Experiment: R2A with Chloramphenicol 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS09635 and CA265_RS09640 are separated by 109 nucleotides CA265_RS09640 and CA265_RS09645 are separated by 105 nucleotides CA265_RS09645 and CA265_RS09650 are separated by 71 nucleotides CA265_RS09650 and CA265_RS09655 are separated by 318 nucleotides
CA265_RS09635: CA265_RS09635 - GxxExxY protein, at 2,381,179 to 2,381,579
_RS09635
CA265_RS09640: CA265_RS09640 - ATPase, at 2,381,689 to 2,382,066
_RS09640
CA265_RS09645: CA265_RS09645 - hypothetical protein, at 2,382,172 to 2,382,384
_RS09645
CA265_RS09650: CA265_RS09650 - diaminopimelate decarboxylase, at 2,382,456 to 2,383,628
_RS09650
CA265_RS09655: CA265_RS09655 - aspartate kinase, at 2,383,947 to 2,385,260
_RS09655
Position (kb)
2382
2383
2384 Strain fitness (log2 ratio)
-2
-1
0
1 at 2381.464 kb on + strand, within CA265_RS09635 at 2381.469 kb on + strand, within CA265_RS09635 at 2381.550 kb on - strand at 2381.555 kb on + strand at 2381.600 kb on + strand at 2381.605 kb on - strand at 2381.610 kb on + strand at 2381.710 kb on + strand at 2381.749 kb on - strand, within CA265_RS09640 at 2381.919 kb on + strand, within CA265_RS09640 at 2381.963 kb on + strand, within CA265_RS09640 at 2382.142 kb on - strand at 2382.152 kb on + strand at 2382.159 kb on - strand at 2382.194 kb on + strand, within CA265_RS09645 at 2382.195 kb on - strand, within CA265_RS09645 at 2382.195 kb on - strand, within CA265_RS09645 at 2382.198 kb on - strand, within CA265_RS09645 at 2382.238 kb on - strand, within CA265_RS09645 at 2382.284 kb on + strand, within CA265_RS09645 at 2382.284 kb on + strand, within CA265_RS09645 at 2382.285 kb on - strand, within CA265_RS09645 at 2382.322 kb on + strand, within CA265_RS09645 at 2382.457 kb on + strand at 2382.457 kb on + strand at 2382.582 kb on + strand, within CA265_RS09650 at 2382.802 kb on - strand, within CA265_RS09650 at 2382.986 kb on + strand, within CA265_RS09650 at 2383.110 kb on + strand, within CA265_RS09650 at 2383.294 kb on - strand, within CA265_RS09650 at 2383.298 kb on + strand, within CA265_RS09650 at 2383.495 kb on + strand, within CA265_RS09650 at 2383.570 kb on + strand at 2383.675 kb on + strand at 2383.727 kb on - strand at 2383.802 kb on - strand at 2383.813 kb on + strand at 2383.814 kb on - strand at 2383.845 kb on + strand at 2383.846 kb on - strand at 2383.846 kb on - strand at 2383.880 kb on - strand at 2383.948 kb on + strand at 2383.961 kb on + strand at 2383.992 kb on - strand at 2384.024 kb on - strand at 2384.024 kb on - strand at 2384.135 kb on + strand, within CA265_RS09655 at 2384.136 kb on - strand, within CA265_RS09655 at 2384.136 kb on - strand, within CA265_RS09655 at 2384.148 kb on - strand, within CA265_RS09655 at 2384.157 kb on + strand, within CA265_RS09655 at 2384.196 kb on + strand, within CA265_RS09655 at 2384.247 kb on - strand, within CA265_RS09655 at 2384.252 kb on + strand at 2384.269 kb on + strand, within CA265_RS09655 at 2384.298 kb on + strand, within CA265_RS09655 at 2384.298 kb on + strand, within CA265_RS09655 at 2384.299 kb on - strand, within CA265_RS09655 at 2384.299 kb on - strand, within CA265_RS09655 at 2384.321 kb on + strand, within CA265_RS09655 at 2384.321 kb on + strand, within CA265_RS09655 at 2384.322 kb on - strand, within CA265_RS09655 at 2384.375 kb on + strand, within CA265_RS09655 at 2384.512 kb on + strand, within CA265_RS09655
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloramphenicol 0.005 mg/ml remove 2,381,464 + CA265_RS09635 0.71 +0.5 2,381,469 + CA265_RS09635 0.72 -0.1 2,381,550 - -0.6 2,381,555 + +0.5 2,381,600 + +0.1 2,381,605 - +0.8 2,381,610 + +0.2 2,381,710 + -0.3 2,381,749 - CA265_RS09640 0.16 +0.3 2,381,919 + CA265_RS09640 0.61 -0.7 2,381,963 + CA265_RS09640 0.72 +0.4 2,382,142 - +0.4 2,382,152 + -0.6 2,382,159 - -0.3 2,382,194 + CA265_RS09645 0.10 -0.1 2,382,195 - CA265_RS09645 0.11 -0.6 2,382,195 - CA265_RS09645 0.11 +0.8 2,382,198 - CA265_RS09645 0.12 +0.1 2,382,238 - CA265_RS09645 0.31 -0.5 2,382,284 + CA265_RS09645 0.53 +0.9 2,382,284 + CA265_RS09645 0.53 -0.9 2,382,285 - CA265_RS09645 0.53 -0.2 2,382,322 + CA265_RS09645 0.70 +0.4 2,382,457 + +0.1 2,382,457 + +0.3 2,382,582 + CA265_RS09650 0.11 -0.3 2,382,802 - CA265_RS09650 0.29 -0.2 2,382,986 + CA265_RS09650 0.45 +0.3 2,383,110 + CA265_RS09650 0.56 +0.6 2,383,294 - CA265_RS09650 0.71 -1.7 2,383,298 + CA265_RS09650 0.72 +1.5 2,383,495 + CA265_RS09650 0.89 +1.6 2,383,570 + -1.1 2,383,675 + -0.1 2,383,727 - -0.5 2,383,802 - +0.2 2,383,813 + +0.9 2,383,814 - -0.6 2,383,845 + -0.0 2,383,846 - -0.0 2,383,846 - -1.9 2,383,880 - -0.4 2,383,948 + -0.2 2,383,961 + -0.9 2,383,992 - +0.3 2,384,024 - +0.2 2,384,024 - -0.6 2,384,135 + CA265_RS09655 0.14 +1.2 2,384,136 - CA265_RS09655 0.14 -0.1 2,384,136 - CA265_RS09655 0.14 +1.5 2,384,148 - CA265_RS09655 0.15 -0.7 2,384,157 + CA265_RS09655 0.16 +0.1 2,384,196 + CA265_RS09655 0.19 +0.6 2,384,247 - CA265_RS09655 0.23 -0.1 2,384,252 + +0.3 2,384,269 + CA265_RS09655 0.25 +0.3 2,384,298 + CA265_RS09655 0.27 +0.0 2,384,298 + CA265_RS09655 0.27 -0.0 2,384,299 - CA265_RS09655 0.27 -0.0 2,384,299 - CA265_RS09655 0.27 +0.1 2,384,321 + CA265_RS09655 0.28 -0.5 2,384,321 + CA265_RS09655 0.28 +0.3 2,384,322 - CA265_RS09655 0.29 -0.3 2,384,375 + CA265_RS09655 0.33 +0.5 2,384,512 + CA265_RS09655 0.43 +1.0
Or see this region's nucleotide sequence