Experiment: Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse1; 10 generations
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02105 and MMP_RS02110 are separated by 15 nucleotides MMP_RS02110 and MMP_RS02115 are separated by 241 nucleotides MMP_RS02115 and MMP_RS02120 are separated by 201 nucleotides
MMP_RS02105: MMP_RS02105 - uroporphyrinogen-III synthase, at 391,835 to 392,569
_RS02105
MMP_RS02110: MMP_RS02110 - hypothetical protein, at 392,585 to 392,863
_RS02110
MMP_RS02115: MMP_RS02115 - phosphopyruvate hydratase, at 393,105 to 394,397
_RS02115
MMP_RS02120: MMP_RS02120 - alanine--tRNA ligase, at 394,599 to 397,277
_RS02120
Position (kb) 393
394
395 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 392.169 kb on + strand at 392.433 kb on - strand at 392.616 kb on - strand, within MMP_RS02110 at 392.616 kb on - strand, within MMP_RS02110 at 392.876 kb on - strand at 392.896 kb on + strand at 392.910 kb on + strand at 392.920 kb on + strand at 393.010 kb on - strand at 393.515 kb on - strand at 393.541 kb on + strand at 393.926 kb on + strand at 393.937 kb on + strand at 394.320 kb on - strand at 394.401 kb on - strand at 394.406 kb on + strand at 394.443 kb on - strand at 394.453 kb on + strand at 394.479 kb on + strand at 394.491 kb on - strand at 394.577 kb on + strand at 394.620 kb on + strand at 394.779 kb on - strand at 394.780 kb on + strand at 395.033 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse1; 10 generations remove 392,169 + -1.8 392,433 - -4.4 392,616 - MMP_RS02110 0.11 -2.9 392,616 - MMP_RS02110 0.11 -1.5 392,876 - -4.5 392,896 + -3.5 392,910 + -6.5 392,920 + -1.5 393,010 - -1.0 393,515 - -2.4 393,541 + -1.0 393,926 + -2.0 393,937 + -3.1 394,320 - -3.7 394,401 - +0.9 394,406 + +0.2 394,443 - +0.3 394,453 + +0.1 394,479 + +0.1 394,491 - +0.6 394,577 + -3.1 394,620 + -2.5 394,779 - -4.0 394,780 + -3.6 395,033 - -4.0
Or see this region's nucleotide sequence