Experiment: R2A with Chloramphenicol 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS02980 and CA265_RS02985 are separated by 176 nucleotides CA265_RS02985 and CA265_RS02990 are separated by 261 nucleotides CA265_RS02990 and CA265_RS02995 are separated by 79 nucleotides
CA265_RS02980: CA265_RS02980 - hypothetical protein, at 743,206 to 743,757
_RS02980
CA265_RS02985: CA265_RS02985 - hypothetical protein, at 743,934 to 744,488
_RS02985
CA265_RS02990: CA265_RS02990 - mannosyltransferase, at 744,750 to 745,859
_RS02990
CA265_RS02995: CA265_RS02995 - 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase, at 745,939 to 746,760
_RS02995
Position (kb)
744
745
746 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 743.760 kb on - strand at 743.793 kb on - strand at 743.799 kb on - strand at 743.802 kb on + strand at 743.803 kb on - strand at 743.827 kb on - strand at 743.899 kb on + strand at 743.903 kb on + strand at 743.984 kb on - strand at 743.993 kb on + strand, within CA265_RS02985 at 743.994 kb on - strand, within CA265_RS02985 at 744.076 kb on + strand, within CA265_RS02985 at 744.096 kb on - strand, within CA265_RS02985 at 744.222 kb on + strand, within CA265_RS02985 at 744.222 kb on + strand, within CA265_RS02985 at 744.235 kb on - strand, within CA265_RS02985 at 744.238 kb on - strand, within CA265_RS02985 at 744.238 kb on - strand, within CA265_RS02985 at 744.300 kb on + strand, within CA265_RS02985 at 744.301 kb on - strand, within CA265_RS02985 at 744.301 kb on - strand, within CA265_RS02985 at 744.315 kb on - strand, within CA265_RS02985 at 744.315 kb on - strand, within CA265_RS02985 at 744.330 kb on - strand, within CA265_RS02985 at 744.429 kb on + strand, within CA265_RS02985 at 744.430 kb on - strand, within CA265_RS02985 at 744.440 kb on - strand at 744.440 kb on - strand at 744.448 kb on + strand at 744.473 kb on + strand at 744.539 kb on - strand at 744.602 kb on + strand at 744.637 kb on - strand at 744.637 kb on - strand at 744.637 kb on - strand at 744.639 kb on + strand at 744.640 kb on - strand at 744.647 kb on - strand at 744.747 kb on + strand at 744.913 kb on + strand, within CA265_RS02990 at 744.913 kb on + strand, within CA265_RS02990 at 744.914 kb on - strand, within CA265_RS02990 at 744.915 kb on + strand, within CA265_RS02990 at 744.982 kb on + strand, within CA265_RS02990 at 744.983 kb on - strand, within CA265_RS02990 at 745.054 kb on + strand, within CA265_RS02990 at 745.096 kb on - strand, within CA265_RS02990 at 745.126 kb on - strand, within CA265_RS02990 at 745.260 kb on + strand, within CA265_RS02990 at 745.265 kb on - strand, within CA265_RS02990 at 745.328 kb on + strand, within CA265_RS02990 at 745.352 kb on - strand, within CA265_RS02990 at 745.493 kb on + strand, within CA265_RS02990 at 745.498 kb on + strand, within CA265_RS02990 at 745.498 kb on + strand, within CA265_RS02990 at 745.521 kb on + strand, within CA265_RS02990 at 745.522 kb on - strand, within CA265_RS02990 at 745.557 kb on - strand, within CA265_RS02990 at 745.564 kb on - strand, within CA265_RS02990 at 745.677 kb on - strand, within CA265_RS02990 at 745.729 kb on - strand at 745.801 kb on + strand at 745.810 kb on - strand at 745.851 kb on + strand at 745.852 kb on - strand at 745.866 kb on + strand at 745.887 kb on + strand at 745.888 kb on - strand at 745.888 kb on - strand at 745.889 kb on + strand at 745.940 kb on + strand at 745.954 kb on - strand at 746.853 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Chloramphenicol 0.005 mg/ml remove 743,760 - +0.6 743,793 - -0.6 743,799 - -0.4 743,802 + -1.3 743,803 - -0.4 743,827 - -0.9 743,899 + +0.7 743,903 + -0.3 743,984 - +0.3 743,993 + CA265_RS02985 0.11 +0.4 743,994 - CA265_RS02985 0.11 +0.4 744,076 + CA265_RS02985 0.26 +0.4 744,096 - CA265_RS02985 0.29 -0.1 744,222 + CA265_RS02985 0.52 -0.1 744,222 + CA265_RS02985 0.52 +0.5 744,235 - CA265_RS02985 0.54 +0.3 744,238 - CA265_RS02985 0.55 +0.7 744,238 - CA265_RS02985 0.55 -0.2 744,300 + CA265_RS02985 0.66 -0.1 744,301 - CA265_RS02985 0.66 +0.2 744,301 - CA265_RS02985 0.66 +1.0 744,315 - CA265_RS02985 0.69 +0.0 744,315 - CA265_RS02985 0.69 -0.3 744,330 - CA265_RS02985 0.71 +0.3 744,429 + CA265_RS02985 0.89 -1.5 744,430 - CA265_RS02985 0.89 -0.2 744,440 - -0.1 744,440 - -0.1 744,448 + +0.1 744,473 + +2.3 744,539 - +0.3 744,602 + +1.5 744,637 - +0.9 744,637 - -1.0 744,637 - -0.8 744,639 + +1.1 744,640 - +0.1 744,647 - -0.9 744,747 + -0.3 744,913 + CA265_RS02990 0.15 -1.7 744,913 + CA265_RS02990 0.15 +0.3 744,914 - CA265_RS02990 0.15 -0.7 744,915 + CA265_RS02990 0.15 -1.9 744,982 + CA265_RS02990 0.21 +0.3 744,983 - CA265_RS02990 0.21 -0.5 745,054 + CA265_RS02990 0.27 -1.1 745,096 - CA265_RS02990 0.31 -1.0 745,126 - CA265_RS02990 0.34 -2.2 745,260 + CA265_RS02990 0.46 -2.1 745,265 - CA265_RS02990 0.46 -1.2 745,328 + CA265_RS02990 0.52 -0.8 745,352 - CA265_RS02990 0.54 -0.5 745,493 + CA265_RS02990 0.67 -1.1 745,498 + CA265_RS02990 0.67 -2.6 745,498 + CA265_RS02990 0.67 -3.4 745,521 + CA265_RS02990 0.69 -1.7 745,522 - CA265_RS02990 0.70 -1.6 745,557 - CA265_RS02990 0.73 -0.2 745,564 - CA265_RS02990 0.73 -1.4 745,677 - CA265_RS02990 0.84 -1.5 745,729 - -0.1 745,801 + -1.9 745,810 - -3.0 745,851 + -0.6 745,852 - -1.2 745,866 + +2.5 745,887 + -2.1 745,888 - -0.6 745,888 - +0.5 745,889 + -0.2 745,940 + +0.3 745,954 - -0.1 746,853 + -2.0
Or see this region's nucleotide sequence