Experiment: L-Serine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS25995 and EX31_RS10560 are separated by 161 nucleotides EX31_RS10560 and EX31_RS10565 are separated by 107 nucleotides EX31_RS10565 and EX31_RS10570 are separated by 26 nucleotides
EX31_RS25995: EX31_RS25995 - IS3 family transposase, at 24,285 to 25,384
_RS25995
EX31_RS10560: EX31_RS10560 - YcgN family cysteine cluster protein, at 25,546 to 25,995
_RS10560
EX31_RS10565: EX31_RS10565 - fumarylacetoacetate hydrolase family protein, at 26,103 to 26,759
_RS10565
EX31_RS10570: EX31_RS10570 - lytic murein transglycosylase, at 26,786 to 27,961
_RS10570
Position (kb) 26
27 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 25.270 kb on - strand, within EX31_RS25995 at 25.353 kb on - strand at 25.366 kb on + strand at 25.391 kb on + strand at 25.392 kb on - strand at 25.435 kb on - strand at 25.493 kb on + strand at 25.493 kb on + strand at 25.494 kb on - strand at 25.494 kb on - strand at 25.494 kb on - strand at 25.494 kb on - strand at 25.496 kb on - strand at 25.496 kb on - strand at 25.496 kb on - strand at 25.548 kb on - strand at 25.566 kb on + strand at 25.619 kb on + strand, within EX31_RS10560 at 25.620 kb on - strand, within EX31_RS10560 at 25.620 kb on - strand, within EX31_RS10560 at 25.624 kb on + strand, within EX31_RS10560 at 25.624 kb on + strand, within EX31_RS10560 at 25.624 kb on + strand, within EX31_RS10560 at 25.625 kb on - strand, within EX31_RS10560 at 25.642 kb on - strand, within EX31_RS10560 at 25.642 kb on - strand, within EX31_RS10560 at 25.642 kb on - strand, within EX31_RS10560 at 25.642 kb on - strand, within EX31_RS10560 at 25.642 kb on - strand, within EX31_RS10560 at 25.774 kb on - strand, within EX31_RS10560 at 25.808 kb on + strand, within EX31_RS10560 at 25.809 kb on - strand, within EX31_RS10560 at 25.809 kb on - strand, within EX31_RS10560 at 25.812 kb on + strand, within EX31_RS10560 at 25.813 kb on - strand, within EX31_RS10560 at 25.813 kb on - strand, within EX31_RS10560 at 25.813 kb on - strand, within EX31_RS10560 at 25.813 kb on - strand, within EX31_RS10560 at 26.010 kb on - strand at 26.011 kb on + strand at 26.031 kb on - strand at 26.031 kb on - strand at 26.031 kb on - strand at 26.031 kb on - strand at 26.031 kb on - strand at 26.031 kb on - strand at 26.031 kb on - strand at 26.031 kb on - strand at 26.031 kb on - strand at 26.031 kb on - strand at 26.031 kb on - strand at 26.031 kb on - strand at 26.031 kb on - strand at 26.031 kb on - strand at 26.031 kb on - strand at 26.031 kb on - strand at 26.031 kb on - strand at 26.031 kb on - strand at 26.031 kb on - strand at 26.031 kb on - strand at 26.031 kb on - strand at 26.031 kb on - strand at 26.031 kb on - strand at 26.031 kb on - strand at 26.031 kb on - strand at 26.033 kb on - strand at 26.033 kb on - strand at 26.033 kb on - strand at 26.033 kb on - strand at 26.033 kb on - strand at 26.044 kb on - strand at 26.122 kb on - strand at 26.129 kb on + strand at 26.129 kb on + strand at 26.129 kb on + strand at 26.136 kb on - strand at 26.136 kb on - strand at 26.204 kb on + strand, within EX31_RS10565 at 26.205 kb on - strand, within EX31_RS10565 at 26.205 kb on - strand, within EX31_RS10565 at 26.237 kb on + strand, within EX31_RS10565 at 26.238 kb on - strand, within EX31_RS10565 at 26.238 kb on - strand, within EX31_RS10565 at 26.238 kb on - strand, within EX31_RS10565 at 26.239 kb on + strand, within EX31_RS10565 at 26.239 kb on + strand, within EX31_RS10565 at 26.239 kb on + strand, within EX31_RS10565 at 26.239 kb on + strand, within EX31_RS10565 at 26.240 kb on - strand, within EX31_RS10565 at 26.361 kb on - strand, within EX31_RS10565 at 26.483 kb on + strand, within EX31_RS10565 at 26.535 kb on + strand, within EX31_RS10565 at 26.536 kb on - strand, within EX31_RS10565 at 26.536 kb on - strand, within EX31_RS10565 at 26.536 kb on - strand, within EX31_RS10565 at 26.536 kb on - strand, within EX31_RS10565 at 26.628 kb on - strand, within EX31_RS10565 at 26.628 kb on - strand, within EX31_RS10565 at 26.628 kb on - strand, within EX31_RS10565 at 26.632 kb on - strand, within EX31_RS10565 at 26.632 kb on - strand, within EX31_RS10565 at 26.670 kb on - strand, within EX31_RS10565 at 26.672 kb on + strand, within EX31_RS10565 at 26.673 kb on - strand, within EX31_RS10565 at 26.683 kb on + strand, within EX31_RS10565 at 26.684 kb on - strand, within EX31_RS10565 at 26.684 kb on - strand, within EX31_RS10565 at 26.756 kb on - strand at 26.756 kb on - strand at 26.759 kb on + strand at 26.759 kb on + strand at 26.760 kb on - strand at 26.760 kb on - strand at 26.760 kb on - strand at 26.760 kb on - strand at 26.760 kb on - strand at 26.760 kb on - strand at 26.760 kb on - strand at 26.760 kb on - strand at 26.760 kb on - strand at 26.760 kb on - strand at 26.760 kb on - strand at 26.760 kb on - strand at 26.760 kb on - strand at 26.760 kb on - strand at 26.760 kb on - strand at 26.760 kb on - strand at 26.760 kb on - strand at 26.765 kb on + strand at 26.765 kb on + strand at 26.765 kb on + strand at 26.766 kb on - strand at 26.766 kb on - strand at 26.766 kb on - strand at 26.766 kb on - strand at 26.804 kb on - strand at 26.804 kb on - strand at 26.856 kb on - strand at 26.891 kb on - strand at 26.894 kb on - strand at 26.894 kb on - strand at 26.894 kb on - strand at 26.894 kb on - strand at 26.894 kb on - strand at 26.929 kb on - strand, within EX31_RS10570 at 26.929 kb on - strand, within EX31_RS10570 at 26.929 kb on - strand, within EX31_RS10570 at 26.965 kb on + strand, within EX31_RS10570 at 26.971 kb on - strand, within EX31_RS10570 at 26.971 kb on - strand, within EX31_RS10570 at 26.971 kb on - strand, within EX31_RS10570 at 27.011 kb on - strand, within EX31_RS10570 at 27.198 kb on + strand, within EX31_RS10570 at 27.199 kb on - strand, within EX31_RS10570 at 27.199 kb on - strand, within EX31_RS10570 at 27.199 kb on - strand, within EX31_RS10570 at 27.246 kb on - strand, within EX31_RS10570 at 27.246 kb on - strand, within EX31_RS10570 at 27.246 kb on - strand, within EX31_RS10570 at 27.277 kb on - strand, within EX31_RS10570 at 27.286 kb on - strand, within EX31_RS10570 at 27.286 kb on - strand, within EX31_RS10570 at 27.286 kb on - strand, within EX31_RS10570 at 27.286 kb on - strand, within EX31_RS10570 at 27.389 kb on - strand, within EX31_RS10570 at 27.452 kb on + strand, within EX31_RS10570 at 27.453 kb on - strand, within EX31_RS10570 at 27.453 kb on - strand, within EX31_RS10570 at 27.453 kb on - strand, within EX31_RS10570 at 27.453 kb on - strand, within EX31_RS10570 at 27.453 kb on - strand, within EX31_RS10570 at 27.453 kb on - strand, within EX31_RS10570 at 27.453 kb on - strand, within EX31_RS10570 at 27.513 kb on + strand, within EX31_RS10570 at 27.513 kb on + strand, within EX31_RS10570 at 27.514 kb on - strand, within EX31_RS10570 at 27.514 kb on - strand, within EX31_RS10570 at 27.514 kb on - strand, within EX31_RS10570 at 27.514 kb on - strand, within EX31_RS10570 at 27.593 kb on - strand, within EX31_RS10570 at 27.625 kb on - strand, within EX31_RS10570 at 27.625 kb on - strand, within EX31_RS10570 at 27.625 kb on - strand, within EX31_RS10570 at 27.637 kb on + strand, within EX31_RS10570 at 27.638 kb on - strand, within EX31_RS10570
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; no shaking remove 25,270 - EX31_RS25995 0.90 -2.3 25,353 - +0.8 25,366 + -0.5 25,391 + -0.1 25,392 - +0.2 25,435 - +0.8 25,493 + -0.0 25,493 + -1.0 25,494 - -0.2 25,494 - -0.3 25,494 - -0.8 25,494 - -1.0 25,496 - +1.3 25,496 - -0.2 25,496 - +1.2 25,548 - +0.9 25,566 + +0.1 25,619 + EX31_RS10560 0.16 +2.8 25,620 - EX31_RS10560 0.16 +0.0 25,620 - EX31_RS10560 0.16 +0.9 25,624 + EX31_RS10560 0.17 +1.3 25,624 + EX31_RS10560 0.17 +0.2 25,624 + EX31_RS10560 0.17 -0.0 25,625 - EX31_RS10560 0.18 +0.6 25,642 - EX31_RS10560 0.21 +1.8 25,642 - EX31_RS10560 0.21 +1.1 25,642 - EX31_RS10560 0.21 -0.8 25,642 - EX31_RS10560 0.21 +2.6 25,642 - EX31_RS10560 0.21 +0.4 25,774 - EX31_RS10560 0.51 +1.3 25,808 + EX31_RS10560 0.58 -1.1 25,809 - EX31_RS10560 0.58 -2.1 25,809 - EX31_RS10560 0.58 -0.9 25,812 + EX31_RS10560 0.59 -1.8 25,813 - EX31_RS10560 0.59 -0.5 25,813 - EX31_RS10560 0.59 -2.1 25,813 - EX31_RS10560 0.59 +1.0 25,813 - EX31_RS10560 0.59 -2.4 26,010 - -1.8 26,011 + +1.8 26,031 - +2.4 26,031 - -1.7 26,031 - +0.7 26,031 - +0.8 26,031 - +0.4 26,031 - -0.3 26,031 - -0.3 26,031 - +0.2 26,031 - +0.5 26,031 - -0.7 26,031 - -0.5 26,031 - +1.0 26,031 - -1.5 26,031 - -1.2 26,031 - -0.0 26,031 - -0.2 26,031 - +1.8 26,031 - -0.3 26,031 - +0.3 26,031 - +1.4 26,031 - -1.1 26,031 - +0.9 26,031 - +0.6 26,031 - -1.2 26,031 - -0.6 26,033 - -0.2 26,033 - +1.0 26,033 - -2.0 26,033 - +1.2 26,033 - -1.2 26,044 - +1.0 26,122 - +0.9 26,129 + -2.1 26,129 + -1.0 26,129 + -0.3 26,136 - -0.2 26,136 - -0.0 26,204 + EX31_RS10565 0.15 +0.2 26,205 - EX31_RS10565 0.16 +0.0 26,205 - EX31_RS10565 0.16 -0.0 26,237 + EX31_RS10565 0.20 -1.5 26,238 - EX31_RS10565 0.21 -2.5 26,238 - EX31_RS10565 0.21 -2.0 26,238 - EX31_RS10565 0.21 -0.5 26,239 + EX31_RS10565 0.21 +1.1 26,239 + EX31_RS10565 0.21 +0.8 26,239 + EX31_RS10565 0.21 -0.5 26,239 + EX31_RS10565 0.21 +1.5 26,240 - EX31_RS10565 0.21 -1.5 26,361 - EX31_RS10565 0.39 -1.5 26,483 + EX31_RS10565 0.58 +2.2 26,535 + EX31_RS10565 0.66 -0.8 26,536 - EX31_RS10565 0.66 -1.9 26,536 - EX31_RS10565 0.66 -0.0 26,536 - EX31_RS10565 0.66 -1.2 26,536 - EX31_RS10565 0.66 +0.2 26,628 - EX31_RS10565 0.80 -1.0 26,628 - EX31_RS10565 0.80 -0.5 26,628 - EX31_RS10565 0.80 -0.2 26,632 - EX31_RS10565 0.81 -1.2 26,632 - EX31_RS10565 0.81 +0.6 26,670 - EX31_RS10565 0.86 +0.5 26,672 + EX31_RS10565 0.87 +0.8 26,673 - EX31_RS10565 0.87 -1.4 26,683 + EX31_RS10565 0.88 -0.8 26,684 - EX31_RS10565 0.88 +0.7 26,684 - EX31_RS10565 0.88 -0.1 26,756 - -0.5 26,756 - +1.2 26,759 + -0.4 26,759 + -0.5 26,760 - -0.2 26,760 - -0.6 26,760 - -0.2 26,760 - +1.6 26,760 - +0.9 26,760 - -0.7 26,760 - -1.9 26,760 - +0.5 26,760 - +0.2 26,760 - +0.8 26,760 - +0.8 26,760 - +0.1 26,760 - -0.1 26,760 - +0.5 26,760 - +1.9 26,760 - +0.6 26,760 - -1.4 26,765 + +0.8 26,765 + -0.0 26,765 + +1.0 26,766 - +0.7 26,766 - -0.3 26,766 - -0.2 26,766 - +0.4 26,804 - +1.4 26,804 - -1.1 26,856 - +0.4 26,891 - -0.3 26,894 - +0.3 26,894 - +0.2 26,894 - +0.8 26,894 - -0.1 26,894 - -1.1 26,929 - EX31_RS10570 0.12 +0.5 26,929 - EX31_RS10570 0.12 -1.1 26,929 - EX31_RS10570 0.12 -0.2 26,965 + EX31_RS10570 0.15 +0.9 26,971 - EX31_RS10570 0.16 +0.5 26,971 - EX31_RS10570 0.16 -2.1 26,971 - EX31_RS10570 0.16 +0.3 27,011 - EX31_RS10570 0.19 +3.0 27,198 + EX31_RS10570 0.35 +0.0 27,199 - EX31_RS10570 0.35 -1.7 27,199 - EX31_RS10570 0.35 +1.6 27,199 - EX31_RS10570 0.35 -0.0 27,246 - EX31_RS10570 0.39 +0.7 27,246 - EX31_RS10570 0.39 -0.1 27,246 - EX31_RS10570 0.39 -0.3 27,277 - EX31_RS10570 0.42 +1.8 27,286 - EX31_RS10570 0.43 -0.4 27,286 - EX31_RS10570 0.43 +1.2 27,286 - EX31_RS10570 0.43 -0.5 27,286 - EX31_RS10570 0.43 -1.0 27,389 - EX31_RS10570 0.51 +3.5 27,452 + EX31_RS10570 0.57 -0.8 27,453 - EX31_RS10570 0.57 +0.6 27,453 - EX31_RS10570 0.57 -0.6 27,453 - EX31_RS10570 0.57 -0.1 27,453 - EX31_RS10570 0.57 -0.6 27,453 - EX31_RS10570 0.57 -0.1 27,453 - EX31_RS10570 0.57 -0.3 27,453 - EX31_RS10570 0.57 -0.2 27,513 + EX31_RS10570 0.62 +0.5 27,513 + EX31_RS10570 0.62 +0.5 27,514 - EX31_RS10570 0.62 +0.2 27,514 - EX31_RS10570 0.62 -0.1 27,514 - EX31_RS10570 0.62 -0.4 27,514 - EX31_RS10570 0.62 +0.2 27,593 - EX31_RS10570 0.69 +0.7 27,625 - EX31_RS10570 0.71 +0.1 27,625 - EX31_RS10570 0.71 +0.2 27,625 - EX31_RS10570 0.71 -0.1 27,637 + EX31_RS10570 0.72 +1.0 27,638 - EX31_RS10570 0.72 -0.3
Or see this region's nucleotide sequence