Experiment: R2A with Chloramphenicol 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS13285 and CA265_RS13290 are separated by 294 nucleotides CA265_RS13290 and CA265_RS13295 are separated by 200 nucleotides CA265_RS13295 and CA265_RS13300 are separated by 3 nucleotides
CA265_RS13285: CA265_RS13285 - magnesium transporter, at 3,299,305 to 3,300,681
_RS13285
CA265_RS13290: CA265_RS13290 - tRNA-Arg, at 3,300,976 to 3,301,050
_RS13290
CA265_RS13295: CA265_RS13295 - hypothetical protein, at 3,301,251 to 3,303,740
_RS13295
CA265_RS13300: CA265_RS13300 - hypothetical protein, at 3,303,744 to 3,304,880
_RS13300
Position (kb)
3301
3302
3303
3304 Strain fitness (log2 ratio)
-1
0
1
2 at 3300.268 kb on - strand, within CA265_RS13285 at 3300.294 kb on + strand, within CA265_RS13285 at 3300.307 kb on - strand, within CA265_RS13285 at 3300.308 kb on + strand, within CA265_RS13285 at 3300.309 kb on - strand, within CA265_RS13285 at 3300.554 kb on + strand at 3300.555 kb on - strand at 3300.583 kb on + strand at 3300.583 kb on + strand at 3300.594 kb on + strand at 3300.620 kb on - strand at 3300.708 kb on + strand at 3300.708 kb on + strand at 3300.851 kb on - strand at 3300.874 kb on - strand at 3300.909 kb on + strand at 3300.909 kb on + strand at 3300.917 kb on - strand at 3300.961 kb on + strand at 3301.118 kb on + strand at 3301.124 kb on + strand at 3301.124 kb on + strand at 3301.124 kb on + strand at 3301.125 kb on - strand at 3301.229 kb on - strand at 3301.229 kb on - strand at 3301.229 kb on - strand at 3301.252 kb on + strand at 3301.253 kb on - strand at 3301.372 kb on - strand at 3301.382 kb on - strand at 3301.385 kb on + strand at 3301.385 kb on + strand at 3301.386 kb on - strand at 3301.386 kb on - strand at 3301.406 kb on + strand at 3301.474 kb on - strand at 3301.534 kb on + strand, within CA265_RS13295 at 3301.552 kb on + strand, within CA265_RS13295 at 3301.617 kb on - strand, within CA265_RS13295 at 3301.678 kb on + strand, within CA265_RS13295 at 3301.678 kb on + strand, within CA265_RS13295 at 3301.784 kb on - strand, within CA265_RS13295 at 3301.784 kb on - strand, within CA265_RS13295 at 3301.825 kb on - strand, within CA265_RS13295 at 3301.862 kb on + strand, within CA265_RS13295 at 3301.862 kb on + strand, within CA265_RS13295 at 3301.918 kb on - strand, within CA265_RS13295 at 3301.939 kb on + strand, within CA265_RS13295 at 3301.940 kb on - strand, within CA265_RS13295 at 3301.941 kb on + strand, within CA265_RS13295 at 3302.012 kb on - strand, within CA265_RS13295 at 3302.090 kb on + strand, within CA265_RS13295 at 3302.135 kb on - strand, within CA265_RS13295 at 3302.135 kb on - strand, within CA265_RS13295 at 3302.157 kb on + strand, within CA265_RS13295 at 3302.194 kb on + strand, within CA265_RS13295 at 3302.227 kb on + strand, within CA265_RS13295 at 3302.289 kb on - strand, within CA265_RS13295 at 3302.301 kb on + strand, within CA265_RS13295 at 3302.302 kb on - strand, within CA265_RS13295 at 3302.304 kb on - strand, within CA265_RS13295 at 3302.359 kb on + strand, within CA265_RS13295 at 3302.364 kb on + strand, within CA265_RS13295 at 3302.377 kb on + strand, within CA265_RS13295 at 3302.377 kb on + strand, within CA265_RS13295 at 3302.377 kb on + strand, within CA265_RS13295 at 3302.378 kb on - strand, within CA265_RS13295 at 3302.436 kb on + strand, within CA265_RS13295 at 3302.436 kb on + strand, within CA265_RS13295 at 3302.436 kb on + strand, within CA265_RS13295 at 3302.437 kb on - strand, within CA265_RS13295 at 3302.452 kb on + strand, within CA265_RS13295 at 3302.453 kb on - strand, within CA265_RS13295 at 3302.456 kb on + strand, within CA265_RS13295 at 3302.467 kb on - strand, within CA265_RS13295 at 3302.537 kb on + strand, within CA265_RS13295 at 3302.545 kb on + strand, within CA265_RS13295 at 3302.545 kb on + strand, within CA265_RS13295 at 3302.549 kb on + strand, within CA265_RS13295 at 3302.550 kb on - strand, within CA265_RS13295 at 3302.640 kb on - strand, within CA265_RS13295 at 3302.642 kb on + strand, within CA265_RS13295 at 3302.654 kb on + strand, within CA265_RS13295 at 3302.669 kb on + strand, within CA265_RS13295 at 3302.670 kb on - strand, within CA265_RS13295 at 3302.685 kb on + strand, within CA265_RS13295 at 3302.722 kb on - strand, within CA265_RS13295 at 3302.750 kb on + strand, within CA265_RS13295 at 3302.750 kb on + strand, within CA265_RS13295 at 3302.783 kb on + strand, within CA265_RS13295 at 3302.867 kb on - strand, within CA265_RS13295 at 3302.876 kb on + strand, within CA265_RS13295 at 3302.876 kb on + strand, within CA265_RS13295 at 3302.877 kb on - strand, within CA265_RS13295 at 3302.877 kb on - strand, within CA265_RS13295 at 3302.877 kb on - strand, within CA265_RS13295 at 3302.936 kb on - strand, within CA265_RS13295 at 3303.015 kb on - strand, within CA265_RS13295 at 3303.099 kb on - strand, within CA265_RS13295 at 3303.114 kb on + strand, within CA265_RS13295 at 3303.139 kb on + strand, within CA265_RS13295 at 3303.139 kb on + strand, within CA265_RS13295 at 3303.139 kb on + strand, within CA265_RS13295 at 3303.144 kb on + strand, within CA265_RS13295 at 3303.264 kb on + strand, within CA265_RS13295 at 3303.300 kb on + strand, within CA265_RS13295 at 3303.302 kb on + strand, within CA265_RS13295 at 3303.303 kb on - strand, within CA265_RS13295 at 3303.303 kb on - strand, within CA265_RS13295 at 3303.303 kb on - strand, within CA265_RS13295 at 3303.366 kb on + strand, within CA265_RS13295 at 3303.407 kb on + strand, within CA265_RS13295 at 3303.408 kb on - strand, within CA265_RS13295 at 3303.411 kb on - strand, within CA265_RS13295 at 3303.441 kb on - strand, within CA265_RS13295 at 3303.443 kb on - strand, within CA265_RS13295 at 3303.473 kb on + strand, within CA265_RS13295 at 3303.532 kb on - strand at 3303.533 kb on + strand at 3303.600 kb on + strand at 3303.601 kb on - strand at 3303.644 kb on + strand at 3303.677 kb on + strand at 3303.677 kb on + strand at 3303.692 kb on + strand at 3303.740 kb on + strand at 3303.761 kb on + strand at 3303.761 kb on + strand at 3303.762 kb on - strand at 3303.814 kb on + strand at 3303.853 kb on - strand at 3303.853 kb on - strand at 3303.911 kb on - strand, within CA265_RS13300 at 3303.972 kb on + strand, within CA265_RS13300 at 3304.024 kb on + strand, within CA265_RS13300 at 3304.024 kb on + strand, within CA265_RS13300 at 3304.024 kb on + strand, within CA265_RS13300 at 3304.025 kb on - strand, within CA265_RS13300 at 3304.025 kb on - strand, within CA265_RS13300 at 3304.025 kb on - strand, within CA265_RS13300 at 3304.025 kb on - strand, within CA265_RS13300 at 3304.025 kb on - strand, within CA265_RS13300 at 3304.029 kb on - strand, within CA265_RS13300 at 3304.038 kb on + strand, within CA265_RS13300 at 3304.038 kb on + strand, within CA265_RS13300 at 3304.038 kb on + strand, within CA265_RS13300 at 3304.039 kb on - strand, within CA265_RS13300 at 3304.039 kb on - strand, within CA265_RS13300 at 3304.039 kb on - strand, within CA265_RS13300 at 3304.043 kb on - strand, within CA265_RS13300 at 3304.043 kb on - strand, within CA265_RS13300 at 3304.043 kb on - strand, within CA265_RS13300 at 3304.057 kb on - strand, within CA265_RS13300 at 3304.057 kb on - strand, within CA265_RS13300 at 3304.182 kb on + strand, within CA265_RS13300 at 3304.228 kb on - strand, within CA265_RS13300 at 3304.300 kb on + strand, within CA265_RS13300 at 3304.498 kb on + strand, within CA265_RS13300 at 3304.498 kb on + strand, within CA265_RS13300 at 3304.499 kb on - strand, within CA265_RS13300 at 3304.499 kb on - strand, within CA265_RS13300 at 3304.499 kb on - strand, within CA265_RS13300 at 3304.499 kb on - strand, within CA265_RS13300 at 3304.499 kb on - strand, within CA265_RS13300 at 3304.502 kb on - strand, within CA265_RS13300 at 3304.529 kb on + strand, within CA265_RS13300 at 3304.530 kb on - strand, within CA265_RS13300 at 3304.568 kb on - strand, within CA265_RS13300 at 3304.575 kb on - strand, within CA265_RS13300 at 3304.605 kb on - strand, within CA265_RS13300 at 3304.608 kb on - strand, within CA265_RS13300 at 3304.651 kb on - strand, within CA265_RS13300 at 3304.693 kb on - strand, within CA265_RS13300 at 3304.693 kb on - strand, within CA265_RS13300 at 3304.709 kb on + strand, within CA265_RS13300 at 3304.709 kb on + strand, within CA265_RS13300
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloramphenicol 0.005 mg/ml remove 3,300,268 - CA265_RS13285 0.70 -1.5 3,300,294 + CA265_RS13285 0.72 -0.4 3,300,307 - CA265_RS13285 0.73 +0.1 3,300,308 + CA265_RS13285 0.73 +0.2 3,300,309 - CA265_RS13285 0.73 +1.9 3,300,554 + +0.3 3,300,555 - +0.7 3,300,583 + +0.2 3,300,583 + +0.2 3,300,594 + +0.7 3,300,620 - -0.7 3,300,708 + -0.3 3,300,708 + -0.6 3,300,851 - -0.6 3,300,874 - -1.1 3,300,909 + +0.5 3,300,909 + +0.4 3,300,917 - +0.8 3,300,961 + -0.8 3,301,118 + +0.0 3,301,124 + -0.3 3,301,124 + -0.2 3,301,124 + -0.0 3,301,125 - +0.6 3,301,229 - -1.0 3,301,229 - -0.2 3,301,229 - -0.3 3,301,252 + +0.1 3,301,253 - -0.1 3,301,372 - -0.4 3,301,382 - -0.7 3,301,385 + +0.8 3,301,385 + +1.5 3,301,386 - +0.2 3,301,386 - +0.1 3,301,406 + -0.0 3,301,474 - -0.2 3,301,534 + CA265_RS13295 0.11 -0.7 3,301,552 + CA265_RS13295 0.12 -0.1 3,301,617 - CA265_RS13295 0.15 -0.3 3,301,678 + CA265_RS13295 0.17 -0.2 3,301,678 + CA265_RS13295 0.17 +0.2 3,301,784 - CA265_RS13295 0.21 -0.2 3,301,784 - CA265_RS13295 0.21 -0.3 3,301,825 - CA265_RS13295 0.23 -0.1 3,301,862 + CA265_RS13295 0.25 -0.2 3,301,862 + CA265_RS13295 0.25 +0.0 3,301,918 - CA265_RS13295 0.27 +0.1 3,301,939 + CA265_RS13295 0.28 +0.7 3,301,940 - CA265_RS13295 0.28 +0.3 3,301,941 + CA265_RS13295 0.28 +0.3 3,302,012 - CA265_RS13295 0.31 -0.0 3,302,090 + CA265_RS13295 0.34 -0.3 3,302,135 - CA265_RS13295 0.36 +0.2 3,302,135 - CA265_RS13295 0.36 +0.1 3,302,157 + CA265_RS13295 0.36 -0.1 3,302,194 + CA265_RS13295 0.38 +0.9 3,302,227 + CA265_RS13295 0.39 +0.9 3,302,289 - CA265_RS13295 0.42 +0.3 3,302,301 + CA265_RS13295 0.42 +0.1 3,302,302 - CA265_RS13295 0.42 +0.7 3,302,304 - CA265_RS13295 0.42 -1.5 3,302,359 + CA265_RS13295 0.44 +0.3 3,302,364 + CA265_RS13295 0.45 +0.1 3,302,377 + CA265_RS13295 0.45 -0.8 3,302,377 + CA265_RS13295 0.45 -0.4 3,302,377 + CA265_RS13295 0.45 -0.6 3,302,378 - CA265_RS13295 0.45 +1.1 3,302,436 + CA265_RS13295 0.48 +0.1 3,302,436 + CA265_RS13295 0.48 +0.4 3,302,436 + CA265_RS13295 0.48 -0.0 3,302,437 - CA265_RS13295 0.48 +0.3 3,302,452 + CA265_RS13295 0.48 -0.4 3,302,453 - CA265_RS13295 0.48 -1.0 3,302,456 + CA265_RS13295 0.48 -0.1 3,302,467 - CA265_RS13295 0.49 -0.6 3,302,537 + CA265_RS13295 0.52 +0.6 3,302,545 + CA265_RS13295 0.52 +0.5 3,302,545 + CA265_RS13295 0.52 -0.1 3,302,549 + CA265_RS13295 0.52 -0.2 3,302,550 - CA265_RS13295 0.52 -0.1 3,302,640 - CA265_RS13295 0.56 +0.3 3,302,642 + CA265_RS13295 0.56 -0.1 3,302,654 + CA265_RS13295 0.56 -0.4 3,302,669 + CA265_RS13295 0.57 +0.4 3,302,670 - CA265_RS13295 0.57 -0.3 3,302,685 + CA265_RS13295 0.58 +0.3 3,302,722 - CA265_RS13295 0.59 +1.0 3,302,750 + CA265_RS13295 0.60 -0.2 3,302,750 + CA265_RS13295 0.60 +0.0 3,302,783 + CA265_RS13295 0.62 +1.0 3,302,867 - CA265_RS13295 0.65 +0.2 3,302,876 + CA265_RS13295 0.65 +0.9 3,302,876 + CA265_RS13295 0.65 -0.1 3,302,877 - CA265_RS13295 0.65 +0.1 3,302,877 - CA265_RS13295 0.65 -0.7 3,302,877 - CA265_RS13295 0.65 -0.1 3,302,936 - CA265_RS13295 0.68 +0.4 3,303,015 - CA265_RS13295 0.71 -0.7 3,303,099 - CA265_RS13295 0.74 +0.0 3,303,114 + CA265_RS13295 0.75 +0.1 3,303,139 + CA265_RS13295 0.76 +0.7 3,303,139 + CA265_RS13295 0.76 -0.4 3,303,139 + CA265_RS13295 0.76 -0.9 3,303,144 + CA265_RS13295 0.76 +0.5 3,303,264 + CA265_RS13295 0.81 -0.4 3,303,300 + CA265_RS13295 0.82 -0.5 3,303,302 + CA265_RS13295 0.82 +0.5 3,303,303 - CA265_RS13295 0.82 -0.7 3,303,303 - CA265_RS13295 0.82 -0.4 3,303,303 - CA265_RS13295 0.82 -0.5 3,303,366 + CA265_RS13295 0.85 +0.1 3,303,407 + CA265_RS13295 0.87 +0.4 3,303,408 - CA265_RS13295 0.87 -0.4 3,303,411 - CA265_RS13295 0.87 -0.6 3,303,441 - CA265_RS13295 0.88 -0.0 3,303,443 - CA265_RS13295 0.88 -0.8 3,303,473 + CA265_RS13295 0.89 -0.4 3,303,532 - -0.1 3,303,533 + -0.1 3,303,600 + -0.1 3,303,601 - -0.8 3,303,644 + -0.3 3,303,677 + +0.5 3,303,677 + -0.1 3,303,692 + +0.6 3,303,740 + -0.4 3,303,761 + +0.6 3,303,761 + +0.4 3,303,762 - +0.3 3,303,814 + -0.9 3,303,853 - +0.3 3,303,853 - -0.5 3,303,911 - CA265_RS13300 0.15 -0.9 3,303,972 + CA265_RS13300 0.20 +0.4 3,304,024 + CA265_RS13300 0.25 +0.5 3,304,024 + CA265_RS13300 0.25 -1.1 3,304,024 + CA265_RS13300 0.25 +0.3 3,304,025 - CA265_RS13300 0.25 +0.1 3,304,025 - CA265_RS13300 0.25 -0.3 3,304,025 - CA265_RS13300 0.25 +0.3 3,304,025 - CA265_RS13300 0.25 +0.3 3,304,025 - CA265_RS13300 0.25 -0.5 3,304,029 - CA265_RS13300 0.25 +0.5 3,304,038 + CA265_RS13300 0.26 -0.2 3,304,038 + CA265_RS13300 0.26 -1.6 3,304,038 + CA265_RS13300 0.26 -0.6 3,304,039 - CA265_RS13300 0.26 -0.3 3,304,039 - CA265_RS13300 0.26 -0.6 3,304,039 - CA265_RS13300 0.26 +1.0 3,304,043 - CA265_RS13300 0.26 -0.5 3,304,043 - CA265_RS13300 0.26 -0.1 3,304,043 - CA265_RS13300 0.26 -1.1 3,304,057 - CA265_RS13300 0.28 -1.2 3,304,057 - CA265_RS13300 0.28 -1.0 3,304,182 + CA265_RS13300 0.39 -0.0 3,304,228 - CA265_RS13300 0.43 +0.7 3,304,300 + CA265_RS13300 0.49 -0.3 3,304,498 + CA265_RS13300 0.66 +0.2 3,304,498 + CA265_RS13300 0.66 -0.0 3,304,499 - CA265_RS13300 0.66 +0.4 3,304,499 - CA265_RS13300 0.66 -0.0 3,304,499 - CA265_RS13300 0.66 +0.5 3,304,499 - CA265_RS13300 0.66 -0.0 3,304,499 - CA265_RS13300 0.66 -0.5 3,304,502 - CA265_RS13300 0.67 -0.3 3,304,529 + CA265_RS13300 0.69 -0.3 3,304,530 - CA265_RS13300 0.69 -0.6 3,304,568 - CA265_RS13300 0.72 +0.2 3,304,575 - CA265_RS13300 0.73 -0.3 3,304,605 - CA265_RS13300 0.76 -0.6 3,304,608 - CA265_RS13300 0.76 +0.5 3,304,651 - CA265_RS13300 0.80 -0.6 3,304,693 - CA265_RS13300 0.83 -1.0 3,304,693 - CA265_RS13300 0.83 -1.6 3,304,709 + CA265_RS13300 0.85 +0.1 3,304,709 + CA265_RS13300 0.85 +0.7
Or see this region's nucleotide sequence