Experiment: L-Serine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS01785 and EX31_RS25905 are separated by 182 nucleotides EX31_RS25905 and EX31_RS01790 are separated by 42 nucleotides EX31_RS01790 and EX31_RS01795 overlap by 17 nucleotides EX31_RS01795 and EX31_RS01800 are separated by 74 nucleotides
EX31_RS01785: EX31_RS01785 - YfdQ family protein, at 382,839 to 383,666
_RS01785
EX31_RS25905: EX31_RS25905 - hypothetical protein, at 383,849 to 384,013
_RS25905
EX31_RS01790: EX31_RS01790 - excisionase, at 384,056 to 384,298
_RS01790
EX31_RS01795: EX31_RS01795 - tyrosine-type recombinase/integrase, at 384,282 to 385,406
_RS01795
EX31_RS01800: EX31_RS01800 - porin, at 385,481 to 386,152
_RS01800
Position (kb) 384
385
386 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 383.416 kb on + strand, within EX31_RS01785 at 383.416 kb on + strand, within EX31_RS01785 at 383.438 kb on + strand, within EX31_RS01785 at 383.438 kb on + strand, within EX31_RS01785 at 383.439 kb on - strand, within EX31_RS01785 at 383.460 kb on - strand, within EX31_RS01785 at 383.472 kb on + strand, within EX31_RS01785 at 383.473 kb on - strand, within EX31_RS01785 at 383.679 kb on - strand at 383.715 kb on + strand at 383.818 kb on + strand at 383.828 kb on + strand at 383.829 kb on - strand at 383.832 kb on + strand at 383.832 kb on + strand at 383.832 kb on + strand at 383.837 kb on - strand at 383.918 kb on - strand, within EX31_RS25905 at 383.985 kb on - strand, within EX31_RS25905 at 384.012 kb on - strand at 384.031 kb on + strand at 384.033 kb on + strand at 384.053 kb on + strand at 384.054 kb on - strand at 384.054 kb on - strand at 384.054 kb on - strand at 384.055 kb on + strand at 384.055 kb on + strand at 384.055 kb on + strand at 384.055 kb on + strand at 384.055 kb on + strand at 384.055 kb on + strand at 384.055 kb on + strand at 384.056 kb on - strand at 384.056 kb on - strand at 384.111 kb on + strand, within EX31_RS01790 at 384.112 kb on - strand, within EX31_RS01790 at 384.116 kb on - strand, within EX31_RS01790 at 384.137 kb on + strand, within EX31_RS01790 at 384.156 kb on + strand, within EX31_RS01790 at 384.158 kb on + strand, within EX31_RS01790 at 384.159 kb on - strand, within EX31_RS01790 at 384.251 kb on - strand, within EX31_RS01790 at 384.252 kb on + strand, within EX31_RS01790 at 384.252 kb on + strand, within EX31_RS01790 at 384.253 kb on - strand, within EX31_RS01790 at 384.253 kb on - strand, within EX31_RS01790 at 384.297 kb on - strand at 384.297 kb on - strand at 384.300 kb on + strand at 384.300 kb on + strand at 384.300 kb on + strand at 384.301 kb on - strand at 384.301 kb on - strand at 384.301 kb on - strand at 384.301 kb on - strand at 384.301 kb on - strand at 384.303 kb on - strand at 384.330 kb on + strand at 384.367 kb on - strand at 384.386 kb on + strand at 384.386 kb on + strand at 384.387 kb on - strand at 384.437 kb on - strand, within EX31_RS01795 at 384.447 kb on - strand, within EX31_RS01795 at 384.528 kb on - strand, within EX31_RS01795 at 384.546 kb on - strand, within EX31_RS01795 at 384.550 kb on - strand, within EX31_RS01795 at 384.671 kb on - strand, within EX31_RS01795 at 384.705 kb on + strand, within EX31_RS01795 at 384.754 kb on + strand, within EX31_RS01795 at 384.763 kb on + strand, within EX31_RS01795 at 384.807 kb on - strand, within EX31_RS01795 at 384.940 kb on + strand, within EX31_RS01795 at 384.965 kb on + strand, within EX31_RS01795 at 384.967 kb on + strand, within EX31_RS01795 at 384.968 kb on - strand, within EX31_RS01795 at 385.010 kb on + strand, within EX31_RS01795 at 385.012 kb on + strand, within EX31_RS01795 at 385.013 kb on - strand, within EX31_RS01795 at 385.013 kb on - strand, within EX31_RS01795 at 385.013 kb on - strand, within EX31_RS01795 at 385.039 kb on + strand, within EX31_RS01795 at 385.040 kb on - strand, within EX31_RS01795 at 385.043 kb on + strand, within EX31_RS01795 at 385.043 kb on + strand, within EX31_RS01795 at 385.044 kb on - strand, within EX31_RS01795 at 385.180 kb on + strand, within EX31_RS01795 at 385.180 kb on + strand, within EX31_RS01795 at 385.181 kb on - strand, within EX31_RS01795 at 385.184 kb on + strand, within EX31_RS01795 at 385.327 kb on + strand at 385.362 kb on + strand at 385.363 kb on - strand at 385.408 kb on + strand at 385.474 kb on + strand at 385.516 kb on + strand at 385.590 kb on + strand, within EX31_RS01800 at 385.590 kb on + strand, within EX31_RS01800 at 385.590 kb on + strand, within EX31_RS01800 at 385.591 kb on - strand, within EX31_RS01800 at 385.661 kb on + strand, within EX31_RS01800 at 385.662 kb on - strand, within EX31_RS01800 at 385.689 kb on + strand, within EX31_RS01800 at 385.705 kb on + strand, within EX31_RS01800 at 385.740 kb on + strand, within EX31_RS01800 at 385.740 kb on + strand, within EX31_RS01800 at 385.740 kb on + strand, within EX31_RS01800 at 385.740 kb on + strand, within EX31_RS01800 at 385.741 kb on - strand, within EX31_RS01800 at 385.741 kb on - strand, within EX31_RS01800 at 385.741 kb on - strand, within EX31_RS01800 at 385.808 kb on - strand, within EX31_RS01800 at 385.882 kb on + strand, within EX31_RS01800 at 385.986 kb on + strand, within EX31_RS01800 at 385.986 kb on + strand, within EX31_RS01800 at 385.987 kb on - strand, within EX31_RS01800 at 385.987 kb on - strand, within EX31_RS01800 at 385.987 kb on - strand, within EX31_RS01800 at 385.991 kb on + strand, within EX31_RS01800 at 386.075 kb on - strand, within EX31_RS01800 at 386.082 kb on - strand, within EX31_RS01800 at 386.082 kb on - strand, within EX31_RS01800
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); ShewMM media; no shaking remove 383,416 + EX31_RS01785 0.70 -1.7 383,416 + EX31_RS01785 0.70 -0.6 383,438 + EX31_RS01785 0.72 +1.1 383,438 + EX31_RS01785 0.72 +1.6 383,439 - EX31_RS01785 0.72 +0.6 383,460 - EX31_RS01785 0.75 +0.9 383,472 + EX31_RS01785 0.76 -2.0 383,473 - EX31_RS01785 0.77 +3.1 383,679 - -0.3 383,715 + -0.9 383,818 + +0.1 383,828 + +0.3 383,829 - -0.9 383,832 + -0.8 383,832 + +1.4 383,832 + +1.0 383,837 - +1.2 383,918 - EX31_RS25905 0.42 +2.4 383,985 - EX31_RS25905 0.82 +0.3 384,012 - +0.1 384,031 + +2.5 384,033 + -0.1 384,053 + +0.9 384,054 - +1.5 384,054 - +0.7 384,054 - -0.9 384,055 + +0.5 384,055 + +1.1 384,055 + -0.4 384,055 + +0.5 384,055 + +0.5 384,055 + -0.6 384,055 + -0.9 384,056 - -1.0 384,056 - +0.1 384,111 + EX31_RS01790 0.23 -0.3 384,112 - EX31_RS01790 0.23 +1.0 384,116 - EX31_RS01790 0.25 +0.1 384,137 + EX31_RS01790 0.33 +0.9 384,156 + EX31_RS01790 0.41 -0.7 384,158 + EX31_RS01790 0.42 +0.7 384,159 - EX31_RS01790 0.42 -0.4 384,251 - EX31_RS01790 0.80 -1.9 384,252 + EX31_RS01790 0.81 +0.5 384,252 + EX31_RS01790 0.81 +0.7 384,253 - EX31_RS01790 0.81 +1.1 384,253 - EX31_RS01790 0.81 +1.9 384,297 - +0.2 384,297 - +1.6 384,300 + +2.1 384,300 + +2.2 384,300 + -0.6 384,301 - +0.9 384,301 - +0.4 384,301 - +0.7 384,301 - -0.0 384,301 - -0.4 384,303 - -0.9 384,330 + +0.7 384,367 - -0.9 384,386 + +1.3 384,386 + -0.2 384,387 - -0.1 384,437 - EX31_RS01795 0.14 -0.5 384,447 - EX31_RS01795 0.15 +0.9 384,528 - EX31_RS01795 0.22 -0.6 384,546 - EX31_RS01795 0.23 -0.7 384,550 - EX31_RS01795 0.24 +0.8 384,671 - EX31_RS01795 0.35 -0.1 384,705 + EX31_RS01795 0.38 +1.3 384,754 + EX31_RS01795 0.42 +1.2 384,763 + EX31_RS01795 0.43 +1.2 384,807 - EX31_RS01795 0.47 -0.1 384,940 + EX31_RS01795 0.58 -0.2 384,965 + EX31_RS01795 0.61 +0.5 384,967 + EX31_RS01795 0.61 +1.7 384,968 - EX31_RS01795 0.61 +2.0 385,010 + EX31_RS01795 0.65 -0.3 385,012 + EX31_RS01795 0.65 +1.2 385,013 - EX31_RS01795 0.65 +0.3 385,013 - EX31_RS01795 0.65 +1.7 385,013 - EX31_RS01795 0.65 -0.9 385,039 + EX31_RS01795 0.67 +0.6 385,040 - EX31_RS01795 0.67 +1.9 385,043 + EX31_RS01795 0.68 +1.1 385,043 + EX31_RS01795 0.68 +0.9 385,044 - EX31_RS01795 0.68 -0.5 385,180 + EX31_RS01795 0.80 +0.1 385,180 + EX31_RS01795 0.80 +1.3 385,181 - EX31_RS01795 0.80 +0.5 385,184 + EX31_RS01795 0.80 +0.6 385,327 + +0.6 385,362 + -0.9 385,363 - -0.5 385,408 + -0.2 385,474 + -0.2 385,516 + +1.4 385,590 + EX31_RS01800 0.16 +0.6 385,590 + EX31_RS01800 0.16 -0.6 385,590 + EX31_RS01800 0.16 +2.1 385,591 - EX31_RS01800 0.16 +1.1 385,661 + EX31_RS01800 0.27 -0.4 385,662 - EX31_RS01800 0.27 +1.3 385,689 + EX31_RS01800 0.31 +0.9 385,705 + EX31_RS01800 0.33 +0.7 385,740 + EX31_RS01800 0.39 -0.5 385,740 + EX31_RS01800 0.39 +0.9 385,740 + EX31_RS01800 0.39 +1.9 385,740 + EX31_RS01800 0.39 +1.7 385,741 - EX31_RS01800 0.39 -0.5 385,741 - EX31_RS01800 0.39 +0.3 385,741 - EX31_RS01800 0.39 +0.9 385,808 - EX31_RS01800 0.49 -2.3 385,882 + EX31_RS01800 0.60 -0.5 385,986 + EX31_RS01800 0.75 +1.5 385,986 + EX31_RS01800 0.75 -1.5 385,987 - EX31_RS01800 0.75 -0.1 385,987 - EX31_RS01800 0.75 -1.7 385,987 - EX31_RS01800 0.75 -0.0 385,991 + EX31_RS01800 0.76 -0.4 386,075 - EX31_RS01800 0.88 +0.1 386,082 - EX31_RS01800 0.89 +3.5 386,082 - EX31_RS01800 0.89 +1.7
Or see this region's nucleotide sequence