Experiment: R2A with Chloramphenicol 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS21185 and CA265_RS21190 are separated by 100 nucleotides CA265_RS21190 and CA265_RS21195 are separated by 97 nucleotides CA265_RS21195 and CA265_RS21200 are separated by 121 nucleotides
CA265_RS21185: CA265_RS21185 - L-aspartate oxidase, at 5,215,757 to 5,217,343
_RS21185
CA265_RS21190: CA265_RS21190 - gamma carbonic anhydrase family protein, at 5,217,444 to 5,217,956
_RS21190
CA265_RS21195: CA265_RS21195 - hypothetical protein, at 5,218,054 to 5,219,697
_RS21195
CA265_RS21200: CA265_RS21200 - tRNA uridine-5-carboxymethylaminomethyl(34) synthesis enzyme MnmG, at 5,219,819 to 5,221,681
_RS21200
Position (kb)
5218
5219
5220 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5217.055 kb on - strand, within CA265_RS21185 at 5217.061 kb on + strand, within CA265_RS21185 at 5217.062 kb on - strand, within CA265_RS21185 at 5217.096 kb on - strand, within CA265_RS21185 at 5217.099 kb on + strand, within CA265_RS21185 at 5217.109 kb on - strand, within CA265_RS21185 at 5217.291 kb on - strand at 5217.347 kb on - strand at 5217.378 kb on + strand at 5217.383 kb on - strand at 5217.397 kb on - strand at 5217.437 kb on + strand at 5217.454 kb on + strand at 5217.474 kb on + strand at 5217.500 kb on + strand, within CA265_RS21190 at 5217.533 kb on - strand, within CA265_RS21190 at 5217.533 kb on - strand, within CA265_RS21190 at 5217.587 kb on - strand at 5217.596 kb on + strand, within CA265_RS21190 at 5217.662 kb on + strand, within CA265_RS21190 at 5217.662 kb on + strand, within CA265_RS21190 at 5217.662 kb on + strand, within CA265_RS21190 at 5217.695 kb on + strand, within CA265_RS21190 at 5217.703 kb on + strand, within CA265_RS21190 at 5217.703 kb on + strand, within CA265_RS21190 at 5217.707 kb on - strand, within CA265_RS21190 at 5217.715 kb on - strand, within CA265_RS21190 at 5217.717 kb on - strand, within CA265_RS21190 at 5217.783 kb on + strand, within CA265_RS21190 at 5217.788 kb on + strand, within CA265_RS21190 at 5217.789 kb on - strand, within CA265_RS21190 at 5217.841 kb on - strand, within CA265_RS21190 at 5217.844 kb on + strand, within CA265_RS21190 at 5217.845 kb on - strand, within CA265_RS21190 at 5217.901 kb on + strand, within CA265_RS21190 at 5217.924 kb on + strand at 5217.933 kb on + strand at 5217.933 kb on + strand at 5217.955 kb on - strand at 5218.008 kb on + strand at 5218.008 kb on + strand at 5218.121 kb on - strand at 5218.165 kb on + strand at 5218.194 kb on + strand at 5218.210 kb on + strand at 5218.221 kb on + strand, within CA265_RS21195 at 5218.223 kb on + strand, within CA265_RS21195 at 5218.224 kb on - strand, within CA265_RS21195 at 5218.358 kb on - strand, within CA265_RS21195 at 5218.371 kb on - strand, within CA265_RS21195 at 5218.371 kb on - strand, within CA265_RS21195 at 5218.406 kb on - strand, within CA265_RS21195 at 5218.406 kb on - strand, within CA265_RS21195 at 5218.490 kb on + strand, within CA265_RS21195 at 5218.503 kb on - strand, within CA265_RS21195 at 5218.516 kb on + strand, within CA265_RS21195 at 5218.529 kb on - strand, within CA265_RS21195 at 5218.529 kb on - strand, within CA265_RS21195 at 5218.684 kb on - strand, within CA265_RS21195 at 5218.684 kb on - strand, within CA265_RS21195 at 5218.685 kb on + strand, within CA265_RS21195 at 5218.686 kb on - strand, within CA265_RS21195 at 5218.745 kb on - strand, within CA265_RS21195 at 5218.780 kb on + strand, within CA265_RS21195 at 5218.832 kb on - strand, within CA265_RS21195 at 5218.838 kb on - strand, within CA265_RS21195 at 5218.875 kb on + strand, within CA265_RS21195 at 5218.876 kb on - strand, within CA265_RS21195 at 5218.889 kb on - strand, within CA265_RS21195 at 5218.900 kb on + strand, within CA265_RS21195 at 5218.915 kb on - strand, within CA265_RS21195 at 5218.931 kb on - strand, within CA265_RS21195 at 5218.964 kb on - strand, within CA265_RS21195 at 5218.976 kb on - strand, within CA265_RS21195 at 5218.996 kb on + strand, within CA265_RS21195 at 5218.997 kb on - strand, within CA265_RS21195 at 5219.000 kb on - strand, within CA265_RS21195 at 5219.014 kb on + strand, within CA265_RS21195 at 5219.087 kb on - strand, within CA265_RS21195 at 5219.087 kb on - strand, within CA265_RS21195 at 5219.087 kb on - strand, within CA265_RS21195 at 5219.107 kb on + strand, within CA265_RS21195 at 5219.108 kb on - strand, within CA265_RS21195 at 5219.123 kb on + strand, within CA265_RS21195 at 5219.178 kb on + strand, within CA265_RS21195 at 5219.178 kb on + strand, within CA265_RS21195 at 5219.181 kb on + strand, within CA265_RS21195 at 5219.182 kb on - strand, within CA265_RS21195 at 5219.244 kb on + strand, within CA265_RS21195 at 5219.260 kb on + strand, within CA265_RS21195 at 5219.261 kb on - strand, within CA265_RS21195 at 5219.365 kb on - strand, within CA265_RS21195 at 5219.367 kb on - strand, within CA265_RS21195 at 5219.388 kb on + strand, within CA265_RS21195 at 5219.389 kb on - strand, within CA265_RS21195 at 5219.495 kb on + strand, within CA265_RS21195 at 5219.513 kb on - strand, within CA265_RS21195 at 5219.638 kb on + strand at 5219.638 kb on + strand at 5219.639 kb on - strand at 5219.639 kb on - strand at 5219.639 kb on - strand at 5219.767 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloramphenicol 0.005 mg/ml remove 5,217,055 - CA265_RS21185 0.82 -2.6 5,217,061 + CA265_RS21185 0.82 -1.4 5,217,062 - CA265_RS21185 0.82 -2.6 5,217,096 - CA265_RS21185 0.84 -2.2 5,217,099 + CA265_RS21185 0.85 -0.6 5,217,109 - CA265_RS21185 0.85 -0.6 5,217,291 - -2.0 5,217,347 - +0.5 5,217,378 + +0.0 5,217,383 - +0.9 5,217,397 - -1.0 5,217,437 + +0.4 5,217,454 + -0.2 5,217,474 + -0.6 5,217,500 + CA265_RS21190 0.11 -0.9 5,217,533 - CA265_RS21190 0.17 +0.2 5,217,533 - CA265_RS21190 0.17 -0.4 5,217,587 - +1.4 5,217,596 + CA265_RS21190 0.30 +1.1 5,217,662 + CA265_RS21190 0.42 +0.3 5,217,662 + CA265_RS21190 0.42 +0.7 5,217,662 + CA265_RS21190 0.42 +0.0 5,217,695 + CA265_RS21190 0.49 -0.4 5,217,703 + CA265_RS21190 0.50 +0.3 5,217,703 + CA265_RS21190 0.50 -0.2 5,217,707 - CA265_RS21190 0.51 -0.5 5,217,715 - CA265_RS21190 0.53 +0.6 5,217,717 - CA265_RS21190 0.53 +0.7 5,217,783 + CA265_RS21190 0.66 -0.5 5,217,788 + CA265_RS21190 0.67 -0.2 5,217,789 - CA265_RS21190 0.67 +1.7 5,217,841 - CA265_RS21190 0.77 -0.1 5,217,844 + CA265_RS21190 0.78 +0.6 5,217,845 - CA265_RS21190 0.78 +1.4 5,217,901 + CA265_RS21190 0.89 -1.3 5,217,924 + +0.0 5,217,933 + +0.9 5,217,933 + -0.0 5,217,955 - -0.1 5,218,008 + +1.1 5,218,008 + -0.8 5,218,121 - -1.0 5,218,165 + -1.9 5,218,194 + -2.0 5,218,210 + +1.9 5,218,221 + CA265_RS21195 0.10 -0.1 5,218,223 + CA265_RS21195 0.10 -2.4 5,218,224 - CA265_RS21195 0.10 +0.0 5,218,358 - CA265_RS21195 0.18 +0.4 5,218,371 - CA265_RS21195 0.19 -1.3 5,218,371 - CA265_RS21195 0.19 -0.9 5,218,406 - CA265_RS21195 0.21 -2.1 5,218,406 - CA265_RS21195 0.21 -0.5 5,218,490 + CA265_RS21195 0.27 -0.1 5,218,503 - CA265_RS21195 0.27 -0.6 5,218,516 + CA265_RS21195 0.28 -1.1 5,218,529 - CA265_RS21195 0.29 -1.3 5,218,529 - CA265_RS21195 0.29 -0.4 5,218,684 - CA265_RS21195 0.38 -0.5 5,218,684 - CA265_RS21195 0.38 -0.7 5,218,685 + CA265_RS21195 0.38 +0.5 5,218,686 - CA265_RS21195 0.38 +0.4 5,218,745 - CA265_RS21195 0.42 -0.3 5,218,780 + CA265_RS21195 0.44 +0.0 5,218,832 - CA265_RS21195 0.47 +0.3 5,218,838 - CA265_RS21195 0.48 -0.2 5,218,875 + CA265_RS21195 0.50 -0.3 5,218,876 - CA265_RS21195 0.50 -0.3 5,218,889 - CA265_RS21195 0.51 -1.7 5,218,900 + CA265_RS21195 0.51 -0.7 5,218,915 - CA265_RS21195 0.52 -0.3 5,218,931 - CA265_RS21195 0.53 -2.9 5,218,964 - CA265_RS21195 0.55 -0.1 5,218,976 - CA265_RS21195 0.56 +0.3 5,218,996 + CA265_RS21195 0.57 -0.4 5,218,997 - CA265_RS21195 0.57 +0.2 5,219,000 - CA265_RS21195 0.58 -0.4 5,219,014 + CA265_RS21195 0.58 -0.7 5,219,087 - CA265_RS21195 0.63 +1.1 5,219,087 - CA265_RS21195 0.63 +0.7 5,219,087 - CA265_RS21195 0.63 -2.0 5,219,107 + CA265_RS21195 0.64 -1.0 5,219,108 - CA265_RS21195 0.64 +0.9 5,219,123 + CA265_RS21195 0.65 -1.4 5,219,178 + CA265_RS21195 0.68 -0.2 5,219,178 + CA265_RS21195 0.68 +0.1 5,219,181 + CA265_RS21195 0.69 -0.2 5,219,182 - CA265_RS21195 0.69 -0.2 5,219,244 + CA265_RS21195 0.72 -0.8 5,219,260 + CA265_RS21195 0.73 -0.4 5,219,261 - CA265_RS21195 0.73 -0.1 5,219,365 - CA265_RS21195 0.80 -0.7 5,219,367 - CA265_RS21195 0.80 -1.0 5,219,388 + CA265_RS21195 0.81 -0.1 5,219,389 - CA265_RS21195 0.81 -1.0 5,219,495 + CA265_RS21195 0.88 -0.1 5,219,513 - CA265_RS21195 0.89 -1.1 5,219,638 + +1.2 5,219,638 + -1.3 5,219,639 - -1.1 5,219,639 - -0.1 5,219,639 - -0.0 5,219,767 - +0.3
Or see this region's nucleotide sequence