Experiment: L-Serine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS11255 and EX31_RS11260 overlap by 8 nucleotides EX31_RS11260 and EX31_RS11265 are separated by 304 nucleotides EX31_RS11265 and EX31_RS11270 are separated by 27 nucleotides
EX31_RS11255: EX31_RS11255 - CidA/LrgA family protein, at 184,012 to 184,416
_RS11255
EX31_RS11260: EX31_RS11260 - CidB/LrgB family autolysis modulator, at 184,409 to 185,104
_RS11260
EX31_RS11265: EX31_RS11265 - flagellar hook-filament junction protein FlgL, at 185,409 to 186,371
_RS11265
EX31_RS11270: EX31_RS11270 - flagellar hook-associated protein FlgK, at 186,399 to 188,051
_RS11270
Position (kb) 185
186
187 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 184.417 kb on + strand at 184.417 kb on + strand at 184.417 kb on + strand at 184.417 kb on + strand at 184.417 kb on + strand at 184.417 kb on + strand at 184.417 kb on + strand at 184.417 kb on + strand at 184.417 kb on + strand at 184.418 kb on - strand at 184.419 kb on + strand at 184.419 kb on + strand at 184.419 kb on + strand at 184.419 kb on + strand at 184.419 kb on + strand at 184.419 kb on + strand at 184.419 kb on + strand at 184.419 kb on + strand at 184.420 kb on - strand at 184.420 kb on - strand at 184.420 kb on - strand at 184.477 kb on + strand at 184.477 kb on + strand at 184.637 kb on + strand, within EX31_RS11260 at 184.637 kb on + strand, within EX31_RS11260 at 184.637 kb on + strand, within EX31_RS11260 at 184.637 kb on + strand, within EX31_RS11260 at 184.638 kb on - strand, within EX31_RS11260 at 184.638 kb on - strand, within EX31_RS11260 at 184.684 kb on + strand, within EX31_RS11260 at 184.737 kb on + strand, within EX31_RS11260 at 184.738 kb on - strand, within EX31_RS11260 at 184.773 kb on + strand, within EX31_RS11260 at 184.831 kb on + strand, within EX31_RS11260 at 184.832 kb on - strand, within EX31_RS11260 at 184.851 kb on + strand, within EX31_RS11260 at 184.852 kb on - strand, within EX31_RS11260 at 184.852 kb on - strand, within EX31_RS11260 at 184.852 kb on - strand, within EX31_RS11260 at 184.852 kb on - strand, within EX31_RS11260 at 184.852 kb on - strand, within EX31_RS11260 at 185.144 kb on + strand at 185.144 kb on + strand at 185.144 kb on + strand at 185.144 kb on + strand at 185.179 kb on + strand at 185.179 kb on + strand at 185.179 kb on + strand at 185.179 kb on + strand at 185.179 kb on + strand at 185.180 kb on - strand at 185.184 kb on + strand at 185.184 kb on + strand at 185.184 kb on + strand at 185.184 kb on + strand at 185.185 kb on - strand at 185.185 kb on - strand at 185.188 kb on + strand at 185.188 kb on + strand at 185.188 kb on + strand at 185.188 kb on + strand at 185.188 kb on + strand at 185.189 kb on - strand at 185.189 kb on - strand at 185.189 kb on - strand at 185.189 kb on - strand at 185.254 kb on + strand at 185.254 kb on + strand at 185.255 kb on - strand at 185.255 kb on - strand at 185.263 kb on + strand at 185.263 kb on + strand at 185.264 kb on - strand at 185.284 kb on + strand at 185.284 kb on + strand at 185.284 kb on + strand at 185.284 kb on + strand at 185.285 kb on - strand at 185.285 kb on - strand at 185.285 kb on - strand at 185.307 kb on + strand at 185.312 kb on - strand at 185.394 kb on - strand at 185.394 kb on - strand at 185.407 kb on + strand at 185.408 kb on - strand at 185.408 kb on - strand at 185.435 kb on + strand at 185.435 kb on + strand at 185.435 kb on + strand at 185.436 kb on - strand at 185.436 kb on - strand at 185.436 kb on - strand at 185.436 kb on - strand at 185.452 kb on + strand at 185.453 kb on - strand at 185.459 kb on + strand at 185.459 kb on + strand at 185.459 kb on + strand at 185.460 kb on - strand at 185.460 kb on - strand at 185.460 kb on - strand at 185.460 kb on - strand at 185.460 kb on - strand at 185.460 kb on - strand at 185.460 kb on - strand at 185.460 kb on - strand at 185.461 kb on + strand at 185.461 kb on + strand at 185.461 kb on + strand at 185.461 kb on + strand at 185.461 kb on + strand at 185.461 kb on + strand at 185.461 kb on + strand at 185.462 kb on - strand at 185.462 kb on - strand at 185.462 kb on - strand at 185.462 kb on - strand at 185.462 kb on - strand at 185.462 kb on - strand at 185.462 kb on - strand at 185.462 kb on - strand at 185.462 kb on - strand at 185.494 kb on + strand at 185.494 kb on + strand at 185.495 kb on - strand at 185.495 kb on - strand at 185.652 kb on - strand, within EX31_RS11265 at 185.696 kb on + strand, within EX31_RS11265 at 185.760 kb on + strand, within EX31_RS11265 at 185.865 kb on - strand, within EX31_RS11265 at 185.879 kb on + strand, within EX31_RS11265 at 185.882 kb on + strand, within EX31_RS11265 at 185.882 kb on + strand, within EX31_RS11265 at 185.883 kb on - strand, within EX31_RS11265 at 185.903 kb on - strand, within EX31_RS11265 at 185.933 kb on + strand, within EX31_RS11265 at 185.933 kb on + strand, within EX31_RS11265 at 185.933 kb on + strand, within EX31_RS11265 at 185.933 kb on + strand, within EX31_RS11265 at 185.934 kb on - strand, within EX31_RS11265 at 185.934 kb on - strand, within EX31_RS11265 at 185.934 kb on - strand, within EX31_RS11265 at 185.934 kb on - strand, within EX31_RS11265 at 185.934 kb on - strand, within EX31_RS11265 at 185.934 kb on - strand, within EX31_RS11265 at 185.934 kb on - strand, within EX31_RS11265 at 185.945 kb on + strand, within EX31_RS11265 at 185.945 kb on + strand, within EX31_RS11265 at 186.155 kb on + strand, within EX31_RS11265 at 186.156 kb on - strand, within EX31_RS11265 at 186.156 kb on - strand, within EX31_RS11265 at 186.288 kb on + strand at 186.288 kb on + strand at 186.347 kb on - strand at 186.347 kb on - strand at 186.347 kb on - strand at 186.347 kb on - strand at 186.347 kb on - strand at 186.354 kb on - strand at 186.362 kb on + strand at 186.362 kb on + strand at 186.363 kb on - strand at 186.467 kb on - strand at 186.467 kb on - strand at 186.467 kb on - strand at 186.467 kb on - strand at 186.467 kb on - strand at 186.467 kb on - strand at 186.467 kb on - strand at 186.470 kb on - strand at 186.470 kb on - strand at 186.470 kb on - strand at 186.478 kb on + strand at 186.497 kb on - strand at 186.524 kb on + strand at 186.611 kb on + strand, within EX31_RS11270 at 186.611 kb on + strand, within EX31_RS11270 at 186.611 kb on + strand, within EX31_RS11270 at 186.611 kb on + strand, within EX31_RS11270 at 186.611 kb on + strand, within EX31_RS11270 at 186.611 kb on + strand, within EX31_RS11270 at 186.612 kb on - strand, within EX31_RS11270 at 186.612 kb on - strand, within EX31_RS11270 at 186.615 kb on + strand, within EX31_RS11270 at 186.615 kb on + strand, within EX31_RS11270 at 186.616 kb on - strand, within EX31_RS11270 at 186.616 kb on - strand, within EX31_RS11270 at 186.616 kb on - strand, within EX31_RS11270 at 186.822 kb on - strand, within EX31_RS11270 at 186.933 kb on + strand, within EX31_RS11270 at 186.934 kb on - strand, within EX31_RS11270 at 186.955 kb on + strand, within EX31_RS11270 at 186.956 kb on - strand, within EX31_RS11270 at 186.963 kb on - strand, within EX31_RS11270 at 186.967 kb on + strand, within EX31_RS11270 at 186.967 kb on + strand, within EX31_RS11270 at 186.968 kb on - strand, within EX31_RS11270 at 186.999 kb on - strand, within EX31_RS11270 at 187.000 kb on + strand, within EX31_RS11270 at 187.000 kb on + strand, within EX31_RS11270 at 187.001 kb on - strand, within EX31_RS11270 at 187.001 kb on - strand, within EX31_RS11270 at 187.001 kb on - strand, within EX31_RS11270 at 187.001 kb on - strand, within EX31_RS11270
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; no shaking remove 184,417 + -1.5 184,417 + +0.1 184,417 + +0.9 184,417 + +0.4 184,417 + +2.1 184,417 + +0.2 184,417 + -1.8 184,417 + +0.3 184,417 + -1.0 184,418 - -1.7 184,419 + +0.2 184,419 + +0.3 184,419 + -0.9 184,419 + +0.8 184,419 + -2.8 184,419 + -0.5 184,419 + +1.4 184,419 + -0.0 184,420 - +0.2 184,420 - +0.8 184,420 - +0.9 184,477 + +0.1 184,477 + +0.9 184,637 + EX31_RS11260 0.33 -1.8 184,637 + EX31_RS11260 0.33 +2.0 184,637 + EX31_RS11260 0.33 -2.4 184,637 + EX31_RS11260 0.33 +0.2 184,638 - EX31_RS11260 0.33 +1.5 184,638 - EX31_RS11260 0.33 +1.1 184,684 + EX31_RS11260 0.40 -1.4 184,737 + EX31_RS11260 0.47 +1.1 184,738 - EX31_RS11260 0.47 -0.9 184,773 + EX31_RS11260 0.52 -0.8 184,831 + EX31_RS11260 0.61 -0.0 184,832 - EX31_RS11260 0.61 +1.3 184,851 + EX31_RS11260 0.64 -1.2 184,852 - EX31_RS11260 0.64 -0.5 184,852 - EX31_RS11260 0.64 +1.1 184,852 - EX31_RS11260 0.64 +0.1 184,852 - EX31_RS11260 0.64 -0.8 184,852 - EX31_RS11260 0.64 +1.2 185,144 + +0.0 185,144 + +1.3 185,144 + -0.5 185,144 + -0.8 185,179 + -1.5 185,179 + +1.2 185,179 + -0.8 185,179 + -1.5 185,179 + +0.1 185,180 - +0.1 185,184 + -0.1 185,184 + +0.1 185,184 + -0.2 185,184 + -0.4 185,185 - -1.2 185,185 - -1.0 185,188 + +1.2 185,188 + +2.3 185,188 + +0.4 185,188 + -0.8 185,188 + -2.4 185,189 - -0.7 185,189 - -1.5 185,189 - +0.7 185,189 - -1.9 185,254 + -0.1 185,254 + -1.5 185,255 - +0.4 185,255 - +1.8 185,263 + +0.2 185,263 + -0.5 185,264 - -0.2 185,284 + -1.2 185,284 + +0.0 185,284 + -1.2 185,284 + +0.8 185,285 - +0.3 185,285 - -2.0 185,285 - -0.0 185,307 + -1.0 185,312 - -0.2 185,394 - -1.4 185,394 - -1.3 185,407 + -1.8 185,408 - -1.2 185,408 - -0.5 185,435 + +1.0 185,435 + +0.2 185,435 + +2.0 185,436 - +1.0 185,436 - +1.0 185,436 - +0.8 185,436 - +1.1 185,452 + +0.2 185,453 - -0.8 185,459 + -0.5 185,459 + +0.3 185,459 + -0.5 185,460 - +1.6 185,460 - -1.4 185,460 - +2.3 185,460 - +1.8 185,460 - -1.2 185,460 - +1.4 185,460 - +0.3 185,460 - -0.0 185,461 + -0.4 185,461 + +2.1 185,461 + +1.4 185,461 + +2.8 185,461 + +1.3 185,461 + +2.5 185,461 + +0.1 185,462 - -0.8 185,462 - -0.9 185,462 - +0.6 185,462 - +1.4 185,462 - -0.5 185,462 - -0.2 185,462 - +0.6 185,462 - +0.6 185,462 - +0.5 185,494 + +1.2 185,494 + -1.1 185,495 - +1.8 185,495 - +1.1 185,652 - EX31_RS11265 0.25 +1.8 185,696 + EX31_RS11265 0.30 -1.0 185,760 + EX31_RS11265 0.36 +1.2 185,865 - EX31_RS11265 0.47 +1.4 185,879 + EX31_RS11265 0.49 +0.0 185,882 + EX31_RS11265 0.49 +1.0 185,882 + EX31_RS11265 0.49 +2.3 185,883 - EX31_RS11265 0.49 +1.4 185,903 - EX31_RS11265 0.51 -2.1 185,933 + EX31_RS11265 0.54 +0.4 185,933 + EX31_RS11265 0.54 +0.9 185,933 + EX31_RS11265 0.54 -0.5 185,933 + EX31_RS11265 0.54 +2.2 185,934 - EX31_RS11265 0.55 +0.9 185,934 - EX31_RS11265 0.55 +0.2 185,934 - EX31_RS11265 0.55 +0.7 185,934 - EX31_RS11265 0.55 +1.8 185,934 - EX31_RS11265 0.55 +0.2 185,934 - EX31_RS11265 0.55 +0.8 185,934 - EX31_RS11265 0.55 -2.7 185,945 + EX31_RS11265 0.56 +0.9 185,945 + EX31_RS11265 0.56 -1.8 186,155 + EX31_RS11265 0.77 +2.2 186,156 - EX31_RS11265 0.78 +1.6 186,156 - EX31_RS11265 0.78 +1.4 186,288 + +1.2 186,288 + +0.9 186,347 - +0.6 186,347 - -1.7 186,347 - +1.6 186,347 - -0.5 186,347 - -0.8 186,354 - +0.6 186,362 + +0.7 186,362 + +0.4 186,363 - +0.5 186,467 - -0.2 186,467 - -0.2 186,467 - -1.0 186,467 - -0.5 186,467 - +0.5 186,467 - -0.0 186,467 - -1.7 186,470 - +0.9 186,470 - +0.5 186,470 - -0.5 186,478 + +1.8 186,497 - -0.4 186,524 + +0.7 186,611 + EX31_RS11270 0.13 -1.7 186,611 + EX31_RS11270 0.13 -2.0 186,611 + EX31_RS11270 0.13 +0.7 186,611 + EX31_RS11270 0.13 +0.2 186,611 + EX31_RS11270 0.13 +3.4 186,611 + EX31_RS11270 0.13 +1.0 186,612 - EX31_RS11270 0.13 +1.2 186,612 - EX31_RS11270 0.13 -1.2 186,615 + EX31_RS11270 0.13 -0.7 186,615 + EX31_RS11270 0.13 +1.2 186,616 - EX31_RS11270 0.13 +0.2 186,616 - EX31_RS11270 0.13 +0.6 186,616 - EX31_RS11270 0.13 +0.7 186,822 - EX31_RS11270 0.26 -0.2 186,933 + EX31_RS11270 0.32 +0.4 186,934 - EX31_RS11270 0.32 +0.2 186,955 + EX31_RS11270 0.34 +0.5 186,956 - EX31_RS11270 0.34 +0.4 186,963 - EX31_RS11270 0.34 +0.9 186,967 + EX31_RS11270 0.34 -1.5 186,967 + EX31_RS11270 0.34 -2.3 186,968 - EX31_RS11270 0.34 -0.9 186,999 - EX31_RS11270 0.36 +0.0 187,000 + EX31_RS11270 0.36 -0.1 187,000 + EX31_RS11270 0.36 +0.4 187,001 - EX31_RS11270 0.36 +2.2 187,001 - EX31_RS11270 0.36 +0.8 187,001 - EX31_RS11270 0.36 +0.8 187,001 - EX31_RS11270 0.36 +1.8
Or see this region's nucleotide sequence