Strain Fitness in Rahnella sp. WP5 around EX31_RS23455

Experiment: L-Serine (N); RCH2 media; no shaking

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntEX31_RS23450 and EX31_RS23455 overlap by 1 nucleotidesEX31_RS23455 and EX31_RS23460 are separated by 723 nucleotides EX31_RS23450: EX31_RS23450 - ADP-forming succinate--CoA ligase subunit beta, at 458,552 to 459,718 _RS23450 EX31_RS23455: EX31_RS23455 - succinate--CoA ligase subunit alpha, at 459,718 to 460,590 _RS23455 EX31_RS23460: EX31_RS23460 - cytochrome ubiquinol oxidase subunit I, at 461,314 to 462,882 _RS23460 Position (kb) 459 460 461Strain fitness (log2 ratio) -2 -1 0 1 2 3at 458.802 kb on - strand, within EX31_RS23450at 458.929 kb on + strand, within EX31_RS23450at 458.929 kb on + strand, within EX31_RS23450at 458.929 kb on + strand, within EX31_RS23450at 458.930 kb on - strand, within EX31_RS23450at 459.305 kb on + strand, within EX31_RS23450at 459.305 kb on + strand, within EX31_RS23450at 459.306 kb on - strand, within EX31_RS23450at 459.306 kb on - strand, within EX31_RS23450at 459.604 kb on + strandat 459.750 kb on + strandat 459.750 kb on + strandat 459.899 kb on + strand, within EX31_RS23455at 459.906 kb on + strand, within EX31_RS23455at 459.932 kb on - strand, within EX31_RS23455at 460.002 kb on + strand, within EX31_RS23455at 460.098 kb on + strand, within EX31_RS23455at 460.192 kb on + strand, within EX31_RS23455at 460.193 kb on - strand, within EX31_RS23455at 460.236 kb on + strand, within EX31_RS23455at 460.275 kb on + strand, within EX31_RS23455at 460.378 kb on + strand, within EX31_RS23455at 460.378 kb on + strand, within EX31_RS23455at 460.414 kb on + strand, within EX31_RS23455at 460.414 kb on + strand, within EX31_RS23455at 460.449 kb on + strand, within EX31_RS23455at 460.449 kb on + strand, within EX31_RS23455at 460.592 kb on + strandat 460.592 kb on + strandat 460.592 kb on + strandat 460.592 kb on + strandat 460.592 kb on + strandat 460.593 kb on - strandat 460.593 kb on - strandat 460.595 kb on + strandat 460.618 kb on + strandat 460.641 kb on - strandat 460.641 kb on - strandat 460.644 kb on + strandat 460.694 kb on + strandat 460.728 kb on - strandat 460.729 kb on + strandat 460.730 kb on - strandat 460.733 kb on + strandat 460.733 kb on + strandat 460.734 kb on - strandat 460.737 kb on - strandat 460.779 kb on + strandat 460.780 kb on - strandat 460.821 kb on - strandat 460.821 kb on - strandat 460.920 kb on - strandat 460.926 kb on - strandat 460.937 kb on + strandat 460.938 kb on - strandat 460.943 kb on + strandat 460.943 kb on + strandat 461.000 kb on + strandat 461.000 kb on + strandat 461.001 kb on - strandat 461.001 kb on - strandat 461.001 kb on - strandat 461.034 kb on + strandat 461.038 kb on + strandat 461.039 kb on - strandat 461.059 kb on + strandat 461.064 kb on + strandat 461.064 kb on + strandat 461.064 kb on + strandat 461.064 kb on + strandat 461.064 kb on + strandat 461.064 kb on + strandat 461.064 kb on + strandat 461.065 kb on - strandat 461.093 kb on - strandat 461.188 kb on + strandat 461.188 kb on + strandat 461.231 kb on + strandat 461.237 kb on + strandat 461.238 kb on - strandat 461.238 kb on - strandat 461.238 kb on - strandat 461.366 kb on - strandat 461.366 kb on - strandat 461.366 kb on - strandat 461.375 kb on + strandat 461.431 kb on + strandat 461.432 kb on - strandat 461.455 kb on + strandat 461.463 kb on + strandat 461.463 kb on + strandat 461.463 kb on + strandat 461.463 kb on + strandat 461.464 kb on - strandat 461.496 kb on + strand, within EX31_RS23460at 461.497 kb on - strand, within EX31_RS23460at 461.560 kb on + strand, within EX31_RS23460at 461.560 kb on + strand, within EX31_RS23460at 461.561 kb on - strand, within EX31_RS23460at 461.572 kb on + strand, within EX31_RS23460at 461.572 kb on + strand, within EX31_RS23460at 461.572 kb on + strand, within EX31_RS23460at 461.573 kb on - strand, within EX31_RS23460at 461.573 kb on - strand, within EX31_RS23460at 461.573 kb on - strand, within EX31_RS23460at 461.584 kb on - strand, within EX31_RS23460

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; no shaking
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458,802 - EX31_RS23450 0.21 -1.2
458,929 + EX31_RS23450 0.32 -0.3
458,929 + EX31_RS23450 0.32 -1.4
458,929 + EX31_RS23450 0.32 -0.3
458,930 - EX31_RS23450 0.32 -0.8
459,305 + EX31_RS23450 0.65 +0.2
459,305 + EX31_RS23450 0.65 -0.8
459,306 - EX31_RS23450 0.65 +0.4
459,306 - EX31_RS23450 0.65 -0.7
459,604 + -0.7
459,750 + +0.4
459,750 + -0.0
459,899 + EX31_RS23455 0.21 -1.0
459,906 + EX31_RS23455 0.22 +1.0
459,932 - EX31_RS23455 0.25 -0.9
460,002 + EX31_RS23455 0.33 -1.4
460,098 + EX31_RS23455 0.44 -1.6
460,192 + EX31_RS23455 0.54 +1.0
460,193 - EX31_RS23455 0.54 +0.4
460,236 + EX31_RS23455 0.59 -0.4
460,275 + EX31_RS23455 0.64 +0.6
460,378 + EX31_RS23455 0.76 -1.2
460,378 + EX31_RS23455 0.76 -1.2
460,414 + EX31_RS23455 0.80 +1.3
460,414 + EX31_RS23455 0.80 -0.4
460,449 + EX31_RS23455 0.84 -2.3
460,449 + EX31_RS23455 0.84 -1.4
460,592 + +0.9
460,592 + +1.0
460,592 + +0.3
460,592 + +1.0
460,592 + +0.7
460,593 - -0.4
460,593 - +1.3
460,595 + +0.1
460,618 + -1.2
460,641 - +0.3
460,641 - +0.6
460,644 + +2.0
460,694 + +2.0
460,728 - +2.4
460,729 + +0.8
460,730 - +2.0
460,733 + -1.3
460,733 + +1.0
460,734 - +0.2
460,737 - +1.4
460,779 + +0.2
460,780 - +0.7
460,821 - +3.5
460,821 - -1.7
460,920 - -0.2
460,926 - -0.3
460,937 + -1.8
460,938 - +0.6
460,943 + +2.0
460,943 + +0.4
461,000 + +0.8
461,000 + -0.6
461,001 - +0.3
461,001 - +0.4
461,001 - +0.5
461,034 + +0.2
461,038 + +0.5
461,039 - +0.4
461,059 + +2.0
461,064 + +0.5
461,064 + -0.4
461,064 + -2.1
461,064 + +1.5
461,064 + +0.1
461,064 + -0.6
461,064 + -0.6
461,065 - -2.0
461,093 - +1.2
461,188 + -0.1
461,188 + -1.7
461,231 + -0.2
461,237 + -0.3
461,238 - +0.2
461,238 - +0.6
461,238 - +0.1
461,366 - +1.4
461,366 - -0.5
461,366 - -0.0
461,375 + +0.5
461,431 + -1.0
461,432 - -2.0
461,455 + +0.1
461,463 + +0.2
461,463 + -0.0
461,463 + -2.0
461,463 + -0.3
461,464 - +0.6
461,496 + EX31_RS23460 0.12 -1.4
461,497 - EX31_RS23460 0.12 +0.6
461,560 + EX31_RS23460 0.16 -0.2
461,560 + EX31_RS23460 0.16 -0.4
461,561 - EX31_RS23460 0.16 +2.5
461,572 + EX31_RS23460 0.16 -0.4
461,572 + EX31_RS23460 0.16 -2.1
461,572 + EX31_RS23460 0.16 -0.4
461,573 - EX31_RS23460 0.17 +1.1
461,573 - EX31_RS23460 0.17 -1.0
461,573 - EX31_RS23460 0.17 -0.0
461,584 - EX31_RS23460 0.17 +1.0

Or see this region's nucleotide sequence