Experiment: R2A with Tetracycline 0.0005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS20900 and CA265_RS20905 are separated by 55 nucleotides CA265_RS20905 and CA265_RS20910 are separated by 179 nucleotides CA265_RS20910 and CA265_RS20915 are separated by 70 nucleotides CA265_RS20915 and CA265_RS20920 are separated by 231 nucleotides
CA265_RS20900: CA265_RS20900 - hypoxanthine phosphoribosyltransferase, at 5,154,400 to 5,154,942
_RS20900
CA265_RS20905: CA265_RS20905 - phosphatidylserine decarboxylase, at 5,154,998 to 5,155,657
_RS20905
CA265_RS20910: CA265_RS20910 - mechanosensitive ion channel protein MscS, at 5,155,837 to 5,157,012
_RS20910
CA265_RS20915: CA265_RS20915 - 50S ribosomal protein L19, at 5,157,083 to 5,157,430
_RS20915
CA265_RS20920: CA265_RS20920 - hypothetical protein, at 5,157,662 to 5,158,151
_RS20920
Position (kb)
5155
5156
5157
5158 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5154.838 kb on + strand, within CA265_RS20900 at 5154.859 kb on - strand, within CA265_RS20900 at 5154.889 kb on + strand at 5154.923 kb on - strand at 5155.666 kb on + strand at 5155.696 kb on + strand at 5155.745 kb on + strand at 5155.765 kb on - strand at 5155.768 kb on + strand at 5155.768 kb on + strand at 5155.768 kb on + strand at 5155.783 kb on - strand at 5155.787 kb on - strand at 5155.831 kb on + strand at 5155.845 kb on + strand at 5155.846 kb on - strand at 5155.846 kb on - strand at 5155.891 kb on - strand at 5155.911 kb on + strand at 5155.997 kb on + strand, within CA265_RS20910 at 5155.997 kb on + strand, within CA265_RS20910 at 5156.018 kb on + strand, within CA265_RS20910 at 5156.018 kb on + strand, within CA265_RS20910 at 5156.019 kb on - strand, within CA265_RS20910 at 5156.022 kb on + strand, within CA265_RS20910 at 5156.022 kb on + strand, within CA265_RS20910 at 5156.029 kb on + strand, within CA265_RS20910 at 5156.059 kb on - strand, within CA265_RS20910 at 5156.106 kb on - strand, within CA265_RS20910 at 5156.247 kb on + strand, within CA265_RS20910 at 5156.248 kb on - strand, within CA265_RS20910 at 5156.281 kb on - strand, within CA265_RS20910 at 5156.281 kb on - strand, within CA265_RS20910 at 5156.346 kb on + strand, within CA265_RS20910 at 5156.380 kb on + strand, within CA265_RS20910 at 5156.381 kb on - strand, within CA265_RS20910 at 5156.383 kb on + strand, within CA265_RS20910 at 5156.458 kb on + strand, within CA265_RS20910 at 5156.458 kb on + strand, within CA265_RS20910 at 5156.520 kb on + strand, within CA265_RS20910 at 5156.562 kb on + strand, within CA265_RS20910 at 5156.583 kb on + strand, within CA265_RS20910 at 5156.586 kb on - strand, within CA265_RS20910 at 5156.615 kb on - strand, within CA265_RS20910 at 5156.626 kb on - strand, within CA265_RS20910 at 5156.727 kb on + strand, within CA265_RS20910 at 5156.738 kb on - strand, within CA265_RS20910 at 5156.762 kb on - strand, within CA265_RS20910 at 5156.792 kb on - strand at 5156.825 kb on - strand, within CA265_RS20910 at 5156.901 kb on + strand at 5156.902 kb on - strand at 5156.923 kb on + strand at 5157.023 kb on + strand at 5157.065 kb on - strand at 5157.070 kb on - strand at 5157.596 kb on - strand at 5157.664 kb on - strand at 5157.707 kb on - strand at 5157.722 kb on - strand, within CA265_RS20920 at 5157.761 kb on + strand, within CA265_RS20920 at 5157.764 kb on - strand, within CA265_RS20920 at 5157.764 kb on - strand, within CA265_RS20920 at 5157.764 kb on - strand, within CA265_RS20920 at 5157.805 kb on + strand, within CA265_RS20920 at 5157.806 kb on - strand, within CA265_RS20920 at 5157.843 kb on + strand, within CA265_RS20920 at 5157.886 kb on + strand, within CA265_RS20920 at 5157.892 kb on + strand, within CA265_RS20920 at 5157.892 kb on + strand, within CA265_RS20920 at 5157.893 kb on - strand, within CA265_RS20920 at 5157.915 kb on - strand, within CA265_RS20920 at 5157.922 kb on + strand, within CA265_RS20920
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Tetracycline 0.0005 mg/ml remove 5,154,838 + CA265_RS20900 0.81 -0.9 5,154,859 - CA265_RS20900 0.85 +0.1 5,154,889 + -0.4 5,154,923 - -0.7 5,155,666 + +0.6 5,155,696 + -0.2 5,155,745 + +0.4 5,155,765 - +0.6 5,155,768 + +0.1 5,155,768 + -1.4 5,155,768 + -0.5 5,155,783 - +0.0 5,155,787 - -0.9 5,155,831 + +0.0 5,155,845 + -1.1 5,155,846 - +1.0 5,155,846 - -0.2 5,155,891 - +0.7 5,155,911 + -0.3 5,155,997 + CA265_RS20910 0.14 +0.2 5,155,997 + CA265_RS20910 0.14 +0.8 5,156,018 + CA265_RS20910 0.15 -0.6 5,156,018 + CA265_RS20910 0.15 -0.3 5,156,019 - CA265_RS20910 0.15 -1.7 5,156,022 + CA265_RS20910 0.16 -1.1 5,156,022 + CA265_RS20910 0.16 +0.6 5,156,029 + CA265_RS20910 0.16 -0.3 5,156,059 - CA265_RS20910 0.19 -1.3 5,156,106 - CA265_RS20910 0.23 -2.6 5,156,247 + CA265_RS20910 0.35 -0.6 5,156,248 - CA265_RS20910 0.35 -1.6 5,156,281 - CA265_RS20910 0.38 -1.8 5,156,281 - CA265_RS20910 0.38 -0.4 5,156,346 + CA265_RS20910 0.43 +0.3 5,156,380 + CA265_RS20910 0.46 -1.6 5,156,381 - CA265_RS20910 0.46 +1.0 5,156,383 + CA265_RS20910 0.46 +0.0 5,156,458 + CA265_RS20910 0.53 -0.6 5,156,458 + CA265_RS20910 0.53 +0.6 5,156,520 + CA265_RS20910 0.58 +0.0 5,156,562 + CA265_RS20910 0.62 -0.1 5,156,583 + CA265_RS20910 0.63 -0.5 5,156,586 - CA265_RS20910 0.64 -0.4 5,156,615 - CA265_RS20910 0.66 -1.1 5,156,626 - CA265_RS20910 0.67 +0.2 5,156,727 + CA265_RS20910 0.76 -1.0 5,156,738 - CA265_RS20910 0.77 -0.0 5,156,762 - CA265_RS20910 0.79 -1.0 5,156,792 - +2.2 5,156,825 - CA265_RS20910 0.84 -3.3 5,156,901 + +0.2 5,156,902 - -0.8 5,156,923 + -1.2 5,157,023 + -1.5 5,157,065 - -0.3 5,157,070 - +1.3 5,157,596 - +0.4 5,157,664 - -0.2 5,157,707 - -0.5 5,157,722 - CA265_RS20920 0.12 +0.7 5,157,761 + CA265_RS20920 0.20 -0.0 5,157,764 - CA265_RS20920 0.21 +0.5 5,157,764 - CA265_RS20920 0.21 -0.5 5,157,764 - CA265_RS20920 0.21 +0.2 5,157,805 + CA265_RS20920 0.29 -0.1 5,157,806 - CA265_RS20920 0.29 -0.5 5,157,843 + CA265_RS20920 0.37 -1.2 5,157,886 + CA265_RS20920 0.46 -0.4 5,157,892 + CA265_RS20920 0.47 -0.2 5,157,892 + CA265_RS20920 0.47 -0.2 5,157,893 - CA265_RS20920 0.47 +0.4 5,157,915 - CA265_RS20920 0.52 +0.1 5,157,922 + CA265_RS20920 0.53 -0.2
Or see this region's nucleotide sequence