Experiment: L-Serine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS11600 and EX31_RS11605 are separated by 178 nucleotides EX31_RS11605 and EX31_RS11610 are separated by 103 nucleotides EX31_RS11610 and EX31_RS11615 are separated by 92 nucleotides EX31_RS11615 and EX31_RS11620 are separated by 20 nucleotides
EX31_RS11600: EX31_RS11600 - CocE/NonD family hydrolase, at 260,361 to 262,388
_RS11600
EX31_RS11605: EX31_RS11605 - helix-turn-helix transcriptional regulator, at 262,567 to 263,013
_RS11605
EX31_RS11610: EX31_RS11610 - SDR family oxidoreductase, at 263,117 to 263,911
_RS11610
EX31_RS11615: EX31_RS11615 - fumarylacetoacetate hydrolase family protein, at 264,004 to 264,693
_RS11615
EX31_RS11620: EX31_RS11620 - hypothetical protein, at 264,714 to 265,520
_RS11620
Position (kb) 263
264 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 262.190 kb on + strand at 262.363 kb on - strand at 262.406 kb on - strand at 262.507 kb on - strand at 262.582 kb on - strand at 262.592 kb on - strand at 262.592 kb on - strand at 262.592 kb on - strand at 262.592 kb on - strand at 262.641 kb on + strand, within EX31_RS11605 at 262.670 kb on - strand, within EX31_RS11605 at 262.670 kb on - strand, within EX31_RS11605 at 262.707 kb on - strand, within EX31_RS11605 at 262.707 kb on - strand, within EX31_RS11605 at 262.707 kb on - strand, within EX31_RS11605 at 262.775 kb on + strand, within EX31_RS11605 at 262.776 kb on - strand, within EX31_RS11605 at 262.776 kb on - strand, within EX31_RS11605 at 262.776 kb on - strand, within EX31_RS11605 at 262.819 kb on - strand, within EX31_RS11605 at 262.914 kb on - strand, within EX31_RS11605 at 262.914 kb on - strand, within EX31_RS11605 at 262.914 kb on - strand, within EX31_RS11605 at 262.914 kb on - strand, within EX31_RS11605 at 263.113 kb on + strand at 263.114 kb on - strand at 263.114 kb on - strand at 263.114 kb on - strand at 263.114 kb on - strand at 263.114 kb on - strand at 263.114 kb on - strand at 263.114 kb on - strand at 263.114 kb on - strand at 263.114 kb on - strand at 263.116 kb on + strand at 263.116 kb on + strand at 263.116 kb on + strand at 263.117 kb on - strand at 263.125 kb on + strand at 263.125 kb on + strand at 263.186 kb on + strand at 263.187 kb on - strand at 263.187 kb on - strand at 263.187 kb on - strand at 263.187 kb on - strand at 263.187 kb on - strand at 263.220 kb on + strand, within EX31_RS11610 at 263.220 kb on + strand, within EX31_RS11610 at 263.455 kb on + strand, within EX31_RS11610 at 263.519 kb on - strand, within EX31_RS11610 at 263.519 kb on - strand, within EX31_RS11610 at 263.536 kb on + strand, within EX31_RS11610 at 263.537 kb on - strand, within EX31_RS11610 at 263.537 kb on - strand, within EX31_RS11610 at 263.583 kb on + strand, within EX31_RS11610 at 263.584 kb on - strand, within EX31_RS11610 at 263.585 kb on + strand, within EX31_RS11610 at 263.585 kb on + strand, within EX31_RS11610 at 263.657 kb on + strand, within EX31_RS11610 at 263.657 kb on + strand, within EX31_RS11610 at 263.658 kb on - strand, within EX31_RS11610 at 263.900 kb on + strand at 263.936 kb on - strand at 263.936 kb on - strand at 263.970 kb on + strand at 263.971 kb on - strand at 263.971 kb on - strand at 263.971 kb on - strand at 263.975 kb on + strand at 263.976 kb on - strand at 264.013 kb on + strand at 264.013 kb on + strand at 264.017 kb on + strand at 264.017 kb on + strand at 264.018 kb on - strand at 264.018 kb on - strand at 264.018 kb on - strand at 264.018 kb on - strand at 264.089 kb on - strand, within EX31_RS11615 at 264.106 kb on + strand, within EX31_RS11615 at 264.107 kb on - strand, within EX31_RS11615 at 264.238 kb on + strand, within EX31_RS11615 at 264.282 kb on + strand, within EX31_RS11615 at 264.282 kb on + strand, within EX31_RS11615 at 264.319 kb on + strand, within EX31_RS11615 at 264.333 kb on - strand, within EX31_RS11615 at 264.333 kb on - strand, within EX31_RS11615 at 264.339 kb on - strand, within EX31_RS11615 at 264.537 kb on - strand, within EX31_RS11615 at 264.553 kb on + strand, within EX31_RS11615 at 264.553 kb on + strand, within EX31_RS11615 at 264.554 kb on - strand, within EX31_RS11615 at 264.554 kb on - strand, within EX31_RS11615 at 264.595 kb on + strand, within EX31_RS11615 at 264.595 kb on + strand, within EX31_RS11615 at 264.596 kb on - strand, within EX31_RS11615 at 264.596 kb on - strand, within EX31_RS11615 at 264.596 kb on - strand, within EX31_RS11615 at 264.691 kb on + strand at 264.767 kb on + strand at 264.767 kb on + strand at 264.831 kb on + strand, within EX31_RS11620 at 264.832 kb on - strand, within EX31_RS11620 at 264.842 kb on + strand, within EX31_RS11620 at 264.843 kb on - strand, within EX31_RS11620 at 264.899 kb on - strand, within EX31_RS11620 at 264.902 kb on - strand, within EX31_RS11620 at 264.910 kb on + strand, within EX31_RS11620 at 264.911 kb on - strand, within EX31_RS11620 at 264.911 kb on - strand, within EX31_RS11620
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; no shaking remove 262,190 + +3.0 262,363 - +0.2 262,406 - +1.1 262,507 - +0.3 262,582 - -0.2 262,592 - +1.2 262,592 - -0.9 262,592 - -1.0 262,592 - +2.9 262,641 + EX31_RS11605 0.17 -0.5 262,670 - EX31_RS11605 0.23 +0.6 262,670 - EX31_RS11605 0.23 +0.3 262,707 - EX31_RS11605 0.31 -1.0 262,707 - EX31_RS11605 0.31 -0.3 262,707 - EX31_RS11605 0.31 -0.3 262,775 + EX31_RS11605 0.47 +1.1 262,776 - EX31_RS11605 0.47 +0.4 262,776 - EX31_RS11605 0.47 +0.4 262,776 - EX31_RS11605 0.47 -1.9 262,819 - EX31_RS11605 0.56 +0.4 262,914 - EX31_RS11605 0.78 -0.4 262,914 - EX31_RS11605 0.78 -2.2 262,914 - EX31_RS11605 0.78 -0.2 262,914 - EX31_RS11605 0.78 -0.9 263,113 + -0.8 263,114 - -0.3 263,114 - -0.5 263,114 - +1.5 263,114 - -1.0 263,114 - +0.2 263,114 - +0.6 263,114 - -2.3 263,114 - -2.3 263,114 - +1.3 263,116 + +0.8 263,116 + -0.4 263,116 + -0.0 263,117 - +0.7 263,125 + +0.4 263,125 + -0.8 263,186 + +0.5 263,187 - +0.2 263,187 - +0.2 263,187 - +0.7 263,187 - -1.4 263,187 - -0.2 263,220 + EX31_RS11610 0.13 -1.2 263,220 + EX31_RS11610 0.13 -0.6 263,455 + EX31_RS11610 0.43 -1.2 263,519 - EX31_RS11610 0.51 +0.5 263,519 - EX31_RS11610 0.51 -1.9 263,536 + EX31_RS11610 0.53 -0.1 263,537 - EX31_RS11610 0.53 -0.7 263,537 - EX31_RS11610 0.53 -0.1 263,583 + EX31_RS11610 0.59 -0.5 263,584 - EX31_RS11610 0.59 -0.8 263,585 + EX31_RS11610 0.59 -1.6 263,585 + EX31_RS11610 0.59 -1.0 263,657 + EX31_RS11610 0.68 -0.1 263,657 + EX31_RS11610 0.68 -0.8 263,658 - EX31_RS11610 0.68 -0.4 263,900 + -1.6 263,936 - +0.8 263,936 - +0.9 263,970 + -1.1 263,971 - -0.7 263,971 - -0.8 263,971 - +1.4 263,975 + -0.1 263,976 - -0.7 264,013 + -0.4 264,013 + +0.8 264,017 + +1.3 264,017 + +0.2 264,018 - -1.4 264,018 - -0.5 264,018 - +1.8 264,018 - +0.7 264,089 - EX31_RS11615 0.12 -0.1 264,106 + EX31_RS11615 0.15 +0.0 264,107 - EX31_RS11615 0.15 +0.1 264,238 + EX31_RS11615 0.34 +2.1 264,282 + EX31_RS11615 0.40 +1.0 264,282 + EX31_RS11615 0.40 +0.6 264,319 + EX31_RS11615 0.46 -0.0 264,333 - EX31_RS11615 0.48 +0.1 264,333 - EX31_RS11615 0.48 +1.5 264,339 - EX31_RS11615 0.49 +1.6 264,537 - EX31_RS11615 0.77 +2.2 264,553 + EX31_RS11615 0.80 -0.2 264,553 + EX31_RS11615 0.80 -1.5 264,554 - EX31_RS11615 0.80 -0.9 264,554 - EX31_RS11615 0.80 -1.3 264,595 + EX31_RS11615 0.86 -0.1 264,595 + EX31_RS11615 0.86 +0.6 264,596 - EX31_RS11615 0.86 +0.3 264,596 - EX31_RS11615 0.86 +0.4 264,596 - EX31_RS11615 0.86 +1.3 264,691 + +1.0 264,767 + -0.4 264,767 + -0.1 264,831 + EX31_RS11620 0.14 +1.4 264,832 - EX31_RS11620 0.15 -1.1 264,842 + EX31_RS11620 0.16 +1.6 264,843 - EX31_RS11620 0.16 +0.4 264,899 - EX31_RS11620 0.23 -0.2 264,902 - EX31_RS11620 0.23 +0.7 264,910 + EX31_RS11620 0.24 +1.8 264,911 - EX31_RS11620 0.24 +0.6 264,911 - EX31_RS11620 0.24 +0.2
Or see this region's nucleotide sequence