Experiment: Putrescine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_02222 and CCNA_02223 overlap by 4 nucleotides CCNA_02223 and CCNA_02224 overlap by 4 nucleotides CCNA_02224 and CCNA_02225 overlap by 4 nucleotides
CCNA_02222: CCNA_02222 - 5-methyltetrahydrofolate--homocysteine methyltransferase homocysteine-binding subunit, at 2,370,800 to 2,371,876
_02222
CCNA_02223: CCNA_02223 - beta-lactamase, type II, at 2,371,873 to 2,372,742
_02223
CCNA_02224: CCNA_02224 - methylenetetrahydrofolate reductase, at 2,372,739 to 2,373,683
_02224
CCNA_02225: CCNA_02225 - ArsR family/SAM-dependent transcriptional regulator, at 2,373,680 to 2,374,657
_02225
Position (kb)
2372
2373
2374 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 2371.755 kb on + strand, within CCNA_02222 at 2371.763 kb on - strand, within CCNA_02222 at 2371.827 kb on - strand at 2371.838 kb on - strand at 2371.840 kb on - strand at 2371.915 kb on + strand at 2371.937 kb on + strand at 2372.003 kb on - strand, within CCNA_02223 at 2372.009 kb on + strand, within CCNA_02223 at 2372.020 kb on + strand, within CCNA_02223 at 2372.028 kb on - strand, within CCNA_02223 at 2372.051 kb on - strand, within CCNA_02223 at 2372.063 kb on + strand, within CCNA_02223 at 2372.071 kb on - strand, within CCNA_02223 at 2372.078 kb on + strand, within CCNA_02223 at 2372.117 kb on - strand, within CCNA_02223 at 2372.123 kb on + strand, within CCNA_02223 at 2372.177 kb on + strand, within CCNA_02223 at 2372.177 kb on + strand, within CCNA_02223 at 2372.210 kb on - strand, within CCNA_02223 at 2372.211 kb on - strand, within CCNA_02223 at 2372.225 kb on + strand, within CCNA_02223 at 2372.276 kb on - strand, within CCNA_02223 at 2372.276 kb on - strand, within CCNA_02223 at 2372.276 kb on - strand, within CCNA_02223 at 2372.276 kb on - strand, within CCNA_02223 at 2372.276 kb on - strand, within CCNA_02223 at 2372.276 kb on - strand, within CCNA_02223 at 2372.281 kb on - strand, within CCNA_02223 at 2372.281 kb on - strand, within CCNA_02223 at 2372.296 kb on - strand, within CCNA_02223 at 2372.390 kb on + strand, within CCNA_02223 at 2372.390 kb on + strand, within CCNA_02223 at 2372.405 kb on - strand, within CCNA_02223 at 2372.407 kb on + strand, within CCNA_02223 at 2372.414 kb on + strand, within CCNA_02223 at 2372.414 kb on + strand, within CCNA_02223 at 2372.422 kb on - strand, within CCNA_02223 at 2372.422 kb on - strand, within CCNA_02223 at 2372.422 kb on - strand, within CCNA_02223 at 2372.422 kb on - strand, within CCNA_02223 at 2372.472 kb on - strand, within CCNA_02223 at 2372.501 kb on + strand, within CCNA_02223 at 2372.502 kb on - strand, within CCNA_02223 at 2372.559 kb on - strand, within CCNA_02223 at 2372.564 kb on - strand, within CCNA_02223 at 2372.606 kb on + strand, within CCNA_02223 at 2372.615 kb on + strand, within CCNA_02223 at 2372.636 kb on + strand, within CCNA_02223 at 2372.636 kb on + strand, within CCNA_02223 at 2372.644 kb on - strand, within CCNA_02223 at 2372.690 kb on + strand at 2372.735 kb on - strand at 2372.750 kb on + strand at 2372.750 kb on + strand at 2372.771 kb on - strand at 2372.780 kb on + strand at 2372.781 kb on + strand at 2372.786 kb on + strand at 2372.794 kb on - strand at 2372.820 kb on + strand at 2372.827 kb on - strand at 2372.833 kb on + strand at 2372.853 kb on - strand, within CCNA_02224 at 2372.991 kb on + strand, within CCNA_02224 at 2373.024 kb on - strand, within CCNA_02224 at 2373.097 kb on + strand, within CCNA_02224 at 2373.196 kb on + strand, within CCNA_02224 at 2373.199 kb on - strand, within CCNA_02224 at 2373.199 kb on - strand, within CCNA_02224 at 2373.202 kb on + strand, within CCNA_02224 at 2373.202 kb on + strand, within CCNA_02224 at 2373.210 kb on - strand, within CCNA_02224 at 2373.214 kb on + strand, within CCNA_02224 at 2373.224 kb on + strand, within CCNA_02224 at 2373.241 kb on + strand, within CCNA_02224 at 2373.241 kb on + strand, within CCNA_02224 at 2373.249 kb on - strand, within CCNA_02224 at 2373.249 kb on - strand, within CCNA_02224 at 2373.254 kb on - strand, within CCNA_02224 at 2373.272 kb on - strand, within CCNA_02224 at 2373.273 kb on - strand, within CCNA_02224 at 2373.284 kb on + strand, within CCNA_02224 at 2373.414 kb on + strand, within CCNA_02224 at 2373.414 kb on + strand, within CCNA_02224 at 2373.422 kb on - strand, within CCNA_02224 at 2373.422 kb on - strand, within CCNA_02224 at 2373.439 kb on - strand, within CCNA_02224 at 2373.450 kb on - strand, within CCNA_02224 at 2373.500 kb on + strand, within CCNA_02224 at 2373.500 kb on + strand, within CCNA_02224 at 2373.508 kb on - strand, within CCNA_02224 at 2373.508 kb on - strand, within CCNA_02224 at 2373.508 kb on - strand, within CCNA_02224 at 2373.508 kb on - strand, within CCNA_02224 at 2373.510 kb on - strand, within CCNA_02224 at 2373.539 kb on + strand, within CCNA_02224 at 2373.543 kb on + strand, within CCNA_02224 at 2373.575 kb on - strand, within CCNA_02224 at 2373.583 kb on + strand, within CCNA_02224 at 2373.583 kb on + strand, within CCNA_02224 at 2373.583 kb on + strand, within CCNA_02224 at 2373.591 kb on - strand at 2373.667 kb on - strand at 2373.671 kb on + strand at 2373.692 kb on + strand at 2373.694 kb on + strand at 2373.700 kb on - strand at 2373.700 kb on - strand at 2373.734 kb on - strand at 2373.734 kb on - strand at 2373.787 kb on + strand, within CCNA_02225 at 2373.818 kb on + strand, within CCNA_02225 at 2373.856 kb on - strand, within CCNA_02225 at 2373.944 kb on - strand, within CCNA_02225 at 2373.971 kb on + strand, within CCNA_02225 at 2373.995 kb on - strand, within CCNA_02225 at 2374.100 kb on - strand, within CCNA_02225 at 2374.122 kb on - strand, within CCNA_02225 at 2374.249 kb on + strand, within CCNA_02225 at 2374.257 kb on - strand, within CCNA_02225 at 2374.299 kb on + strand, within CCNA_02225 at 2374.307 kb on - strand, within CCNA_02225 at 2374.317 kb on + strand, within CCNA_02225 at 2374.324 kb on - strand, within CCNA_02225 at 2374.353 kb on + strand, within CCNA_02225 at 2374.458 kb on - strand, within CCNA_02225 at 2374.458 kb on - strand, within CCNA_02225 at 2374.458 kb on - strand, within CCNA_02225 at 2374.458 kb on - strand, within CCNA_02225 at 2374.499 kb on - strand, within CCNA_02225 at 2374.539 kb on - strand, within CCNA_02225 at 2374.558 kb on - strand, within CCNA_02225
Per-strain Table
Position Strand Gene LocusTag Fraction Putrescine (N) remove 2,371,755 + CCNA_02222 0.89 -1.0 2,371,763 - CCNA_02222 0.89 -1.0 2,371,827 - -0.4 2,371,838 - +0.2 2,371,840 - +0.1 2,371,915 + -2.4 2,371,937 + -3.3 2,372,003 - CCNA_02223 0.15 +0.8 2,372,009 + CCNA_02223 0.16 -0.3 2,372,020 + CCNA_02223 0.17 -0.4 2,372,028 - CCNA_02223 0.18 -0.0 2,372,051 - CCNA_02223 0.20 -0.1 2,372,063 + CCNA_02223 0.22 -2.1 2,372,071 - CCNA_02223 0.23 +1.2 2,372,078 + CCNA_02223 0.24 +0.4 2,372,117 - CCNA_02223 0.28 +0.0 2,372,123 + CCNA_02223 0.29 +1.6 2,372,177 + CCNA_02223 0.35 -1.3 2,372,177 + CCNA_02223 0.35 -0.9 2,372,210 - CCNA_02223 0.39 +2.7 2,372,211 - CCNA_02223 0.39 +2.2 2,372,225 + CCNA_02223 0.40 +0.1 2,372,276 - CCNA_02223 0.46 -0.5 2,372,276 - CCNA_02223 0.46 +0.3 2,372,276 - CCNA_02223 0.46 +0.7 2,372,276 - CCNA_02223 0.46 +0.1 2,372,276 - CCNA_02223 0.46 +1.1 2,372,276 - CCNA_02223 0.46 +0.6 2,372,281 - CCNA_02223 0.47 +0.9 2,372,281 - CCNA_02223 0.47 +0.8 2,372,296 - CCNA_02223 0.49 +1.0 2,372,390 + CCNA_02223 0.59 -0.8 2,372,390 + CCNA_02223 0.59 -2.0 2,372,405 - CCNA_02223 0.61 +0.3 2,372,407 + CCNA_02223 0.61 -0.1 2,372,414 + CCNA_02223 0.62 -2.0 2,372,414 + CCNA_02223 0.62 -1.9 2,372,422 - CCNA_02223 0.63 +0.5 2,372,422 - CCNA_02223 0.63 +1.0 2,372,422 - CCNA_02223 0.63 +1.5 2,372,422 - CCNA_02223 0.63 +2.2 2,372,472 - CCNA_02223 0.69 -0.3 2,372,501 + CCNA_02223 0.72 -1.4 2,372,502 - CCNA_02223 0.72 +0.0 2,372,559 - CCNA_02223 0.79 -0.3 2,372,564 - CCNA_02223 0.79 +0.4 2,372,606 + CCNA_02223 0.84 -1.1 2,372,615 + CCNA_02223 0.85 -2.7 2,372,636 + CCNA_02223 0.88 +0.8 2,372,636 + CCNA_02223 0.88 -3.2 2,372,644 - CCNA_02223 0.89 +0.3 2,372,690 + -1.0 2,372,735 - +0.3 2,372,750 + -1.3 2,372,750 + -1.1 2,372,771 - +0.2 2,372,780 + -1.1 2,372,781 + -2.7 2,372,786 + -1.5 2,372,794 - -0.6 2,372,820 + -2.9 2,372,827 - -5.1 2,372,833 + -1.7 2,372,853 - CCNA_02224 0.12 -3.5 2,372,991 + CCNA_02224 0.27 -1.5 2,373,024 - CCNA_02224 0.30 -2.5 2,373,097 + CCNA_02224 0.38 -3.5 2,373,196 + CCNA_02224 0.48 -1.3 2,373,199 - CCNA_02224 0.49 -2.7 2,373,199 - CCNA_02224 0.49 -3.1 2,373,202 + CCNA_02224 0.49 -0.6 2,373,202 + CCNA_02224 0.49 -1.6 2,373,210 - CCNA_02224 0.50 -2.4 2,373,214 + CCNA_02224 0.50 -2.3 2,373,224 + CCNA_02224 0.51 -3.4 2,373,241 + CCNA_02224 0.53 -4.9 2,373,241 + CCNA_02224 0.53 -2.3 2,373,249 - CCNA_02224 0.54 -3.8 2,373,249 - CCNA_02224 0.54 -1.9 2,373,254 - CCNA_02224 0.54 -3.1 2,373,272 - CCNA_02224 0.56 -2.1 2,373,273 - CCNA_02224 0.57 -2.0 2,373,284 + CCNA_02224 0.58 -2.4 2,373,414 + CCNA_02224 0.71 -1.7 2,373,414 + CCNA_02224 0.71 -2.2 2,373,422 - CCNA_02224 0.72 -2.8 2,373,422 - CCNA_02224 0.72 -0.7 2,373,439 - CCNA_02224 0.74 -0.6 2,373,450 - CCNA_02224 0.75 -3.4 2,373,500 + CCNA_02224 0.81 -0.8 2,373,500 + CCNA_02224 0.81 -2.0 2,373,508 - CCNA_02224 0.81 -2.9 2,373,508 - CCNA_02224 0.81 -1.9 2,373,508 - CCNA_02224 0.81 -2.9 2,373,508 - CCNA_02224 0.81 -1.1 2,373,510 - CCNA_02224 0.82 -4.8 2,373,539 + CCNA_02224 0.85 -3.4 2,373,543 + CCNA_02224 0.85 -1.9 2,373,575 - CCNA_02224 0.88 -4.2 2,373,583 + CCNA_02224 0.89 -0.2 2,373,583 + CCNA_02224 0.89 -1.4 2,373,583 + CCNA_02224 0.89 -1.6 2,373,591 - -1.9 2,373,667 - -4.2 2,373,671 + -3.0 2,373,692 + -0.9 2,373,694 + -3.5 2,373,700 - +2.0 2,373,700 - +1.3 2,373,734 - +3.4 2,373,734 - +2.9 2,373,787 + CCNA_02225 0.11 +1.4 2,373,818 + CCNA_02225 0.14 -1.1 2,373,856 - CCNA_02225 0.18 +3.5 2,373,944 - CCNA_02225 0.27 +3.3 2,373,971 + CCNA_02225 0.30 -0.0 2,373,995 - CCNA_02225 0.32 +4.2 2,374,100 - CCNA_02225 0.43 +3.8 2,374,122 - CCNA_02225 0.45 +3.8 2,374,249 + CCNA_02225 0.58 -0.6 2,374,257 - CCNA_02225 0.59 +1.2 2,374,299 + CCNA_02225 0.63 +2.0 2,374,307 - CCNA_02225 0.64 +4.0 2,374,317 + CCNA_02225 0.65 -1.8 2,374,324 - CCNA_02225 0.66 +3.5 2,374,353 + CCNA_02225 0.69 -1.1 2,374,458 - CCNA_02225 0.80 -2.0 2,374,458 - CCNA_02225 0.80 -2.4 2,374,458 - CCNA_02225 0.80 +4.0 2,374,458 - CCNA_02225 0.80 -3.0 2,374,499 - CCNA_02225 0.84 +3.1 2,374,539 - CCNA_02225 0.88 +0.9 2,374,558 - CCNA_02225 0.90 -1.7
Or see this region's nucleotide sequence