Experiment: reconstituted MRS with Pectin 10 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS13560 and BBR_RS13565 are separated by 7 nucleotides BBR_RS13565 and BBR_RS20785 are separated by 299 nucleotides BBR_RS20785 and BBR_RS13575 are separated by 39 nucleotides
BBR_RS13560: BBR_RS13560 - MATE family efflux transporter, at 814,983 to 816,341
_RS13560
BBR_RS13565: BBR_RS13565 - DNA-(apurinic or apyrimidinic site) lyase endonuclease III base excision repair, at 816,349 to 817,041
_RS13565
BBR_RS20785: BBR_RS20785 - PadR family transcriptional regulator, at 817,341 to 817,487
_RS20785
BBR_RS13575: BBR_RS13575 - sugar O-acetyltransferase, at 817,527 to 818,201
_RS13575
Position (kb)
816
817
818 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 815.392 kb on - strand, within BBR_RS13560 at 815.430 kb on - strand, within BBR_RS13560 at 815.521 kb on + strand, within BBR_RS13560 at 815.548 kb on - strand, within BBR_RS13560 at 815.614 kb on - strand, within BBR_RS13560 at 815.724 kb on - strand, within BBR_RS13560 at 815.734 kb on - strand, within BBR_RS13560 at 815.792 kb on + strand, within BBR_RS13560 at 815.868 kb on - strand, within BBR_RS13560 at 815.904 kb on - strand, within BBR_RS13560 at 815.923 kb on + strand, within BBR_RS13560 at 815.933 kb on + strand, within BBR_RS13560 at 815.942 kb on + strand, within BBR_RS13560 at 816.079 kb on - strand, within BBR_RS13560 at 816.079 kb on - strand, within BBR_RS13560 at 816.079 kb on - strand, within BBR_RS13560 at 816.090 kb on + strand, within BBR_RS13560 at 816.143 kb on + strand, within BBR_RS13560 at 816.177 kb on + strand, within BBR_RS13560 at 816.186 kb on - strand, within BBR_RS13560 at 816.186 kb on - strand, within BBR_RS13560 at 816.188 kb on + strand, within BBR_RS13560 at 816.381 kb on + strand at 816.420 kb on + strand, within BBR_RS13565 at 816.420 kb on + strand, within BBR_RS13565 at 816.447 kb on - strand, within BBR_RS13565 at 816.448 kb on - strand, within BBR_RS13565 at 816.448 kb on - strand, within BBR_RS13565 at 816.462 kb on - strand, within BBR_RS13565 at 816.462 kb on - strand, within BBR_RS13565 at 816.525 kb on - strand, within BBR_RS13565 at 816.534 kb on - strand, within BBR_RS13565 at 816.549 kb on + strand, within BBR_RS13565 at 816.567 kb on + strand, within BBR_RS13565 at 816.569 kb on - strand, within BBR_RS13565 at 816.607 kb on - strand, within BBR_RS13565 at 816.632 kb on + strand, within BBR_RS13565 at 816.640 kb on - strand, within BBR_RS13565 at 816.663 kb on + strand, within BBR_RS13565 at 816.666 kb on - strand, within BBR_RS13565 at 816.685 kb on - strand, within BBR_RS13565 at 816.687 kb on + strand, within BBR_RS13565 at 816.704 kb on - strand, within BBR_RS13565 at 816.787 kb on + strand, within BBR_RS13565 at 816.855 kb on - strand, within BBR_RS13565 at 816.894 kb on + strand, within BBR_RS13565 at 817.078 kb on + strand at 817.226 kb on + strand at 817.226 kb on + strand at 817.226 kb on + strand at 817.226 kb on + strand at 817.313 kb on - strand at 817.371 kb on - strand, within BBR_RS20785 at 817.371 kb on - strand, within BBR_RS20785 at 817.603 kb on + strand, within BBR_RS13575 at 817.700 kb on - strand, within BBR_RS13575 at 817.755 kb on + strand, within BBR_RS13575 at 817.755 kb on + strand, within BBR_RS13575 at 817.763 kb on - strand, within BBR_RS13575 at 817.793 kb on + strand, within BBR_RS13575 at 817.810 kb on - strand, within BBR_RS13575 at 817.852 kb on - strand, within BBR_RS13575 at 817.852 kb on - strand, within BBR_RS13575 at 817.927 kb on + strand, within BBR_RS13575 at 817.927 kb on + strand, within BBR_RS13575 at 817.927 kb on + strand, within BBR_RS13575 at 817.927 kb on + strand, within BBR_RS13575 at 817.927 kb on + strand, within BBR_RS13575 at 817.927 kb on + strand, within BBR_RS13575 at 817.927 kb on + strand, within BBR_RS13575 at 817.968 kb on + strand, within BBR_RS13575 at 817.998 kb on - strand, within BBR_RS13575 at 818.028 kb on + strand, within BBR_RS13575
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Pectin 10 mg/ml remove 815,392 - BBR_RS13560 0.30 +1.2 815,430 - BBR_RS13560 0.33 +1.2 815,521 + BBR_RS13560 0.40 -0.8 815,548 - BBR_RS13560 0.42 -0.9 815,614 - BBR_RS13560 0.46 -0.7 815,724 - BBR_RS13560 0.55 +0.0 815,734 - BBR_RS13560 0.55 -1.3 815,792 + BBR_RS13560 0.60 +2.2 815,868 - BBR_RS13560 0.65 +0.3 815,904 - BBR_RS13560 0.68 -0.3 815,923 + BBR_RS13560 0.69 -1.1 815,933 + BBR_RS13560 0.70 -0.1 815,942 + BBR_RS13560 0.71 +0.4 816,079 - BBR_RS13560 0.81 +0.7 816,079 - BBR_RS13560 0.81 -0.0 816,079 - BBR_RS13560 0.81 +0.3 816,090 + BBR_RS13560 0.81 -0.3 816,143 + BBR_RS13560 0.85 -0.7 816,177 + BBR_RS13560 0.88 +0.3 816,186 - BBR_RS13560 0.89 -0.8 816,186 - BBR_RS13560 0.89 -0.8 816,188 + BBR_RS13560 0.89 -0.6 816,381 + -0.4 816,420 + BBR_RS13565 0.10 +1.0 816,420 + BBR_RS13565 0.10 +0.2 816,447 - BBR_RS13565 0.14 +1.8 816,448 - BBR_RS13565 0.14 +0.3 816,448 - BBR_RS13565 0.14 +1.0 816,462 - BBR_RS13565 0.16 -0.4 816,462 - BBR_RS13565 0.16 +0.7 816,525 - BBR_RS13565 0.25 +0.0 816,534 - BBR_RS13565 0.27 +0.7 816,549 + BBR_RS13565 0.29 +0.6 816,567 + BBR_RS13565 0.31 +0.0 816,569 - BBR_RS13565 0.32 -1.2 816,607 - BBR_RS13565 0.37 -0.0 816,632 + BBR_RS13565 0.41 +1.5 816,640 - BBR_RS13565 0.42 +0.9 816,663 + BBR_RS13565 0.45 +1.0 816,666 - BBR_RS13565 0.46 +0.7 816,685 - BBR_RS13565 0.48 +0.3 816,687 + BBR_RS13565 0.49 +0.2 816,704 - BBR_RS13565 0.51 +1.0 816,787 + BBR_RS13565 0.63 +1.1 816,855 - BBR_RS13565 0.73 -0.6 816,894 + BBR_RS13565 0.79 +0.4 817,078 + -2.0 817,226 + +0.1 817,226 + -1.1 817,226 + +0.0 817,226 + -1.1 817,313 - +1.5 817,371 - BBR_RS20785 0.20 -0.0 817,371 - BBR_RS20785 0.20 +0.3 817,603 + BBR_RS13575 0.11 +0.2 817,700 - BBR_RS13575 0.26 +0.7 817,755 + BBR_RS13575 0.34 -0.3 817,755 + BBR_RS13575 0.34 +0.6 817,763 - BBR_RS13575 0.35 +1.7 817,793 + BBR_RS13575 0.39 -0.4 817,810 - BBR_RS13575 0.42 +0.3 817,852 - BBR_RS13575 0.48 -0.4 817,852 - BBR_RS13575 0.48 +0.2 817,927 + BBR_RS13575 0.59 +0.1 817,927 + BBR_RS13575 0.59 +0.1 817,927 + BBR_RS13575 0.59 -0.2 817,927 + BBR_RS13575 0.59 +1.7 817,927 + BBR_RS13575 0.59 -0.7 817,927 + BBR_RS13575 0.59 +0.8 817,927 + BBR_RS13575 0.59 -2.0 817,968 + BBR_RS13575 0.65 +1.1 817,998 - BBR_RS13575 0.70 -0.5 818,028 + BBR_RS13575 0.74 +0.1
Or see this region's nucleotide sequence