Experiment: L-Serine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS05515 and EX31_RS25925 are separated by 405 nucleotides EX31_RS25925 and EX31_RS05520 are separated by 89 nucleotides EX31_RS05520 and EX31_RS05525 are separated by 257 nucleotides
EX31_RS05515: EX31_RS05515 - ABC transporter substrate-binding protein, at 255,312 to 256,919
_RS05515
EX31_RS25925: EX31_RS25925 - hypothetical protein, at 257,325 to 257,558
_RS25925
EX31_RS05520: EX31_RS05520 - LysR family transcriptional regulator, at 257,648 to 258,574
_RS05520
EX31_RS05525: EX31_RS05525 - amidohydrolase, at 258,832 to 260,271
_RS05525
Position (kb) 257
258
259 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 256.648 kb on + strand, within EX31_RS05515 at 256.648 kb on + strand, within EX31_RS05515 at 256.648 kb on + strand, within EX31_RS05515 at 256.649 kb on - strand, within EX31_RS05515 at 256.649 kb on - strand, within EX31_RS05515 at 256.649 kb on - strand, within EX31_RS05515 at 256.649 kb on - strand, within EX31_RS05515 at 256.649 kb on - strand, within EX31_RS05515 at 256.738 kb on + strand, within EX31_RS05515 at 256.738 kb on + strand, within EX31_RS05515 at 256.738 kb on + strand, within EX31_RS05515 at 256.738 kb on + strand, within EX31_RS05515 at 256.738 kb on + strand, within EX31_RS05515 at 256.738 kb on + strand, within EX31_RS05515 at 256.738 kb on + strand, within EX31_RS05515 at 256.738 kb on + strand, within EX31_RS05515 at 256.739 kb on - strand, within EX31_RS05515 at 256.739 kb on - strand, within EX31_RS05515 at 256.739 kb on - strand, within EX31_RS05515 at 256.742 kb on + strand, within EX31_RS05515 at 256.742 kb on + strand, within EX31_RS05515 at 256.742 kb on + strand, within EX31_RS05515 at 256.742 kb on + strand, within EX31_RS05515 at 256.743 kb on - strand, within EX31_RS05515 at 256.762 kb on + strand at 256.762 kb on + strand at 256.762 kb on + strand at 256.762 kb on + strand at 256.762 kb on + strand at 256.762 kb on + strand at 256.762 kb on + strand at 256.762 kb on + strand at 256.763 kb on - strand at 256.763 kb on - strand at 256.763 kb on - strand at 256.763 kb on - strand at 256.763 kb on - strand at 256.763 kb on - strand at 256.822 kb on + strand at 256.822 kb on + strand at 256.822 kb on + strand at 256.822 kb on + strand at 256.822 kb on + strand at 256.822 kb on + strand at 256.822 kb on + strand at 256.822 kb on + strand at 256.823 kb on - strand at 256.823 kb on - strand at 256.823 kb on - strand at 256.823 kb on - strand at 256.823 kb on - strand at 256.823 kb on - strand at 256.827 kb on + strand at 256.877 kb on + strand at 256.901 kb on + strand at 256.901 kb on + strand at 256.902 kb on - strand at 256.905 kb on + strand at 256.906 kb on - strand at 256.906 kb on - strand at 256.906 kb on - strand at 256.939 kb on - strand at 256.939 kb on - strand at 256.966 kb on + strand at 256.966 kb on + strand at 256.966 kb on + strand at 256.966 kb on + strand at 256.966 kb on + strand at 256.966 kb on + strand at 256.967 kb on - strand at 256.967 kb on - strand at 256.967 kb on - strand at 256.967 kb on - strand at 256.967 kb on - strand at 256.967 kb on - strand at 256.967 kb on - strand at 256.967 kb on - strand at 256.967 kb on - strand at 256.968 kb on + strand at 256.968 kb on + strand at 256.968 kb on + strand at 256.968 kb on + strand at 256.968 kb on + strand at 256.968 kb on + strand at 256.969 kb on - strand at 256.969 kb on - strand at 256.969 kb on - strand at 256.969 kb on - strand at 256.969 kb on - strand at 256.969 kb on - strand at 256.994 kb on - strand at 257.029 kb on + strand at 257.029 kb on + strand at 257.029 kb on + strand at 257.029 kb on + strand at 257.029 kb on + strand at 257.029 kb on + strand at 257.030 kb on - strand at 257.030 kb on - strand at 257.030 kb on - strand at 257.030 kb on - strand at 257.030 kb on - strand at 257.030 kb on - strand at 257.030 kb on - strand at 257.108 kb on + strand at 257.109 kb on - strand at 257.164 kb on + strand at 257.164 kb on + strand at 257.164 kb on + strand at 257.164 kb on + strand at 257.165 kb on - strand at 257.165 kb on - strand at 257.165 kb on - strand at 257.166 kb on + strand at 257.166 kb on + strand at 257.166 kb on + strand at 257.166 kb on + strand at 257.166 kb on + strand at 257.166 kb on + strand at 257.166 kb on + strand at 257.166 kb on + strand at 257.166 kb on + strand at 257.167 kb on - strand at 257.167 kb on - strand at 257.167 kb on - strand at 257.167 kb on - strand at 257.181 kb on + strand at 257.181 kb on + strand at 257.182 kb on - strand at 257.182 kb on - strand at 257.266 kb on + strand at 257.267 kb on - strand at 257.286 kb on + strand at 257.287 kb on - strand at 257.287 kb on - strand at 257.287 kb on - strand at 257.318 kb on + strand at 257.318 kb on + strand at 257.351 kb on + strand, within EX31_RS25925 at 257.351 kb on + strand, within EX31_RS25925 at 257.351 kb on + strand, within EX31_RS25925 at 257.351 kb on + strand, within EX31_RS25925 at 257.351 kb on + strand, within EX31_RS25925 at 257.351 kb on + strand, within EX31_RS25925 at 257.351 kb on + strand, within EX31_RS25925 at 257.351 kb on + strand, within EX31_RS25925 at 257.351 kb on + strand, within EX31_RS25925 at 257.351 kb on + strand, within EX31_RS25925 at 257.351 kb on + strand, within EX31_RS25925 at 257.352 kb on - strand, within EX31_RS25925 at 257.352 kb on - strand, within EX31_RS25925 at 257.352 kb on - strand, within EX31_RS25925 at 257.352 kb on - strand, within EX31_RS25925 at 257.352 kb on - strand, within EX31_RS25925 at 257.352 kb on - strand, within EX31_RS25925 at 257.352 kb on - strand, within EX31_RS25925 at 257.352 kb on - strand, within EX31_RS25925 at 257.353 kb on + strand, within EX31_RS25925 at 257.353 kb on + strand, within EX31_RS25925 at 257.353 kb on + strand, within EX31_RS25925 at 257.353 kb on + strand, within EX31_RS25925 at 257.353 kb on + strand, within EX31_RS25925 at 257.353 kb on + strand, within EX31_RS25925 at 257.354 kb on - strand, within EX31_RS25925 at 257.354 kb on - strand, within EX31_RS25925 at 257.354 kb on - strand, within EX31_RS25925 at 257.354 kb on - strand, within EX31_RS25925 at 257.375 kb on - strand, within EX31_RS25925 at 257.448 kb on + strand, within EX31_RS25925 at 257.448 kb on + strand, within EX31_RS25925 at 257.448 kb on + strand, within EX31_RS25925 at 257.448 kb on + strand, within EX31_RS25925 at 257.472 kb on + strand, within EX31_RS25925 at 257.473 kb on - strand, within EX31_RS25925 at 257.505 kb on + strand, within EX31_RS25925 at 257.506 kb on - strand, within EX31_RS25925 at 257.506 kb on - strand, within EX31_RS25925 at 257.536 kb on + strand at 257.573 kb on - strand at 257.578 kb on - strand at 257.605 kb on - strand at 257.605 kb on - strand at 257.614 kb on + strand at 257.615 kb on - strand at 257.615 kb on - strand at 257.625 kb on + strand at 257.626 kb on - strand at 257.626 kb on - strand at 257.693 kb on + strand at 257.693 kb on + strand at 257.694 kb on - strand at 257.694 kb on - strand at 257.694 kb on - strand at 257.694 kb on - strand at 257.695 kb on + strand at 257.695 kb on + strand at 257.695 kb on + strand at 257.696 kb on - strand at 257.702 kb on - strand at 257.755 kb on + strand, within EX31_RS05520 at 257.755 kb on + strand, within EX31_RS05520 at 257.755 kb on + strand, within EX31_RS05520 at 257.755 kb on + strand, within EX31_RS05520 at 257.756 kb on - strand, within EX31_RS05520 at 257.756 kb on - strand, within EX31_RS05520 at 257.811 kb on + strand, within EX31_RS05520 at 257.845 kb on + strand, within EX31_RS05520 at 257.944 kb on + strand, within EX31_RS05520 at 257.944 kb on + strand, within EX31_RS05520 at 258.008 kb on + strand, within EX31_RS05520 at 258.008 kb on + strand, within EX31_RS05520 at 258.008 kb on + strand, within EX31_RS05520 at 258.009 kb on - strand, within EX31_RS05520 at 258.049 kb on + strand, within EX31_RS05520 at 258.049 kb on + strand, within EX31_RS05520 at 258.049 kb on + strand, within EX31_RS05520 at 258.049 kb on + strand, within EX31_RS05520 at 258.050 kb on - strand, within EX31_RS05520 at 258.092 kb on + strand, within EX31_RS05520 at 258.092 kb on + strand, within EX31_RS05520 at 258.092 kb on + strand, within EX31_RS05520 at 258.092 kb on + strand, within EX31_RS05520 at 258.092 kb on + strand, within EX31_RS05520 at 258.093 kb on - strand, within EX31_RS05520 at 258.093 kb on - strand, within EX31_RS05520 at 258.093 kb on - strand, within EX31_RS05520 at 258.140 kb on + strand, within EX31_RS05520 at 258.140 kb on + strand, within EX31_RS05520 at 258.141 kb on - strand, within EX31_RS05520 at 258.141 kb on - strand, within EX31_RS05520 at 258.141 kb on - strand, within EX31_RS05520 at 258.142 kb on + strand, within EX31_RS05520 at 258.248 kb on + strand, within EX31_RS05520 at 258.249 kb on - strand, within EX31_RS05520 at 258.258 kb on - strand, within EX31_RS05520 at 258.332 kb on + strand, within EX31_RS05520 at 258.333 kb on - strand, within EX31_RS05520 at 258.656 kb on - strand at 258.701 kb on + strand at 258.799 kb on + strand at 258.799 kb on + strand at 258.800 kb on - strand at 258.800 kb on - strand at 258.855 kb on + strand at 258.855 kb on + strand at 258.856 kb on - strand at 258.856 kb on - strand at 258.943 kb on + strand at 258.943 kb on + strand at 258.943 kb on + strand at 258.944 kb on - strand at 258.944 kb on - strand at 258.944 kb on - strand at 258.946 kb on - strand at 259.087 kb on + strand, within EX31_RS05525 at 259.087 kb on + strand, within EX31_RS05525 at 259.087 kb on + strand, within EX31_RS05525 at 259.209 kb on + strand, within EX31_RS05525 at 259.209 kb on + strand, within EX31_RS05525 at 259.210 kb on - strand, within EX31_RS05525 at 259.210 kb on - strand, within EX31_RS05525 at 259.261 kb on + strand, within EX31_RS05525 at 259.262 kb on - strand, within EX31_RS05525 at 259.264 kb on + strand, within EX31_RS05525 at 259.264 kb on + strand, within EX31_RS05525 at 259.510 kb on + strand, within EX31_RS05525 at 259.510 kb on + strand, within EX31_RS05525 at 259.510 kb on + strand, within EX31_RS05525 at 259.511 kb on - strand, within EX31_RS05525 at 259.511 kb on - strand, within EX31_RS05525 at 259.511 kb on - strand, within EX31_RS05525 at 259.511 kb on - strand, within EX31_RS05525 at 259.511 kb on - strand, within EX31_RS05525 at 259.527 kb on - strand, within EX31_RS05525 at 259.527 kb on - strand, within EX31_RS05525 at 259.550 kb on - strand, within EX31_RS05525
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); RCH2 media; no shaking remove 256,648 + EX31_RS05515 0.83 +0.2 256,648 + EX31_RS05515 0.83 -0.3 256,648 + EX31_RS05515 0.83 +0.1 256,649 - EX31_RS05515 0.83 -0.0 256,649 - EX31_RS05515 0.83 +1.5 256,649 - EX31_RS05515 0.83 +1.5 256,649 - EX31_RS05515 0.83 +0.0 256,649 - EX31_RS05515 0.83 -0.4 256,738 + EX31_RS05515 0.89 +0.2 256,738 + EX31_RS05515 0.89 -0.1 256,738 + EX31_RS05515 0.89 -0.1 256,738 + EX31_RS05515 0.89 +1.0 256,738 + EX31_RS05515 0.89 -1.4 256,738 + EX31_RS05515 0.89 -0.2 256,738 + EX31_RS05515 0.89 -0.9 256,738 + EX31_RS05515 0.89 +1.0 256,739 - EX31_RS05515 0.89 +1.0 256,739 - EX31_RS05515 0.89 -0.1 256,739 - EX31_RS05515 0.89 -0.7 256,742 + EX31_RS05515 0.89 -0.3 256,742 + EX31_RS05515 0.89 -1.8 256,742 + EX31_RS05515 0.89 -0.6 256,742 + EX31_RS05515 0.89 -1.0 256,743 - EX31_RS05515 0.89 -0.2 256,762 + +2.1 256,762 + -0.5 256,762 + +0.5 256,762 + +0.7 256,762 + -1.1 256,762 + +0.7 256,762 + +0.3 256,762 + +0.5 256,763 - +0.6 256,763 - -0.1 256,763 - +0.1 256,763 - +1.0 256,763 - +1.7 256,763 - +0.0 256,822 + -0.1 256,822 + +0.3 256,822 + -0.1 256,822 + -1.3 256,822 + -0.7 256,822 + -2.7 256,822 + -0.2 256,822 + -1.6 256,823 - +2.3 256,823 - +0.1 256,823 - -2.9 256,823 - +0.2 256,823 - +1.0 256,823 - +1.4 256,827 + +0.0 256,877 + +0.6 256,901 + -0.2 256,901 + -0.1 256,902 - -0.0 256,905 + -0.4 256,906 - +0.3 256,906 - -0.9 256,906 - +0.9 256,939 - -0.0 256,939 - -0.4 256,966 + -1.1 256,966 + -0.2 256,966 + -0.2 256,966 + +0.6 256,966 + +0.3 256,966 + -1.0 256,967 - +1.1 256,967 - +0.3 256,967 - +0.3 256,967 - +0.4 256,967 - +1.4 256,967 - -0.4 256,967 - +0.3 256,967 - +0.6 256,967 - -2.2 256,968 + +0.3 256,968 + -1.3 256,968 + +0.7 256,968 + +0.1 256,968 + -0.3 256,968 + -0.0 256,969 - -0.1 256,969 - +0.2 256,969 - +0.8 256,969 - +0.2 256,969 - +0.9 256,969 - +0.9 256,994 - -0.6 257,029 + +1.4 257,029 + -0.9 257,029 + +0.0 257,029 + +0.4 257,029 + +0.0 257,029 + -0.5 257,030 - +1.4 257,030 - +0.1 257,030 - +1.3 257,030 - +1.2 257,030 - +0.8 257,030 - +0.1 257,030 - -0.1 257,108 + +0.4 257,109 - +1.4 257,164 + +0.3 257,164 + +0.8 257,164 + +0.4 257,164 + +0.1 257,165 - +0.7 257,165 - +0.1 257,165 - +0.7 257,166 + -0.1 257,166 + +1.2 257,166 + -0.2 257,166 + -0.5 257,166 + -0.6 257,166 + +0.1 257,166 + +0.3 257,166 + -2.5 257,166 + +0.0 257,167 - -2.1 257,167 - +0.0 257,167 - -0.3 257,167 - -0.5 257,181 + +1.3 257,181 + +0.4 257,182 - -0.0 257,182 - -0.1 257,266 + +1.0 257,267 - -0.4 257,286 + -1.2 257,287 - +0.1 257,287 - -2.1 257,287 - +1.1 257,318 + -0.1 257,318 + -0.6 257,351 + EX31_RS25925 0.11 -1.0 257,351 + EX31_RS25925 0.11 +0.1 257,351 + EX31_RS25925 0.11 +0.6 257,351 + EX31_RS25925 0.11 -1.3 257,351 + EX31_RS25925 0.11 +1.4 257,351 + EX31_RS25925 0.11 +0.8 257,351 + EX31_RS25925 0.11 +0.3 257,351 + EX31_RS25925 0.11 -0.1 257,351 + EX31_RS25925 0.11 +1.1 257,351 + EX31_RS25925 0.11 -1.9 257,351 + EX31_RS25925 0.11 -1.0 257,352 - EX31_RS25925 0.12 -0.4 257,352 - EX31_RS25925 0.12 -0.4 257,352 - EX31_RS25925 0.12 -0.0 257,352 - EX31_RS25925 0.12 -2.0 257,352 - EX31_RS25925 0.12 -1.1 257,352 - EX31_RS25925 0.12 +1.3 257,352 - EX31_RS25925 0.12 +0.0 257,352 - EX31_RS25925 0.12 -0.5 257,353 + EX31_RS25925 0.12 +0.1 257,353 + EX31_RS25925 0.12 -2.0 257,353 + EX31_RS25925 0.12 +0.3 257,353 + EX31_RS25925 0.12 +0.6 257,353 + EX31_RS25925 0.12 -1.1 257,353 + EX31_RS25925 0.12 +0.0 257,354 - EX31_RS25925 0.12 +1.0 257,354 - EX31_RS25925 0.12 -0.1 257,354 - EX31_RS25925 0.12 -1.3 257,354 - EX31_RS25925 0.12 +0.3 257,375 - EX31_RS25925 0.21 -1.9 257,448 + EX31_RS25925 0.53 +0.1 257,448 + EX31_RS25925 0.53 -0.5 257,448 + EX31_RS25925 0.53 +0.2 257,448 + EX31_RS25925 0.53 -0.4 257,472 + EX31_RS25925 0.63 +0.3 257,473 - EX31_RS25925 0.63 +0.5 257,505 + EX31_RS25925 0.77 -0.9 257,506 - EX31_RS25925 0.77 -0.4 257,506 - EX31_RS25925 0.77 -0.2 257,536 + -0.2 257,573 - -1.2 257,578 - -0.5 257,605 - -0.2 257,605 - +0.8 257,614 + -0.3 257,615 - -0.7 257,615 - +0.2 257,625 + -1.8 257,626 - -1.0 257,626 - +0.5 257,693 + -0.4 257,693 + +0.9 257,694 - +1.7 257,694 - +0.2 257,694 - +1.4 257,694 - +0.2 257,695 + +0.1 257,695 + +1.9 257,695 + -0.4 257,696 - -0.8 257,702 - +0.4 257,755 + EX31_RS05520 0.12 -0.1 257,755 + EX31_RS05520 0.12 +1.7 257,755 + EX31_RS05520 0.12 +0.2 257,755 + EX31_RS05520 0.12 +0.7 257,756 - EX31_RS05520 0.12 -0.4 257,756 - EX31_RS05520 0.12 +1.5 257,811 + EX31_RS05520 0.18 +0.4 257,845 + EX31_RS05520 0.21 +0.6 257,944 + EX31_RS05520 0.32 +0.1 257,944 + EX31_RS05520 0.32 -0.4 258,008 + EX31_RS05520 0.39 -0.1 258,008 + EX31_RS05520 0.39 +1.2 258,008 + EX31_RS05520 0.39 +0.4 258,009 - EX31_RS05520 0.39 -0.3 258,049 + EX31_RS05520 0.43 -0.3 258,049 + EX31_RS05520 0.43 +0.9 258,049 + EX31_RS05520 0.43 +0.8 258,049 + EX31_RS05520 0.43 +0.3 258,050 - EX31_RS05520 0.43 +1.3 258,092 + EX31_RS05520 0.48 -0.7 258,092 + EX31_RS05520 0.48 +0.5 258,092 + EX31_RS05520 0.48 -0.9 258,092 + EX31_RS05520 0.48 +1.1 258,092 + EX31_RS05520 0.48 +0.5 258,093 - EX31_RS05520 0.48 -0.0 258,093 - EX31_RS05520 0.48 -1.2 258,093 - EX31_RS05520 0.48 +0.1 258,140 + EX31_RS05520 0.53 +0.1 258,140 + EX31_RS05520 0.53 +0.5 258,141 - EX31_RS05520 0.53 -0.1 258,141 - EX31_RS05520 0.53 +0.6 258,141 - EX31_RS05520 0.53 -0.1 258,142 + EX31_RS05520 0.53 +0.4 258,248 + EX31_RS05520 0.65 -0.7 258,249 - EX31_RS05520 0.65 -1.3 258,258 - EX31_RS05520 0.66 +0.7 258,332 + EX31_RS05520 0.74 +1.7 258,333 - EX31_RS05520 0.74 +0.1 258,656 - +0.0 258,701 + +0.6 258,799 + +0.1 258,799 + +0.1 258,800 - +0.6 258,800 - -0.1 258,855 + +0.4 258,855 + +0.3 258,856 - -1.0 258,856 - +2.7 258,943 + -1.3 258,943 + -0.0 258,943 + +0.4 258,944 - +0.1 258,944 - +0.9 258,944 - -0.9 258,946 - -0.6 259,087 + EX31_RS05525 0.18 -0.1 259,087 + EX31_RS05525 0.18 -0.6 259,087 + EX31_RS05525 0.18 +1.1 259,209 + EX31_RS05525 0.26 -0.9 259,209 + EX31_RS05525 0.26 +0.3 259,210 - EX31_RS05525 0.26 +0.5 259,210 - EX31_RS05525 0.26 -0.3 259,261 + EX31_RS05525 0.30 +0.0 259,262 - EX31_RS05525 0.30 +0.1 259,264 + EX31_RS05525 0.30 +0.1 259,264 + EX31_RS05525 0.30 +1.3 259,510 + EX31_RS05525 0.47 +0.6 259,510 + EX31_RS05525 0.47 -1.5 259,510 + EX31_RS05525 0.47 +1.0 259,511 - EX31_RS05525 0.47 -1.0 259,511 - EX31_RS05525 0.47 -0.2 259,511 - EX31_RS05525 0.47 +0.5 259,511 - EX31_RS05525 0.47 -0.7 259,511 - EX31_RS05525 0.47 -0.4 259,527 - EX31_RS05525 0.48 -0.7 259,527 - EX31_RS05525 0.48 +0.8 259,550 - EX31_RS05525 0.50 -0.9
Or see this region's nucleotide sequence