Experiment: L-Serine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS06745 and EX31_RS06750 overlap by 1 nucleotides EX31_RS06750 and EX31_RS06755 are separated by 123 nucleotides EX31_RS06755 and EX31_RS06760 are separated by 57 nucleotides
EX31_RS06745: EX31_RS06745 - ABC transporter permease, at 134,156 to 135,037
_RS06745
EX31_RS06750: EX31_RS06750 - ABC transporter ATP-binding protein, at 135,037 to 135,822
_RS06750
EX31_RS06755: EX31_RS06755 - histidine ammonia-lyase, at 135,946 to 137,496
_RS06755
EX31_RS06760: EX31_RS06760 - urocanate hydratase, at 137,554 to 139,242
_RS06760
Position (kb) 135
136
137
138 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 134.953 kb on + strand at 135.076 kb on - strand at 135.180 kb on + strand, within EX31_RS06750 at 135.181 kb on - strand, within EX31_RS06750 at 135.298 kb on + strand, within EX31_RS06750 at 135.299 kb on - strand, within EX31_RS06750 at 135.299 kb on - strand, within EX31_RS06750 at 135.300 kb on + strand, within EX31_RS06750 at 135.301 kb on - strand, within EX31_RS06750 at 135.394 kb on + strand, within EX31_RS06750 at 135.395 kb on - strand, within EX31_RS06750 at 135.399 kb on + strand, within EX31_RS06750 at 135.400 kb on - strand, within EX31_RS06750 at 135.402 kb on + strand, within EX31_RS06750 at 135.402 kb on + strand, within EX31_RS06750 at 135.403 kb on - strand, within EX31_RS06750 at 135.405 kb on + strand, within EX31_RS06750 at 135.405 kb on + strand, within EX31_RS06750 at 135.651 kb on + strand, within EX31_RS06750 at 135.654 kb on + strand, within EX31_RS06750 at 135.655 kb on - strand, within EX31_RS06750 at 135.730 kb on + strand, within EX31_RS06750 at 135.730 kb on + strand, within EX31_RS06750 at 135.850 kb on - strand at 135.856 kb on + strand at 135.857 kb on - strand at 135.857 kb on - strand at 135.869 kb on + strand at 135.869 kb on + strand at 135.876 kb on + strand at 135.876 kb on + strand at 135.877 kb on - strand at 135.930 kb on + strand at 135.932 kb on + strand at 136.126 kb on + strand, within EX31_RS06755 at 136.406 kb on + strand, within EX31_RS06755 at 136.406 kb on + strand, within EX31_RS06755 at 136.820 kb on + strand, within EX31_RS06755 at 137.109 kb on + strand, within EX31_RS06755 at 137.216 kb on + strand, within EX31_RS06755 at 137.216 kb on + strand, within EX31_RS06755 at 137.230 kb on + strand, within EX31_RS06755 at 137.230 kb on + strand, within EX31_RS06755 at 137.231 kb on - strand, within EX31_RS06755 at 137.263 kb on - strand, within EX31_RS06755 at 137.772 kb on - strand, within EX31_RS06760 at 137.971 kb on + strand, within EX31_RS06760 at 138.004 kb on + strand, within EX31_RS06760 at 138.004 kb on + strand, within EX31_RS06760 at 138.004 kb on + strand, within EX31_RS06760 at 138.005 kb on - strand, within EX31_RS06760 at 138.005 kb on - strand, within EX31_RS06760 at 138.037 kb on + strand, within EX31_RS06760 at 138.037 kb on + strand, within EX31_RS06760 at 138.038 kb on - strand, within EX31_RS06760 at 138.038 kb on - strand, within EX31_RS06760 at 138.135 kb on - strand, within EX31_RS06760 at 138.197 kb on - strand, within EX31_RS06760 at 138.388 kb on + strand, within EX31_RS06760
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; no shaking remove 134,953 + +1.9 135,076 - -0.4 135,180 + EX31_RS06750 0.18 +1.8 135,181 - EX31_RS06750 0.18 +1.1 135,298 + EX31_RS06750 0.33 -0.6 135,299 - EX31_RS06750 0.33 -0.1 135,299 - EX31_RS06750 0.33 -0.4 135,300 + EX31_RS06750 0.33 -0.4 135,301 - EX31_RS06750 0.34 -1.2 135,394 + EX31_RS06750 0.45 +0.9 135,395 - EX31_RS06750 0.46 +0.0 135,399 + EX31_RS06750 0.46 -1.4 135,400 - EX31_RS06750 0.46 +0.6 135,402 + EX31_RS06750 0.46 -0.2 135,402 + EX31_RS06750 0.46 +1.6 135,403 - EX31_RS06750 0.47 +1.0 135,405 + EX31_RS06750 0.47 +0.6 135,405 + EX31_RS06750 0.47 +4.6 135,651 + EX31_RS06750 0.78 +0.0 135,654 + EX31_RS06750 0.78 +1.4 135,655 - EX31_RS06750 0.79 +1.6 135,730 + EX31_RS06750 0.88 -1.0 135,730 + EX31_RS06750 0.88 -0.6 135,850 - +2.0 135,856 + -3.1 135,857 - +0.3 135,857 - +1.9 135,869 + -0.7 135,869 + +1.6 135,876 + +1.6 135,876 + +0.6 135,877 - -0.4 135,930 + -1.6 135,932 + +0.7 136,126 + EX31_RS06755 0.12 -1.0 136,406 + EX31_RS06755 0.30 -2.6 136,406 + EX31_RS06755 0.30 +0.4 136,820 + EX31_RS06755 0.56 +1.6 137,109 + EX31_RS06755 0.75 -2.0 137,216 + EX31_RS06755 0.82 +1.2 137,216 + EX31_RS06755 0.82 +0.2 137,230 + EX31_RS06755 0.83 +2.6 137,230 + EX31_RS06755 0.83 -2.0 137,231 - EX31_RS06755 0.83 -1.0 137,263 - EX31_RS06755 0.85 -1.5 137,772 - EX31_RS06760 0.13 -1.2 137,971 + EX31_RS06760 0.25 +0.6 138,004 + EX31_RS06760 0.27 -0.1 138,004 + EX31_RS06760 0.27 -0.2 138,004 + EX31_RS06760 0.27 +1.7 138,005 - EX31_RS06760 0.27 +1.1 138,005 - EX31_RS06760 0.27 +0.6 138,037 + EX31_RS06760 0.29 -2.6 138,037 + EX31_RS06760 0.29 +2.1 138,038 - EX31_RS06760 0.29 +1.4 138,038 - EX31_RS06760 0.29 -1.0 138,135 - EX31_RS06760 0.34 -0.0 138,197 - EX31_RS06760 0.38 +1.6 138,388 + EX31_RS06760 0.49 -1.1
Or see this region's nucleotide sequence