Experiment: R2A with Chloramphenicol 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS22635 and CA265_RS22640 are separated by 237 nucleotides CA265_RS22640 and CA265_RS22645 are separated by 146 nucleotides
CA265_RS22635: CA265_RS22635 - phosphoserine phosphatase (EC 3.1.3.3) (from data) , at 5,542,233 to 5,543,534
_RS22635
CA265_RS22640: CA265_RS22640 - chloride channel protein, at 5,543,772 to 5,545,061
_RS22640
CA265_RS22645: CA265_RS22645 - tryptophan 2,3-dioxygenase, at 5,545,208 to 5,546,173
_RS22645
Position (kb)
5543
5544
5545
5546 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 5543.079 kb on - strand, within CA265_RS22635 at 5543.128 kb on + strand, within CA265_RS22635 at 5543.508 kb on - strand at 5543.580 kb on - strand at 5543.681 kb on + strand at 5543.681 kb on + strand at 5543.742 kb on + strand at 5543.743 kb on - strand at 5543.863 kb on + strand at 5543.919 kb on + strand, within CA265_RS22640 at 5543.928 kb on - strand, within CA265_RS22640 at 5543.933 kb on + strand, within CA265_RS22640 at 5544.114 kb on + strand, within CA265_RS22640 at 5544.115 kb on - strand, within CA265_RS22640 at 5544.131 kb on + strand, within CA265_RS22640 at 5544.132 kb on - strand, within CA265_RS22640 at 5544.232 kb on - strand, within CA265_RS22640 at 5544.334 kb on - strand, within CA265_RS22640 at 5544.337 kb on - strand, within CA265_RS22640 at 5544.358 kb on - strand, within CA265_RS22640 at 5544.360 kb on + strand, within CA265_RS22640 at 5544.435 kb on - strand, within CA265_RS22640 at 5544.437 kb on - strand at 5544.624 kb on - strand, within CA265_RS22640 at 5544.690 kb on + strand, within CA265_RS22640 at 5544.702 kb on - strand, within CA265_RS22640 at 5544.910 kb on - strand, within CA265_RS22640 at 5544.973 kb on - strand at 5545.010 kb on + strand at 5545.158 kb on - strand at 5545.161 kb on + strand at 5545.162 kb on - strand at 5545.197 kb on - strand at 5545.228 kb on - strand at 5545.228 kb on - strand at 5545.239 kb on + strand at 5545.248 kb on + strand at 5545.248 kb on + strand at 5545.260 kb on - strand at 5545.306 kb on - strand, within CA265_RS22645 at 5545.308 kb on - strand, within CA265_RS22645 at 5545.326 kb on - strand, within CA265_RS22645 at 5545.334 kb on + strand, within CA265_RS22645 at 5545.335 kb on - strand, within CA265_RS22645 at 5545.409 kb on + strand, within CA265_RS22645 at 5545.410 kb on - strand, within CA265_RS22645 at 5545.436 kb on - strand, within CA265_RS22645 at 5545.539 kb on + strand, within CA265_RS22645 at 5545.609 kb on - strand, within CA265_RS22645 at 5545.672 kb on + strand, within CA265_RS22645 at 5545.707 kb on + strand, within CA265_RS22645 at 5545.707 kb on + strand, within CA265_RS22645 at 5545.722 kb on + strand, within CA265_RS22645 at 5545.722 kb on + strand, within CA265_RS22645 at 5545.723 kb on - strand, within CA265_RS22645 at 5545.723 kb on - strand, within CA265_RS22645 at 5545.742 kb on - strand, within CA265_RS22645 at 5545.877 kb on + strand, within CA265_RS22645 at 5545.877 kb on + strand, within CA265_RS22645 at 5545.878 kb on - strand, within CA265_RS22645 at 5545.946 kb on + strand, within CA265_RS22645 at 5545.951 kb on - strand, within CA265_RS22645 at 5545.990 kb on - strand, within CA265_RS22645 at 5546.017 kb on + strand, within CA265_RS22645 at 5546.017 kb on + strand, within CA265_RS22645
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloramphenicol 0.005 mg/ml remove 5,543,079 - CA265_RS22635 0.65 +0.2 5,543,128 + CA265_RS22635 0.69 -0.1 5,543,508 - +0.7 5,543,580 - +0.7 5,543,681 + +0.5 5,543,681 + -0.3 5,543,742 + +0.0 5,543,743 - -2.1 5,543,863 + -1.2 5,543,919 + CA265_RS22640 0.11 +0.2 5,543,928 - CA265_RS22640 0.12 -0.6 5,543,933 + CA265_RS22640 0.12 -0.2 5,544,114 + CA265_RS22640 0.27 -0.3 5,544,115 - CA265_RS22640 0.27 -2.2 5,544,131 + CA265_RS22640 0.28 -0.7 5,544,132 - CA265_RS22640 0.28 +0.4 5,544,232 - CA265_RS22640 0.36 -1.3 5,544,334 - CA265_RS22640 0.44 -1.3 5,544,337 - CA265_RS22640 0.44 -1.1 5,544,358 - CA265_RS22640 0.45 -1.0 5,544,360 + CA265_RS22640 0.46 -1.0 5,544,435 - CA265_RS22640 0.51 +0.3 5,544,437 - +0.9 5,544,624 - CA265_RS22640 0.66 -0.9 5,544,690 + CA265_RS22640 0.71 -1.9 5,544,702 - CA265_RS22640 0.72 -2.8 5,544,910 - CA265_RS22640 0.88 +1.4 5,544,973 - +0.0 5,545,010 + -0.4 5,545,158 - +0.6 5,545,161 + +0.6 5,545,162 - -0.8 5,545,197 - -0.1 5,545,228 - +0.1 5,545,228 - +1.0 5,545,239 + +1.4 5,545,248 + +0.3 5,545,248 + -0.7 5,545,260 - +0.5 5,545,306 - CA265_RS22645 0.10 +0.8 5,545,308 - CA265_RS22645 0.10 +0.4 5,545,326 - CA265_RS22645 0.12 -0.1 5,545,334 + CA265_RS22645 0.13 +0.5 5,545,335 - CA265_RS22645 0.13 -0.4 5,545,409 + CA265_RS22645 0.21 -1.0 5,545,410 - CA265_RS22645 0.21 +0.3 5,545,436 - CA265_RS22645 0.24 -0.1 5,545,539 + CA265_RS22645 0.34 -0.7 5,545,609 - CA265_RS22645 0.42 +0.7 5,545,672 + CA265_RS22645 0.48 +0.1 5,545,707 + CA265_RS22645 0.52 -1.1 5,545,707 + CA265_RS22645 0.52 -1.5 5,545,722 + CA265_RS22645 0.53 -0.6 5,545,722 + CA265_RS22645 0.53 -0.8 5,545,723 - CA265_RS22645 0.53 -0.1 5,545,723 - CA265_RS22645 0.53 -0.3 5,545,742 - CA265_RS22645 0.55 -0.1 5,545,877 + CA265_RS22645 0.69 -0.1 5,545,877 + CA265_RS22645 0.69 +0.0 5,545,878 - CA265_RS22645 0.69 +0.6 5,545,946 + CA265_RS22645 0.76 -1.7 5,545,951 - CA265_RS22645 0.77 -0.5 5,545,990 - CA265_RS22645 0.81 +0.4 5,546,017 + CA265_RS22645 0.84 +0.9 5,546,017 + CA265_RS22645 0.84 +0.6
Or see this region's nucleotide sequence