Experiment: L-Serine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS05970 and EX31_RS05975 are separated by 19 nucleotides EX31_RS05975 and EX31_RS05980 are separated by 127 nucleotides
EX31_RS05970: EX31_RS05970 - DNA topoisomerase (ATP-hydrolyzing) subunit B, at 350,704 to 353,118
_RS05970
EX31_RS05975: EX31_RS05975 - DNA replication/repair protein RecF, at 353,138 to 354,226
_RS05975
EX31_RS05980: EX31_RS05980 - DNA polymerase III subunit beta, at 354,354 to 355,454
_RS05980
Position (kb) 353
354
355 Strain fitness (log2 ratio) -2
-1
0
1
2 at 353.151 kb on - strand at 353.160 kb on - strand at 353.160 kb on - strand at 353.165 kb on - strand at 353.165 kb on - strand at 353.328 kb on + strand, within EX31_RS05975 at 353.328 kb on + strand, within EX31_RS05975 at 353.397 kb on - strand, within EX31_RS05975 at 353.397 kb on - strand, within EX31_RS05975 at 353.420 kb on + strand, within EX31_RS05975 at 353.420 kb on + strand, within EX31_RS05975 at 353.420 kb on + strand, within EX31_RS05975 at 353.421 kb on - strand, within EX31_RS05975 at 353.487 kb on + strand, within EX31_RS05975 at 353.487 kb on + strand, within EX31_RS05975 at 353.487 kb on + strand, within EX31_RS05975 at 353.487 kb on + strand, within EX31_RS05975 at 353.487 kb on + strand, within EX31_RS05975 at 353.487 kb on + strand, within EX31_RS05975 at 353.487 kb on + strand, within EX31_RS05975 at 353.487 kb on + strand, within EX31_RS05975 at 353.488 kb on - strand, within EX31_RS05975 at 353.488 kb on - strand, within EX31_RS05975 at 353.488 kb on - strand, within EX31_RS05975 at 353.488 kb on - strand, within EX31_RS05975 at 353.488 kb on - strand, within EX31_RS05975 at 353.488 kb on - strand, within EX31_RS05975 at 353.488 kb on - strand, within EX31_RS05975 at 353.488 kb on - strand, within EX31_RS05975 at 353.488 kb on - strand, within EX31_RS05975 at 353.493 kb on - strand, within EX31_RS05975 at 353.548 kb on + strand, within EX31_RS05975 at 353.640 kb on + strand, within EX31_RS05975 at 353.641 kb on - strand, within EX31_RS05975 at 353.712 kb on + strand, within EX31_RS05975 at 353.753 kb on + strand, within EX31_RS05975 at 353.818 kb on + strand, within EX31_RS05975 at 353.818 kb on + strand, within EX31_RS05975 at 353.818 kb on + strand, within EX31_RS05975 at 353.819 kb on - strand, within EX31_RS05975 at 353.819 kb on - strand, within EX31_RS05975 at 353.819 kb on - strand, within EX31_RS05975 at 353.939 kb on + strand, within EX31_RS05975 at 353.940 kb on - strand, within EX31_RS05975 at 353.940 kb on - strand, within EX31_RS05975 at 353.940 kb on - strand, within EX31_RS05975 at 353.940 kb on - strand, within EX31_RS05975 at 353.940 kb on - strand, within EX31_RS05975 at 353.945 kb on - strand, within EX31_RS05975 at 353.945 kb on - strand, within EX31_RS05975 at 353.945 kb on - strand, within EX31_RS05975 at 353.945 kb on - strand, within EX31_RS05975 at 353.945 kb on - strand, within EX31_RS05975 at 354.094 kb on + strand, within EX31_RS05975 at 354.094 kb on + strand, within EX31_RS05975 at 354.094 kb on + strand, within EX31_RS05975 at 354.095 kb on - strand, within EX31_RS05975 at 354.095 kb on - strand, within EX31_RS05975 at 354.095 kb on - strand, within EX31_RS05975 at 354.096 kb on + strand, within EX31_RS05975 at 354.096 kb on + strand, within EX31_RS05975 at 354.097 kb on - strand, within EX31_RS05975 at 354.097 kb on - strand, within EX31_RS05975 at 354.097 kb on - strand, within EX31_RS05975 at 354.097 kb on - strand, within EX31_RS05975 at 354.097 kb on - strand, within EX31_RS05975 at 354.122 kb on - strand at 354.128 kb on - strand at 354.152 kb on - strand at 354.170 kb on + strand at 354.203 kb on + strand at 354.203 kb on + strand at 354.204 kb on - strand at 354.209 kb on + strand at 354.209 kb on + strand at 354.210 kb on - strand at 354.210 kb on - strand at 354.210 kb on - strand at 354.210 kb on - strand at 354.228 kb on + strand at 354.228 kb on + strand at 354.228 kb on + strand at 354.228 kb on + strand at 354.228 kb on + strand at 354.228 kb on + strand at 354.228 kb on + strand at 354.228 kb on + strand at 354.228 kb on + strand at 354.228 kb on + strand at 354.228 kb on + strand at 354.228 kb on + strand at 354.228 kb on + strand at 354.228 kb on + strand at 354.228 kb on + strand at 354.228 kb on + strand at 354.228 kb on + strand at 354.228 kb on + strand at 354.228 kb on + strand at 354.229 kb on - strand at 354.229 kb on - strand at 354.229 kb on - strand at 354.229 kb on - strand at 354.229 kb on - strand at 354.229 kb on - strand at 354.229 kb on - strand at 354.229 kb on - strand at 354.229 kb on - strand at 354.229 kb on - strand at 354.229 kb on - strand at 354.229 kb on - strand at 354.229 kb on - strand at 354.229 kb on - strand at 354.229 kb on - strand at 354.229 kb on - strand at 354.229 kb on - strand at 354.229 kb on - strand at 354.229 kb on - strand at 354.229 kb on - strand at 354.229 kb on - strand at 354.229 kb on - strand at 354.232 kb on - strand at 354.275 kb on + strand at 354.275 kb on + strand at 354.275 kb on + strand at 354.276 kb on - strand at 354.276 kb on - strand at 354.276 kb on - strand at 354.276 kb on - strand at 354.276 kb on - strand at 354.276 kb on - strand at 354.281 kb on - strand at 354.291 kb on + strand at 354.291 kb on + strand at 354.291 kb on + strand at 354.292 kb on - strand at 354.355 kb on + strand at 354.355 kb on + strand at 354.355 kb on + strand at 354.356 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; no shaking remove 353,151 - +1.0 353,160 - +1.6 353,160 - -0.6 353,165 - -0.7 353,165 - +0.8 353,328 + EX31_RS05975 0.17 -1.1 353,328 + EX31_RS05975 0.17 -1.1 353,397 - EX31_RS05975 0.24 +0.2 353,397 - EX31_RS05975 0.24 +0.3 353,420 + EX31_RS05975 0.26 +0.7 353,420 + EX31_RS05975 0.26 -0.2 353,420 + EX31_RS05975 0.26 +1.8 353,421 - EX31_RS05975 0.26 -0.4 353,487 + EX31_RS05975 0.32 -0.5 353,487 + EX31_RS05975 0.32 +0.9 353,487 + EX31_RS05975 0.32 +0.6 353,487 + EX31_RS05975 0.32 -1.0 353,487 + EX31_RS05975 0.32 -0.5 353,487 + EX31_RS05975 0.32 -1.3 353,487 + EX31_RS05975 0.32 +1.5 353,487 + EX31_RS05975 0.32 +0.1 353,488 - EX31_RS05975 0.32 +0.6 353,488 - EX31_RS05975 0.32 +0.1 353,488 - EX31_RS05975 0.32 -1.1 353,488 - EX31_RS05975 0.32 +0.4 353,488 - EX31_RS05975 0.32 +0.4 353,488 - EX31_RS05975 0.32 -0.3 353,488 - EX31_RS05975 0.32 +0.9 353,488 - EX31_RS05975 0.32 +0.0 353,488 - EX31_RS05975 0.32 -0.4 353,493 - EX31_RS05975 0.33 -0.2 353,548 + EX31_RS05975 0.38 -0.7 353,640 + EX31_RS05975 0.46 +0.6 353,641 - EX31_RS05975 0.46 -0.4 353,712 + EX31_RS05975 0.53 +0.2 353,753 + EX31_RS05975 0.56 -1.1 353,818 + EX31_RS05975 0.62 -0.7 353,818 + EX31_RS05975 0.62 -0.7 353,818 + EX31_RS05975 0.62 +0.9 353,819 - EX31_RS05975 0.63 -1.8 353,819 - EX31_RS05975 0.63 +0.1 353,819 - EX31_RS05975 0.63 +0.1 353,939 + EX31_RS05975 0.74 -1.1 353,940 - EX31_RS05975 0.74 -0.8 353,940 - EX31_RS05975 0.74 +0.7 353,940 - EX31_RS05975 0.74 +0.1 353,940 - EX31_RS05975 0.74 -0.5 353,940 - EX31_RS05975 0.74 -0.2 353,945 - EX31_RS05975 0.74 -0.6 353,945 - EX31_RS05975 0.74 -0.3 353,945 - EX31_RS05975 0.74 -0.3 353,945 - EX31_RS05975 0.74 +1.7 353,945 - EX31_RS05975 0.74 +1.5 354,094 + EX31_RS05975 0.88 -2.0 354,094 + EX31_RS05975 0.88 -1.4 354,094 + EX31_RS05975 0.88 -1.8 354,095 - EX31_RS05975 0.88 -0.5 354,095 - EX31_RS05975 0.88 +1.2 354,095 - EX31_RS05975 0.88 -0.2 354,096 + EX31_RS05975 0.88 -1.3 354,096 + EX31_RS05975 0.88 +0.4 354,097 - EX31_RS05975 0.88 +0.1 354,097 - EX31_RS05975 0.88 +0.8 354,097 - EX31_RS05975 0.88 +0.9 354,097 - EX31_RS05975 0.88 +0.0 354,097 - EX31_RS05975 0.88 -0.2 354,122 - -1.5 354,128 - +0.3 354,152 - +0.6 354,170 + +0.3 354,203 + +0.4 354,203 + -1.6 354,204 - -1.9 354,209 + -0.1 354,209 + -1.0 354,210 - +1.7 354,210 - +0.6 354,210 - +0.7 354,210 - +0.4 354,228 + +0.9 354,228 + -0.4 354,228 + +0.8 354,228 + +0.2 354,228 + -0.2 354,228 + -2.2 354,228 + -1.2 354,228 + +1.2 354,228 + +0.4 354,228 + +0.3 354,228 + +0.2 354,228 + +0.1 354,228 + -0.9 354,228 + -0.8 354,228 + -0.5 354,228 + +0.2 354,228 + +0.2 354,228 + -0.8 354,228 + -0.3 354,229 - +0.4 354,229 - +0.4 354,229 - -0.7 354,229 - +0.8 354,229 - +0.2 354,229 - -0.2 354,229 - +0.6 354,229 - +0.5 354,229 - -1.0 354,229 - -0.0 354,229 - -0.5 354,229 - +1.4 354,229 - -0.8 354,229 - +0.3 354,229 - -1.4 354,229 - -0.5 354,229 - +0.9 354,229 - +0.2 354,229 - -0.7 354,229 - -0.2 354,229 - -0.2 354,229 - -1.5 354,232 - -1.4 354,275 + -0.5 354,275 + +0.4 354,275 + -0.4 354,276 - +1.6 354,276 - -0.8 354,276 - +0.8 354,276 - +0.4 354,276 - -0.6 354,276 - +0.7 354,281 - +1.0 354,291 + +0.4 354,291 + -0.0 354,291 + -2.3 354,292 - +0.4 354,355 + -0.2 354,355 + -0.4 354,355 + -0.8 354,356 - -0.7
Or see this region's nucleotide sequence