 0.59 +1.4 35,436 + EX31_RS06355 0.79 +0.9 35,436 + EX31_RS06355 0.79 +0.7 35,437 - EX31_RS06355 0.79 +0.2 35,562 + EX31_RS06355 0.89 +0.8 35,585 + -0.4 35,586 - +1.1 35,586 - -0.6 35,679 + -0.1 35,691 + -1.1 35,708 - +0.6 35,710 + -0.2 35,710 + +1.4 35,710 + -0.5 35,710 + +0.5 35,711 - +0.4 35,711 - -0.4 35,724 + +0.1 35,724 + -0.9 35,724 + +2.4 35,725 - +0.7 35,725 - +0.1 35,758 + -1.0 35,761 - +0.4 35,761 - -1.7 35,794 - +2.4 35,828 + -0.1 35,864 + -0.6 35,864 + +0.0 35,865 - +1.0 36,025 - EX31_RS06360 0.24 +1.8 36,025 - EX31_RS06360 0.24 +1.4 36,040 + EX31_RS06360 0.25 -2.0 36,076 + EX31_RS06360 0.29 +0.4 36,076 + EX31_RS06360 0.29 +1.4 36,076 + EX31_RS06360 0.29 -0.1 36,076 + EX31_RS06360 0.29 -1.2 36,172 + EX31_RS06360 0.39 -0.0 36,242 + EX31_RS06360 0.47 +0.5 36,317 + EX31_RS06360 0.55 -1.8 36,317 + EX31_RS06360 0.55 -0.5 36,317 + EX31_RS06360 0.55 +0.2 36,318 - EX31_RS06360 0.55 +1.0 36,318 - EX31_RS06360 0.55 +0.4 36,318 - EX31_RS06360 0.55 -0.2 36,408 + EX31_RS06360 0.64 -0.2 36,425 + EX31_RS06360 0.66 -1.0 36,425 + EX31_RS06360 0.66 -0.3 36,496 + EX31_RS06360 0.74 -2.2 36,496 + EX31_RS06360 0.74 -1.6 36,496 + EX31_RS06360 0.74 -0.7 36,497 - EX31_RS06360 0.74 +2.1 36,498 + EX31_RS06360 0.74 +0.1 36,498 + EX31_RS06360 0.74 -0.2 36,498 + EX31_RS06360 0.74 -0.4 36,498 + EX31_RS06360 0.74 -3.0 36,499 - EX31_RS06360 0.74 -2.2 36,509 + EX31_RS06360 0.75 -0.5 36,509 + EX31_RS06360 0.75 +1.0 36,587 - EX31_RS06360 0.84 -1.5 36,595 + EX31_RS06360 0.84 -0.5 36,598 + EX31_RS06360 0.85 -0.3 36,646 - EX31_RS06360 0.90 +0.2
Or see this region's nucleotide sequence