Experiment: L-Serine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS20065 and EX31_RS20070 are separated by 159 nucleotides EX31_RS20070 and EX31_RS20075 are separated by 71 nucleotides
EX31_RS20065: EX31_RS20065 - L-glyceraldehyde 3-phosphate reductase, at 643,615 to 644,604
_RS20065
EX31_RS20070: EX31_RS20070 - DUF2502 domain-containing protein, at 644,764 to 645,177
_RS20070
EX31_RS20075: EX31_RS20075 - Mn(2+) uptake NRAMP transporter MntH, at 645,249 to 646,478
_RS20075
Position (kb) 644
645
646 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 643.806 kb on + strand, within EX31_RS20065 at 643.806 kb on + strand, within EX31_RS20065 at 643.806 kb on + strand, within EX31_RS20065 at 643.807 kb on - strand, within EX31_RS20065 at 643.810 kb on + strand, within EX31_RS20065 at 643.810 kb on + strand, within EX31_RS20065 at 643.810 kb on + strand, within EX31_RS20065 at 643.811 kb on - strand, within EX31_RS20065 at 643.869 kb on - strand, within EX31_RS20065 at 644.005 kb on + strand, within EX31_RS20065 at 644.006 kb on - strand, within EX31_RS20065 at 644.014 kb on - strand, within EX31_RS20065 at 644.020 kb on + strand, within EX31_RS20065 at 644.020 kb on + strand, within EX31_RS20065 at 644.020 kb on + strand, within EX31_RS20065 at 644.021 kb on - strand, within EX31_RS20065 at 644.043 kb on + strand, within EX31_RS20065 at 644.044 kb on - strand, within EX31_RS20065 at 644.044 kb on - strand, within EX31_RS20065 at 644.105 kb on - strand, within EX31_RS20065 at 644.105 kb on - strand, within EX31_RS20065 at 644.200 kb on + strand, within EX31_RS20065 at 644.200 kb on + strand, within EX31_RS20065 at 644.242 kb on - strand, within EX31_RS20065 at 644.268 kb on + strand, within EX31_RS20065 at 644.268 kb on + strand, within EX31_RS20065 at 644.356 kb on - strand, within EX31_RS20065 at 644.418 kb on + strand, within EX31_RS20065 at 644.419 kb on - strand, within EX31_RS20065 at 644.419 kb on - strand, within EX31_RS20065 at 644.419 kb on - strand, within EX31_RS20065 at 644.602 kb on + strand at 644.603 kb on - strand at 644.622 kb on + strand at 644.672 kb on - strand at 644.672 kb on - strand at 644.693 kb on + strand at 644.693 kb on + strand at 644.693 kb on + strand at 644.693 kb on + strand at 644.693 kb on + strand at 644.693 kb on + strand at 644.693 kb on + strand at 644.694 kb on - strand at 644.694 kb on - strand at 644.694 kb on - strand at 644.694 kb on - strand at 644.694 kb on - strand at 644.694 kb on - strand at 644.700 kb on + strand at 644.703 kb on + strand at 644.703 kb on + strand at 644.704 kb on - strand at 644.723 kb on + strand at 644.723 kb on + strand at 644.724 kb on - strand at 644.725 kb on + strand at 644.725 kb on + strand at 644.725 kb on + strand at 644.725 kb on + strand at 644.725 kb on + strand at 644.725 kb on + strand at 644.725 kb on + strand at 644.725 kb on + strand at 644.725 kb on + strand at 644.725 kb on + strand at 644.725 kb on + strand at 644.725 kb on + strand at 644.725 kb on + strand at 644.726 kb on - strand at 644.726 kb on - strand at 644.726 kb on - strand at 644.726 kb on - strand at 644.726 kb on - strand at 644.726 kb on - strand at 644.738 kb on - strand at 644.738 kb on - strand at 644.738 kb on - strand at 644.742 kb on + strand at 644.742 kb on + strand at 644.743 kb on - strand at 644.745 kb on - strand at 644.769 kb on - strand at 644.800 kb on - strand at 644.853 kb on + strand, within EX31_RS20070 at 644.868 kb on + strand, within EX31_RS20070 at 644.868 kb on + strand, within EX31_RS20070 at 644.869 kb on - strand, within EX31_RS20070 at 644.869 kb on - strand, within EX31_RS20070 at 644.869 kb on - strand, within EX31_RS20070 at 644.869 kb on - strand, within EX31_RS20070 at 644.899 kb on + strand, within EX31_RS20070 at 644.902 kb on + strand, within EX31_RS20070 at 644.902 kb on + strand, within EX31_RS20070 at 644.903 kb on - strand, within EX31_RS20070 at 644.925 kb on + strand, within EX31_RS20070 at 644.962 kb on + strand, within EX31_RS20070 at 644.963 kb on - strand, within EX31_RS20070 at 644.963 kb on - strand, within EX31_RS20070 at 644.963 kb on - strand, within EX31_RS20070 at 644.986 kb on + strand, within EX31_RS20070 at 644.990 kb on - strand, within EX31_RS20070 at 645.100 kb on + strand, within EX31_RS20070 at 645.100 kb on + strand, within EX31_RS20070 at 645.100 kb on + strand, within EX31_RS20070 at 645.100 kb on + strand, within EX31_RS20070 at 645.101 kb on - strand, within EX31_RS20070 at 645.101 kb on - strand, within EX31_RS20070 at 645.101 kb on - strand, within EX31_RS20070 at 645.176 kb on - strand at 645.187 kb on + strand at 645.188 kb on - strand at 645.225 kb on + strand at 645.225 kb on + strand at 645.228 kb on + strand at 645.228 kb on + strand at 645.228 kb on + strand at 645.251 kb on - strand at 645.260 kb on + strand at 645.260 kb on + strand at 645.260 kb on + strand at 645.260 kb on + strand at 645.260 kb on + strand at 645.260 kb on + strand at 645.261 kb on - strand at 645.261 kb on - strand at 645.261 kb on - strand at 645.261 kb on - strand at 645.261 kb on - strand at 645.275 kb on - strand at 645.282 kb on - strand at 645.332 kb on + strand at 645.335 kb on + strand at 645.335 kb on + strand at 645.421 kb on - strand, within EX31_RS20075 at 645.454 kb on - strand, within EX31_RS20075 at 645.454 kb on - strand, within EX31_RS20075 at 645.454 kb on - strand, within EX31_RS20075 at 645.454 kb on - strand, within EX31_RS20075 at 645.473 kb on + strand, within EX31_RS20075 at 645.474 kb on - strand, within EX31_RS20075 at 645.474 kb on - strand, within EX31_RS20075 at 645.497 kb on + strand, within EX31_RS20075 at 645.498 kb on - strand, within EX31_RS20075 at 645.498 kb on - strand, within EX31_RS20075 at 645.520 kb on + strand, within EX31_RS20075 at 645.520 kb on + strand, within EX31_RS20075 at 645.521 kb on - strand, within EX31_RS20075 at 645.546 kb on + strand, within EX31_RS20075 at 645.547 kb on - strand, within EX31_RS20075 at 645.575 kb on + strand, within EX31_RS20075 at 645.575 kb on + strand, within EX31_RS20075 at 645.575 kb on + strand, within EX31_RS20075 at 645.575 kb on + strand, within EX31_RS20075 at 645.576 kb on - strand, within EX31_RS20075 at 645.576 kb on - strand, within EX31_RS20075 at 645.576 kb on - strand, within EX31_RS20075 at 645.576 kb on - strand, within EX31_RS20075 at 645.576 kb on - strand, within EX31_RS20075 at 645.576 kb on - strand, within EX31_RS20075 at 645.576 kb on - strand, within EX31_RS20075 at 645.576 kb on - strand, within EX31_RS20075 at 645.576 kb on - strand, within EX31_RS20075 at 645.576 kb on - strand, within EX31_RS20075 at 645.582 kb on + strand, within EX31_RS20075 at 645.655 kb on + strand, within EX31_RS20075 at 645.656 kb on - strand, within EX31_RS20075 at 645.656 kb on - strand, within EX31_RS20075 at 645.787 kb on + strand, within EX31_RS20075 at 645.841 kb on + strand, within EX31_RS20075 at 645.841 kb on + strand, within EX31_RS20075 at 645.842 kb on - strand, within EX31_RS20075 at 645.842 kb on - strand, within EX31_RS20075 at 645.857 kb on + strand, within EX31_RS20075 at 645.889 kb on + strand, within EX31_RS20075 at 645.889 kb on + strand, within EX31_RS20075 at 645.889 kb on + strand, within EX31_RS20075 at 645.890 kb on - strand, within EX31_RS20075 at 645.922 kb on - strand, within EX31_RS20075 at 645.977 kb on + strand, within EX31_RS20075 at 645.977 kb on + strand, within EX31_RS20075 at 645.977 kb on + strand, within EX31_RS20075 at 645.978 kb on - strand, within EX31_RS20075 at 645.978 kb on - strand, within EX31_RS20075 at 645.978 kb on - strand, within EX31_RS20075 at 645.978 kb on - strand, within EX31_RS20075 at 645.978 kb on - strand, within EX31_RS20075 at 645.978 kb on - strand, within EX31_RS20075 at 645.978 kb on - strand, within EX31_RS20075 at 645.978 kb on - strand, within EX31_RS20075 at 645.979 kb on + strand, within EX31_RS20075 at 645.979 kb on + strand, within EX31_RS20075 at 645.980 kb on - strand, within EX31_RS20075 at 645.980 kb on - strand, within EX31_RS20075 at 645.980 kb on - strand, within EX31_RS20075 at 645.980 kb on - strand, within EX31_RS20075 at 645.980 kb on - strand, within EX31_RS20075 at 646.058 kb on + strand, within EX31_RS20075 at 646.061 kb on + strand, within EX31_RS20075 at 646.062 kb on - strand, within EX31_RS20075 at 646.079 kb on + strand, within EX31_RS20075 at 646.083 kb on + strand, within EX31_RS20075 at 646.083 kb on + strand, within EX31_RS20075 at 646.083 kb on + strand, within EX31_RS20075 at 646.083 kb on + strand, within EX31_RS20075 at 646.083 kb on + strand, within EX31_RS20075 at 646.084 kb on - strand, within EX31_RS20075 at 646.084 kb on - strand, within EX31_RS20075 at 646.095 kb on + strand, within EX31_RS20075 at 646.096 kb on - strand, within EX31_RS20075 at 646.143 kb on - strand, within EX31_RS20075
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; no shaking remove 643,806 + EX31_RS20065 0.19 +2.6 643,806 + EX31_RS20065 0.19 -0.3 643,806 + EX31_RS20065 0.19 -0.8 643,807 - EX31_RS20065 0.19 -1.0 643,810 + EX31_RS20065 0.20 +0.1 643,810 + EX31_RS20065 0.20 -1.5 643,810 + EX31_RS20065 0.20 -0.1 643,811 - EX31_RS20065 0.20 +0.6 643,869 - EX31_RS20065 0.26 -1.0 644,005 + EX31_RS20065 0.39 +0.4 644,006 - EX31_RS20065 0.39 -0.6 644,014 - EX31_RS20065 0.40 -0.8 644,020 + EX31_RS20065 0.41 -0.3 644,020 + EX31_RS20065 0.41 -0.1 644,020 + EX31_RS20065 0.41 +0.5 644,021 - EX31_RS20065 0.41 +0.1 644,043 + EX31_RS20065 0.43 -0.9 644,044 - EX31_RS20065 0.43 -1.3 644,044 - EX31_RS20065 0.43 -2.6 644,105 - EX31_RS20065 0.49 +3.8 644,105 - EX31_RS20065 0.49 -0.9 644,200 + EX31_RS20065 0.59 +0.0 644,200 + EX31_RS20065 0.59 -1.5 644,242 - EX31_RS20065 0.63 +1.3 644,268 + EX31_RS20065 0.66 -0.8 644,268 + EX31_RS20065 0.66 +0.2 644,356 - EX31_RS20065 0.75 +0.2 644,418 + EX31_RS20065 0.81 +1.3 644,419 - EX31_RS20065 0.81 -0.6 644,419 - EX31_RS20065 0.81 +1.6 644,419 - EX31_RS20065 0.81 +0.2 644,602 + +0.5 644,603 - +0.8 644,622 + -0.0 644,672 - +0.2 644,672 - +1.0 644,693 + +0.9 644,693 + +0.7 644,693 + +0.1 644,693 + +0.1 644,693 + +0.4 644,693 + -0.1 644,693 + +0.9 644,694 - +0.6 644,694 - +1.6 644,694 - +0.1 644,694 - +1.5 644,694 - -0.5 644,694 - +0.2 644,700 + +0.3 644,703 + -0.3 644,703 + +0.5 644,704 - -0.2 644,723 + +1.6 644,723 + -0.4 644,724 - +0.4 644,725 + -0.8 644,725 + -1.0 644,725 + -0.4 644,725 + -0.3 644,725 + +1.2 644,725 + +0.8 644,725 + -0.1 644,725 + +0.6 644,725 + +0.2 644,725 + +0.8 644,725 + +1.1 644,725 + +0.4 644,725 + -0.1 644,726 - -1.2 644,726 - -0.2 644,726 - -0.1 644,726 - -0.4 644,726 - -0.6 644,726 - +0.3 644,738 - -0.5 644,738 - -0.1 644,738 - +0.6 644,742 + -0.7 644,742 + -1.5 644,743 - -2.0 644,745 - +0.4 644,769 - +0.2 644,800 - +0.5 644,853 + EX31_RS20070 0.21 +1.1 644,868 + EX31_RS20070 0.25 +0.2 644,868 + EX31_RS20070 0.25 +1.1 644,869 - EX31_RS20070 0.25 -0.1 644,869 - EX31_RS20070 0.25 -2.2 644,869 - EX31_RS20070 0.25 +0.9 644,869 - EX31_RS20070 0.25 +0.4 644,899 + EX31_RS20070 0.33 -0.3 644,902 + EX31_RS20070 0.33 -1.2 644,902 + EX31_RS20070 0.33 -0.1 644,903 - EX31_RS20070 0.34 -1.5 644,925 + EX31_RS20070 0.39 -1.4 644,962 + EX31_RS20070 0.48 +0.2 644,963 - EX31_RS20070 0.48 +0.0 644,963 - EX31_RS20070 0.48 -1.2 644,963 - EX31_RS20070 0.48 +1.4 644,986 + EX31_RS20070 0.54 +1.0 644,990 - EX31_RS20070 0.55 -1.0 645,100 + EX31_RS20070 0.81 -0.3 645,100 + EX31_RS20070 0.81 -0.4 645,100 + EX31_RS20070 0.81 +1.0 645,100 + EX31_RS20070 0.81 -1.6 645,101 - EX31_RS20070 0.81 -0.5 645,101 - EX31_RS20070 0.81 -1.1 645,101 - EX31_RS20070 0.81 +0.9 645,176 - +1.2 645,187 + +0.8 645,188 - +0.3 645,225 + +0.6 645,225 + +0.0 645,228 + +1.2 645,228 + +1.3 645,228 + -0.4 645,251 - +0.0 645,260 + +1.0 645,260 + +1.3 645,260 + +0.2 645,260 + -1.1 645,260 + +0.7 645,260 + +0.5 645,261 - +0.8 645,261 - -1.6 645,261 - +1.1 645,261 - +1.5 645,261 - +1.0 645,275 - +0.3 645,282 - +0.4 645,332 + -0.4 645,335 + -0.4 645,335 + +0.0 645,421 - EX31_RS20075 0.14 -1.9 645,454 - EX31_RS20075 0.17 +0.6 645,454 - EX31_RS20075 0.17 +0.4 645,454 - EX31_RS20075 0.17 +2.5 645,454 - EX31_RS20075 0.17 +0.5 645,473 + EX31_RS20075 0.18 -0.3 645,474 - EX31_RS20075 0.18 +1.3 645,474 - EX31_RS20075 0.18 +0.0 645,497 + EX31_RS20075 0.20 +0.8 645,498 - EX31_RS20075 0.20 -0.3 645,498 - EX31_RS20075 0.20 -1.0 645,520 + EX31_RS20075 0.22 +0.6 645,520 + EX31_RS20075 0.22 -0.1 645,521 - EX31_RS20075 0.22 +0.4 645,546 + EX31_RS20075 0.24 -0.1 645,547 - EX31_RS20075 0.24 -1.0 645,575 + EX31_RS20075 0.27 +0.2 645,575 + EX31_RS20075 0.27 -0.3 645,575 + EX31_RS20075 0.27 +0.0 645,575 + EX31_RS20075 0.27 +0.2 645,576 - EX31_RS20075 0.27 +0.8 645,576 - EX31_RS20075 0.27 +1.3 645,576 - EX31_RS20075 0.27 +1.2 645,576 - EX31_RS20075 0.27 +0.0 645,576 - EX31_RS20075 0.27 +0.5 645,576 - EX31_RS20075 0.27 +1.3 645,576 - EX31_RS20075 0.27 -0.3 645,576 - EX31_RS20075 0.27 +1.0 645,576 - EX31_RS20075 0.27 +0.2 645,576 - EX31_RS20075 0.27 -2.6 645,582 + EX31_RS20075 0.27 -1.0 645,655 + EX31_RS20075 0.33 -0.5 645,656 - EX31_RS20075 0.33 +0.1 645,656 - EX31_RS20075 0.33 +1.0 645,787 + EX31_RS20075 0.44 +1.2 645,841 + EX31_RS20075 0.48 -0.1 645,841 + EX31_RS20075 0.48 -1.5 645,842 - EX31_RS20075 0.48 +0.4 645,842 - EX31_RS20075 0.48 -0.9 645,857 + EX31_RS20075 0.49 -0.1 645,889 + EX31_RS20075 0.52 +1.5 645,889 + EX31_RS20075 0.52 +0.2 645,889 + EX31_RS20075 0.52 +0.5 645,890 - EX31_RS20075 0.52 +0.7 645,922 - EX31_RS20075 0.55 +0.3 645,977 + EX31_RS20075 0.59 +0.9 645,977 + EX31_RS20075 0.59 +0.8 645,977 + EX31_RS20075 0.59 -0.1 645,978 - EX31_RS20075 0.59 +0.9 645,978 - EX31_RS20075 0.59 +0.0 645,978 - EX31_RS20075 0.59 -0.4 645,978 - EX31_RS20075 0.59 +0.4 645,978 - EX31_RS20075 0.59 +1.4 645,978 - EX31_RS20075 0.59 +0.6 645,978 - EX31_RS20075 0.59 +0.0 645,978 - EX31_RS20075 0.59 +1.2 645,979 + EX31_RS20075 0.59 +1.3 645,979 + EX31_RS20075 0.59 +0.4 645,980 - EX31_RS20075 0.59 +1.2 645,980 - EX31_RS20075 0.59 -0.6 645,980 - EX31_RS20075 0.59 -0.2 645,980 - EX31_RS20075 0.59 +1.0 645,980 - EX31_RS20075 0.59 +0.2 646,058 + EX31_RS20075 0.66 +0.4 646,061 + EX31_RS20075 0.66 +0.3 646,062 - EX31_RS20075 0.66 -1.6 646,079 + EX31_RS20075 0.67 +0.2 646,083 + EX31_RS20075 0.68 -1.7 646,083 + EX31_RS20075 0.68 +0.1 646,083 + EX31_RS20075 0.68 +1.1 646,083 + EX31_RS20075 0.68 -2.1 646,083 + EX31_RS20075 0.68 +0.1 646,084 - EX31_RS20075 0.68 +0.2 646,084 - EX31_RS20075 0.68 +1.4 646,095 + EX31_RS20075 0.69 -0.1 646,096 - EX31_RS20075 0.69 -0.5 646,143 - EX31_RS20075 0.73 -0.0
Or see this region's nucleotide sequence