Experiment: L-Serine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS24175 and EX31_RS24180 are separated by 223 nucleotides EX31_RS24180 and EX31_RS24185 are separated by 84 nucleotides EX31_RS24185 and EX31_RS24190 are separated by 4 nucleotides EX31_RS24190 and EX31_RS24195 are separated by 177 nucleotides
EX31_RS24175: EX31_RS24175 - hypothetical protein, at 605,239 to 605,505
_RS24175
EX31_RS24180: EX31_RS24180 - serine/threonine protein phosphatase, at 605,729 to 606,388
_RS24180
EX31_RS24185: EX31_RS24185 - DNA-binding transcriptional repressor DeoR, at 606,473 to 607,237
_RS24185
EX31_RS24190: EX31_RS24190 - DNA gyrase inhibitor, at 607,242 to 607,718
_RS24190
EX31_RS24195: EX31_RS24195 - membrane protein, at 607,896 to 608,405
_RS24195
Position (kb) 606
607
608 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 605.489 kb on - strand at 605.525 kb on - strand at 605.542 kb on - strand at 605.562 kb on - strand at 605.580 kb on + strand at 605.639 kb on + strand at 605.640 kb on - strand at 605.687 kb on + strand at 605.687 kb on + strand at 605.688 kb on - strand at 605.688 kb on - strand at 605.693 kb on + strand at 605.693 kb on + strand at 605.698 kb on + strand at 605.699 kb on - strand at 605.728 kb on + strand at 605.744 kb on + strand at 605.745 kb on - strand at 605.768 kb on + strand at 605.769 kb on - strand at 605.769 kb on - strand at 605.769 kb on - strand at 605.769 kb on - strand at 605.769 kb on - strand at 605.769 kb on - strand at 605.780 kb on + strand at 605.780 kb on + strand at 605.780 kb on + strand at 605.781 kb on - strand at 605.782 kb on + strand at 605.782 kb on + strand at 605.783 kb on - strand at 605.783 kb on - strand at 605.814 kb on + strand, within EX31_RS24180 at 605.814 kb on + strand, within EX31_RS24180 at 605.814 kb on + strand, within EX31_RS24180 at 605.814 kb on + strand, within EX31_RS24180 at 605.815 kb on - strand, within EX31_RS24180 at 605.868 kb on + strand, within EX31_RS24180 at 605.868 kb on + strand, within EX31_RS24180 at 605.868 kb on + strand, within EX31_RS24180 at 605.868 kb on + strand, within EX31_RS24180 at 605.869 kb on - strand, within EX31_RS24180 at 606.012 kb on + strand, within EX31_RS24180 at 606.012 kb on + strand, within EX31_RS24180 at 606.016 kb on + strand, within EX31_RS24180 at 606.017 kb on - strand, within EX31_RS24180 at 606.035 kb on + strand, within EX31_RS24180 at 606.036 kb on - strand, within EX31_RS24180 at 606.104 kb on - strand, within EX31_RS24180 at 606.158 kb on + strand, within EX31_RS24180 at 606.159 kb on - strand, within EX31_RS24180 at 606.159 kb on - strand, within EX31_RS24180 at 606.173 kb on + strand, within EX31_RS24180 at 606.174 kb on - strand, within EX31_RS24180 at 606.174 kb on - strand, within EX31_RS24180 at 606.174 kb on - strand, within EX31_RS24180 at 606.208 kb on + strand, within EX31_RS24180 at 606.332 kb on + strand at 606.334 kb on + strand at 606.335 kb on - strand at 606.351 kb on + strand at 606.352 kb on - strand at 606.373 kb on + strand at 606.373 kb on + strand at 606.386 kb on + strand at 606.431 kb on + strand at 606.431 kb on + strand at 606.433 kb on + strand at 606.481 kb on + strand at 606.519 kb on + strand at 606.544 kb on + strand at 606.635 kb on + strand, within EX31_RS24185 at 606.637 kb on + strand, within EX31_RS24185 at 606.637 kb on + strand, within EX31_RS24185 at 606.637 kb on + strand, within EX31_RS24185 at 606.637 kb on + strand, within EX31_RS24185 at 606.638 kb on - strand, within EX31_RS24185 at 606.674 kb on + strand, within EX31_RS24185 at 606.679 kb on + strand, within EX31_RS24185 at 606.680 kb on - strand, within EX31_RS24185 at 606.798 kb on + strand, within EX31_RS24185 at 606.854 kb on - strand, within EX31_RS24185 at 606.913 kb on + strand, within EX31_RS24185 at 606.928 kb on + strand, within EX31_RS24185 at 606.934 kb on + strand, within EX31_RS24185 at 607.068 kb on + strand, within EX31_RS24185 at 607.156 kb on + strand, within EX31_RS24185 at 607.157 kb on - strand, within EX31_RS24185 at 607.193 kb on + strand at 607.193 kb on + strand at 607.193 kb on + strand at 607.193 kb on + strand at 607.194 kb on - strand at 607.194 kb on - strand at 607.194 kb on - strand at 607.194 kb on - strand at 607.194 kb on - strand at 607.194 kb on - strand at 607.235 kb on + strand at 607.321 kb on + strand, within EX31_RS24190 at 607.321 kb on + strand, within EX31_RS24190 at 607.321 kb on + strand, within EX31_RS24190 at 607.322 kb on - strand, within EX31_RS24190 at 607.322 kb on - strand, within EX31_RS24190 at 607.355 kb on - strand, within EX31_RS24190 at 607.381 kb on + strand, within EX31_RS24190 at 607.381 kb on + strand, within EX31_RS24190 at 607.381 kb on + strand, within EX31_RS24190 at 607.442 kb on - strand, within EX31_RS24190 at 607.442 kb on - strand, within EX31_RS24190 at 607.701 kb on + strand at 607.701 kb on + strand at 607.701 kb on + strand at 607.702 kb on - strand at 607.707 kb on + strand at 607.707 kb on + strand at 607.707 kb on + strand at 607.707 kb on + strand at 607.708 kb on - strand at 607.708 kb on - strand at 607.708 kb on - strand at 607.708 kb on - strand at 607.708 kb on - strand at 607.708 kb on - strand at 607.786 kb on - strand at 607.828 kb on + strand at 607.830 kb on + strand at 607.851 kb on + strand at 607.851 kb on + strand at 607.852 kb on - strand at 607.852 kb on - strand at 607.855 kb on + strand at 607.855 kb on + strand at 607.855 kb on + strand at 607.869 kb on + strand at 607.869 kb on + strand at 607.869 kb on + strand at 607.870 kb on - strand at 607.870 kb on - strand at 607.871 kb on + strand at 607.871 kb on + strand at 607.871 kb on + strand at 607.872 kb on - strand at 607.872 kb on - strand at 607.872 kb on - strand at 607.872 kb on - strand at 607.890 kb on + strand at 607.890 kb on + strand at 607.890 kb on + strand at 607.891 kb on - strand at 607.891 kb on - strand at 607.891 kb on - strand at 607.903 kb on + strand at 607.904 kb on - strand at 607.915 kb on + strand at 607.916 kb on - strand at 607.964 kb on + strand, within EX31_RS24195 at 607.964 kb on + strand, within EX31_RS24195 at 607.993 kb on + strand, within EX31_RS24195 at 607.993 kb on + strand, within EX31_RS24195 at 607.993 kb on + strand, within EX31_RS24195 at 607.993 kb on + strand, within EX31_RS24195 at 607.994 kb on - strand, within EX31_RS24195 at 608.008 kb on + strand, within EX31_RS24195 at 608.008 kb on + strand, within EX31_RS24195 at 608.008 kb on + strand, within EX31_RS24195 at 608.009 kb on - strand, within EX31_RS24195 at 608.018 kb on + strand, within EX31_RS24195 at 608.031 kb on + strand, within EX31_RS24195 at 608.032 kb on - strand, within EX31_RS24195 at 608.034 kb on - strand, within EX31_RS24195 at 608.034 kb on - strand, within EX31_RS24195 at 608.055 kb on + strand, within EX31_RS24195 at 608.055 kb on + strand, within EX31_RS24195 at 608.055 kb on + strand, within EX31_RS24195 at 608.055 kb on + strand, within EX31_RS24195 at 608.055 kb on + strand, within EX31_RS24195 at 608.056 kb on - strand, within EX31_RS24195 at 608.056 kb on - strand, within EX31_RS24195 at 608.067 kb on + strand, within EX31_RS24195 at 608.067 kb on + strand, within EX31_RS24195 at 608.067 kb on + strand, within EX31_RS24195 at 608.067 kb on + strand, within EX31_RS24195 at 608.067 kb on + strand, within EX31_RS24195 at 608.067 kb on + strand, within EX31_RS24195 at 608.068 kb on - strand, within EX31_RS24195 at 608.068 kb on - strand, within EX31_RS24195 at 608.068 kb on - strand, within EX31_RS24195 at 608.068 kb on - strand, within EX31_RS24195 at 608.068 kb on - strand, within EX31_RS24195 at 608.068 kb on - strand, within EX31_RS24195 at 608.068 kb on - strand, within EX31_RS24195 at 608.069 kb on + strand, within EX31_RS24195 at 608.069 kb on + strand, within EX31_RS24195 at 608.069 kb on + strand, within EX31_RS24195 at 608.069 kb on + strand, within EX31_RS24195 at 608.069 kb on + strand, within EX31_RS24195 at 608.069 kb on + strand, within EX31_RS24195 at 608.070 kb on - strand, within EX31_RS24195 at 608.070 kb on - strand, within EX31_RS24195 at 608.070 kb on - strand, within EX31_RS24195 at 608.070 kb on - strand, within EX31_RS24195 at 608.070 kb on - strand, within EX31_RS24195 at 608.096 kb on + strand, within EX31_RS24195 at 608.097 kb on - strand, within EX31_RS24195 at 608.097 kb on - strand, within EX31_RS24195 at 608.114 kb on + strand, within EX31_RS24195 at 608.114 kb on + strand, within EX31_RS24195 at 608.114 kb on + strand, within EX31_RS24195 at 608.115 kb on - strand, within EX31_RS24195 at 608.142 kb on - strand, within EX31_RS24195 at 608.142 kb on - strand, within EX31_RS24195 at 608.182 kb on + strand, within EX31_RS24195 at 608.182 kb on + strand, within EX31_RS24195 at 608.193 kb on + strand, within EX31_RS24195 at 608.193 kb on + strand, within EX31_RS24195 at 608.193 kb on + strand, within EX31_RS24195 at 608.194 kb on - strand, within EX31_RS24195 at 608.194 kb on - strand, within EX31_RS24195 at 608.194 kb on - strand, within EX31_RS24195 at 608.195 kb on + strand, within EX31_RS24195 at 608.196 kb on - strand, within EX31_RS24195
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; no shaking remove 605,489 - +0.6 605,525 - +0.9 605,542 - +0.5 605,562 - +0.1 605,580 + -2.2 605,639 + +0.6 605,640 - -1.2 605,687 + -0.1 605,687 + +0.7 605,688 - -0.1 605,688 - +0.4 605,693 + -0.3 605,693 + +0.5 605,698 + -0.3 605,699 - +0.8 605,728 + +0.3 605,744 + -0.5 605,745 - -0.8 605,768 + -0.3 605,769 - +0.8 605,769 - +1.9 605,769 - +0.6 605,769 - +0.7 605,769 - -1.4 605,769 - -0.5 605,780 + +0.6 605,780 + -1.4 605,780 + -0.9 605,781 - +1.1 605,782 + -1.3 605,782 + -0.9 605,783 - +1.3 605,783 - -1.2 605,814 + EX31_RS24180 0.13 -0.8 605,814 + EX31_RS24180 0.13 -0.4 605,814 + EX31_RS24180 0.13 +2.5 605,814 + EX31_RS24180 0.13 -1.3 605,815 - EX31_RS24180 0.13 +0.5 605,868 + EX31_RS24180 0.21 +1.0 605,868 + EX31_RS24180 0.21 +0.7 605,868 + EX31_RS24180 0.21 +0.5 605,868 + EX31_RS24180 0.21 -0.4 605,869 - EX31_RS24180 0.21 +0.4 606,012 + EX31_RS24180 0.43 -0.9 606,012 + EX31_RS24180 0.43 -1.6 606,016 + EX31_RS24180 0.43 -0.1 606,017 - EX31_RS24180 0.44 -1.3 606,035 + EX31_RS24180 0.46 +1.9 606,036 - EX31_RS24180 0.47 +0.2 606,104 - EX31_RS24180 0.57 +2.5 606,158 + EX31_RS24180 0.65 +0.1 606,159 - EX31_RS24180 0.65 +0.3 606,159 - EX31_RS24180 0.65 -2.0 606,173 + EX31_RS24180 0.67 +0.2 606,174 - EX31_RS24180 0.67 -0.3 606,174 - EX31_RS24180 0.67 -0.1 606,174 - EX31_RS24180 0.67 +1.4 606,208 + EX31_RS24180 0.73 +0.2 606,332 + -0.9 606,334 + +0.6 606,335 - +0.3 606,351 + -2.2 606,352 - -1.6 606,373 + -0.4 606,373 + +2.2 606,386 + +0.8 606,431 + -2.3 606,431 + +0.3 606,433 + -0.2 606,481 + +0.7 606,519 + -0.5 606,544 + -0.2 606,635 + EX31_RS24185 0.21 +0.4 606,637 + EX31_RS24185 0.21 +1.9 606,637 + EX31_RS24185 0.21 -0.5 606,637 + EX31_RS24185 0.21 -2.1 606,637 + EX31_RS24185 0.21 +0.1 606,638 - EX31_RS24185 0.22 +1.1 606,674 + EX31_RS24185 0.26 +1.9 606,679 + EX31_RS24185 0.27 +0.6 606,680 - EX31_RS24185 0.27 +0.9 606,798 + EX31_RS24185 0.42 -1.9 606,854 - EX31_RS24185 0.50 +0.9 606,913 + EX31_RS24185 0.58 -1.6 606,928 + EX31_RS24185 0.59 -2.0 606,934 + EX31_RS24185 0.60 -0.4 607,068 + EX31_RS24185 0.78 +1.3 607,156 + EX31_RS24185 0.89 -0.3 607,157 - EX31_RS24185 0.89 +0.4 607,193 + -0.0 607,193 + +1.3 607,193 + +1.1 607,193 + -0.8 607,194 - +0.3 607,194 - -1.4 607,194 - -0.4 607,194 - +0.3 607,194 - +1.4 607,194 - +0.4 607,235 + +0.2 607,321 + EX31_RS24190 0.17 +1.5 607,321 + EX31_RS24190 0.17 +0.2 607,321 + EX31_RS24190 0.17 -0.4 607,322 - EX31_RS24190 0.17 +0.9 607,322 - EX31_RS24190 0.17 +1.6 607,355 - EX31_RS24190 0.24 -0.7 607,381 + EX31_RS24190 0.29 +0.9 607,381 + EX31_RS24190 0.29 -1.4 607,381 + EX31_RS24190 0.29 +0.4 607,442 - EX31_RS24190 0.42 +1.8 607,442 - EX31_RS24190 0.42 +0.1 607,701 + -0.8 607,701 + -0.3 607,701 + +1.4 607,702 - -1.4 607,707 + -0.2 607,707 + -1.1 607,707 + +1.2 607,707 + +0.8 607,708 - +1.3 607,708 - +0.9 607,708 - +0.1 607,708 - -0.8 607,708 - +0.6 607,708 - +0.8 607,786 - -0.7 607,828 + -0.4 607,830 + +1.2 607,851 + -1.2 607,851 + +0.8 607,852 - -0.5 607,852 - -1.8 607,855 + -1.2 607,855 + +0.0 607,855 + -0.5 607,869 + -0.6 607,869 + -0.5 607,869 + +0.7 607,870 - -0.9 607,870 - +0.1 607,871 + -0.1 607,871 + +0.8 607,871 + +0.3 607,872 - +0.9 607,872 - -0.3 607,872 - +0.5 607,872 - -3.0 607,890 + +0.8 607,890 + -1.5 607,890 + +0.5 607,891 - -1.3 607,891 - +0.4 607,891 - -0.9 607,903 + +0.6 607,904 - +0.7 607,915 + -0.5 607,916 - +2.4 607,964 + EX31_RS24195 0.13 +0.0 607,964 + EX31_RS24195 0.13 +0.7 607,993 + EX31_RS24195 0.19 -1.4 607,993 + EX31_RS24195 0.19 -1.5 607,993 + EX31_RS24195 0.19 -0.1 607,993 + EX31_RS24195 0.19 +0.4 607,994 - EX31_RS24195 0.19 -0.3 608,008 + EX31_RS24195 0.22 +1.6 608,008 + EX31_RS24195 0.22 +0.5 608,008 + EX31_RS24195 0.22 +0.2 608,009 - EX31_RS24195 0.22 +0.1 608,018 + EX31_RS24195 0.24 +3.8 608,031 + EX31_RS24195 0.26 +0.2 608,032 - EX31_RS24195 0.27 +2.3 608,034 - EX31_RS24195 0.27 +0.6 608,034 - EX31_RS24195 0.27 -0.7 608,055 + EX31_RS24195 0.31 +0.1 608,055 + EX31_RS24195 0.31 +0.4 608,055 + EX31_RS24195 0.31 +1.9 608,055 + EX31_RS24195 0.31 +0.6 608,055 + EX31_RS24195 0.31 +1.9 608,056 - EX31_RS24195 0.31 +0.4 608,056 - EX31_RS24195 0.31 +0.1 608,067 + EX31_RS24195 0.34 +0.2 608,067 + EX31_RS24195 0.34 -0.7 608,067 + EX31_RS24195 0.34 +1.7 608,067 + EX31_RS24195 0.34 +0.0 608,067 + EX31_RS24195 0.34 -0.2 608,067 + EX31_RS24195 0.34 +0.3 608,068 - EX31_RS24195 0.34 -0.7 608,068 - EX31_RS24195 0.34 -1.8 608,068 - EX31_RS24195 0.34 +1.9 608,068 - EX31_RS24195 0.34 +0.2 608,068 - EX31_RS24195 0.34 -1.8 608,068 - EX31_RS24195 0.34 +1.9 608,068 - EX31_RS24195 0.34 -1.5 608,069 + EX31_RS24195 0.34 +1.1 608,069 + EX31_RS24195 0.34 +0.8 608,069 + EX31_RS24195 0.34 +1.2 608,069 + EX31_RS24195 0.34 -0.3 608,069 + EX31_RS24195 0.34 +1.1 608,069 + EX31_RS24195 0.34 -2.0 608,070 - EX31_RS24195 0.34 -0.5 608,070 - EX31_RS24195 0.34 +0.0 608,070 - EX31_RS24195 0.34 +1.5 608,070 - EX31_RS24195 0.34 +0.5 608,070 - EX31_RS24195 0.34 -0.2 608,096 + EX31_RS24195 0.39 -1.4 608,097 - EX31_RS24195 0.39 +0.3 608,097 - EX31_RS24195 0.39 -0.2 608,114 + EX31_RS24195 0.43 +0.1 608,114 + EX31_RS24195 0.43 +0.5 608,114 + EX31_RS24195 0.43 -0.2 608,115 - EX31_RS24195 0.43 +0.8 608,142 - EX31_RS24195 0.48 -0.0 608,142 - EX31_RS24195 0.48 +0.2 608,182 + EX31_RS24195 0.56 +0.0 608,182 + EX31_RS24195 0.56 +0.3 608,193 + EX31_RS24195 0.58 -0.0 608,193 + EX31_RS24195 0.58 +1.0 608,193 + EX31_RS24195 0.58 -0.1 608,194 - EX31_RS24195 0.58 +0.3 608,194 - EX31_RS24195 0.58 +2.6 608,194 - EX31_RS24195 0.58 +1.6 608,195 + EX31_RS24195 0.59 +1.3 608,196 - EX31_RS24195 0.59 +1.2
Or see this region's nucleotide sequence