Experiment: L-Serine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS18915 and EX31_RS18920 are separated by 622 nucleotides EX31_RS18920 and EX31_RS18925 overlap by 4 nucleotides EX31_RS18925 and EX31_RS18930 are separated by 30 nucleotides
EX31_RS18915: EX31_RS18915 - DNA starvation/stationary phase protection protein Dps, at 368,662 to 369,165
_RS18915
EX31_RS18920: EX31_RS18920 - ABC transporter permease, at 369,788 to 370,564
_RS18920
EX31_RS18925: EX31_RS18925 - ABC transporter ATP-binding protein, at 370,561 to 371,226
_RS18925
EX31_RS18930: EX31_RS18930 - capsular biosynthesis protein, at 371,257 to 372,249
_RS18930
Position (kb) 369
370
371 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 368.839 kb on - strand, within EX31_RS18915 at 368.921 kb on + strand, within EX31_RS18915 at 368.921 kb on + strand, within EX31_RS18915 at 368.921 kb on + strand, within EX31_RS18915 at 368.922 kb on - strand, within EX31_RS18915 at 368.922 kb on - strand, within EX31_RS18915 at 368.922 kb on - strand, within EX31_RS18915 at 368.962 kb on - strand, within EX31_RS18915 at 369.034 kb on + strand, within EX31_RS18915 at 369.035 kb on - strand, within EX31_RS18915 at 369.078 kb on - strand, within EX31_RS18915 at 369.099 kb on + strand, within EX31_RS18915 at 369.156 kb on + strand at 369.156 kb on + strand at 369.157 kb on - strand at 369.164 kb on - strand at 369.190 kb on + strand at 369.190 kb on + strand at 369.191 kb on - strand at 369.211 kb on + strand at 369.266 kb on + strand at 369.267 kb on - strand at 369.267 kb on - strand at 369.267 kb on - strand at 369.296 kb on + strand at 369.297 kb on - strand at 369.297 kb on - strand at 369.322 kb on - strand at 369.322 kb on - strand at 369.327 kb on + strand at 369.328 kb on - strand at 369.328 kb on - strand at 369.352 kb on + strand at 369.353 kb on - strand at 369.422 kb on - strand at 369.488 kb on - strand at 369.510 kb on + strand at 369.510 kb on + strand at 369.587 kb on + strand at 369.653 kb on + strand at 369.653 kb on + strand at 369.781 kb on + strand at 369.796 kb on + strand at 369.797 kb on - strand at 369.804 kb on + strand at 369.804 kb on + strand at 369.805 kb on - strand at 369.810 kb on + strand at 369.810 kb on + strand at 369.865 kb on + strand at 369.869 kb on + strand, within EX31_RS18920 at 369.869 kb on + strand, within EX31_RS18920 at 369.870 kb on - strand, within EX31_RS18920 at 369.881 kb on + strand, within EX31_RS18920 at 369.881 kb on + strand, within EX31_RS18920 at 370.024 kb on + strand, within EX31_RS18920 at 370.026 kb on + strand, within EX31_RS18920 at 370.026 kb on + strand, within EX31_RS18920 at 370.026 kb on + strand, within EX31_RS18920 at 370.108 kb on - strand, within EX31_RS18920 at 370.218 kb on + strand, within EX31_RS18920 at 370.233 kb on + strand, within EX31_RS18920 at 370.350 kb on + strand, within EX31_RS18920 at 370.395 kb on + strand, within EX31_RS18920 at 370.419 kb on + strand, within EX31_RS18920 at 370.477 kb on - strand, within EX31_RS18920 at 370.509 kb on + strand at 370.527 kb on + strand at 370.531 kb on + strand at 370.531 kb on + strand at 370.593 kb on + strand at 370.615 kb on + strand at 370.654 kb on - strand, within EX31_RS18925 at 370.755 kb on + strand, within EX31_RS18925 at 370.755 kb on + strand, within EX31_RS18925 at 370.755 kb on + strand, within EX31_RS18925 at 370.755 kb on + strand, within EX31_RS18925 at 370.755 kb on + strand, within EX31_RS18925 at 370.761 kb on + strand, within EX31_RS18925 at 370.761 kb on + strand, within EX31_RS18925 at 370.761 kb on + strand, within EX31_RS18925 at 370.761 kb on + strand, within EX31_RS18925 at 370.762 kb on - strand, within EX31_RS18925 at 370.997 kb on - strand, within EX31_RS18925 at 371.000 kb on + strand, within EX31_RS18925 at 371.061 kb on + strand, within EX31_RS18925 at 371.195 kb on + strand at 371.241 kb on - strand at 371.483 kb on + strand, within EX31_RS18930 at 371.544 kb on + strand, within EX31_RS18930
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; no shaking remove 368,839 - EX31_RS18915 0.35 +1.3 368,921 + EX31_RS18915 0.51 -0.5 368,921 + EX31_RS18915 0.51 +0.5 368,921 + EX31_RS18915 0.51 +0.2 368,922 - EX31_RS18915 0.52 +0.0 368,922 - EX31_RS18915 0.52 -0.5 368,922 - EX31_RS18915 0.52 +1.7 368,962 - EX31_RS18915 0.60 -1.3 369,034 + EX31_RS18915 0.74 -0.7 369,035 - EX31_RS18915 0.74 -2.1 369,078 - EX31_RS18915 0.83 -2.0 369,099 + EX31_RS18915 0.87 -0.8 369,156 + +1.1 369,156 + -1.0 369,157 - +0.1 369,164 - -1.0 369,190 + -0.5 369,190 + +0.3 369,191 - +2.0 369,211 + -0.0 369,266 + +1.7 369,267 - -0.8 369,267 - +0.6 369,267 - -1.4 369,296 + +0.3 369,297 - +0.5 369,297 - +0.9 369,322 - -0.5 369,322 - +0.2 369,327 + +0.8 369,328 - -0.7 369,328 - -0.5 369,352 + +1.8 369,353 - -0.3 369,422 - +0.3 369,488 - -1.9 369,510 + -1.3 369,510 + +1.2 369,587 + -1.5 369,653 + -1.3 369,653 + -1.5 369,781 + -0.8 369,796 + -0.2 369,797 - +0.5 369,804 + -0.6 369,804 + +0.1 369,805 - -1.4 369,810 + -1.0 369,810 + +1.4 369,865 + -1.0 369,869 + EX31_RS18920 0.10 -1.1 369,869 + EX31_RS18920 0.10 +2.5 369,870 - EX31_RS18920 0.11 +0.7 369,881 + EX31_RS18920 0.12 -2.1 369,881 + EX31_RS18920 0.12 -1.0 370,024 + EX31_RS18920 0.30 -0.4 370,026 + EX31_RS18920 0.31 +0.7 370,026 + EX31_RS18920 0.31 +0.5 370,026 + EX31_RS18920 0.31 +1.6 370,108 - EX31_RS18920 0.41 -0.6 370,218 + EX31_RS18920 0.55 +1.3 370,233 + EX31_RS18920 0.57 +0.2 370,350 + EX31_RS18920 0.72 +0.7 370,395 + EX31_RS18920 0.78 +1.2 370,419 + EX31_RS18920 0.81 +1.5 370,477 - EX31_RS18920 0.89 -0.5 370,509 + -2.1 370,527 + +0.3 370,531 + +0.7 370,531 + -0.7 370,593 + -1.7 370,615 + -3.4 370,654 - EX31_RS18925 0.14 -0.5 370,755 + EX31_RS18925 0.29 +0.6 370,755 + EX31_RS18925 0.29 -0.8 370,755 + EX31_RS18925 0.29 +1.0 370,755 + EX31_RS18925 0.29 -0.8 370,755 + EX31_RS18925 0.29 -1.1 370,761 + EX31_RS18925 0.30 -0.5 370,761 + EX31_RS18925 0.30 +1.6 370,761 + EX31_RS18925 0.30 -1.0 370,761 + EX31_RS18925 0.30 -0.5 370,762 - EX31_RS18925 0.30 +2.0 370,997 - EX31_RS18925 0.65 +0.0 371,000 + EX31_RS18925 0.66 +0.8 371,061 + EX31_RS18925 0.75 +0.3 371,195 + +1.4 371,241 - -0.3 371,483 + EX31_RS18930 0.23 +1.6 371,544 + EX31_RS18930 0.29 +1.8
Or see this region's nucleotide sequence