Experiment: R2A with Chloramphenicol 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS22005 and CA265_RS22010 are separated by 112 nucleotides CA265_RS22010 and CA265_RS22015 are separated by 165 nucleotides
CA265_RS22005: CA265_RS22005 - S9 family peptidase, at 5,413,158 to 5,415,284
_RS22005
CA265_RS22010: CA265_RS22010 - hypothetical protein, at 5,415,397 to 5,415,648
_RS22010
CA265_RS22015: CA265_RS22015 - UDP-2,3-diacylglucosamine hydrolase, at 5,415,814 to 5,416,563
_RS22015
Position (kb)
5415
5416 Strain fitness (log2 ratio)
-2
-1
0
1 at 5414.400 kb on + strand, within CA265_RS22005 at 5414.427 kb on + strand, within CA265_RS22005 at 5414.428 kb on - strand, within CA265_RS22005 at 5414.440 kb on + strand, within CA265_RS22005 at 5414.441 kb on - strand, within CA265_RS22005 at 5414.519 kb on - strand, within CA265_RS22005 at 5414.578 kb on - strand, within CA265_RS22005 at 5414.660 kb on - strand, within CA265_RS22005 at 5414.747 kb on + strand, within CA265_RS22005 at 5414.750 kb on - strand, within CA265_RS22005 at 5414.750 kb on - strand, within CA265_RS22005 at 5414.750 kb on - strand, within CA265_RS22005 at 5414.916 kb on - strand, within CA265_RS22005 at 5414.992 kb on + strand, within CA265_RS22005 at 5414.993 kb on - strand, within CA265_RS22005 at 5415.107 kb on + strand at 5415.108 kb on - strand at 5415.154 kb on + strand at 5415.158 kb on + strand at 5415.168 kb on - strand at 5415.190 kb on + strand at 5415.259 kb on - strand at 5415.312 kb on - strand at 5415.312 kb on - strand at 5415.331 kb on + strand at 5415.334 kb on + strand at 5415.337 kb on + strand at 5415.338 kb on - strand at 5415.389 kb on - strand at 5415.450 kb on + strand, within CA265_RS22010 at 5415.566 kb on - strand, within CA265_RS22010 at 5415.586 kb on + strand, within CA265_RS22010 at 5415.748 kb on + strand at 5415.757 kb on - strand at 5415.777 kb on + strand at 5416.543 kb on + strand at 5416.546 kb on + strand at 5416.546 kb on + strand at 5416.546 kb on + strand at 5416.547 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloramphenicol 0.005 mg/ml remove 5,414,400 + CA265_RS22005 0.58 +0.3 5,414,427 + CA265_RS22005 0.60 +0.6 5,414,428 - CA265_RS22005 0.60 +0.4 5,414,440 + CA265_RS22005 0.60 -0.4 5,414,441 - CA265_RS22005 0.60 -0.4 5,414,519 - CA265_RS22005 0.64 +0.0 5,414,578 - CA265_RS22005 0.67 +0.2 5,414,660 - CA265_RS22005 0.71 -0.2 5,414,747 + CA265_RS22005 0.75 +0.5 5,414,750 - CA265_RS22005 0.75 -0.6 5,414,750 - CA265_RS22005 0.75 +0.9 5,414,750 - CA265_RS22005 0.75 -0.5 5,414,916 - CA265_RS22005 0.83 +0.0 5,414,992 + CA265_RS22005 0.86 -0.0 5,414,993 - CA265_RS22005 0.86 -1.3 5,415,107 + -0.7 5,415,108 - -0.1 5,415,154 + +0.4 5,415,158 + -1.9 5,415,168 - +0.2 5,415,190 + -0.7 5,415,259 - +1.6 5,415,312 - -0.4 5,415,312 - -0.9 5,415,331 + +0.9 5,415,334 + +0.2 5,415,337 + -0.0 5,415,338 - +0.3 5,415,389 - -0.9 5,415,450 + CA265_RS22010 0.21 +0.6 5,415,566 - CA265_RS22010 0.67 +1.3 5,415,586 + CA265_RS22010 0.75 +0.1 5,415,748 + +1.1 5,415,757 - -0.3 5,415,777 + -0.1 5,416,543 + +1.2 5,416,546 + +0.4 5,416,546 + -0.0 5,416,546 + -0.4 5,416,547 - -0.1
Or see this region's nucleotide sequence