Experiment: succinate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_3016 and Ac3H11_3017 are separated by 54 nucleotides Ac3H11_3017 and Ac3H11_3018 are separated by 29 nucleotides Ac3H11_3018 and Ac3H11_3019 are separated by 27 nucleotides
Ac3H11_3016 - Methylcrotonyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.4), at 37,755 to 39,782
_3016
Ac3H11_3017 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 39,837 to 40,766
_3017
Ac3H11_3018 - Hydroxymethylglutaryl-CoA lyase (EC 4.1.3.4), at 40,796 to 41,710
_3018
Ac3H11_3019 - Uncharacterized protein SCO3165, at 41,738 to 42,247
_3019
Position (kb) 39
40
41 Strain fitness (log2 ratio) -2
-1
0
1 at 39.867 kb on - strand at 39.963 kb on + strand, within Ac3H11_3017 at 39.963 kb on + strand, within Ac3H11_3017 at 40.131 kb on + strand, within Ac3H11_3017 at 40.131 kb on + strand, within Ac3H11_3017 at 40.131 kb on + strand, within Ac3H11_3017 at 40.132 kb on - strand, within Ac3H11_3017 at 40.488 kb on + strand, within Ac3H11_3017 at 40.488 kb on + strand, within Ac3H11_3017 at 40.488 kb on + strand, within Ac3H11_3017 at 40.488 kb on + strand, within Ac3H11_3017 at 40.488 kb on + strand, within Ac3H11_3017 at 40.489 kb on - strand, within Ac3H11_3017 at 40.489 kb on - strand, within Ac3H11_3017 at 40.762 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction succinate (C) remove 39,867 - +0.3 39,963 + Ac3H11_3017 0.14 -0.4 39,963 + Ac3H11_3017 0.14 -1.3 40,131 + Ac3H11_3017 0.32 -1.0 40,131 + Ac3H11_3017 0.32 -1.1 40,131 + Ac3H11_3017 0.32 -0.5 40,132 - Ac3H11_3017 0.32 +0.2 40,488 + Ac3H11_3017 0.70 -0.7 40,488 + Ac3H11_3017 0.70 -2.2 40,488 + Ac3H11_3017 0.70 -0.5 40,488 + Ac3H11_3017 0.70 -1.0 40,488 + Ac3H11_3017 0.70 -0.8 40,489 - Ac3H11_3017 0.70 +0.8 40,489 - Ac3H11_3017 0.70 +0.5 40,762 - -2.0
Or see this region's nucleotide sequence