Experiment: reconstituted MRS with Pectin 10 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS20975 and BBR_RS16380 are separated by 70 nucleotides BBR_RS16380 and BBR_RS16385 are separated by 338 nucleotides BBR_RS16385 and BBR_RS16390 are separated by 84 nucleotides
BBR_RS20975: BBR_RS20975 - transposase, at 1,433,039 to 1,433,212
_RS20975
BBR_RS16380: BBR_RS16380 - hypothetical protein, at 1,433,283 to 1,433,489
_RS16380
BBR_RS16385: BBR_RS16385 - hypothetical protein, at 1,433,828 to 1,434,061
_RS16385
BBR_RS16390: BBR_RS16390 - Fic family protein, at 1,434,146 to 1,435,063
_RS16390
Position (kb)
1433
1434
1435 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1432.832 kb on + strand at 1432.842 kb on - strand at 1432.859 kb on + strand at 1432.968 kb on - strand at 1433.042 kb on + strand at 1433.216 kb on + strand at 1433.223 kb on + strand at 1433.257 kb on + strand at 1433.257 kb on + strand at 1433.265 kb on - strand at 1433.276 kb on + strand at 1433.285 kb on + strand at 1433.322 kb on - strand, within BBR_RS16380 at 1433.327 kb on + strand, within BBR_RS16380 at 1433.366 kb on - strand, within BBR_RS16380 at 1433.374 kb on + strand, within BBR_RS16380 at 1433.376 kb on - strand, within BBR_RS16380 at 1433.382 kb on - strand, within BBR_RS16380 at 1433.382 kb on - strand, within BBR_RS16380 at 1433.382 kb on - strand, within BBR_RS16380 at 1433.462 kb on - strand, within BBR_RS16380 at 1433.521 kb on + strand at 1433.531 kb on + strand at 1433.568 kb on + strand at 1433.573 kb on + strand at 1433.581 kb on - strand at 1433.590 kb on + strand at 1433.660 kb on - strand at 1433.677 kb on - strand at 1433.679 kb on - strand at 1433.809 kb on + strand at 1433.816 kb on + strand at 1433.826 kb on + strand at 1433.826 kb on + strand at 1433.826 kb on + strand at 1433.829 kb on - strand at 1433.841 kb on + strand at 1433.841 kb on + strand at 1433.841 kb on + strand at 1433.850 kb on + strand at 1433.898 kb on + strand, within BBR_RS16385 at 1433.898 kb on + strand, within BBR_RS16385 at 1433.898 kb on - strand, within BBR_RS16385 at 1433.898 kb on - strand, within BBR_RS16385 at 1433.906 kb on - strand, within BBR_RS16385 at 1433.906 kb on - strand, within BBR_RS16385 at 1433.906 kb on - strand, within BBR_RS16385 at 1433.966 kb on - strand, within BBR_RS16385 at 1433.970 kb on + strand, within BBR_RS16385 at 1434.072 kb on + strand at 1434.072 kb on + strand at 1434.080 kb on - strand at 1434.109 kb on - strand at 1434.128 kb on + strand at 1434.130 kb on + strand at 1434.143 kb on + strand at 1434.143 kb on + strand at 1434.143 kb on + strand at 1434.151 kb on - strand at 1434.151 kb on - strand at 1434.151 kb on - strand at 1434.151 kb on - strand at 1434.151 kb on - strand at 1434.153 kb on + strand at 1434.200 kb on + strand at 1434.248 kb on - strand, within BBR_RS16390 at 1434.248 kb on - strand, within BBR_RS16390 at 1434.284 kb on + strand, within BBR_RS16390 at 1434.342 kb on - strand, within BBR_RS16390 at 1434.429 kb on - strand, within BBR_RS16390 at 1434.492 kb on + strand, within BBR_RS16390 at 1434.533 kb on + strand, within BBR_RS16390 at 1434.576 kb on + strand, within BBR_RS16390 at 1434.584 kb on - strand, within BBR_RS16390 at 1434.599 kb on - strand, within BBR_RS16390 at 1434.666 kb on - strand, within BBR_RS16390 at 1434.693 kb on - strand, within BBR_RS16390 at 1434.740 kb on - strand, within BBR_RS16390 at 1434.835 kb on + strand, within BBR_RS16390 at 1434.843 kb on - strand, within BBR_RS16390 at 1434.859 kb on + strand, within BBR_RS16390 at 1434.877 kb on - strand, within BBR_RS16390 at 1434.890 kb on + strand, within BBR_RS16390 at 1434.898 kb on - strand, within BBR_RS16390 at 1434.913 kb on + strand, within BBR_RS16390 at 1434.919 kb on - strand, within BBR_RS16390 at 1434.921 kb on - strand, within BBR_RS16390 at 1434.934 kb on + strand, within BBR_RS16390 at 1434.939 kb on + strand, within BBR_RS16390 at 1434.958 kb on - strand, within BBR_RS16390 at 1435.000 kb on + strand at 1435.000 kb on + strand at 1435.008 kb on - strand at 1435.036 kb on - strand at 1435.046 kb on - strand at 1435.048 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Pectin 10 mg/ml remove 1,432,832 + -0.1 1,432,842 - -2.6 1,432,859 + +0.2 1,432,968 - -0.7 1,433,042 + -0.6 1,433,216 + +1.5 1,433,223 + +0.7 1,433,257 + +0.7 1,433,257 + +0.4 1,433,265 - -1.4 1,433,276 + +0.7 1,433,285 + +0.3 1,433,322 - BBR_RS16380 0.19 -1.0 1,433,327 + BBR_RS16380 0.21 -0.7 1,433,366 - BBR_RS16380 0.40 +0.8 1,433,374 + BBR_RS16380 0.44 -1.6 1,433,376 - BBR_RS16380 0.45 +0.2 1,433,382 - BBR_RS16380 0.48 +0.9 1,433,382 - BBR_RS16380 0.48 +0.1 1,433,382 - BBR_RS16380 0.48 +0.9 1,433,462 - BBR_RS16380 0.86 +0.5 1,433,521 + -0.7 1,433,531 + +1.0 1,433,568 + -1.5 1,433,573 + +0.6 1,433,581 - -0.2 1,433,590 + -0.1 1,433,660 - -0.1 1,433,677 - -0.1 1,433,679 - -0.3 1,433,809 + +0.3 1,433,816 + -0.8 1,433,826 + -0.5 1,433,826 + +1.0 1,433,826 + -0.5 1,433,829 - +2.2 1,433,841 + -0.4 1,433,841 + +0.5 1,433,841 + -0.2 1,433,850 + +1.1 1,433,898 + BBR_RS16385 0.30 +0.4 1,433,898 + BBR_RS16385 0.30 +1.2 1,433,898 - BBR_RS16385 0.30 +0.3 1,433,898 - BBR_RS16385 0.30 -1.4 1,433,906 - BBR_RS16385 0.33 +4.4 1,433,906 - BBR_RS16385 0.33 -1.4 1,433,906 - BBR_RS16385 0.33 +0.5 1,433,966 - BBR_RS16385 0.59 +1.3 1,433,970 + BBR_RS16385 0.61 +0.6 1,434,072 + -0.6 1,434,072 + +1.6 1,434,080 - -1.0 1,434,109 - +0.1 1,434,128 + +0.9 1,434,130 + -0.9 1,434,143 + -1.3 1,434,143 + -0.9 1,434,143 + +0.7 1,434,151 - +2.0 1,434,151 - +0.8 1,434,151 - -1.1 1,434,151 - +0.1 1,434,151 - +0.8 1,434,153 + -0.2 1,434,200 + -0.7 1,434,248 - BBR_RS16390 0.11 -0.9 1,434,248 - BBR_RS16390 0.11 -0.8 1,434,284 + BBR_RS16390 0.15 -0.2 1,434,342 - BBR_RS16390 0.21 -1.2 1,434,429 - BBR_RS16390 0.31 -0.2 1,434,492 + BBR_RS16390 0.38 +0.0 1,434,533 + BBR_RS16390 0.42 -0.9 1,434,576 + BBR_RS16390 0.47 +0.5 1,434,584 - BBR_RS16390 0.48 -0.4 1,434,599 - BBR_RS16390 0.49 +1.3 1,434,666 - BBR_RS16390 0.57 +0.1 1,434,693 - BBR_RS16390 0.60 +0.1 1,434,740 - BBR_RS16390 0.65 -1.2 1,434,835 + BBR_RS16390 0.75 +0.1 1,434,843 - BBR_RS16390 0.76 +1.2 1,434,859 + BBR_RS16390 0.78 +0.2 1,434,877 - BBR_RS16390 0.80 +0.8 1,434,890 + BBR_RS16390 0.81 +0.9 1,434,898 - BBR_RS16390 0.82 -0.1 1,434,913 + BBR_RS16390 0.84 +1.2 1,434,919 - BBR_RS16390 0.84 +0.3 1,434,921 - BBR_RS16390 0.84 -0.9 1,434,934 + BBR_RS16390 0.86 -0.0 1,434,939 + BBR_RS16390 0.86 -2.2 1,434,958 - BBR_RS16390 0.88 -0.2 1,435,000 + -0.2 1,435,000 + -0.0 1,435,008 - +0.9 1,435,036 - -0.1 1,435,046 - +3.8 1,435,048 + +0.1
Or see this region's nucleotide sequence