Experiment: L-Serine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS19900 and EX31_RS19905 are separated by 147 nucleotides EX31_RS19905 and EX31_RS19910 overlap by 4 nucleotides EX31_RS19910 and EX31_RS19915 are separated by 10 nucleotides
EX31_RS19900: EX31_RS19900 - formate hydrogenlyase regulator HycA, at 609,531 to 609,998
_RS19900
EX31_RS19905: EX31_RS19905 - 4Fe-4S dicluster domain-containing protein, at 610,146 to 610,775
_RS19905
EX31_RS19910: EX31_RS19910 - NADH dehydrogenase, at 610,772 to 612,676
_RS19910
EX31_RS19915: EX31_RS19915 - respiratory chain complex I subunit 1 family protein, at 612,687 to 613,640
_RS19915
Position (kb) 610
611
612
613 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 609.867 kb on - strand, within EX31_RS19900 at 609.869 kb on + strand, within EX31_RS19900 at 609.875 kb on + strand, within EX31_RS19900 at 609.875 kb on + strand, within EX31_RS19900 at 609.875 kb on + strand, within EX31_RS19900 at 609.875 kb on + strand, within EX31_RS19900 at 609.876 kb on - strand, within EX31_RS19900 at 609.897 kb on + strand, within EX31_RS19900 at 609.898 kb on - strand, within EX31_RS19900 at 609.898 kb on - strand, within EX31_RS19900 at 609.898 kb on - strand, within EX31_RS19900 at 609.898 kb on - strand, within EX31_RS19900 at 609.918 kb on - strand, within EX31_RS19900 at 609.922 kb on - strand, within EX31_RS19900 at 609.922 kb on - strand, within EX31_RS19900 at 609.922 kb on - strand, within EX31_RS19900 at 609.951 kb on - strand, within EX31_RS19900 at 610.001 kb on + strand at 610.001 kb on + strand at 610.001 kb on + strand at 610.002 kb on - strand at 610.002 kb on - strand at 610.002 kb on - strand at 610.013 kb on + strand at 610.014 kb on - strand at 610.014 kb on - strand at 610.014 kb on - strand at 610.014 kb on - strand at 610.031 kb on + strand at 610.072 kb on - strand at 610.082 kb on - strand at 610.082 kb on - strand at 610.082 kb on - strand at 610.101 kb on + strand at 610.101 kb on + strand at 610.102 kb on - strand at 610.102 kb on - strand at 610.102 kb on - strand at 610.102 kb on - strand at 610.131 kb on + strand at 610.132 kb on - strand at 610.132 kb on - strand at 610.132 kb on - strand at 610.173 kb on + strand at 610.173 kb on + strand at 610.173 kb on + strand at 610.173 kb on + strand at 610.173 kb on + strand at 610.174 kb on - strand at 610.180 kb on + strand at 610.200 kb on - strand at 610.200 kb on - strand at 610.211 kb on + strand, within EX31_RS19905 at 610.211 kb on + strand, within EX31_RS19905 at 610.211 kb on + strand, within EX31_RS19905 at 610.212 kb on - strand, within EX31_RS19905 at 610.255 kb on + strand, within EX31_RS19905 at 610.364 kb on + strand, within EX31_RS19905 at 610.364 kb on + strand, within EX31_RS19905 at 610.496 kb on + strand, within EX31_RS19905 at 610.496 kb on + strand, within EX31_RS19905 at 610.497 kb on - strand, within EX31_RS19905 at 610.497 kb on - strand, within EX31_RS19905 at 610.497 kb on - strand, within EX31_RS19905 at 610.597 kb on + strand, within EX31_RS19905 at 610.720 kb on + strand at 610.720 kb on + strand at 610.721 kb on - strand at 610.721 kb on - strand at 610.746 kb on - strand at 610.782 kb on + strand at 610.782 kb on + strand at 610.782 kb on + strand at 610.783 kb on - strand at 610.786 kb on - strand at 610.846 kb on + strand at 610.846 kb on + strand at 610.847 kb on - strand at 610.858 kb on - strand at 610.878 kb on + strand at 610.879 kb on - strand at 610.879 kb on - strand at 610.879 kb on - strand at 610.930 kb on + strand at 610.930 kb on + strand at 610.930 kb on + strand at 610.931 kb on - strand at 610.931 kb on - strand at 610.950 kb on + strand at 610.951 kb on - strand at 610.975 kb on + strand, within EX31_RS19910 at 610.975 kb on + strand, within EX31_RS19910 at 610.975 kb on + strand, within EX31_RS19910 at 610.975 kb on + strand, within EX31_RS19910 at 610.975 kb on + strand, within EX31_RS19910 at 610.976 kb on - strand, within EX31_RS19910 at 611.070 kb on - strand, within EX31_RS19910 at 611.070 kb on - strand, within EX31_RS19910 at 611.070 kb on - strand, within EX31_RS19910 at 611.128 kb on - strand, within EX31_RS19910 at 611.222 kb on + strand, within EX31_RS19910 at 611.222 kb on + strand, within EX31_RS19910 at 611.223 kb on - strand, within EX31_RS19910 at 611.235 kb on + strand, within EX31_RS19910 at 611.235 kb on + strand, within EX31_RS19910 at 611.235 kb on + strand, within EX31_RS19910 at 611.235 kb on + strand, within EX31_RS19910 at 611.235 kb on + strand, within EX31_RS19910 at 611.235 kb on + strand, within EX31_RS19910 at 611.236 kb on - strand, within EX31_RS19910 at 611.236 kb on - strand, within EX31_RS19910 at 611.236 kb on - strand, within EX31_RS19910 at 611.236 kb on - strand, within EX31_RS19910 at 611.236 kb on - strand, within EX31_RS19910 at 611.236 kb on - strand, within EX31_RS19910 at 611.383 kb on + strand, within EX31_RS19910 at 611.395 kb on + strand, within EX31_RS19910 at 611.396 kb on - strand, within EX31_RS19910 at 611.438 kb on + strand, within EX31_RS19910 at 611.450 kb on - strand, within EX31_RS19910 at 611.553 kb on + strand, within EX31_RS19910 at 611.553 kb on + strand, within EX31_RS19910 at 611.553 kb on + strand, within EX31_RS19910 at 611.554 kb on - strand, within EX31_RS19910 at 611.554 kb on - strand, within EX31_RS19910 at 611.554 kb on - strand, within EX31_RS19910 at 611.554 kb on - strand, within EX31_RS19910 at 611.607 kb on + strand, within EX31_RS19910 at 611.607 kb on + strand, within EX31_RS19910 at 611.608 kb on - strand, within EX31_RS19910 at 611.687 kb on + strand, within EX31_RS19910 at 611.825 kb on - strand, within EX31_RS19910 at 611.875 kb on + strand, within EX31_RS19910 at 611.876 kb on - strand, within EX31_RS19910 at 611.876 kb on - strand, within EX31_RS19910 at 612.002 kb on + strand, within EX31_RS19910 at 612.003 kb on - strand, within EX31_RS19910 at 612.041 kb on - strand, within EX31_RS19910 at 612.109 kb on + strand, within EX31_RS19910 at 612.109 kb on + strand, within EX31_RS19910 at 612.110 kb on - strand, within EX31_RS19910 at 612.110 kb on - strand, within EX31_RS19910 at 612.110 kb on - strand, within EX31_RS19910 at 612.207 kb on + strand, within EX31_RS19910 at 612.207 kb on + strand, within EX31_RS19910 at 612.208 kb on - strand, within EX31_RS19910 at 612.208 kb on - strand, within EX31_RS19910 at 612.208 kb on - strand, within EX31_RS19910 at 612.418 kb on - strand, within EX31_RS19910 at 612.686 kb on + strand at 612.686 kb on + strand at 612.790 kb on + strand, within EX31_RS19915 at 612.791 kb on - strand, within EX31_RS19915 at 612.841 kb on - strand, within EX31_RS19915 at 612.841 kb on - strand, within EX31_RS19915 at 612.848 kb on + strand, within EX31_RS19915 at 612.849 kb on - strand, within EX31_RS19915 at 612.849 kb on - strand, within EX31_RS19915 at 612.919 kb on - strand, within EX31_RS19915 at 612.938 kb on - strand, within EX31_RS19915 at 613.017 kb on + strand, within EX31_RS19915 at 613.017 kb on + strand, within EX31_RS19915 at 613.017 kb on + strand, within EX31_RS19915 at 613.018 kb on - strand, within EX31_RS19915 at 613.018 kb on - strand, within EX31_RS19915 at 613.018 kb on - strand, within EX31_RS19915 at 613.018 kb on - strand, within EX31_RS19915 at 613.018 kb on - strand, within EX31_RS19915 at 613.031 kb on - strand, within EX31_RS19915 at 613.231 kb on + strand, within EX31_RS19915 at 613.272 kb on + strand, within EX31_RS19915 at 613.272 kb on + strand, within EX31_RS19915 at 613.272 kb on + strand, within EX31_RS19915 at 613.272 kb on + strand, within EX31_RS19915 at 613.272 kb on + strand, within EX31_RS19915 at 613.272 kb on + strand, within EX31_RS19915 at 613.272 kb on + strand, within EX31_RS19915 at 613.272 kb on + strand, within EX31_RS19915 at 613.272 kb on + strand, within EX31_RS19915 at 613.272 kb on + strand, within EX31_RS19915 at 613.272 kb on + strand, within EX31_RS19915 at 613.272 kb on + strand, within EX31_RS19915 at 613.272 kb on + strand, within EX31_RS19915 at 613.273 kb on - strand, within EX31_RS19915 at 613.273 kb on - strand, within EX31_RS19915 at 613.273 kb on - strand, within EX31_RS19915 at 613.273 kb on - strand, within EX31_RS19915 at 613.273 kb on - strand, within EX31_RS19915 at 613.273 kb on - strand, within EX31_RS19915 at 613.273 kb on - strand, within EX31_RS19915 at 613.273 kb on - strand, within EX31_RS19915 at 613.273 kb on - strand, within EX31_RS19915 at 613.273 kb on - strand, within EX31_RS19915 at 613.273 kb on - strand, within EX31_RS19915 at 613.324 kb on + strand, within EX31_RS19915 at 613.324 kb on + strand, within EX31_RS19915 at 613.324 kb on + strand, within EX31_RS19915 at 613.414 kb on + strand, within EX31_RS19915 at 613.414 kb on + strand, within EX31_RS19915 at 613.415 kb on - strand, within EX31_RS19915 at 613.415 kb on - strand, within EX31_RS19915 at 613.415 kb on - strand, within EX31_RS19915 at 613.517 kb on - strand, within EX31_RS19915 at 613.565 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; no shaking remove 609,867 - EX31_RS19900 0.72 -0.5 609,869 + EX31_RS19900 0.72 -2.7 609,875 + EX31_RS19900 0.74 -0.2 609,875 + EX31_RS19900 0.74 +1.3 609,875 + EX31_RS19900 0.74 +0.2 609,875 + EX31_RS19900 0.74 +1.5 609,876 - EX31_RS19900 0.74 +1.3 609,897 + EX31_RS19900 0.78 +0.2 609,898 - EX31_RS19900 0.78 -0.2 609,898 - EX31_RS19900 0.78 -1.9 609,898 - EX31_RS19900 0.78 -1.6 609,898 - EX31_RS19900 0.78 -0.4 609,918 - EX31_RS19900 0.83 +0.4 609,922 - EX31_RS19900 0.84 +0.6 609,922 - EX31_RS19900 0.84 +0.5 609,922 - EX31_RS19900 0.84 -1.2 609,951 - EX31_RS19900 0.90 -1.7 610,001 + -0.2 610,001 + -0.2 610,001 + +0.6 610,002 - +0.1 610,002 - +0.5 610,002 - -0.6 610,013 + +0.4 610,014 - +0.3 610,014 - +0.4 610,014 - +1.6 610,014 - +0.2 610,031 + +0.8 610,072 - +0.4 610,082 - -1.3 610,082 - +0.1 610,082 - +0.9 610,101 + -0.7 610,101 + +0.6 610,102 - +0.5 610,102 - +0.9 610,102 - -1.0 610,102 - -2.2 610,131 + +1.7 610,132 - +2.1 610,132 - -0.1 610,132 - +0.6 610,173 + +0.8 610,173 + -0.3 610,173 + +0.0 610,173 + -1.0 610,173 + +1.6 610,174 - +1.5 610,180 + -0.4 610,200 - -1.0 610,200 - -0.2 610,211 + EX31_RS19905 0.10 -0.4 610,211 + EX31_RS19905 0.10 +0.7 610,211 + EX31_RS19905 0.10 -0.4 610,212 - EX31_RS19905 0.10 -0.7 610,255 + EX31_RS19905 0.17 -0.7 610,364 + EX31_RS19905 0.35 -0.7 610,364 + EX31_RS19905 0.35 +1.6 610,496 + EX31_RS19905 0.56 -0.7 610,496 + EX31_RS19905 0.56 +1.5 610,497 - EX31_RS19905 0.56 +1.4 610,497 - EX31_RS19905 0.56 +0.8 610,497 - EX31_RS19905 0.56 -2.2 610,597 + EX31_RS19905 0.72 -0.7 610,720 + +2.1 610,720 + -0.1 610,721 - +0.1 610,721 - +1.1 610,746 - -1.8 610,782 + +0.3 610,782 + -1.6 610,782 + -0.4 610,783 - +0.8 610,786 - +0.7 610,846 + +1.0 610,846 + -0.2 610,847 - +0.4 610,858 - -0.6 610,878 + -0.0 610,879 - +1.4 610,879 - -0.5 610,879 - -0.8 610,930 + +0.4 610,930 + -0.7 610,930 + +1.2 610,931 - -0.3 610,931 - +0.3 610,950 + +0.7 610,951 - +0.9 610,975 + EX31_RS19910 0.11 +0.8 610,975 + EX31_RS19910 0.11 +0.4 610,975 + EX31_RS19910 0.11 +0.3 610,975 + EX31_RS19910 0.11 +0.5 610,975 + EX31_RS19910 0.11 -0.1 610,976 - EX31_RS19910 0.11 -0.2 611,070 - EX31_RS19910 0.16 +1.0 611,070 - EX31_RS19910 0.16 -0.1 611,070 - EX31_RS19910 0.16 -1.4 611,128 - EX31_RS19910 0.19 +1.3 611,222 + EX31_RS19910 0.24 -0.3 611,222 + EX31_RS19910 0.24 +0.1 611,223 - EX31_RS19910 0.24 -0.8 611,235 + EX31_RS19910 0.24 -0.4 611,235 + EX31_RS19910 0.24 +0.0 611,235 + EX31_RS19910 0.24 +0.2 611,235 + EX31_RS19910 0.24 -0.0 611,235 + EX31_RS19910 0.24 -1.9 611,235 + EX31_RS19910 0.24 +1.5 611,236 - EX31_RS19910 0.24 +0.6 611,236 - EX31_RS19910 0.24 +1.0 611,236 - EX31_RS19910 0.24 +0.9 611,236 - EX31_RS19910 0.24 -0.7 611,236 - EX31_RS19910 0.24 -1.2 611,236 - EX31_RS19910 0.24 -1.3 611,383 + EX31_RS19910 0.32 +0.6 611,395 + EX31_RS19910 0.33 +1.3 611,396 - EX31_RS19910 0.33 +1.1 611,438 + EX31_RS19910 0.35 -1.0 611,450 - EX31_RS19910 0.36 +0.3 611,553 + EX31_RS19910 0.41 +0.8 611,553 + EX31_RS19910 0.41 -0.7 611,553 + EX31_RS19910 0.41 -0.2 611,554 - EX31_RS19910 0.41 -2.2 611,554 - EX31_RS19910 0.41 -0.5 611,554 - EX31_RS19910 0.41 -0.2 611,554 - EX31_RS19910 0.41 +1.1 611,607 + EX31_RS19910 0.44 -2.1 611,607 + EX31_RS19910 0.44 -0.1 611,608 - EX31_RS19910 0.44 +1.1 611,687 + EX31_RS19910 0.48 -0.7 611,825 - EX31_RS19910 0.55 -1.4 611,875 + EX31_RS19910 0.58 -0.0 611,876 - EX31_RS19910 0.58 -1.3 611,876 - EX31_RS19910 0.58 -0.4 612,002 + EX31_RS19910 0.65 -0.4 612,003 - EX31_RS19910 0.65 +1.3 612,041 - EX31_RS19910 0.67 +1.1 612,109 + EX31_RS19910 0.70 +0.9 612,109 + EX31_RS19910 0.70 +0.2 612,110 - EX31_RS19910 0.70 +0.5 612,110 - EX31_RS19910 0.70 +0.2 612,110 - EX31_RS19910 0.70 +0.5 612,207 + EX31_RS19910 0.75 +2.0 612,207 + EX31_RS19910 0.75 -0.2 612,208 - EX31_RS19910 0.75 -0.6 612,208 - EX31_RS19910 0.75 -0.4 612,208 - EX31_RS19910 0.75 -1.9 612,418 - EX31_RS19910 0.86 -0.1 612,686 + -1.1 612,686 + +2.7 612,790 + EX31_RS19915 0.11 -1.3 612,791 - EX31_RS19915 0.11 +0.2 612,841 - EX31_RS19915 0.16 -1.5 612,841 - EX31_RS19915 0.16 -0.8 612,848 + EX31_RS19915 0.17 -0.1 612,849 - EX31_RS19915 0.17 +0.3 612,849 - EX31_RS19915 0.17 +0.6 612,919 - EX31_RS19915 0.24 -2.4 612,938 - EX31_RS19915 0.26 -0.4 613,017 + EX31_RS19915 0.35 -0.2 613,017 + EX31_RS19915 0.35 -1.0 613,017 + EX31_RS19915 0.35 +0.0 613,018 - EX31_RS19915 0.35 +0.9 613,018 - EX31_RS19915 0.35 +0.6 613,018 - EX31_RS19915 0.35 +0.1 613,018 - EX31_RS19915 0.35 +1.4 613,018 - EX31_RS19915 0.35 +0.6 613,031 - EX31_RS19915 0.36 +0.2 613,231 + EX31_RS19915 0.57 +0.8 613,272 + EX31_RS19915 0.61 -1.6 613,272 + EX31_RS19915 0.61 +0.0 613,272 + EX31_RS19915 0.61 -0.5 613,272 + EX31_RS19915 0.61 +0.3 613,272 + EX31_RS19915 0.61 -0.1 613,272 + EX31_RS19915 0.61 +0.6 613,272 + EX31_RS19915 0.61 -0.4 613,272 + EX31_RS19915 0.61 -3.0 613,272 + EX31_RS19915 0.61 -2.6 613,272 + EX31_RS19915 0.61 -1.3 613,272 + EX31_RS19915 0.61 +1.1 613,272 + EX31_RS19915 0.61 -0.2 613,272 + EX31_RS19915 0.61 +1.3 613,273 - EX31_RS19915 0.61 +2.6 613,273 - EX31_RS19915 0.61 +1.4 613,273 - EX31_RS19915 0.61 -0.1 613,273 - EX31_RS19915 0.61 +0.5 613,273 - EX31_RS19915 0.61 +0.3 613,273 - EX31_RS19915 0.61 +0.5 613,273 - EX31_RS19915 0.61 +0.1 613,273 - EX31_RS19915 0.61 +0.6 613,273 - EX31_RS19915 0.61 +0.1 613,273 - EX31_RS19915 0.61 +1.0 613,273 - EX31_RS19915 0.61 -0.2 613,324 + EX31_RS19915 0.67 -0.7 613,324 + EX31_RS19915 0.67 -0.1 613,324 + EX31_RS19915 0.67 +0.6 613,414 + EX31_RS19915 0.76 -0.9 613,414 + EX31_RS19915 0.76 -0.1 613,415 - EX31_RS19915 0.76 -1.7 613,415 - EX31_RS19915 0.76 +1.1 613,415 - EX31_RS19915 0.76 -1.7 613,517 - EX31_RS19915 0.87 +0.3 613,565 + -0.2
Or see this region's nucleotide sequence