Experiment: L-Serine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS22895 and EX31_RS22900 are separated by 5 nucleotides EX31_RS22900 and EX31_RS22905 are separated by 212 nucleotides EX31_RS22905 and EX31_RS22910 are separated by 466 nucleotides
EX31_RS22895: EX31_RS22895 - hypothetical protein, at 334,143 to 334,775
_RS22895
EX31_RS22900: EX31_RS22900 - WYL domain-containing protein, at 334,781 to 335,486
_RS22900
EX31_RS22905: EX31_RS22905 - GTPase family protein, at 335,699 to 336,574
_RS22905
EX31_RS22910: EX31_RS22910 - AAA family ATPase, at 337,041 to 338,792
_RS22910
Position (kb) 335
336
337 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 334.757 kb on + strand at 334.758 kb on - strand at 334.782 kb on + strand at 334.782 kb on + strand at 334.796 kb on + strand at 334.796 kb on + strand at 334.796 kb on + strand at 334.797 kb on - strand at 334.797 kb on - strand at 334.798 kb on + strand at 334.799 kb on - strand at 334.895 kb on + strand, within EX31_RS22900 at 334.895 kb on + strand, within EX31_RS22900 at 334.895 kb on + strand, within EX31_RS22900 at 334.895 kb on + strand, within EX31_RS22900 at 334.895 kb on + strand, within EX31_RS22900 at 334.896 kb on - strand, within EX31_RS22900 at 334.896 kb on - strand, within EX31_RS22900 at 334.896 kb on - strand, within EX31_RS22900 at 334.897 kb on + strand, within EX31_RS22900 at 334.898 kb on - strand, within EX31_RS22900 at 334.989 kb on + strand, within EX31_RS22900 at 334.989 kb on + strand, within EX31_RS22900 at 334.990 kb on - strand, within EX31_RS22900 at 335.057 kb on + strand, within EX31_RS22900 at 335.312 kb on + strand, within EX31_RS22900 at 335.322 kb on + strand, within EX31_RS22900 at 335.323 kb on - strand, within EX31_RS22900 at 335.323 kb on - strand, within EX31_RS22900 at 335.323 kb on - strand, within EX31_RS22900 at 335.323 kb on - strand, within EX31_RS22900 at 335.323 kb on - strand, within EX31_RS22900 at 335.323 kb on - strand, within EX31_RS22900 at 335.323 kb on - strand, within EX31_RS22900 at 335.432 kb on + strand at 335.433 kb on - strand at 335.433 kb on - strand at 335.436 kb on + strand at 335.575 kb on + strand at 335.576 kb on - strand at 335.643 kb on + strand at 335.643 kb on + strand at 335.643 kb on + strand at 335.643 kb on + strand at 335.643 kb on + strand at 335.643 kb on + strand at 335.644 kb on - strand at 335.644 kb on - strand at 335.645 kb on + strand at 335.655 kb on + strand at 335.656 kb on - strand at 335.667 kb on + strand at 335.667 kb on + strand at 335.667 kb on + strand at 335.667 kb on + strand at 335.667 kb on + strand at 335.667 kb on + strand at 335.667 kb on + strand at 335.667 kb on + strand at 335.667 kb on + strand at 335.668 kb on - strand at 335.789 kb on - strand, within EX31_RS22905 at 335.877 kb on - strand, within EX31_RS22905 at 335.957 kb on + strand, within EX31_RS22905 at 336.043 kb on + strand, within EX31_RS22905 at 336.043 kb on + strand, within EX31_RS22905 at 336.043 kb on + strand, within EX31_RS22905 at 336.044 kb on - strand, within EX31_RS22905 at 336.044 kb on - strand, within EX31_RS22905 at 336.047 kb on + strand, within EX31_RS22905 at 336.047 kb on + strand, within EX31_RS22905 at 336.048 kb on - strand, within EX31_RS22905 at 336.048 kb on - strand, within EX31_RS22905 at 336.048 kb on - strand, within EX31_RS22905 at 336.048 kb on - strand, within EX31_RS22905 at 336.224 kb on + strand, within EX31_RS22905 at 336.225 kb on - strand, within EX31_RS22905 at 336.265 kb on - strand, within EX31_RS22905 at 336.293 kb on + strand, within EX31_RS22905 at 336.293 kb on + strand, within EX31_RS22905 at 336.293 kb on + strand, within EX31_RS22905 at 336.293 kb on + strand, within EX31_RS22905 at 336.293 kb on + strand, within EX31_RS22905 at 336.293 kb on + strand, within EX31_RS22905 at 336.293 kb on + strand, within EX31_RS22905 at 336.294 kb on - strand, within EX31_RS22905 at 336.294 kb on - strand, within EX31_RS22905 at 336.294 kb on - strand, within EX31_RS22905 at 336.294 kb on - strand, within EX31_RS22905 at 336.302 kb on + strand, within EX31_RS22905 at 336.314 kb on + strand, within EX31_RS22905 at 336.322 kb on + strand, within EX31_RS22905 at 336.322 kb on + strand, within EX31_RS22905 at 336.323 kb on - strand, within EX31_RS22905 at 336.353 kb on + strand, within EX31_RS22905 at 336.353 kb on + strand, within EX31_RS22905 at 336.354 kb on - strand, within EX31_RS22905 at 336.451 kb on + strand, within EX31_RS22905 at 336.472 kb on - strand, within EX31_RS22905 at 336.490 kb on + strand at 336.490 kb on + strand at 336.491 kb on - strand at 336.491 kb on - strand at 336.502 kb on + strand at 336.502 kb on + strand at 336.503 kb on - strand at 336.641 kb on + strand at 336.644 kb on + strand at 336.644 kb on + strand at 336.645 kb on - strand at 336.645 kb on - strand at 336.648 kb on + strand at 336.649 kb on - strand at 336.688 kb on - strand at 336.697 kb on - strand at 336.705 kb on + strand at 336.706 kb on - strand at 336.747 kb on + strand at 336.747 kb on + strand at 336.748 kb on - strand at 336.748 kb on - strand at 336.748 kb on - strand at 336.748 kb on - strand at 336.758 kb on + strand at 336.759 kb on - strand at 336.827 kb on + strand at 336.827 kb on + strand at 336.828 kb on - strand at 336.831 kb on - strand at 336.831 kb on - strand at 336.836 kb on - strand at 336.910 kb on - strand at 336.916 kb on + strand at 336.916 kb on + strand at 336.916 kb on + strand at 336.924 kb on + strand at 336.925 kb on - strand at 336.925 kb on - strand at 336.961 kb on + strand at 336.961 kb on + strand at 336.961 kb on + strand at 336.961 kb on + strand at 336.962 kb on - strand at 336.963 kb on + strand at 336.963 kb on + strand at 336.963 kb on + strand at 336.964 kb on - strand at 336.964 kb on - strand at 336.964 kb on - strand at 336.967 kb on - strand at 336.971 kb on + strand at 336.972 kb on - strand at 337.000 kb on + strand at 337.013 kb on + strand at 337.030 kb on + strand at 337.040 kb on + strand at 337.041 kb on - strand at 337.048 kb on + strand at 337.048 kb on + strand at 337.048 kb on + strand at 337.048 kb on + strand at 337.114 kb on + strand at 337.114 kb on + strand at 337.114 kb on + strand at 337.115 kb on - strand at 337.121 kb on - strand at 337.228 kb on + strand, within EX31_RS22910 at 337.228 kb on + strand, within EX31_RS22910 at 337.228 kb on + strand, within EX31_RS22910 at 337.228 kb on + strand, within EX31_RS22910 at 337.229 kb on - strand, within EX31_RS22910 at 337.229 kb on - strand, within EX31_RS22910 at 337.282 kb on + strand, within EX31_RS22910 at 337.282 kb on + strand, within EX31_RS22910 at 337.303 kb on + strand, within EX31_RS22910 at 337.303 kb on + strand, within EX31_RS22910 at 337.303 kb on + strand, within EX31_RS22910 at 337.303 kb on + strand, within EX31_RS22910 at 337.303 kb on + strand, within EX31_RS22910 at 337.304 kb on - strand, within EX31_RS22910 at 337.304 kb on - strand, within EX31_RS22910 at 337.304 kb on - strand, within EX31_RS22910 at 337.304 kb on - strand, within EX31_RS22910 at 337.304 kb on - strand, within EX31_RS22910 at 337.328 kb on + strand, within EX31_RS22910 at 337.344 kb on - strand, within EX31_RS22910 at 337.359 kb on + strand, within EX31_RS22910 at 337.391 kb on + strand, within EX31_RS22910 at 337.391 kb on + strand, within EX31_RS22910 at 337.391 kb on + strand, within EX31_RS22910 at 337.392 kb on - strand, within EX31_RS22910 at 337.398 kb on - strand, within EX31_RS22910 at 337.424 kb on + strand, within EX31_RS22910 at 337.424 kb on + strand, within EX31_RS22910 at 337.424 kb on + strand, within EX31_RS22910 at 337.467 kb on + strand, within EX31_RS22910 at 337.467 kb on + strand, within EX31_RS22910 at 337.505 kb on + strand, within EX31_RS22910 at 337.505 kb on + strand, within EX31_RS22910 at 337.506 kb on - strand, within EX31_RS22910 at 337.508 kb on + strand, within EX31_RS22910 at 337.508 kb on + strand, within EX31_RS22910 at 337.508 kb on + strand, within EX31_RS22910 at 337.508 kb on + strand, within EX31_RS22910
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; no shaking remove 334,757 + +0.3 334,758 - +0.3 334,782 + +0.1 334,782 + +1.6 334,796 + +1.4 334,796 + -0.4 334,796 + +0.3 334,797 - +1.0 334,797 - +0.6 334,798 + +1.8 334,799 - -0.5 334,895 + EX31_RS22900 0.16 +1.4 334,895 + EX31_RS22900 0.16 +0.4 334,895 + EX31_RS22900 0.16 -0.7 334,895 + EX31_RS22900 0.16 -1.3 334,895 + EX31_RS22900 0.16 -0.6 334,896 - EX31_RS22900 0.16 +0.4 334,896 - EX31_RS22900 0.16 +1.2 334,896 - EX31_RS22900 0.16 +1.7 334,897 + EX31_RS22900 0.16 +0.5 334,898 - EX31_RS22900 0.17 -2.2 334,989 + EX31_RS22900 0.29 -0.2 334,989 + EX31_RS22900 0.29 +0.5 334,990 - EX31_RS22900 0.30 +1.7 335,057 + EX31_RS22900 0.39 +0.0 335,312 + EX31_RS22900 0.75 -1.1 335,322 + EX31_RS22900 0.77 +1.5 335,323 - EX31_RS22900 0.77 +0.2 335,323 - EX31_RS22900 0.77 -0.0 335,323 - EX31_RS22900 0.77 +1.4 335,323 - EX31_RS22900 0.77 +0.4 335,323 - EX31_RS22900 0.77 -0.5 335,323 - EX31_RS22900 0.77 -1.4 335,323 - EX31_RS22900 0.77 -0.2 335,432 + +0.8 335,433 - +0.4 335,433 - +0.5 335,436 + +0.4 335,575 + +0.7 335,576 - -1.6 335,643 + +1.7 335,643 + +1.6 335,643 + +0.8 335,643 + +0.5 335,643 + +0.4 335,643 + -0.3 335,644 - +0.4 335,644 - -0.2 335,645 + -0.8 335,655 + +1.3 335,656 - -1.6 335,667 + -0.7 335,667 + +0.6 335,667 + -0.1 335,667 + +0.4 335,667 + +0.1 335,667 + +1.6 335,667 + -0.7 335,667 + +0.3 335,667 + +1.7 335,668 - -2.5 335,789 - EX31_RS22905 0.10 -0.8 335,877 - EX31_RS22905 0.20 +0.0 335,957 + EX31_RS22905 0.29 +0.9 336,043 + EX31_RS22905 0.39 -0.1 336,043 + EX31_RS22905 0.39 -0.3 336,043 + EX31_RS22905 0.39 +1.2 336,044 - EX31_RS22905 0.39 +1.1 336,044 - EX31_RS22905 0.39 -0.7 336,047 + EX31_RS22905 0.40 -1.3 336,047 + EX31_RS22905 0.40 +0.5 336,048 - EX31_RS22905 0.40 +1.6 336,048 - EX31_RS22905 0.40 +0.2 336,048 - EX31_RS22905 0.40 +0.4 336,048 - EX31_RS22905 0.40 +0.6 336,224 + EX31_RS22905 0.60 -0.6 336,225 - EX31_RS22905 0.60 -1.6 336,265 - EX31_RS22905 0.65 +0.9 336,293 + EX31_RS22905 0.68 -0.5 336,293 + EX31_RS22905 0.68 +2.8 336,293 + EX31_RS22905 0.68 +0.1 336,293 + EX31_RS22905 0.68 +0.3 336,293 + EX31_RS22905 0.68 +1.9 336,293 + EX31_RS22905 0.68 -0.3 336,293 + EX31_RS22905 0.68 -1.0 336,294 - EX31_RS22905 0.68 +2.3 336,294 - EX31_RS22905 0.68 +0.8 336,294 - EX31_RS22905 0.68 -0.6 336,294 - EX31_RS22905 0.68 +0.0 336,302 + EX31_RS22905 0.69 +0.3 336,314 + EX31_RS22905 0.70 -1.7 336,322 + EX31_RS22905 0.71 +0.6 336,322 + EX31_RS22905 0.71 -0.4 336,323 - EX31_RS22905 0.71 -0.2 336,353 + EX31_RS22905 0.75 -0.1 336,353 + EX31_RS22905 0.75 +2.6 336,354 - EX31_RS22905 0.75 -1.6 336,451 + EX31_RS22905 0.86 +1.5 336,472 - EX31_RS22905 0.88 +0.5 336,490 + +1.0 336,490 + -1.9 336,491 - +0.4 336,491 - +1.0 336,502 + -1.2 336,502 + -0.5 336,503 - +1.0 336,641 + -1.7 336,644 + +0.9 336,644 + -0.3 336,645 - +0.7 336,645 - -0.3 336,648 + +0.7 336,649 - -0.2 336,688 - -0.6 336,697 - -0.4 336,705 + -0.3 336,706 - -2.5 336,747 + +0.1 336,747 + +1.4 336,748 - +0.9 336,748 - -0.2 336,748 - +0.5 336,748 - -0.0 336,758 + -1.5 336,759 - +1.1 336,827 + +1.0 336,827 + -1.3 336,828 - +1.0 336,831 - +0.3 336,831 - +2.0 336,836 - +1.1 336,910 - -1.0 336,916 + -0.6 336,916 + -0.7 336,916 + +0.0 336,924 + -1.3 336,925 - +1.1 336,925 - -0.3 336,961 + -1.0 336,961 + -0.7 336,961 + -1.0 336,961 + +0.4 336,962 - +1.4 336,963 + +0.2 336,963 + +0.4 336,963 + -2.0 336,964 - +0.4 336,964 - -2.6 336,964 - +1.6 336,967 - -1.8 336,971 + +0.1 336,972 - -0.5 337,000 + -0.4 337,013 + +1.5 337,030 + +0.9 337,040 + -0.6 337,041 - +1.0 337,048 + +0.8 337,048 + +0.0 337,048 + +0.2 337,048 + +1.1 337,114 + -0.2 337,114 + -0.0 337,114 + +0.3 337,115 - -0.1 337,121 - +3.1 337,228 + EX31_RS22910 0.11 -1.9 337,228 + EX31_RS22910 0.11 +0.7 337,228 + EX31_RS22910 0.11 -0.0 337,228 + EX31_RS22910 0.11 +0.8 337,229 - EX31_RS22910 0.11 -1.2 337,229 - EX31_RS22910 0.11 +1.2 337,282 + EX31_RS22910 0.14 -0.5 337,282 + EX31_RS22910 0.14 +0.0 337,303 + EX31_RS22910 0.15 -1.5 337,303 + EX31_RS22910 0.15 +0.3 337,303 + EX31_RS22910 0.15 +0.4 337,303 + EX31_RS22910 0.15 -0.3 337,303 + EX31_RS22910 0.15 -0.3 337,304 - EX31_RS22910 0.15 +2.0 337,304 - EX31_RS22910 0.15 +0.0 337,304 - EX31_RS22910 0.15 -0.3 337,304 - EX31_RS22910 0.15 +1.2 337,304 - EX31_RS22910 0.15 -0.1 337,328 + EX31_RS22910 0.16 +0.2 337,344 - EX31_RS22910 0.17 +0.4 337,359 + EX31_RS22910 0.18 +1.0 337,391 + EX31_RS22910 0.20 -0.9 337,391 + EX31_RS22910 0.20 +0.7 337,391 + EX31_RS22910 0.20 -1.2 337,392 - EX31_RS22910 0.20 +0.2 337,398 - EX31_RS22910 0.20 +0.2 337,424 + EX31_RS22910 0.22 -1.5 337,424 + EX31_RS22910 0.22 -0.4 337,424 + EX31_RS22910 0.22 +1.0 337,467 + EX31_RS22910 0.24 -0.4 337,467 + EX31_RS22910 0.24 +0.6 337,505 + EX31_RS22910 0.26 +0.9 337,505 + EX31_RS22910 0.26 +0.4 337,506 - EX31_RS22910 0.27 +1.2 337,508 + EX31_RS22910 0.27 -0.8 337,508 + EX31_RS22910 0.27 +0.4 337,508 + EX31_RS22910 0.27 -0.9 337,508 + EX31_RS22910 0.27 -1.6
Or see this region's nucleotide sequence