Strain Fitness in Rahnella sp. WP5 around EX31_RS02015

Experiment: L-Serine (N); ShewMM media; no shaking

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntEX31_RS02010 and EX31_RS25105 are separated by 11 nucleotidesEX31_RS25105 and EX31_RS02015 are separated by 36 nucleotidesEX31_RS02015 and EX31_RS02020 are separated by 47 nucleotides EX31_RS02010: EX31_RS02010 - cytochrome d ubiquinol oxidase subunit II, at 440,199 to 441,206 _RS02010 EX31_RS25105: EX31_RS25105 - DUF2474 domain-containing protein, at 441,218 to 441,382 _RS25105 EX31_RS02015: EX31_RS02015 - TetR family transcriptional regulator, at 441,419 to 441,997 _RS02015 EX31_RS02020: EX31_RS02020 - MFS transporter, at 442,045 to 443,541 _RS02020 Position (kb) 441 442Strain fitness (log2 ratio) -2 -1 0 1 2 3at 440.495 kb on - strand, within EX31_RS02010at 440.640 kb on + strand, within EX31_RS02010at 440.640 kb on + strand, within EX31_RS02010at 440.640 kb on + strand, within EX31_RS02010at 440.640 kb on + strand, within EX31_RS02010at 440.640 kb on + strand, within EX31_RS02010at 440.641 kb on - strand, within EX31_RS02010at 440.641 kb on - strand, within EX31_RS02010at 440.800 kb on + strand, within EX31_RS02010at 440.941 kb on - strand, within EX31_RS02010at 440.941 kb on - strand, within EX31_RS02010at 441.009 kb on + strand, within EX31_RS02010at 441.010 kb on - strand, within EX31_RS02010at 441.144 kb on + strandat 441.160 kb on - strandat 441.162 kb on + strandat 441.162 kb on + strandat 441.163 kb on - strandat 441.163 kb on - strandat 441.257 kb on - strand, within EX31_RS25105at 441.322 kb on - strand, within EX31_RS25105at 441.380 kb on + strandat 441.380 kb on + strandat 441.455 kb on - strandat 441.514 kb on - strand, within EX31_RS02015at 441.625 kb on + strand, within EX31_RS02015at 441.625 kb on + strand, within EX31_RS02015at 441.626 kb on - strand, within EX31_RS02015at 441.626 kb on - strand, within EX31_RS02015at 441.626 kb on - strand, within EX31_RS02015at 441.626 kb on - strand, within EX31_RS02015at 441.626 kb on - strand, within EX31_RS02015at 441.626 kb on - strand, within EX31_RS02015at 441.626 kb on - strand, within EX31_RS02015at 441.626 kb on - strand, within EX31_RS02015at 441.626 kb on - strand, within EX31_RS02015at 441.626 kb on - strand, within EX31_RS02015at 441.626 kb on - strand, within EX31_RS02015at 441.665 kb on - strand, within EX31_RS02015at 441.694 kb on - strand, within EX31_RS02015at 441.707 kb on - strand, within EX31_RS02015at 441.730 kb on - strand, within EX31_RS02015at 441.730 kb on - strand, within EX31_RS02015at 441.730 kb on - strand, within EX31_RS02015at 441.730 kb on - strand, within EX31_RS02015at 441.865 kb on - strand, within EX31_RS02015at 441.865 kb on - strand, within EX31_RS02015at 441.892 kb on - strand, within EX31_RS02015at 441.998 kb on - strandat 442.044 kb on - strandat 442.044 kb on - strandat 442.074 kb on + strandat 442.074 kb on + strandat 442.075 kb on - strandat 442.908 kb on - strand, within EX31_RS02020at 442.971 kb on + strand, within EX31_RS02020at 442.971 kb on + strand, within EX31_RS02020at 442.972 kb on - strand, within EX31_RS02020at 442.975 kb on - strand, within EX31_RS02020

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Serine (N); ShewMM media; no shaking
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440,495 - EX31_RS02010 0.29 +2.9
440,640 + EX31_RS02010 0.44 +0.9
440,640 + EX31_RS02010 0.44 -1.0
440,640 + EX31_RS02010 0.44 +0.4
440,640 + EX31_RS02010 0.44 -0.1
440,640 + EX31_RS02010 0.44 -1.1
440,641 - EX31_RS02010 0.44 -1.3
440,641 - EX31_RS02010 0.44 +0.3
440,800 + EX31_RS02010 0.60 +0.9
440,941 - EX31_RS02010 0.74 +1.5
440,941 - EX31_RS02010 0.74 -1.0
441,009 + EX31_RS02010 0.80 -0.1
441,010 - EX31_RS02010 0.80 -0.7
441,144 + +0.3
441,160 - +0.7
441,162 + -0.8
441,162 + -1.4
441,163 - +1.3
441,163 - +1.0
441,257 - EX31_RS25105 0.24 +0.9
441,322 - EX31_RS25105 0.63 +0.6
441,380 + +0.2
441,380 + -0.1
441,455 - -0.9
441,514 - EX31_RS02015 0.16 -0.3
441,625 + EX31_RS02015 0.36 -1.1
441,625 + EX31_RS02015 0.36 +0.4
441,626 - EX31_RS02015 0.36 -1.0
441,626 - EX31_RS02015 0.36 -0.2
441,626 - EX31_RS02015 0.36 -0.3
441,626 - EX31_RS02015 0.36 -1.0
441,626 - EX31_RS02015 0.36 +0.8
441,626 - EX31_RS02015 0.36 -0.9
441,626 - EX31_RS02015 0.36 -2.0
441,626 - EX31_RS02015 0.36 +0.4
441,626 - EX31_RS02015 0.36 -0.8
441,626 - EX31_RS02015 0.36 -0.3
441,626 - EX31_RS02015 0.36 -0.7
441,665 - EX31_RS02015 0.42 -1.7
441,694 - EX31_RS02015 0.47 -0.2
441,707 - EX31_RS02015 0.50 +0.1
441,730 - EX31_RS02015 0.54 -0.1
441,730 - EX31_RS02015 0.54 -0.5
441,730 - EX31_RS02015 0.54 -1.3
441,730 - EX31_RS02015 0.54 +1.1
441,865 - EX31_RS02015 0.77 -1.0
441,865 - EX31_RS02015 0.77 -0.7
441,892 - EX31_RS02015 0.82 -0.5
441,998 - -0.9
442,044 - +0.5
442,044 - +0.3
442,074 + -0.6
442,074 + -0.5
442,075 - -0.1
442,908 - EX31_RS02020 0.58 -2.0
442,971 + EX31_RS02020 0.62 +1.2
442,971 + EX31_RS02020 0.62 +1.3
442,972 - EX31_RS02020 0.62 -0.0
442,975 - EX31_RS02020 0.62 -0.5

Or see this region's nucleotide sequence